Continuous-Flow High-Pressure Homogenization of Blueberry Juice Enhances Anthocyanin and Ascorbic Acid Stability during Cold Storage.

Blueberries (Vaccinium section Cyanococcus) have a wealth of bioactive compounds, including anthocyanins and other antioxidants, that offer significant health benefits. Preserving these compounds and maintaining the sensory and nutritional qualities of blueberry products such as juice during cold market storage is critical to meet consumer expectations for nutritious, safe, and minimally processed food. In this study, we compared the effects of two preservation processing techniques, high-temperature short-time (HTST) and continuous flow high-pressure homogenization (CFHPH), on blueberry juice quality during storage at 4 °C. Our findings revealed that inlet temperature (Tin) of CFHPH processing at 4 °C favored anthocyanin retention, whereas Tin at 22 °C favored ascorbic acid retention. After 45 days of storage, CFHPH (300 MPa, 1.5 L/min, 4 °C) juice retained up to 54% more anthocyanins compared to control at 0 day. In contrast, HTST treatment (95 °C, 15 s) initially increased anthocyanin concentrations but led to their subsequent degradation over time, while also significantly degrading ascorbic acid. Furthermore, CFHPH (300 MPa, 4 °C) juice had significantly lower polyphenol oxidase activity (>80% less than control), contributing to the overall quality of the juice. This innovative processing technique has the potential to improve commercial blueberry juice, and help meet the rising demand for healthy and appealing food choices.

Bacterial diversity and composition on the rinds of specific melon cultivars and hybrids from across different growing regions in the United States.

The goal of this study was to characterize the bacterial diversity on different melon varieties grown in different regions of the US, and determine the influence that region, rind netting, and variety of melon has on the composition of the melon microbiome. Assessing the bacterial diversity of the microbiome on the melon rind can identify antagonistic and protagonistic bacteria for foodborne pathogens and spoilage organisms to improve melon safety, prolong shelf-life, and/or improve overall plant health. Bacterial community composition of melons (n = 603) grown in seven locations over a four-year period were used for 16S rRNA gene amplicon sequencing and analysis to identify bacterial diversity and constituents. Statistically significant differences in alpha diversity based on the rind netting and growing region (p < 0.01) were found among the melon samples. Principal Coordinate Analysis based on the Bray-Curtis dissimilarity distance matrix found that the melon bacterial communities clustered more by region rather than melon variety (R2 value: 0.09 & R2 value: 0.02 respectively). Taxonomic profiling among the growing regions found Enterobacteriaceae, Bacillaceae, Microbacteriaceae, and Pseudomonadaceae present on the different melon rinds at an abundance of ≥ 0.1%, but no specific core microbiome was found for netted melons. However, a core of Pseudomonadaceae, Bacillaceae, and Exiguobacteraceae were found for non-netted melons. The results of this study indicate that bacterial diversity is driven more by the region that the melons were grown in compared to rind netting or melon type. Establishing the foundation for regional differences could improve melon safety, shelf-life, and quality as well as the consumers' health.

The expression and function of growth hormone secretagogue receptor in immune cells: A current perspective.

The orexigenic hormone ghrelin and its receptor, growth hormone secretagogue receptor (GHS-R), have been extensively studied in the last two decades, revealing that ghrelin signaling has important implications in health and disease. Metabolic diseases, such as obesity and diabetes, are often accompanied by low-grade chronic inflammation, that has been coined as "meta-inflammation." Immune cells are key cellular mediators of meta-inflammation, controlling both initiation and resolution of inflammation. Immune cells exhibit dynamic changes in cellular characteristics and functional output in response to the stimuli/insults from their surrounding microenvironment. Emerging evidence shows that ghrelin has an important effect on inflammation, in addition to its well-known effects on metabolism. However, the cellular/molecular mechanism of ghrelin signaling in immunity is largely unknown because the knowledge in regard to the expression and function of GHS-R in immune cells is currently sparse. In this review, we have accumulated the recent findings related to the expression and functions of GHS-R in various immune cells under different physiological and pathological states. This review aims to inspire further investigation of the immunological roles of ghrelin signaling and advance the therapeutic applications of ghrelin signaling in meta-inflammation.

Raman Spectroscopy Detects Changes in Carotenoids on the Surface of Watermelon Fruits During Maturation.

