The effect of acute exercise on attentional control and theta power in young adults.

Exercise has a profound impact on one's health, and it is becoming increasingly accepted that exercise also benefits cognitive functioning. Yet, the neural mechanism for which cognitive enhancement occurs is less understood. Therefore, the purpose of our study was to experimentally test whether an acute exercise activity was able to increase theta power and behavioral performance during an executive functioning attentional control task. Participants were randomly assigned to either a stationary-bike exercise or a resting control condition. Thereafter, they completed the Eriksen flanker task, and most participants completed this while EEG data were recorded. From the flanker task data, we demonstrated an interaction effect from both accuracy and reaction time measurements. Importantly, the exercise group was more accurate than the control group in incongruent trials. From the EEG data, theta power was overall higher in the exercise group, especially during the congruent trials, compared to controls. Our results add to the limited but growing body of research that suggests acute exercise produces a general increase in theta power, which in turn may play a role in enhancing cognitive performance. These results, combined with previous research, could have widespread implications in multiple settings such as in the investigation of a biomarker of physical fitness, neurorehabilitation, and in education.

Climate Change, Air Pollution, and Physical Inactivity: Is Active Transportation Part of the Solution?

Active transportation is defined as self-propelled, human-powered transportation modes, such as walking and bicycling. In this article, we review the evidence that reliance on gasoline-powered transportation is contributing to global climate change, air pollution, and physical inactivity and that this is harmful to human health. Global climate change poses a major threat to human health and in the future could offset the health gains achieved over the last 100 yr. Based on hundreds of scientific studies, there is strong evidence that human-caused greenhouse gas emissions are contributing to global climate change. Climate change is associated with increased severity of storms, flooding, rising sea levels, hotter climates, and drought, all leading to increased morbidity and mortality. Along with increases in atmospheric CO2, other pollutants such as nitrogen dioxide, ozone, and particulate matter (e.g., PM2.5) are released by combustion engines and industry, which can lead to pulmonary and cardiovascular diseases. Also, as car ownership and vehicle miles traveled have increased, the shift toward motorized transport has contributed to physical inactivity. Each of these global challenges has resulted in, or is projected to result in, millions of premature deaths each year. One of the ways that nations can mitigate the health consequences of climate change, air pollution, and chronic diseases is through the use of active transportation. Research indicates that populations that rely heavily on active transportation enjoy better health and increased longevity. In summary, active transportation has tremendous potential to simultaneously address three global public health challenges of the 21st century.

IFN-γ and IL-17A regulate intestinal crypt production of CXCL10 in the healthy and inflamed colon.

During intestinal inflammation, immature cells within the intestinal crypt are called upon to replenish lost epithelial cell populations, promote tissue regeneration, and restore barrier integrity. Inflammatory mediators including TH1/TH17-associated cytokines influence tissue health and regenerative processes, yet how these cytokines directly influence the colon crypt epithelium and whether the crypt remains responsive to these cytokines during active damage and repair, remain unclear. Here, using laser-capture microdissection and primary colon organoid culture, we show that the cytokine milieu regulates the ability of the colonic crypt epithelium to participate in proinflammatory signaling. IFN-γ induces the TH1-recruiting, proinflammatory chemokine CXCL10/IP10 in primary murine intestinal crypt epithelium. CXCL10 was also induced in colonic organoids derived from mice with active, experimentally induced colitis, suggesting that the crypt can actively secrete CXCL10 in select cytokine environments during colitis. Colon expression of cxcl10 further increased during infectious and noninfectious colitis in Il17a-/- mice, demonstrating that IL-17A exerts a negative effect on CXCL10 in vivo. Furthermore, IL-17A directly antagonized CXCL10 production in ex vivo organoid cultures derived from healthy murine colons. Interestingly, direct antagonism of CXCL10 was not observed in organoids derived from colitic mouse colons bearing active lesions. These data, highlighting the complex interplay between the cytokine milieu and crypt epithelia, demonstrate proinflammatory chemokines can be induced within the colonic crypt and suggest the crypt remains responsive to cytokine modulation during inflammation.NEW & NOTEWORTHY Upon damage, the intestinal epithelium regenerates to restore barrier function. Here we observe that the local colonic cytokine milieu controls the production of procolitic chemokines within the crypt base and colon crypts remain responsive to cytokines during inflammation. IFN-γ promotes, while IL-17 antagonizes, CXCL10 production in healthy colonic crypts, while responses to cytokines differ in inflamed colon epithelium. These data reveal novel insight into colon crypt responses and inflammation-relevant alterations in signaling.

The Effect of a Curved Non-Motorized Treadmill on Running Gait Length, Imbalance and Stride Angle.

