Antimicrobial flavonoid: in silico targeting Escherichia coli DNA gyrase adeptly.

A phytochemical investigation of Leucas aspera involved the isolation of flavones and steroids, along with triterpenoids. The identified chemical constituents were characterized as baicalein, baicalein-7-O-ß-D-glucuronide, lup-20(29)-ene-3α,23-diol, ß-sitosterol, and stigmasterol. Notably, baicalein (4) and baicalein-7-O-ß-D-glucuronide (5) demonstrated significant antimicrobial activity at a low minimum inhibitory concentration against Staphylococcus aureus, Pseudomonas fluorescens, and Escherichia coli. Meanwhile, lup-20(29)-ene-3α,23-diol, ß-sitosterol, and stigmasterol displayed moderate antimicrobial activity against all the screened microorganisms. Further analysis showed that baicalein-7-O-ß-D-glucuronide had a strong docking score of -5.78 kcal/mol, indicating its robust interaction with E. coli DNA gyrase, which aligns with the in vitro findings. Additionally, baicalein-7-O-ß-D-glucuronide exhibited in silico ADME (absorption, distribution, metabolites, and excretion) properties similar to those of tetracycline.

GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod.

Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-mass spectrometry (LC/MS) analysis. GC/MS analysis revealed twenty compounds in the extract, while LC/MS analysis identified twenty-four compounds. GC/MS chromatogram analysis suggested the presence of opioid α-N-Normethadol a major constituent found in methanolic extract and fatty acid esters carotenoid is found second major constituent. LC/MS chromatogram and the mass spectral analysis demonstrated the presence of flavonoids, carotenoids, and alkaloids as major phytochemicals. We investigated the antibacterial, anti-fungal, and anti-oxidant activity of pod methanolic extract. The extract was found equally effective against E. coli, S. pyogenes, and P. aeruginosa with MIC 100 µg/mL similar to the standard Ampicillin (MIC 100 µg/mL). C. albicans were found to be most susceptible to Vign unguiculata pods methanolic extract with a MIC of 250 µg/mL. The pod extract showed significant DPPH scavenging activity (IC50 =78.38±0.15) which suggests its antioxidant potential.

Functionalized non-woven surfaces for combating the spread of the COVID-19 pandemic.

The worldwide outbreak of SARS-CoV-2 infection has necessitated mandatory use of face masks, personal protective equipment and intake of a healthy diet for immunity boosting. As per WHO's recommendation, continuous use of masks has been proven effective in decreasing the SARS-CoV-2 infection rate. The present study reports on the bacterial filtration efficacy (BFE) of a novel 4-ply functionalized non-woven face mask. We synthesized a polypropylene-based fabric with inner layers of melt-blown fine fibres coated with polylactic acid and immune-boosting herbal phytochemicals. Experimental studies on the synthesized face mask demonstrated a BFE of greater than 99% against Staphylococcus aureus (a bacterium species frequently found in mammalian respiratory tract). A thorough computational analysis using LibDock algorithm demonstrated an effective docking performance of herbal phytochemicals against harmful virus structures. More importantly, the face mask also showed sufficient and stable breathability as per regulatory standards. A breathing resistance of 30 Pa at an aerosol flow rate of 30 l h-1 was reported under standard temperature and pressure conditions, indicating a high potential for real-world applications.

Prevalence and subtypes of hypertension in normal-weight and obese Indian adolescents: a cross-sectional study.

This cross-sectional study examined the prevalence, stages, subtypes of hypertension, and the associated risk factors in adolescent school children in Western India. We screened 2,644 adolescents, from 10 different private and government schools in urban and rural areas for hypertension, as defined by the 2017 Clinical Practice Guidelines. The association of stages and subtypes with age, gender, body mass index, type of school, and place of residence was analysed. 197 children (7.5%) had hypertension; 170 (6.4%) had stage I, 27 (1%) had stage II and 76 (2.9%) had elevated blood pressure (EBP). The risk of EBP was higher in children > 15 years of age (p = 0.006). Compared with normal-weight children, obese, and overweight children had a higher risk of hypertension [odds ratio (OR) 9 (5.84, 13.88) and 3.77 (2.59, 5.48) respectively], whereas underweight children had a lower risk [OR 0.39 (0.16, 0.98)]. Normal-weight hypertension was seen in 5.2% and was higher in children from government schools (9.4%). Systolic-diastolic hypertension (SDH) was the most common subtype, seen in 136 (5.1%). SDH was more common in girls, in rural children, and in those with stage II hypertension. Isolated diastolic hypertension, seen in 51 (1.9%), was more common in boys, in urban children, and in those with EBP.

