[Justifying the use of primary tissue culture method for evaluation of sodium fluoride safety in nutrition products].

Toxicity of sodium fluoride added to nutrition products is assessed through peculiarities of kidney tissue culture growth in vitro--over 75% inhibition of cell clumps growth supports high toxic influence of sodium fluoride, the growth decrease from 63% to 74% signifies lower degree, the growth inhibition less than 62% demonstrates very low toxicity of the chemical.

[Effect of the serum of alcoholics on the growth of primary cultures human fetal liver cells]. / Vliianie syvorotki krovi bol'nykh alkogolizmom na rost pervichnykh kul'tur pechenochnykh kletok plodov cheloveka.

The study has aimed at ascertaining the role of the "serum factor" of alcoholism, the mechanism of its action on the development of liver lesions and morpho-functional properties of liver cells in alcoholics. The results obtained point to significant qualitative peculiarities of the growth of hepatocyte culture in the presence of alcoholics' serum which are expressed in suppression of liver cells and stimulation of white blood cells. The development of alcohol-related hepatic lesion (AHL) is known to be attended by mobilization of immunological mechanisms which is confirmed by the high lymphocytic activity in the considered model. Other leading signs of AHL is dystrophy of hepatocytes and proliferation of connective tissue which also manifests in a scissors-like difference in the growth of epithelium-like and fibroblast-like cells.

[Effect of alcoholic patients' serum on the reproductive activity of human embryo fibroblasts]. / Vliianie syvorotki krovi bol'nykh alkogolizmom na reproduktivnuiu aktivnost' fibroblastov émbrionov cheloveka.

Alcoholics' blood serum produces a greater stimulation of the growth of human embryonic cells as compared with the serum from healthy individuals. In the course of treatment, the serum stimulating influence on the growth of human embryonic cells tended to diminish, approaching that of control. The authors ascertained a statistically significant positive correlation between the intensity of the embryonic cells growth under the influence of blood serum from alcoholic patients on the one hand and some hematologic parameters of these subjects on the other. Sensitizing therapy with teturam had no substantial effect on either the biological properties of alcoholics' serum or on the reproductive activity of fibroblasts, whereas the administration of neuroleptics was associated with a statistically significant decrease in the proliferation of fibroblasts.

[Effect of conditions of cultivation on quantitative assessment of the viability of an organism's tissues]. / Vliianie uslovii kul'tivirovaniia na kolichestvennogo otsenku zhiznesposobnosti tkanei organizma.

Viability of mouse kidney tissue explants as estimated quantitatively according to their growth in plasmafree culture depends on some factors connected with cultivation conditions. Methods of serum preparation, pH of the medium, as well as peculiarities of tissue explants distribution in cultivation, their size and fastness of attachment to the substrate proved to influence the quantitative estimation of the tissue explants viability.

[Quantitative assessment of the viability of renal tissue when the donor's body is treated differently]. / Kolichestvennaia otsenka zhiznesposobnosti pochechnoi tkani pri razlichnykh vozdeistviiakh na organizm donora

It was shown that the vital capacity of the mouse kidney tissues quantitatively estimated by the in vitro growth in a plasma-free medium depended upon the condition of the donor organism. Depression of the culture growth was noted after the general X-irradiation of the animals, as well as following prolonged starvation and chloric cadmium poisoning. An increased growth of the kidney tissues was observed both in compensatory hypertrophy caused by unilateral nephrectomy and in subcutaneous inflammation.