Evaluating the Accuracy of Using Fixed Ranges of METs to Categorize Exertional Intensity in a Heterogeneous Group of Healthy Individuals: Implications for Cardiorespiratory Fitness and Health Outcomes.

BACKGROUND: Appropriate quantification of exertional intensity remains elusive. OBJECTIVE: To compare, in a large and heterogeneous cohort of healthy females and males, the commonly used intensity classification system (i.e., light, moderate, vigorous, near-maximal) based on fixed ranges of metabolic equivalents (METs) to an individualized schema based on the exercise intensity domains (i.e., moderate, heavy, severe). METHODS: A heterogenous sample of 565 individuals (females 165; males 400; age range 18-83 years old) were included in the study. Individuals performed a ramp-incremental exercise test from which gas exchange threshold (GET), respiratory compensation point (RCP) and maximum oxygen uptake (VO2max) were determined to build the exercise intensity domain schema (moderate = METs ≤ GET; heavy = METs > GET but ≤ RCP; severe = METs > RCP) for each individual. Pearson's chi-square tests over contingency tables were used to evaluate frequency distribution within intensity domains at each MET value. A multi-level regression model was performed to identify predictors of the amplitude of the exercise intensity domains. RESULTS: A critical discrepancy existed between the confines of the exercise intensity domains and the commonly used fixed MET classification system. Overall, the upper limit of the moderate-intensity domain ranged between 2 and 13 METs and of the heavy-intensity domain between 3 and 18 METs, whereas the severe-intensity domain included METs from 4 onward. CONCLUSIONS: Findings show that the common practice of assigning fixed values of METs to relative categories of intensity risks misclassifications of the physiological stress imposed by exercise and physical activity. These misclassifications can lead to erroneous interpretations of the dose-response relationship of exercise and physical activity.

Measurement of a True [Formula: see text]O2max during a Ramp Incremental Test Is Not Confirmed by a Verification Phase.

The accuracy of an exhaustive ramp incremental (RI) test to determine maximal oxygen uptake ([Formula: see text]O2max) was recently questioned and the utilization of a verification phase proposed as a gold standard. This study compared the oxygen uptake ([Formula: see text]O2) during a RI test to that obtained during a verification phase aimed to confirm attainment of [Formula: see text]O2max. Sixty-one healthy males [31 older (O) 65 ± 5 yrs; 30 younger (Y) 25 ± 4 yrs] performed a RI test (15-20 W/min for O and 25 W/min for Y). At the end of the RI test, a 5-min recovery period was followed by a verification phase of constant load cycling to fatigue at either 85% (n = 16) or 105% (n = 45) of the peak power output obtained from the RI test. The highest [Formula: see text]O2 after the RI test (39.8 ± 11.5 mL·kg-1·min-1) and the verification phase (40.1 ± 11.2 mL·kg-1·min-1) were not different (p = 0.33) and they were highly correlated (r = 0.99; p < 0.01). This response was not affected by age or intensity of the verification phase. The Bland-Altman analysis revealed a very small absolute bias (-0.25 mL·kg-1·min-1, not different from 0) and a precision of ±1.56 mL·kg-1·min-1 between measures. This study indicated that a verification phase does not highlight an under-estimation of [Formula: see text]O2max derived from a RI test, in a large and heterogeneous group of healthy younger and older men naïve to laboratory testing procedures. Moreover, only minor within-individual differences were observed between the maximal [Formula: see text]O2 elicited during the RI and the verification phase. Thus a verification phase does not add any validation of the determination of a [Formula: see text]O2max. Therefore, the recommendation that a verification phase should become a gold standard procedure, although initially appealing, is not supported by the experimental data.

Using ramp-incremental V̇O2 responses for constant-intensity exercise selection.

