RESUMEN
A rapid and sensitive RP-HPLC method with UV detection (244 nm) for routine analysis of montelukast sodium and ebastine in a pharmaceutical formulation (Ebast-M) was developed. Chromatography was performed with mobile phase containing a mixture of methanol:acetonitrile:ammonium acetate (80:10:10, % v/v/v), pH of mobile phase was adjusted 5.5 using glacial acetic acid and flow rate was 1.2 ml/min. The method was validated for linearity, accuracy, robustness and intermediate precision. The linearity was established over the concentration range of 0.01-0.06 mg/ml for both drugs. The correlation coefficients (r (2)) for ebastine and montelukast were 0.9989 and 0.9955, respectively. Statistical analysis of the data showed that the method was precise, accurate, reproducible and selective for the analysis of ebastine and montelukast drugs. The method was successfully employed for the determination of ebastine and montelukast in commercially available tablet dosage form.
RESUMEN
Hedyotis corymbosa Linn is spreading, suffruticose annual, belongs to family Rubiaceae frequently met with in field through out India, usually during rainy season. This plant is used for their medicinal properties as a folk medicine to treat jaundice, mouth wash in toothache. Hence the present study was aimed to investigate the hepatoprotective activity of ethanolic extract of Hedyotis corymbosa which was separated in to different fractions against carbon tetrachloride intoxification. The results indicate that, Intoxification with CCl(4) increase the levels of SGOT and SGPT. The elevated levels of SGOT and SGPTwere significantly decreased by ether and butanol fractions at P<0.001 and butanone and ethanol at p<0.05, where as petroleum ether and ethyl acetate did not shown any significant reduction in the level of SGOT and SGPT.