Phytochemical analysis by GC-MS, LC-MS complementary approaches and antimicrobial activity investigation of Vigna unguiculata (L.) Walp. leaves.

Consumption of legumes has long been linked to their nutritional and medicinal benefits. Vigna unguiculata (L.) Walp. (Cowpea) is a legume plant in the Fabaceae family and is a rich source of nutrients also is known for its beneficial effects for diseases treatment. In terms of phytochemicals analysis and bioactivities evaluations the major research has focused on the Cowpea seeds, whereas leaves and pods are remained understudied. Herein we have highlighted leaves methanolic extract phytochemicals identification, antimicrobial, and antioxidant activity assessment. Cowpea leaves methanolic extract Liquid Chromatography-Mass Spectrometry (LC-MS) analysis first time revealed the presence of α-hederin, which is a putative novel SARS-COV-2 inhibitor and Zearlenone mycotoxin. Leaves methanolic extract exhibited strong activity against Streptococcus pyogens and Candida albicans. The Cowpea leaves extract is a potent DPPH inhibitor with an IC50 of 62.04 ± 0.08 µg/mL. The bioactive compounds identification in this work supports the plant's nutritional and medicinal uses.

Late recognition of Gore Helex atrial septal occluder fracture and migration.

Patent foramen ovale (PFO) closure is indicated in cryptogenic stroke. Percutaneous PFO closure is both feasible and highly efficacious with low incidence of device-related complications. When complications occur, they are usually discovered within 6 weeks of device deployment. We describe the case of a partially embolised and fractured Gore Helex Septal Occluder device recognised nearly 9 years after placement requiring surgical explant. .

Comparative Evaluation of Soft Tissue Chin Thickness in Adult Patients with Skeletal Class II Malocclusion with Various Vertical Growth Patterns: a Cephalometric Study.

INTRODUCTION: Facial soft tissue thickness is important not only for plastic surgeons but also for orthodontists to plan the treatment procedure. Genioplasty, an orthognathic surgery in combination with orthodontic treatment is indicated to restore adequate shape and projection of the chin in the face. It has been performed to enhance soft tissue contours related to disproportion between soft and hard tissue. These treatments require the critical information regarding the relation between soft and hard tissues for proper treatment plan-ning. However, there is very minimal documentation on comparison of soft tissue characteristics particularly in Class II malocclusion. AIM: To evaluate and compare soft tissue chin thickness in class II subjects with various growth patterns. To evaluate soft tissue chin thickness difference in males and females and compare the results with previous studies. MATERIALS AND METHODS: The study comprised 150 adults aged between 18 and 26 years (mean age 21 years). Based on FH/MP angle the study sample was allocated into three groups: group I - low (hypodivergent), group II - average, and group III - high (hyper-divergent). Radiographs were traced manually. Angular measurements were computed to determine the vertical position of the maxilla and mandible in relation to anterior cranial base, to true horizontal and to each other. Soft tissue chin thickness was measured at three different levels. RESULTS: Hyperdivergent group showed greater soft tissue chin thickness at Pog-Pog' than the hypodivergent and average angle groups. Hypodivergent group showed greater soft tissue chin thickness at Me-Me' and Gn-Gn' as compared to average and hyperdivergent groups. Males showed greater soft tissue chin thickness at hypodivergent, average and hyperdivergent group than females. CONCLUSIONS: Soft tissue thickness measurements were smaller in adult patients of hyperdivergent group compared to adult patients in clinically average and hypodivergent groups. All STC measurements were greater in men than in women. The findings suggested that STC thickness in hyperdivergent pattern should be considered differently at its most anterior point (Pog) relative to its inferior landmarks (Gn and Me).

Nutritional and phytochemical profiling of nutracereal finger millet (Eleusine coracana L.) genotypes.