Despite compelling evidence to the contrary, the view that oxygen uptake (V̇O2) increases linearly with exercise intensity (e.g., power output, speed) until reaching its maximum persists within the exercise physiology literature. This viewpoint implies that the V̇O2 response at any constant intensity is predictable from a ramp-incremental exercise test. However, the V̇O2 versus task-specific exercise intensity relationship constructed from ramp-incremental versus constant-intensity exercise are not equivalent preventing the use of V̇O2 responses from 1 domain to predict those of the other. Still, this "linear" translational framework continues to be adopted as the guiding principle for aerobic exercise prescription and there remains in the sport science literature a lack of understanding of how to interpret V̇O2 responses to ramp-incremental exercise and how to use those data to assign task-specific constant-intensity exercise. The objectives of this paper are to (i) review the factors that disassociate the V̇O2 versus exercise intensity relationship between ramp-incremental and constant-intensity exercise paradigms; (ii) identify when it is appropriate (or not) to use ramp V̇O2 responses to accurately assign constant-intensity exercise; and (iii) illustrate the technical and theoretical challenges with prescribing constant-intensity exercise solely on information acquired from ramp-incremental tests. Actual V̇O2 data collected during cycling exercise and V̇O2 kinetics modelling are presented to exemplify these concepts. Possible solutions to overcome these challenges are also presented to inform on appropriate intensity selection for individual-specific aerobic exercise prescription in both research and practical settings.

Slow V˙O2 kinetics in acute hypoxia are not related to a hyperventilation-induced hypocapnia.

We examined whether slower pulmonary O2 uptake (V˙O2p) kinetics in hypoxia is a consequence of: a) hypoxia alone (lowered arterial O2 pressure), b) hyperventilation-induced hypocapnia (lowered arterial CO2 pressure), or c) a combination of both. Eleven participants performed 3-5 repetitions of step-changes in cycle ergometer power output from 20W to 80% lactate threshold in the following conditions: i) normoxia (CON; room air); ii) hypoxia (HX, inspired O2 = 12%; lowered end-tidal O2 pressure [PETO2] and end-tidal CO2 pressure [PETCO2]); iii) hyperventilation (HV; increased PETO2 and lowered PETCO2); and iv) normocapnic hypoxia (NC-HX; lowered PETO2 and PETCO2 matched to CON). Ventilation was increased (relative to CON) and matched between HX, HV, and NC-HX conditions. During each condition VO2p˙ was measured and phase II V˙O2p kinetics were modeled with a mono-exponential function. The V˙O2p time constant was different (p < 0.05) amongst all conditions: CON, 26 ±â€¯11s; HV, 36 ±â€¯14s; HX, 46 ±â€¯14s; and NC-HX, 52 ±â€¯13s. Hypocapnia may prevent further slowing of V˙O2p kinetics in hypoxic exercise.

Formulation of evidence-based messages to promote the use of physical activity to prevent and manage Alzheimer's disease.

BACKGROUND: The impending public health impact of Alzheimer's disease is tremendous. Physical activity is a promising intervention for preventing and managing Alzheimer's disease. However, there is a lack of evidence-based public health messaging to support this position. This paper describes the application of the Appraisal of Guidelines Research and Evaluation II (AGREE-II) principles to formulate an evidence-based message to promote physical activity for the purposes of preventing and managing Alzheimer's disease. METHODS: A messaging statement was developed using the AGREE-II instrument as guidance. Methods included (a) conducting a systematic review of reviews summarizing research on physical activity to prevent and manage Alzheimer's disease, and (b) engaging stakeholders to deliberate the evidence and formulate the messaging statement. RESULTS: The evidence base consisted of seven systematic reviews focused on Alzheimer's disease prevention and 20 reviews focused on symptom management. Virtually all of the reviews of symptom management conflated patients with Alzheimer's disease and patients with other dementias, and this limitation was reflected in the second part of the messaging statement. After deliberating the evidence base, an expert panel achieved consensus on the following statement: "Regular participation in physical activity is associated with a reduced risk of developing Alzheimer's disease. Among older adults with Alzheimer's disease and other dementias, regular physical activity can improve performance of activities of daily living and mobility, and may improve general cognition and balance." The statement was rated favourably by a sample of older adults and physicians who treat Alzheimer's disease patients in terms of its appropriateness, utility, and clarity. CONCLUSION: Public health and other organizations that promote physical activity, health and well-being to older adults are encouraged to use the evidence-based statement in their programs and resources. Researchers, clinicians, people with Alzheimer's disease and caregivers are encouraged to adopt the messaging statement and the recommendations in the companion informational resource.