Running on a non-motorized, curved-deck treadmill is thought to improve gait mechanics. It is not known, though, whether the change in gait carries over to running on a motorized treadmill on level ground. To determine the effect of running on a curved non-motorized treadmill (CNT) on gait characteristics, measured during a subsequent bout of running on a traditional motorized treadmill (TMT), sixteen healthy college-aged participants, aged (mean ± SD) 20.4 ± 1.6 years, volunteered to have their gait analyzed while running on a TMT and CNT. After familiarization with, and a warm-up on, both treadmills, each subject completed five 4-min bouts of running, alternating between traditional motorized and curved non-motorized treadmills: TMT-1, CNT-1, TMT-2, CNT-2, and TMT-3. Variables of interest included step length (m), stride length (m), imbalance score (%), and stride angle (°), and were measured using Optogait gait analysis equipment. We found differences in gait characteristics among TMT-1, TMT-2, and TMT-3, which can be attributed to running on the CNT. The results show that running on a CNT resulted in significant changes in gait characteristics (step length, stride length, imbalance score and stride angle). These findings suggest that running on a CNT can significantly influence running gait.

Specifically Targeted Electromagnetic Fields Arrest Proliferation of Glioblastoma Multiforme U-87 Cells in Culture.

BACKGROUND/AIM: Glioblastoma multiforme is an aggressive primary tumor that arises in the glial cells of the brain. Standardized first-line treatment has considerable morbidity and less than one-year median survival after intervention. Ultra-low intensity electromagnetic fields have been shown to interact with biological organisms without anticipated deleterious side-effects. The aim of the study was to determine if a novel, non-invasive application of non-ionizing radiation has an inhibitory effect on proliferation of glioblastoma multiforme cells. MATERIALS AND METHODS: U-87 MG cells were continuously exposed for 54 h to an electromagnetic field tuned to simultaneously interact with DNA/RNA oligonucleotides (mutated alpha-kinase 2 gene/Hsa-miR-381-5p respectively) and proteins (HSP70/CHI3L1). RESULTS: Exposed cells demonstrated a significant inhibition of cell growth and concurrent increase in cell death. CONCLUSION: This technology induces cell death by novel non-cytotoxic mechanisms unlikely to induce side-effects in patients; can be customized for individual tumors and may contribute to the emerging strategy of personalized medicine.

Portable open-circuit spirometry systems.

BACKGROUND: The purpose of this review is to describe the evolution of portable open-circuit spirometry systems, and discuss their validity, reliability, and principles of operation. METHODS: Eleven devices were selected for review: the Oxylog, Aerosport KB1-C, Cosmed K2, Cosmed K4RQ, Cosmed K4b2, MetaMax I, MetaMax II, Metamax3B/VmaxST, Medgraphics VO2000, Oxycon Mobile I and Oxycon Mobile II. The validity (compared to the Douglas bag method [DBM]) and reliability of each device for measuring VO2 was summarized. RESULTS: Mean differences in resting measurements of VO2 were within ±0.05 L/min for all devices except one (difference of 0.17 L/min). When compared to the DBM, VO2 differences for all devices ranged from 0.01 L/min to 0.29 L/min during submaximal intensity exercise and from 0.01 L/min to 0.36 L/min during vigorous/maximal intensity. During submaximal and maximal intensities, ICC ranged from 0.66-0.99 and CV ranged from 2.0-14.2%. Of these devices, four used breath-by-breath technology and six used micro-proportional sampling technology. Validity and reliability of devices did not seem to differ between methods of gas collection. CONCLUSIONS: Of the three commercially available devices in 2015, all were found to be reliable. Two of the three systems (Cosmed K4b2 and Oxycon Mobile II) provided valid estimates of VO2 (mean values within ±0.10 L/min of DBM) during rest, and submaximal and maximal intensities, while the MetaMax3B slightly overestimated VO2, particularly at maximal exercise.

Inhibition of inflammatory gene expression in keratinocytes using a composition containing carnitine, thioctic Acid and saw palmetto extract.

Chronic inflammation of the hair follicle (HF) is considered a contributing factor in the pathogenesis of androgenetic alopecia (AGA). Previously, we clinically tested liposterolic extract of Serenoa repens (LSESr) and its glycoside, ß-sitosterol, in subjects with AGA and showed a highly positive response to treatment. In this study, we sought to determine whether blockade of inflammation using a composition containing LSESr as well as two anti-inflammatory agents (carnitine and thioctic acid) could alter the expression of molecular markers of inflammation in a well-established in vitro system. Using a well-validated assay representative of HF keratinocytes, specifically, stimulation of cultured human keratinocyte cells in vitro, we measured changes in gene expression of a spectrum of well-known inflammatory markers. Lipopolysaccharide (LPS) provided an inflammatory stimulus. In particular, we found that the composition effectively suppressed LPS-activated gene expression of chemokines, including CCL17, CXCL6 and LTB(4) associated with pathways involved in inflammation and apoptosis. Our data support the hypothesis that the test compound exhibits anti-inflammatory characteristics in a well-established in vitro assay representing HF keratinocyte gene expression. These findings suggest that 5-alpha reductase inhibitors combined with blockade of inflammatory processes could represent a novel two-pronged approach in the treatment of AGA with improved efficacy over current modalities.