Finger millet (Eleusine coracana L.) is gaining popularity as healthy food due to its nutritional and phytochemical properties. This study reports nutritional and phytochemical profile of ten finger millet genotypes. Proximate analysis of finger millet genotypes revealed moisture, total carbohydrate, protein, fat, fiber and ash in the range of 7.50-11.75, 71.90-76.38, 6.7-8.0, 1.2-1.7, 3.1-3.8 and 3.1-3.8 per cent respectively. Micro-nutrient profiling showed Ca, Fe, Zn, P, K and Mn in the range of 2400.00-3400.00, 40.28-47.60, 12.40-17.45, 1600.00-2900.00, 3800.00-5200.00 and 51.33-61.28 mg kg-1 respectively. Phytochemical profiling was done for total phenol, phytic acid, tannins, flavonoids, HCN, oxalate and trypsin inhibitor which were observed in the range of 99.75-112.25, 210.75-302.75, 340.00-500.00, 62.23-74.05, 2.45-2.80, 19.80-26.23 mg 100 g-1 and 207.35-234.23 TIU g-1 respectively. Amino acid profiling showed good amount of essential amino acids. Nutritional and phytochemical profiling of finger millet genotypes showed its potentiality to become source of health promoting food.

Zygote morphogenesis but not the establishment of cell polarity in Plasmodium berghei is controlled by the small GTPase, RAB11A.

Plasmodium species are apicomplexan parasites whose zoites are polarized cells with a marked apical organisation where the organelles associated with host cell invasion and colonization reside. Plasmodium gametes mate in the mosquito midgut to form the spherical and presumed apolar zygote that morphs during the following 24 hours into a polarized, elongated and motile zoite form, the ookinete. Endocytosis-mediated protein transport is generally necessary for the establishment and maintenance of polarity in epithelial cells and neurons, and the small GTPase RAB11A is an important regulator of protein transport via recycling endosomes. PbRAB11A is essential in blood stage asexual of Plasmodium. Therefore, a promoter swap strategy was employed to down-regulate PbRAB11A expression in gametocytes and zygotes of the rodent malaria parasite, Plasmodium berghei which demonstrated the essential role of RAB11A in ookinete development. The approach revealed that lack of PbRAB11A had no effect on gamete production and fertility rates however, the zygote to ookinete transition was almost totally inhibited and transmission through the mosquito was prevented. Lack of PbRAB11A did not prevent meiosis and mitosis, nor the establishment of polarity as indicated by the correct formation and positioning of the Inner Membrane Complex (IMC) and apical complex. However, morphological maturation was prevented and parasites remained spherical and immotile and furthermore, they were impaired in the secretion and distribution of microneme cargo. The data are consistent with the previously proposed model of RAB11A endosome mediated delivery of plasma membrane in Toxoplasma gondii if not its role in IMC formation and implicate it in microneme function.

Rural Set Up Experience of Viper Bite Treatment with Special Reference to FFP in Venom Induced Consumption Coagulopathy.

Snake bite is one of the major public health problems in India. Venom induced consumption coagulopathy(VICC) is the commonest coagulopathy resulting from viper bites. Anti-snake venom(ASV) is the only mainstay therapy in the management of snake bite. Despite anti-venom being efficacious and bonding to multiple toxins in the venom, there are number of reasons it may not be effective. The most important being irreversible toxic effects cannot be reversed by antivenom to toxin after damage has occurred, such as clotting factor deficiencies resulting from VICC. This study was done to evaluate the efficacy of use of anti-snake venom and ASV with fresh frozen plasma (FFP) in haemotoxic snake bites in a tertiary care hospital. Total 500 patients admitted during period from January 2010- April 2017 with history of snake bite. vasculotoxic[278], neurotoxic[126], localtoxic[64] and nontoxic[32]. Overall outcome in term of time recovery, renal complications, and death better in ASV plus FFP group. The complications due to snake bite were minimum, if anti snake venom was administered within first 4 hours.

Lipase catalyzed synthesis of antimicrobial andrographolide derivatives.

In this data article we describe screening of various lipases for the regioselective acylation of Andrographolide. Each lipase was screened with seven acyl donors. Amano lipase AK from Pseudomonas fluorescens was used for the synthesis of two new acylated andrographolide derivatives. Two new compounds, andrographolide-14-propionate and andrographolide-14-caproate were characterized by various spectral studies. These two derivatives showed more antimicrobial activity than andrographolide.

Regioselective and efficient enzymatic synthesis of antimicrobial andrographolide derivatives.