Similar pattern of change in V̇o2 kinetics, vascular function, and tissue oxygen provision following an endurance training stimulus in older and young adults.

The purpose of this study was to examine the time course of changes in the oxygen uptake (V̇o2) kinetics response subsequent to short-term exercise training (i.e., 24, 48, 72, and 120 h posttraining) and examine the relationship with the time course of changes in microvascular [deoxygenated hemoglobin concentration ([HHb])-to-V̇o2 ratio ([HHb])/V̇o2)] and macrovascular [flow-mediated dilation (FMD)] O2 delivery to the active tissues/limbs. Seven healthy older [OA; 74 ± 6 (SD) yr] and young men (YA; 25 ± 3 yr) completed three endurance cycling exercise training sessions at 70% V̇o2peak Moderate-intensity exercise on-transient V̇o2 (measured breath by breath) and [HHb] (measured by near-infrared spectroscopy) were modeled with a monoexponential and normalized (0-100% of response), and the [HHb])/V̇o2 was calculated. Ultrasound-derived FMD of the popliteal artery was assessed after 5 min of cuff occlusion. %FMD was calculated as the greatest percent change in diameter from baseline. Time constant of V̇o2 (τV̇o2) was significantly reduced in both OA (~18%) and YA (~23%) at 24 h (P < 0.001) posttraining and remained decreased at 48 h before returning toward pretraining (PRE) values. Both groups showed a significant decrease in the [HHb])/V̇o2 at 24, 48, and 72 h (P = 0.001, 0.01, and 0.03, respectively) posttraining before returning toward PRE values at 120 h. %FMD followed a similar time course to that of changes in the [HHb])/V̇o2, being significantly greater in both OA (by ~64%) and YA (by ~26%) at 24 h (P < 0.001), remaining increased at 48 and 72 h (P = 0.02 and 0.03, respectively), and returning toward PRE values at 120 h. These data suggest the rate of adjustment of V̇o2 may be constrained by O2 availability in the active tissues.

Long-term Evaluation of the "Get Fit for Active Living" Program.

This study examined six- and 12-month levels of adherence to physical activity, functional changes, and psychosocial determinants of physical activity in 176 older adults who participated in the "Get Fit for Active Living (GFAL)" pilot program. Functional and psychosocial measures were conducted in person at six months; psychosocial measures and physical activity participation were assessed by telephone interview at 12 months. Ninety-five per cent were retained in the study at the six-month follow-up, and 88 per cent at 12 months. The self-reported adherence rate to exercise at 12 months was 66 per cent. The main reason for continued exercise participation was to maintain health (45%). Reasons for nonadherence were illness (38%) and lack of motivation (32%). Results identify factors associated with positive behaviour change that health promoters can utilize when targeting the older adult population. The GFAL project results can serve as a model for sustainable, community-based older-adult exercise programs.

Vascular responsiveness measured by tissue oxygen saturation reperfusion slope is sensitive to different occlusion durations and training status.