Effect of the E3 Fitness Grips on Running Economy.

The purpose of this study was to evaluate the effect of the E3 Fitness Grips (BioGrip, Inc., Rancho Cordova, CA) on running economy, as measured by oxygen uptake (VO2), and heart rate (HR) during submaximal treadmill running. Eleven subjects, seven female and four male, completed a submaximal running test on a treadmill while VO2 and HR were measured continuously. After achieving steady-state at a speed and grade that elicited a VO2 equivalent to 70% VO2max, the subjects ran for five minutes holding the E3 Fitness Grips (G) and five minutes without the grips (NG). The tests were counterbalanced so half of the subjects held the grips first and half completed the NG condition first. The difference in VO2 and HR between the G and NG conditions were compared to determine the effect on running economy. The mean VO2 (33.2±4.6 vs. 33.2±4.6 ml·kg-1·min-1, p=0.96) and mean HR (172.0±8.9 vs. 172.8±8.9 beats·min-1, p=0.38) were not significantly different between the G and NG conditions during submaximal running. These findings suggest that the E3 Fitness Grips do not significantly alter running economy, as measured by VO2, or HR during submaximal treadmill running.

A preliminary study of one year of pedometer self-monitoring.

BACKGROUND: Long-term pedometer monitoring has not been attempted. PURPOSE: The purpose of this project was to collect 365 days of continuous self-monitored pedometer data to explore the natural variability of physical activity. METHODS: Twenty-three participants (7 men, 16 women; M age = 38 +- 9.9 years; M body mass index = 27.7 +- 6.2 kg/m2) were recruited by word of mouth at two southern U.S. universities. Participants were asked to wear pedometers at their waist during waking hours and record steps per day and daily behaviors (e.g., sport/exercise, work or not) on a simple calendar. In total, participants wore pedometers and recorded 8,197 person-days of data (of a possible 8,395 person-days, or 98%) for a mean of 10,090 +- 3,389 steps/day. Missing values were estimated using the Missing Values Analysis EM function in SPSS, Version 11.0.1. RESULTS: A mean of 10,082 +- 3,319 steps/day was computed. Using the corrected data, differences in steps/day were significant for season (summer > winter, F = 7.57, p = .001), day of the week (weekday > weekend, F = 3.97, p = .011), type of day (workday vs. nonworkday, F = 9.467, p = .008), and participation in sport/exercise (day with sport/exercise > day without sport/exercise, F = 102.5, p < .0001). CONCLUSIONS: These data suggest that surveillance should be conducted in the spring/fall or that an appropriate correction factor should be considered if the intent is to capture values resembling the year-round average.

Tbx12 regulates eye development in Xenopus embryos.

The regulation of vertebrate eye development requires the activity of many transcription factors. In this report, we demonstrate that the T-box factor Tbx12 is necessary for normal development of the retina. Tbx12 is expressed during early stages of retinal development in multiple species of vertebrate embryos. We injected mRNAs encoding wild type and mutant forms of Tbx12 into Xenopus embryos. The Tbx12 injected embryos exhibit multiple defects in eye development including reduced eye size and disruption of normal retinal laminar organization. Tbx12 appears to function as a repressor of transcription during eye development. Our results indicate that Tbx12 activity is required for the proper generation and organization of retinal cells in the vertebrate eye.

Analysis of Fz10 expression in mouse embryos.

Wnt signaling molecules regulate the development of multiple organs in vertebrate embryos. We have isolated cDNA clones for frizzled10 (Fz10), which encodes a putative Wnt receptor, to further characterize the mechanisms of Wnt signaling in mouse embryos. Interestingly, Fz10 is expressed in the same regions as Wnt7a in the neural tube, limb buds, and Müllerian duct.

Developmental expression of the Xenopus laevis Tbx20 orthologue.

We have isolated the Xenopus orthologue of the T-box gene, Tbx20, and characterized its developmental expression profile. We show that Tbx20 is one of the earliest markers of heart tissue in Xenopus, and is expressed throughout all cardiac tissue during later stages of development. In addition, we also observe expression in the cement gland, the jugular vein, the lung bud, the cloacal aperture, rhombomeres 2, 4, 6 and 8, and in a subset of motor neurons.