Labdane diterpene andrographolide (1) is a major constituent of Andrographis paniculata and known to exhibit wide spectrum of biological activities. In this study, regioselective monoesters of (1) have been synthesized by using Amano lipase AK (Pseudomonas fluorescens) as a biocatalyst. Amano lipase AK was able to execute highly efficient esterification of hydroxyl group attached to C-14 carbon of (1) in presence of acyl donors. Among the various synthesized derivatives including two novel compounds such as andrographolide-14-propionate (3) and andrographolide-14-caproate (5) displayed antimicrobial activity against Staphylococcus aureus with low minimal inhibitory concentration (MIC) 4 µg/mL and 16 µg/mL respectively. Furthermore, they have shown low hemolysis activity at their respective MIC and increase in the permeability of the bacterial cell membrane as delineated by FITC uptake and SEM imaging studies.

Severe skeletal Class II Division 1 malocclusion in postpubertal girl treated using Forsus with miniplate anchorage.

This case report outlines the treatment of a 17-year-old female with Class II Division 1 malocclusion with mandibular retrusion, deep bite, and convex facial profile. The Forsus fatigue resistance device with miniplate was used for this patient and it was very affecting tool in correcting both skeletal and dental parameters. The total active treatment time was 19 months. This method can serve as an alternate choice of treatment, especially those who refuse orthognathic surgery. Hence, Forsus with miniplate might be useful in both growing and postpubertal patients.

Clinician Corner- A Simple and Efficient Modified Goshgarian Arch ("T" Arch) Appliance for Correcting Single Tooth Scissor Bite.

Scissor bite is a rare form of malocclusion that is often accompanied by varying degrees of facial asymmetry. Correction and treatment of scissor bite in posterior teeth is a challenging job for an orthodontist. There are several treatment options for correction of scissor bite in posterior teeth but some have their own ill-effects on the dentition thus contributing further to the problem and some require patient compliance which compromises the outcome of the treatment. Therefore to overcome these drawbacks we have designed a new appliance to correct the molar scissor bite.

A Speedy Yet Simple Tip to Align Imbricated Anterior Teeth.

Correction of dental crowding can be achieved with several orthodontic procedures like extraction of teeth, proximal stripping etc. In contemporary orthodontic practice, resolution of incisor crowding is most commonly achieved by the use of canine lacebacks. This method takes time, requires frequent activations and taxes anchorage since it involves the "PULL" mechanics. In order to overcome these disadvantages, mostly the time factor, we devised a method of using open coil NiTi spring on 0.016" SS wire piggy backed on the 0.014" heat activated NiTi base arch wire ligated only at the canines. This method, resolved the incisor crowding in almost one third of the time taken with the use of canine lacebacks. Moreover, the result was more physiologic since light continuous force was applied with the spring and it essentially eliminated the anchorage loss since it involved "PUSH" mechanics.

Effect of Various Finishing Procedures on the Reflectivity (Shine) of Tooth Enamel - An In-vitro Study.

INTRODUCTION: Reflectivity of an object is a good parameter for surface finish. As the patient evaluates finishing as a function of gloss/reflectivity/shine an attempt is made here to evaluate changes in surface finish with custom made reflectometer. AIM: The aim of the present study was to study the effect of various procedures during orthodontic treatment on the shine of enamel, using a custom made reflectometer. MATERIALS AND METHODS: Sixty one extracted premolars were collected and each tooth was mounted on acrylic block. Reflectivity of the teeth was measured as compared to standard before any procedure. One tooth was kept as standard throughout the study. Sixty teeth were acid etched. Reflectivity was measured on custom made reflectometer and readings recorded. Same procedure was repeated after debonding. Then 60 samples were divided into three groups: Group 1 - Tungsten Carbide, Group 2 - Astropol, Group 3- Sof-Lex disc depending upon the finishing method after debonding and reflectivity was measured. RESULTS: The mean percentage of reflectivity after acid etching was 31.4%, debonding 45.5%, Tungsten carbide bur finishing (Group 1) was 58.3%, Astropol (Group 2) 72.8%, and Sof-Lex disc (Group 3) 84.4% as that to the standard. There was statistically very highly significant (p<0.001) difference in reflectivity restored by the three finishing materials in the study. Thus, the light reflection was better in Group 3> Group 2> Group 1. CONCLUSION: The primary goal was to restore the enamel to its original state after orthodontic treatment. The methods tested in this study could not restore the original enamel reflectivity.