What is the central question of this study? Is the near-infrared spectroscopy-derived measure of tissue oxygen saturation (StO2) reperfusion slope sensitive to a range of ischaemic conditions, and do differences exist between trained and untrained individuals? What is the main finding and its importance? The StO2 reperfusion rate is sensitive to different occlusion durations, and changes in the reperfusion slope in response to a variety of ischaemic challenges can be used to detect differences between two groups. These data indicate that near-infrared spectroscopy-derived measures of StO2, specifically the reperfusion slope following a vascular occlusion, can be used as a sensitive measure of vascular responsiveness. The reperfusion rate of near-infrared spectroscopy-derived measures of tissue oxygen saturation (StO2) represents vascular responsiveness. This study examined whether the reperfusion slope of StO2 is sensitive to different ischaemic conditions (i.e. a dose-response relationship) and whether differences exist between two groups of different fitness levels. Nine healthy trained (T; age 25 ± 3 years; maximal oxygen uptake 63.4 ± 6.7 ml kg-1  min-1 ) and nine healthy untrained men (UT; age 21 ± 1 years; maximal oxygen uptake 46.6 ± 2.5 ml kg-1  min-1 ) performed a series of vascular occlusion tests of different durations (30 s, 1, 2, 3 and 5 min), each separated by 30 min. The StO2 was measured over the tibialis anterior using near-infrared spectroscopy, with the StO2 reperfusion slope calculated as the upslope during 10 s following cuff release. The reperfusion slope was steeper in T compared with UT at all occlusion durations (P < 0.05). For the T group, the reperfusion slopes for 30 s and 1 min occlusions were less than for all longer durations (P < 0.05). The reperfusion slope following 2 min occlusion was similar to that for 3 min (P > 0.05), but both were less steep than for 5 min of occlusion. In UT, the reperfusion slope at 30 s was smaller than for all longer occlusion durations (P < 0.05), and 1 min occlusion resulted in a reperfusion slope that was less steep than following 2 and 3 min (P < 0.05), albeit not different from 5 min (P > 0.05). The present study demonstrated that the reperfusion rate of StO2 is sensitive to different occlusion durations, and that changes in the reperfusion rate in response to a variety of ischaemic challenges can be used to detect differences in vascular responsiveness between trained and untrained groups.

Repeatability of vascular responsiveness measures derived from near-infrared spectroscopy.

Near-infrared spectroscopy (NIRS)-derived measures of tissue oxygen saturation (StO2) have been recently shown to significantly correlate with the widely used method for noninvasively assessing vascular endothelial function, flow-mediated dilation (FMD). The purpose of this study was to examine the intraday and interday reliability of the reperfusion slope of StO2 (slope 2 StO2) and compare it to FMD Ultrasound-derived FMD was quantified following 5 min of distal cuff occlusion of the popliteal artery in nine healthy young men (26 ± 3 years). An FMD test was performed each of 4 days, with a fifth involving three tests. FMD was calculated as the greatest percent change in diameter from baseline (%FMD). StO2 was measured using NIRS throughout each test, with slope 2 StO2 being calculated as the upslope of 10-sec following cuff release. Reliability was determined using repeatability, intraclass correlation coefficients (ICC), and coefficient of variation (CV). Repeatability of slope 2 StO2 was better than %FMD for both intraday (0.43 and 5.65, respectively) and interday (0.48 and 4.82, respectively) comparisons; approximately 30% of mean values for slope 2 StO2 could be attributed to measurement error, whereas 100% of mean FMD could be for both intraday and interday comparisons. Similarly, ICC and CV values indicated stronger reliability of slope 2 StO2 compared to %FMD for both intraday (ICC 0.92 and 0.36, respectively; CV 9 ± 4% and 44 ± 24%, respectively) and interday (ICC 0.94 and 0.25, respectively; CV 14 ± 5% and 40 ± 22%, respectively) comparisons. In conclusion, NIRS-derived slope 2 StO2 can be used as a reliable measure of vascular reactivity.

Vascular responsiveness determined by near-infrared spectroscopy measures of oxygen saturation.

Vascular impairments at the macro- and microcirculatory levels are associated with increased risk for cardiovascular disease. Flow-mediated dilation (FMD) is currently the most widely used method for non-invasive assessment of vascular endothelial function. Recently, near-infrared spectroscopy (NIRS)-derived measures of tissue oxygen saturation (StO2) have been used to characterize the dynamic response of local tissue perfusion to a brief period of ischaemia. The purpose of the present study was to establish correlations between the reperfusion rate of StO2 and FMD. Ultrasound-derived FMD was quantified after 5 min of distal cuff occlusion of the popliteal artery in 20 healthy young men (26 ± 3 years old). Triplicate measurements of end-diastolic arterial diameter were made every 15 s after cuff release, and FMD response was calculated as the greatest percentage change in diameter from baseline (%FMD). The StO2 was measured using NIRS throughout the duration of each test. Two consecutive FMD tests were performed, separated by 30 min of rest, and were averaged for %FMD and StO2. The %FMD was significantly correlated with the reperfusion slope of StO2 after cuff release (slope 2 StO2; r = 0.63, P = 0.003). In conclusion, the present study established a correlation between slope 2 StO2 and %FMD in healthy young men. These data suggest that NIRS-derived slope 2 StO2 can be used as a measure of vascular endothelial function.