A non-invasive and non-destructive technique, Raman spectroscopy, was explored to distinguish different maturity stages (20, 30, 40, and 50 days after anthesis) of watermelon (Citrullus lanatus) fruits from four cultivars: Fascination, Orange Crisp, Amarillo and Crimson Sweet. Spectral acquisition from the fruit surface was carried out at the wavelength range of 400-2,000 cm-1 using a handheld Raman spectrometer equipped with 830 nm laser excitation source. The spectra were normalized at 1,438 cm-1 which was assigned to CH2 and CH3 vibration. Detecting changes in the spectral features of carotenoids on the surface of watermelon fruits can be used as a marker to monitor the maturity of the fruit. The spectral analysis confirmed the presence of two major carotenoids, lutein and ß-carotene, and their intensity decreased upon maturity on the fruit surface. Identification of these pigments was further confirmed by resonance Raman spectra and high-performance liquid chromatography analysis. Results of partial least square discriminant analysis of pre-processed spectra have demonstrated that the method can successfully predict the maturity of watermelon samples with more than 85% accuracy. Analysis of Variance of individual Raman bands has revealed a significant difference among the stages as the level of carotenoids was declined during the ripening of the fruits. Thus, Raman spectral signatures can be used as a versatile tool for the non-invasive determination of carotenoid changes on the watermelon fruits' surface during ripening, thereby enabling effective monitoring of nutritional quality and maturity indices before harvesting the watermelon.

Separation of nordihydrocapsiate from capsiate and major capsaicinoid analogues using ultra high performance liquid chromatography.

Accurate, rapid quantitation of the capsaicinoid and capsinoid compounds produced by peppers (Capsicum spp.) is essential to assess quality. Here, we developed a rapid ultra-high performance liquid chromatography method for the simultaneous separation of five major capsaicinoids and three major capsinoids from peppers. Optimal chromatographic separation was achieved using a phenyl-hexyl stationary phase with a mobile phase of acidified water and methanol with a flow rate of 0.5 ml/min at a column temperature of 55 °C over 5 min. The method was validated by testing linearity, precision, robustness, and limits of detection and quantification. The developed method was successfully employed to profile capsaicinoids and capsinoids from different pepper cultivars. Out of the 10 pepper cultivars analysed, all three major capsinoids were detected in two cultivars. To the best of our knowledge, this is the first report of successful separation of nordihydrocapsiate from capsiate and quantification of nordihydrocapsiate.

Assessment and Classification of Volatile Profiles in Melon Breeding Lines Using Headspace Solid-Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry.

Cucumis melo L is one of the most commercial and economical crops in the world with several health beneficial compounds as such carotenoids, amino acids, vitamin A and C, minerals, and dietary fiber. Evaluation of the volatile organic compounds (VOCs) in different melon (Cucumis melo L.) breeding lines provides useful information for improving fruit flavor, aroma, and antimicrobial levels. In this study, the VOCs in 28 melon breeding lines harvested in 2019 were identified and characterized using head space solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This identified 113 VOCs with significant differences in composition and contents of among the breeding lines, including 15 esters, 27 aldehydes, 35 alcohols, 14 ketones, 4 acids, 10 hydrocarbons, 5 sulfurs, and 3 other compounds. The highest average contents of all the VOCs were found in BL-30 (13,973.07 µg/kg FW) and the lowest were in BL-22 (3947.13 µg/kg FW). BL-9 had high levels of carotenoid-derived VOCs. The compounds with the highest contents were benzaldehyde, geranylacetone, and ß-ionone. Quality parameters such as color and sugar contents of melons were also measured. All the melon color readings were within the typical acceptable range. BL-22 and BL-14 had the highest and lowest sugar contents, respectively. Principal component analysis (PCA) produced diverse clusters of breeding lines based on flavor and aroma. BL-4, BL-7, BL-12, BL-20, and BL-30 were thus selected as important breeding lines based on their organoleptic, antimicrobial, and health-beneficial properties.

Nutritional Composition and Health Benefits of Various Botanical Types of Melon (Cucumis melo L.).

