RESUMEN
AIM: Regular and structured physical activity is known to be effective in preventing and/or reducing the physical and mental decline associated with aging. Indeed, such usefulness of physical activity has been associated with the concept of "successful aging". The aim of the present study was to evaluate the possible physical and cognitive effects deriving from the practice of Dancesport in comparison with the participation in adapted physical activity (APA) programs and sedentarity. METHODS: A total of 150 healthy older adults were enrolled, consisting of three groups: 1) Dancesport (non-competitive Latin American and Standard dancers); 2) APA (subjects practicing a multicomponent training program adapted to elderly); 3) control (sedentary subjects). All participants were assessed with cognitive computerized tests and underwent motor tests (Tinetti Test [TT] and Sit and Reach [SR] Test), and filled out a questionnaire to evaluate leisure cognitive activities and Short Form-12 (SF-12) questionnaire to assess quality of life. RESULTS: Subjects practicing Dancesport and APA performed significantly better in all proposed tests than sedentary subjects. In particular, dancers reported better scores in both cognitive and motor tests as well as in SF-12 compared to APA. CONCLUSION: Given its peculiar characteristics, Dancesport represents a feasible, attractive and alternative physical activity to preserve cognitive and physical functions during aging. Increased self-esteem, social contact and psychophysical wellness significantly ameliorate the quality of life during aging.
Asunto(s)
Envejecimiento/fisiología , Cognición , Baile/fisiología , Baile/psicología , Ejercicio Físico/fisiología , Ejercicio Físico/psicología , Anciano , Femenino , Humanos , Relaciones Interpersonales , Masculino , Destreza Motora/fisiología , Calidad de Vida , Autoimagen , Encuestas y CuestionariosRESUMEN
Together with race, stature and age, sex is a main component of the biological identity. Thanks to its proportional correlation with parts of the human body, sex can be evaluated form the skeleton. The most accurate approach to determine sex by bone size is based on os coxae or skull. After natural disaster their presence can never be guaranteed, therefore the development of methods of sex determination using other skeletal elements can result crucial. Herein, sexual dimorphism in the human scapula is used to develop a two-variable discriminant function for sex estimation. We have enrolled 100 males and 100 females who underwent thoracic CT scan evaluation and we have estimated two scapular diameters. The estimation has been carried out by analyzing images of the scapulae of each patient after three dimensional post-processing reconstructions. The two-variable function allows to obtain an overall accuracy of 88% on the calibration sample. Furthermore, we have employed the mentioned function on a collection of 10 individual test sample from the collection of the "Museo di Anatomia Umana di Firenze" of the Università degli Studi di Firenze; sex has been correctly predicted on 9 skeletons.
Asunto(s)
Escápula/anatomía & histología , Determinación del Sexo por el Esqueleto/métodos , Tomografía Computarizada por Rayos X , Anciano , Calibración , Análisis Discriminante , Femenino , Antropología Forense , Humanos , Imagenología Tridimensional , Masculino , Persona de Mediana Edad , Tamaño de los Órganos , Determinación del Sexo por el Esqueleto/normas , Población BlancaRESUMEN
Sphingosine-1-phosphate (S1P) is a lipid mediator, which affects many essential processes such as cell proliferation, differentiation and contraction in many cell types. We have previously demonstrated that the lipid mediator elicits Ca(2+) transients in a myoblastic cell line (C2C12) by interacting with its specific receptors (S1PR(s)). In the present study, we wanted to correlate the Ca(2+) response with activation of myoblastic cell contractility. C2C12 cells were first investigated for the expression and cellular organization of cytoskeletal proteins by immunoconfocal microscopy. We found that myoblasts exhibited a quite immature cytoskeleton, with filamentous actin dispersed as a web-like structure within the cytoplasm. To evaluate intracellular Ca(2+) mobilization, the cells were loaded with a fluorescent Ca(2+) indicator (Fluo-3), stimulated with S1P and simultaneously observed with differential interference contrast and fluorescence optics. Exogenous S1P-induced myoblastic cell contraction was temporally unrelated to S1P-induced intracellular Ca(2+) increase; cell contraction occurred within 5-8 s from stimulation, whereas intracellular Ca(2+) increase was evident only after 15-25 s. To support the Ca(2+) independence of myoblastic cell contraction, the cells were pretreated with a Ca(2+) chelator, BAPTA/AM, prior to stimulation with S1P. In these experimental conditions, the myoblasts were still able to contract, whereas the S1P-induced Ca(2+) transients were completely abolished. On the contrary, when C2C12 cells were induced to differentiate into skeletal myotubes, they responded to S1P with a rapid cell contraction concurrent with an increase in the intracellular Ca(2+). These data suggest that Ca(2+)-independent mechanism of cell contraction may be replaced by Ca(2+)-dependent ones during skeletal muscle differentiation.
Asunto(s)
Señalización del Calcio/efectos de los fármacos , Calcio/metabolismo , Ácido Egtácico/análogos & derivados , Lisofosfolípidos/farmacología , Contracción Muscular/efectos de los fármacos , Esfingosina/análogos & derivados , Esfingosina/farmacología , Actinas/efectos de los fármacos , Animales , Línea Celular , Tamaño de la Célula/efectos de los fármacos , Quelantes/farmacología , Citoesqueleto/efectos de los fármacos , Ácido Egtácico/farmacología , Técnica del Anticuerpo Fluorescente Indirecta , Colorantes Fluorescentes , Procesamiento de Imagen Asistido por Computador , Cinética , Ratones , Microscopía Confocal , MioblastosRESUMEN
The main anatomical resources found on the Internet are listed and discussed here, concerning the topics of gross anatomy, neuroanatomy, microscopic anatomy and anatomical museums. The search is updated to September 2000, and all the listed Web sites can be used to build a multimedial anatomical lab.