Slower V̇O2 Kinetics in Older Individuals: Is It Inevitable?

INTRODUCTION: The mechanisms controlling the rate of adjustment of oxidative phosphorylation have been debated for several years. Although disagreement exists as to what the prevailing mechanisms controlling the speed of the oxygen uptake (V̇O2) kinetics are in both young and older individuals, it seems tenable that the slower V̇O2 kinetics response typically observed in older adults is at least partly imposed by an O2 delivery limitation. RESULTS: Several studies have demonstrated that different interventions can speed V̇O2 kinetics in older individuals so that this response can become similar to that observed in their young counterparts. These findings have opened the debate as to whether aging per se, or other factors that accompany aging, is responsible for the slower adjustment of oxidative metabolism in the elderly. This review focuses on the slower V̇O2 kinetics often observed in older populations and discusses potential mechanisms that might mediate the slower adjustment in oxidative phosphorylation. Furthermore, interventions that have been successful in speeding V̇O2 kinetics in the elderly are described to discriminate how the controlling factors determining the adjustment of V̇O2 might be regulated by specific perturbations. Importantly, this review shows that the slower adjustment of oxidative phosphorylation typically seen in older compared with young individuals can be completely abolished in some exceptional situations such as chronic endurance-exercise training, despite the age-related decrease in maximal V̇O2 still being present. CONCLUSIONS: Thus, this review focuses on the concept that although V̇O2 kinetics is often slower in the elderly, this slower increase in the rise of oxygen uptake during the exercise on-transient does not need to be considered an inevitable response.

Exercise Intensity Thresholds: Identifying the Boundaries of Sustainable Performance.

UNLABELLED: Critical power (CP), respiratory compensation point (RCP), maximal lactate steady state (MLSS), and deoxyhemoglobin breakpoint ([HHb]BP) are alternative functional indices that are thought to demarcate the highest exercise intensity that can be tolerated for long durations. PURPOSE: We tested the hypothesis that CP, RCP, MLSS, and [HHb]BP occur at the same metabolic intensity by examining the pulmonary oxygen uptake (V˙)O2p and power output (PO) associated with each "threshold." METHODS: Twelve healthy men (mean ± SD age, 27 ± 3 yr) performed the following tests on a cycle ergometer: i) four to five exhaustive tests for determination of CP, ii) two to three 30-min constant-power trials for MLSS determination, and iii) a ramp incremental exercise test from which the V˙O2p and PO at RCP and [HHb]BP were determined. During each trial, breath-by-breath V˙O2p and ventilatory variables were measured with a metabolic cart and flowmeter turbine; near-infrared spectroscopy-derived [HHb] was monitored using a frequency domain multidistance system, and arterialized capillary blood lactate was sampled at regular intervals. RESULTS: There were no differences (P > 0.05) among the V˙O2p values associated with CP, RCP, MLSS, and [HHb]BP (CP, 3.29 ± 0.48; RCP, 3.34 ± 0.45; MLSS, 3.27 ± 0.44; [HHb]BP, 3.41 ± 0.46 L·min(-1)); however, the PO associated with RCP (262 ± 48 W) and [HHb]BP (273 ± 41 W) were greater (P < 0.05) than both CP (226 ± 45 W) and MLSS (223 ± 39 W), which, themselves, were not different (P > 0.05). CONCLUSIONS: Although the standard methods for determination of CP, RCP, MLSS, and [HHb]BP are different, these indices occur at the same V˙O2p, suggesting that i) they may manifest as a result of similar physiological phenomenon and ii) each provides a valid delineation between tolerable and intolerable constant-power exercise.