Characterizing the diverse melon cultivars for nutrition aids in crop improvement and promoting a healthy diet. Here, we used in vitro assays to characterize the nutritional qualities and health-beneficial effects of 30 melon (Cucumis melo L.) genotypes, including 10 improved cultivars, 16 landraces, and 4 wild types collected from different parts of India. Two landraces (Sidoota and Alper Green) had the highest (12.20 and 11.25) total soluble solids (TSS) contents. The Sidoota and Pappusa landraces had high reducing sugar contents (2.84 and 2.81 mg g-1 fresh weight [FW]). The highest polyphenols contents (22.0 mg g-1 FW) were observed in the landraces Mage Kaayi-2, Budamekaayi, and Small Melon. Reflecting on the primary and secondary metabolite contents, the Mekke Kaayi and Giriyala landraces exhibited high 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (97.6 and 91% at 100 µg mL-1). Additionally, seven of the landraces showed significant nitric oxide (NO) induction activity (>80% inhibition at 200 µg mL-1), indicating their potential health benefits, and seven showed considerable angiotensin-converting enzyme (ACE) inhibition activity (highest in Kashi Madhu), indicating their potential usefulness in reducing hypertension. Genotypes with high health beneficial compounds identified in this study can be used for breeding improved melon cultivars to promote these fruits as well as a healthy diet.

Optimization of Extraction Solvent and Fast Blue BB Assay for Comparative Analysis of Antioxidant Phenolics from Cucumis melo L.

Melon (Cucumis melo L.) fruits contain multiple health-promoting compounds, including phenolic compounds, which are antioxidants. Accurate measurement of antioxidant activities and total phenolic contents (TPCs) require an efficient solvent extraction. In this study, we evaluated free radical scavenging activity and TPC of melon extracts extracted with 22 different solvent combinations. The DPPH scavenging activities were high in 100% methanolic (39.48 ± 0.36 µg g-1) and 80% methanolic extracts (38.99 ± 0.44 µg g-1). Similarly, the ABTS scavenging activities were high in 100% methanolic (315.11 ± 10.38 µg g-1) and 80% methanol extracts (297.39 ± 14.98 µg g-1). The Folin-Ciocalteu (F-C) assay is typically used to measure TPC but may be affected by interference from sugars and other compounds. Therefore, we optimized an assay for TPC using Fast Blue (FB) salt and developed a standard operating procedure for microplate analysis using FB. Our analysis of standard samples and comparisons with the F-C assay suggested that the optimized FB assay could be used to measure TPC in fruit and juice samples. Moreover, we successfully detected six phenolic compounds in methanol extracts of melon by LC-HR-QTOF/MS.

Manganese Oxide Nanoparticles as Safer Seed Priming Agent to Improve Chlorophyll and Antioxidant Profiles in Watermelon Seedlings.

The use of nanoscale nutrients in agriculture to improve crop productivity has grown in recent years. However, the bioefficacy, safety, and environmental toxicity of nanoparticles are not fully understood. Herein, we used onion bulb extract to synthesize manganese oxide nanoparticles (MnO-NPs). X-ray diffraction, X-ray photoelectron spectroscopy, and high-resolution transmission electron microscopy were used for the structural and morphological characterization of synthesized MnO-NPs. The MnO-NPs were oval shape crystalline nanoparticles of Mn2O3 with sizes 22-39 nm. In further studies, we assessed the comparative toxicity of seed priming with MnO-NPs and its bulk counterparts (KMnO4 and Mn2O3), which showed seed priming with MnO-NPs had comparatively less phytotoxicity. Investigating the effect of seed priming with different concentrations of MnO-NPs on the hormonal, phenolic acid, chlorophyll, and antioxidant profiles of watermelon seedlings showed that treatment with 20 mg·L-1 MnO-NPs altered the chlorophyll and antioxidant profiles of seedlings. At ≤40 mg·L-1, MnO-NPs had a remarkable effect on the phenolic acid and phytohormone profiles of the watermelon seedlings. The physiological outcomes of the MnO-NP seed priming in watermelon were genotype-specific and concentration-dependent. In conclusion, the MnO-NPs were safer than their bulk counterparts and could increase crop productivity.

Production system influences tomato phenolics and indoleamines in a cultivar-specific manner.