Effects of age and long-term endurance training on VO2 kinetics.

PURPOSE: This study examined the effects of age and training status on the pulmonary oxygen uptake (VO2p) kinetics of untrained and chronically trained young, middle-age, and older groups of men. METHODS: Breath-by-breath VO2p and near-infrared spectroscopy-derived muscle deoxygenation ([HHb]) were monitored continuously in young (20-39 yr) trained (YT, n = 8) and untrained (YuT, n = 8), middle-age (40-59 yr) trained (MT, n = 9) and untrained (MuT, n = 9), and older (60-85 yr) trained (OT, n = 9) and untrained (OuT, n = 8) men. On-transient VO2p and [HHb] responses to cycling exercise at 80% of the estimated lactate threshold (three repeats) were modeled as monoexponential. Data were scaled to a relative percentage of the response (0%-100%), the signals time aligned, and the individual [HHb]-to-VO2p ratio was calculated as the average [HHb]/VO2 during the 20- to 120-s period after exercise onset. RESULTS: The time constant for the adjustment of phase II pulmonary VO2 (τVO2p) was larger in OuT (42.0 ± 11.3 s) compared with that in YT (17.0 ± 7.5 s), MT (18.1 ± 5.3 s), OT (19.8 ± 5.4 s), YuT (25.7 ± 6.6 s), and MuT (24.4 ± 7.4 s) (P < 0.05). Similarly, the [HHb]/VO2 ratio was larger than 1.0 in OuT (1.30 ± 0.13, P < 0.05) and this value was larger than that observed in YT (1.01 ± 0.07), MT (1.04 ± 0.05), OT (1.04 ± 0.04), YuT (1.05 ± 0.03), and MuT (1.02 ± 0.09) (P < 0.05). CONCLUSIONS: This study showed that the slower VO2kinetics typically observed in older individuals can be prevented by long-term endurance training interventions. Although the role of O2 delivery relative to peripheral use cannot be elucidated from the current measures, the absence of age-related slowing of VO2 kinetics seems to be partly related to a preservation of the matching of O2 delivery to O2 utilization in chronically trained older individuals, as suggested by the reduction in the [HHb]/VO2 ratio.

Pulmonary O2 uptake kinetics during moderate-intensity exercise transitions initiated from low versus elevated metabolic rates: insights from manipulations in cadence.

INTRODUCTION: The rate of adjustment (τ) of phase II pulmonary O2 uptake (VO2p) is slower when exercise transitions are initiated from an elevated baseline work rate (WR) and metabolic rate (MR). In this study, combinations of cycling cadence (40 vs. 90 rpm) and external WR were used to examine the effect of prior MR on τVO2p. METHODS: Eleven young men completed transitions from 20 W (BSL) to 90% lactate threshold, with transitions performed as two steps of equal ∆WR (LS, lower step; US, upper step), while maintaining a cadence of (1) 40 rpm, (2) 90 rpm, and (3) 40 rpm but with the WRs elevated to match the higher VO2p associated with 90 rpm cycling (40MATCH); transitions lasted 6 min. VO2p was measured breath-by-breath using mass spectrometry and turbinometry; vastus lateralis muscle deoxygenation [HHb] was measured using near-infrared spectroscopy. VO2p and HHb responses were modeled using nonlinear least squares regression analysis. RESULTS: VO2p at BSL, LS and US was similar for 90 rpm and 40MATCH, but greater than in 40 rpm. Compared to 90 rpm, τVO2p at 40 rpm was shorter (p < 0.05) in LS (18 ± 5 vs. 28 ± 8 s) but not in US (26 ± 8 vs. 33 ± 9 s), and at 40MATCH, τVO2p was lower (p < 0.05) (19 ± 6 s) in LS but not in US (34 ± 13 s) despite differing external WR and ∆WR. CONCLUSIONS: A similar overall adjustment of [HHb] and VO2p in LS and US across conditions suggested dynamic matching between microvascular blood flow and O2 utilization. Prior MR (rather than external WR per se) plays a role in the dynamic adjustment of pulmonary (and muscle) VO2p.

Breath-by-breath pulmonary O2 uptake kinetics: effect of data processing on confidence in estimating model parameters.

To improve the signal-to-noise ratio of breath-by-breath pulmonary O2 uptake (V̇O2p) data, it is common practice to perform multiple step transitions, which are subsequently processed to yield an ensemble-averaged profile. The effect of different data-processing techniques on phase II V̇O2p kinetic parameter estimates (V̇O2p amplitude, time delay and phase II time constant (τV̇O2p)] and model confidence [95% confidence interval (CI95)] was examined. Young (n = 9) and older men (n = 9) performed four step transitions from a 20 W baseline to a work rate corresponding to 90% of their estimated lactate threshold on a cycle ergometer. Breath-by-breath V̇O2p was measured using mass spectrometry and volume turbine. Mono-exponential kinetic modelling of phase II V̇O2p data was performed on data processed using the following techniques: (A) raw data (trials time aligned, breaths of all trials combined and sorted in time); (B) raw data plus interpolation (trials time aligned, combined, sorted and linearly interpolated to second by second); (C) raw data plus interpolation plus 5 s bin averaged; (D) individual trial interpolation plus ensemble averaged [trials time aligned, linearly interpolated to second by second (technique 1; points joined by straight-line segments), ensemble averaged]; (E) 'D' plus 5 s bin averaged; (F) individual trial interpolation plus ensemble averaged [trials time aligned, linearly interpolated to second by second (technique 2; points copied until subsequent point appears), ensemble averaged]; and (G) 'F' plus 5 s bin averaged. All of the model parameters were unaffected by data-processing technique; however, the CI95 for τV̇O2p in condition 'D' (4 s) was lower (P < 0.05) than the CI95 reported for all other conditions (5-10 s). Data-processing technique had no effect on parameter estimates of the phase II V̇O2p response. However, the narrowest interval for CI95 occurred when individual trials were linearly interpolated and ensemble averaged.

Faster VO(2) kinetics after eccentric contractions is explained by better matching of O(2) delivery to O(2) utilization.

PURPOSE: This study examined the impact of eccentric exercise-induced muscle damage on the rate of adjustment in muscle deoxygenation and pulmonary O2 uptake (VO(2p)) kinetics during moderate exercise. METHODS: Fourteen males (25 ± 3 year; mean ± SD) completed three step transitions to 90 % θL before (Pre), 24 h (Post24) and 48 h after (Post48) eccentric exercise (100 eccentric leg-press repetitions with a load corresponding to 110 % of the participant's concentric 1RM). Participants were separated into two groups: phase II VO(2p) time constant (τVO(2p)) ≤ 25 s (fast group; n = 7) or τVO(2p) > 25 s (slow group; n = 7). VO(2p) and [HHb] responses were modeled as a mono-exponential. RESULTS: In both groups, isometric peak torque (0°/s) at Post24 was decreased compared to Pre (p < 0.05) and remained depressed at Post48 (p < 0.05). τVO(2p) was designed to be different (p < 0.05) at Pre between the Fast (τVO(2p); 19 ± 4 s) and Slow (32 ± 6 s) groups. There were no differences among time points (τVO(2p): Pre, 19 ± 4 s; Post24, 22 ± 3 s; Post48, 20 ± 4 s) in the Fast group. In Slow, there was a speeding (p < 0.05) from the Pre (32 ± 6 s) to the Post24 (25 ± 6) but not Post48 (31 ± 6), resulting in no difference (p > 0.05) between groups at Post24. This reduction of τVO(2p) was concomitant with the abolishment (p < 0.05) of an overshoot in the [HHb]/VO(2p) ratio. CONCLUSION: We propose that the sped VO(2p) kinetics observed in the Slow group coupled with an improved [HHb]/VO(2p) ratio suggest a better matching of local muscle O2 delivery to O2 utilization following eccentric contractions.