Tomato (Solanum lycopersicum) fruit is a rich source of health-promoting compounds, and epidemiological studies show that tomato consumption may reduce the risk of chronic diseases. This study compared the effect of genotype, production system, and their interaction on eight tomato varieties grown in the open-field (OF) or net-house (NH), a structure completely covered with a 50-mesh screen to reduce pest and wind damage, in South Texas. The NH structure reduced solar radiation up to ~30% and decreased wind speed by 6.44 km/h compared with conditions measured in the OF. We simultaneously analyzed 16 phenolics and indoleamines using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight high-resolution mass spectrometry (UHPLC/ESI-HR-QTOFMS). The chemometric analysis showed a distinct difference between NH- and OF-grown tomatoes irrespective of the variety. The melatonin and serotonin contents showed a cultivar-specific effect of the production system. Likewise, the effect of cultivation systems on levels of phenolic acids and flavonoids varied based on tomato cultivar. Among the studied phenolic acids, significantly enhanced levels of sinapic acid were observed in OF-grown tomatoes. Similarly, we detected a considerable genotypic effect on gallic acid, p-coumaric acid, ferulic acid, sinapic acid, and naringin. The interaction of cultivar and production system substantially affected gallic acid, protocatechuic acid, sinapic acid, and apigenin. However, further studies need to be performed to explore the environment-specific effects on the total composition. In summary, our results indicate that the production system plays an important role in tomato composition beyond the natural genetic variation among cultivars.

Citrus limonoids induce apoptosis and inhibit the proliferation of pancreatic cancer cells.

In our recent study, we demonstrated that certain limonoids isolated from citrus seeds induced apoptosis in human pancreatic (Panc-28) cells. In this study, limonin, nomilin and limonexic acid (LNA) were investigated for understanding the possible mode of cytotoxicity in cultured pancreatic cancer (Panc-28) cells. All three limonoids inhibited Panc-28 cell proliferation, with IC50 values less than 50 µM after 72 h of incubation. The induction of apoptosis was confirmed through the cleavage of caspase-3, decreased mitochondrial membrane potential, and expression of apoptosis-related proteins. The Bax/Bcl2 expression ratio was increased up to 11-fold in cells pre-treated with 60 µM limonoids for 48 h. Apart from this, the limonoids also induced the expression of p21, and exhibited anti-inflammatory activity through decreasing the expression of cox-2, NF-κB and IL-6. Based on these results, we were interested in understanding the possible mode of inhibition by LNA, which exhibited the highest activity. The treatment of Panc-28 cells resulted in dose- and time-dependent induction of apoptosis-inducible proteins. In addition, treatment with 60 µM LNA resulted in the activation of Akt-associated signals to induce apoptosis, and the same was confirmed by the effects of the compounds on pAkt, p53, VEGF and caspase proteins. The results of this study demonstrated the cytotoxicity of limonoids to human pancreatic cancer cells through the modulation of genes involved in proliferation and survival.

Improved Sample Preparation and Optimized Solvent Extraction for Quantitation of Carotenoids.

Accurate, rapid quantitation of key antioxidants such as carotenoids is important for assessment of food quality. Carotenoids are lipid-soluble pigments that are susceptible to oxidation due to their highly conjugated carbon-carbon double bonds. Therefore, the present work focuses on improving sample preparation to facilitate rapid analysis of carotenoids. The method involves optimized carotenoid extraction followed by direct HPLC analysis without further concentration and redissolution. For extraction, we tested the effect of blending time (1, 3 and 5 min) and 12 different solvent combinations for carotenoid extraction from cantaloupe (Cucumis melo var. cantalupensis) and oranges (Citrus sinensis), two popular fruits that are high in carotenoids. The identification of carotenoids was performed by LC-APCI-QTOF-HR-MS in positive-ionization mode. In melon, 1 min blending time gave significantly higher ß-carotene content with CHCl3: Ace (1:1) solvent. The optimized method was validated with tomato, watermelon, oranges, grapefruit, melon varieties and commercial products such as fruit juices. Among the different melon varieties, Western Shipper had significantly higher ß-carotene (25.1 ± 0.4 µg/g) contents. In oranges, ß-carotene and (all-E)-lycopene contents were 4.4 ± 0.1and 3.8 ± 0.1 µg/g, respectively. The optimized method has fewer unit operations and is reproducible for the quantitation of carotenoids and their isomers. This is the first report on the identification of ζ-carotene isomers, and lycopene isomers from cantaloupe varieties and lycopene from oranges. Graphical Abstract.

Effect of production system and inhibitory potential of aroma volatiles on polyphenol oxidase and peroxidase activity in tomatoes.

BACKGROUND: Polyphenol oxidase (PPO) and peroxidase (POD) are key enzymes associated with shelf life and defense responses. Thus, the activity of PPO and POD enzymes is usually assessed to check the quality of food samples and to understand the physiological responses of plants to different stresses. However, the outcomes of PPO and POD activity assessment studies are highly dependent on assay conditions. Hence, in this study, we initially optimized PPO and POD extraction and high-throughput 96-well plates-based enzymatic activity assessment methods to evaluate the inhibitory potential of tomato volatile compounds. Later, we explored the effects of net-house and open-field growing on the PPO and POD activity in tomato fruits of eight cultivars. RESULTS: We found 150 mM of catechol and pH 7.0 were the optimal conditions for the maximum activity for the PPO assay. Conversely, 24 mM guaiacol with 12 mM H2 O2 and pH 6.0 was the best condition for the POD assay. Thermal inactivation studies confirmed that tomato POD is more resistant to heat than PPO. We found that the production systems had a considerable genotype-specific impact on tomato PPO and POD activity. Moreover, amongst the volatiles that were studied, ß-damascenone and d-limonene showed 50% PPO inhibition at 40 and 80 mM, respectively. CONCLUSION: The results of this study can be used to improve the shelf-life of fresh tomato fruit and its products. The findings also underscore the significance of PPO and POD enzymes as physiological trait markers in the tomato crop and fruit quality improvement programs. © 2020 Society of Chemical Industry.

Effects of genotype and production system on quality of tomato fruits and in vitro bile acids binding capacity.

Tomato is an important source of health-promoting constituents, and researchers have focused on enhancing the content. In the present study, the influence of net-house (NH) and open-field (OF) growing conditions on physicochemical traits of tomatoes from eight different cultivars were evaluated. The tomato fruit qualities, such as color, total soluble solids (TSS), total acidity (TA), and pH were measured. Furthermore, ultra-performance liquid chromatography coupled to electrospray ionization high-resolution quadrupole time-of-flight mass spectrometry (UPLC/ESI-HR-QTOFMS) was used for identification and quantification of health-promoting compounds such as ascorbic acid, ß-carotene, lycopene, and its isomers. In addition, in vitro bile acid binding capacity of all tomato samples was analyzed along with soluble and insoluble dietary fiber analysis as biofunctional properties. The results suggest that production systems influenced tomato fruit quality and biofunctional characteristics in a variety-specific manner. Notably, TA and all-trans-ß-carotene values were considerably influenced by production systems, and their levels were higher in the NH- and OF-grown tomatoes, respectively. Our findings underline the importance of the rational choice of genotype and production system to obtain high-quality tomatoes with enhanced desired traits for breeders and consumers. PRACTICAL APPLICATION: Tomato is one of the nutritional high-valued horticultural crops. The present study aimed to assess the impact of production systems, such as net-house and open-field conditions, on physicochemical traits and biofunctional properties, in vitro bile acid binding capacity of eight tomato varieties. This study supplies a good reference for the rational selection of genotype and production system to obtain high-quality tomatoes with improved desired traits for breeders and consumers.

Hormonal and metabolites responses in Fusarium wilt-susceptible and -resistant watermelon plants during plant-pathogen interactions.

BACKGROUND: Fusarium oxysporum f. sp. niveum (FON) causes Fusarium wilt in watermelon. Several disease-resistant watermelon varieties have been developed to combat Fusarium wilt. However, the key metabolites that mount defense responses in these watermelon varieties are unknown. Herein, we analyzed hormones, melatonin, phenolic acids, and amino acid profiles in the leaf tissue of FON zero (0)-resistant (PI-296341, Calhoun Grey, and Charleston Grey) and -susceptible (Sugar Baby) watermelon varieties before and after infection. RESULTS: We found that jasmonic acid-isoleucine (JA-Ile) and methyl jasmonate (MeJA) were selectively accumulated in one or more studied resistant varieties upon infection. However, indole-3-acetic acid (IAA) was only observed in the FON 0 inoculated plants of all varieties on the 16th day of post-inoculation. The melatonin content of PI-296341 decreased upon infection. Conversely, melatonin was only detected in the FON 0 inoculated plants of Sugar Baby and Charleston Grey varieties. On the 16th day of post-inoculation, the lysine content in resistant varieties was significantly reduced, whereas it was found to be elevated in the susceptible variety. CONCLUSIONS: Taken together, Me-JA, JA-Ile, melatonin, and lysine may have crucial roles in developing defense responses against the FON 0 pathogen, and IAA can be a biomarker of FON 0 infection in watermelon plants.

Multivariate Analysis of Amino Acids and Health Beneficial Properties of Cantaloupe Varieties Grown in Six Locations in the United States.

Cantaloupe is a good dietary source of amino acids, including γ-aminobutyric acid (GABA), glutamine, and citrulline. However, the levels of these amino acids vary among different cantaloupe varieties grown in different locations. Understanding the variation in amino acid contents provides fundamentally important information for quality control and improving melon varieties. To examine this variation, we measured the amino acid contents in cantaloupes grown in six locations in the United States (Texas, Georgia, North Carolina, California, Indiana, and Arizona). Principal component analyses were applied to analyze the effect of growing location on the amino acid profiles in different varieties. The GABA content ranged from 1006.14 ± 64.77 to 3187.12 ± 64.96 µg/g and citrulline ranged from 92.65 ± 9.52 to 464.75 ± 34.97 µg/g depending on the variety and location. Total phenolic contents, α-amylase inhibition, and antioxidant activities were also measured. Tuscan type Da Vinci had significantly higher phenolic contents in Arizona (381.99 ± 16.21 µg/g) but had the lowest level when grown in California (224.56 ± 14.62 µg/g). Our analyses showed significant differences in amino acid levels, phenolics contents, and antioxidant activity in the cantaloupe varieties based on the growing location. These findings underline the importance of considering growing location in the selection and improvement of cantaloupe varieties.

1H Nuclear Magnetic Resonance and Liquid Chromatography Coupled with Mass Spectrometry-Based Metabolomics Reveal Enhancement of Growth-Promoting Metabolites in Onion Seedlings Treated with Green-Synthesized Nanomaterials.

Seed priming is a promising approach to improve germination, emergence, and seedling growth by triggering pre-germinative metabolism and enhancing seedling vigor. Recently, nanopriming gained importance in seed improvement as a result of the small size and unique physicochemical characteristics of nanomaterials. In the present study, silver and gold nanoparticles were synthesized using onion extracts as the reducing agent. Similarly, the agro-food industrial byproducts citrus seed oil and curcumin-removed turmeric oleoresin were used for the preparation of nanoemulsions. For seed priming, these green-synthesized nanomaterials were incubated with seeds of two onion (Allium cepa L.) cultivars (Legend and 50147) for 72 h, and then the plants were grown in a greenhouse for 3 weeks. Seed priming with these nanomaterials increased seed germination and seedling emergence. One-dimensional 1H nuclear magnetic resonance and liquid chromatography coupled with mass spectrometry metabolomics studies showed that different nanopriming treatments distinctly altered the metabolome of onion seedlings. Seed priming treatments significantly inhibited plant hormones and growth regulators, such as abscisic acid and cis-(+)-12-oxo-phytodienoic acid, and enhanced germination stimulators, such as γ-aminobutyric acid and zeatin, in onion seeds and seedlings. Therefore, these priming treatments have positive impact on improving seed performance and plant growth.

Nanoparticle-Mediated Seed Priming Improves Germination, Growth, Yield, and Quality of Watermelons (Citrullus lanatus) at multi-locations in Texas.

Seed priming uses treatments to improve seed germination and thus potentially increase growth and yield. Low-cost, environmentally friendly, effective seed treatment remain to be optimized and tested for high-value specialty crop like watermelon (Citrullus lanatus) in multi-locations. This remains a particularly acute problem for triploids, which produce desirable seedless watermelons, but show low germination rates. In the present study, turmeric oil nanoemulsions (TNE) and silver nanoparticles (AgNPs) synthesized from agro-industrial byproducts were used as nanopriming agents for diploid (Riverside) and triploid (Maxima) watermelon seeds. Internalization of nanomaterials was confirmed by neutron activation analysis, transmission electron microscopy, and gas chromatography-mass spectrometry. The seedling emergence rate at 14 days after sowing was significantly higher in AgNP-treated triploid seeds compared to other treatments. Soluble sugar (glucose and fructose) contents were enhanced during germination in the AgNP-treated seeds at 96 h. Seedlings grown in the greenhouse were transplanted at four locations in Texas: Edinburg, Pecos, Grapeland, and Snook in 2017. At Snook, higher yield 31.6% and 35.6% compared to control were observed in AgNP-treated Riverside and Maxima watermelons, respectively. To validate the first-year results, treated and untreated seeds of both cultivars were sown in Weslaco, Texas in 2018. While seed emegence and stand establishments were enhanced by seed priming, total phenolics radical-scavenging activities, and macro- and microelements in the watermelon fruits were not significantly different from the control. The results of the present study demonstracted that seed priming with AgNPs can enhance seed germination, growth, and yield while maintaining fruit quality through an eco-friendly and sustainable nanotechnological approach.

UPLC-QTOF-MS fingerprinting combined with chemometrics to assess the solvent extraction efficiency, phytochemical variation, and antioxidant activities of Beta vulgaris L.

Accurate assays of plant antioxidants and other phytochemicals require efficient extraction conditions and enable rigorous assessments of crop varieties and production systems. This study assessed the extraction of phytochemicals and antioxidants from conventionally or organically grown red and golden beets (Beta vulgaris L.), using twenty solvent (S1-S20) mixtures containing water, methanol, and ethanol alone or with acids (ascorbic, formic, acetic). Red beetroot extracted with methanol with or without acid had the highest betanin content (2791.0 µg/g and 8222.3 µg/g of fresh weight [FW], respectively) and golden beetroot extracted with methanol/ascorbic acid/water had the highest vulgaxanthin I (193.7 µg/g and 15.0 µg/g of FW, respectively). The radical-scavenging activity and total phenolics in beetroot extracts reflected the different extraction efficiency of each solvent. UHPLC-QTOF-MS was used to identify twenty-seven phytochemicals, including 23 betalains, 2 amino acids, and 2 phenolic acids. Chemometric approaches discriminated the beet varieties and different extracts within one variety based on the composition and abundance of the key phytochemicals. The red beetroot extracted with aqueous ethanol with or without acid (S5, S7, S8, S9), and golden beetroot extracted with methanol-containing solvents (S15 for conventionally and S20 for organically) had the highest levels of phytochemicals, suggesting that these conditions efficiently extract key phytochemicals.

CIRCADIAN CLOCK-ASSOCIATED1 Controls Resistance to Aphids by Altering Indole Glucosinolate Production.

CIRCADIAN CLOCK-ASSOCIATED1 (CCA1), a well-known central circadian clock regulator, coordinates plant responses to environmental challenges. Its daily rhythmic expression in Arabidopsis (Arabidopsis thaliana) confers host resistance to the caterpillar Trichoplusia ni However, it is unclear whether CCA1 plays a role in defense against phloem sap-feeding aphids. In this study, we showed that green peach aphid (Myzus persicae) displayed an intrinsic circadian feeding rhythm. Under constant light, wild-type Columbia-0 (Col-0) Arabidopsis plants coentrained with aphids in the same light/dark cycles exhibited greater antixenotic activity than plants preentrained in the opposite cycle from the aphids. Consistently, circadian mutants cca1-1, cca1-11, lhy-21, ztl-1, ztl-4, and lux-2 suffered more severe damage than Col-0 plants when infested by aphids, suggesting that the Arabidopsis circadian clock plays a defensive role. However, the arrhythmic CCA1 overexpression line (CCA1-OX) displayed strong antixenotic and antibiotic activities despite its loss of circadian regulation. Aphids feeding on CCA1-OX plants exhibited lower reproduction and smaller body size and weight than those on Col-0. Apparently, CCA1 regulates both clock-dependent and -independent defense responses. Systematic investigation based on bioinformatics analyses indicated that resistance to aphids in CCA1-OX plants was due primarily to heightened basal indole glucosinolate levels. Interestingly, aphid feeding induced alternatively spliced intron-retaining CCA1a/b transcripts, which are normally expressed at low levels, whereas expression of the major fully spliced CCA1 transcript remained largely unchanged. We hypothesize that posttranscriptional modulation of CCA1 expression upon aphid infestation maximizes the potential of circadian-mediated defense and stress tolerance while ensuring normal plant development.