Lactation performance and digestibility of forages and diets in dairy cows fed a hemicellulose extract.

Inclusion of hemicellulose extract (HE) in cattle diets have shown potential for improving fiber digestibility and production efficiency. The objective of this research was to evaluate production and digestibility effects of a HE on midlactation cows. Twelve multiparous Holstein cows (142 ± 44 d in milk, 685 ± 19 kg of body weight) including 4 with ruminal fistula were used in a 2 × 2 Latin square design with 21-d periods. Cows were fed a control (CON) diet containing 55% forage [dry matter (DM) basis, 2/3 corn silage and 1/3 alfalfa hay] or a similar diet where 1.0% of the diet DM was replaced with HE (TRT). Dry matter intake averaged 27.1 and 26.9 kg/d, for CON and TRT respectively, and was not affected by addition of extract. The percentage of milk protein (3.40 vs. 3.29%) was greater, whereas the percentage of milk fat (3.91 vs. 3.80%) tended to be greater, for cows fed the CON compared with the TRT diet. Because of numerically greater milk production (38.8 vs. 39.2 kg/d) for cows fed the TRT diet, no differences were observed in component yields other than lactose (1.86 vs. 1.94 kg/d), which tended to be greater for cows fed the TRT ration. Treatment improved neutral detergent fiber (NDF) digestibility (38.6 vs. 48.1%) for the TRT diet compared with the CON diet but did not affect apparent total-tract DM (67.8 vs. 68.5%), crude protein (67.2 vs. 67.9%), acid detergent fiber (ADF; 37.1 vs. 43.3%), or starch (92.8 vs. 92.2%) digestibility. For in situ determinations, Dacron bags containing corn silage, alfalfa hay, and either the CON or TRT ration were incubated in triplicate in the rumens of the cannulated cows at 0, 3, 6, 9, 12, 24, and 48 h on d 18 of each period. Each total mixed ration was incubated only in cows assigned to the corresponding diet. For corn silage, the rate of disappearance of NDF (1.70 vs. 4.27%) and ADF (1.79 vs. 4.66%) increased for cows fed the TRT diet. For alfalfa hay, the disappearance of fraction A of DM, NDF, and ADF decreased and fraction B of DM and NDF increased with treatment. The rate of disappearance for DM (8.03 vs. 11.04%), NDF (6.30 vs. 10.28%), and ADF (5.52 vs. 9.19%) increased for the alfalfa hay in rumens of treated cows. For the total mixed ration, the disappearance of the A fraction of NDF and ADF increased for cows fed the TRT diet. Supplementing diets of lactating dairy cows with an HE has beneficial effects on fiber degradation characteristics and provides opportunities for improving animal performance.

Effects of feeding propionate and calcium salts of long-chain fatty acids on transition dairy cow performance.

Multiparous Holstein cows (n = 40) were used in a randomized complete block design to determine the effects of feeding Ca and Na salts (1:1, wt/wt) of propionate and Ca salts of long-chain fatty acids (LCFA) on transition cow performance. All cows were fed the same basal diet once daily for ad libitum intake. Treatments (g/d) were 320 cornstarch (CS) as a control, 120 propionate (PRO), 120 propionate and 93 LCFA (PF1), and 178 propionate and 154 LCFA (PF2). Treatments were hand-mixed into the upper one-third of the TMR from 2 wk pre- through 3 wk postpartum. Intakes were recorded from 21 d pre- through 21 d postpartum. Energy density and crude protein were 1.54 and 1.65 Mcal/kg and 14.4 and 18.8% for pre- and postpartum diets, respectively. All cows received a common diet from 22 to 70 days in milk (DIM). Milk composition was analyzed on d 7, 14, and 21. Blood was sampled at 14, 7, and 2 d prepartum and 2, 7, 14, and 21 DIM. Pre- and postpartal dry matter intake (DMI) averaged 11.9 and 16.4 kg/d, respectively, and did not differ among treatments. A diet x week interaction for postpartal DMI was observed as cows fed PF2 consumed 2 kg/d less DM during wk 2 relative to other treatments. Milk yields from 22 to 70 DIM were 48.8, 48.5, 47.8, and 51.3 kg/d for CS, PRO, PF1, and PF2, respectively, and were not significantly affected by treatments. Milk true protein (3.32 vs. 3.16%) was increased and MUN (12.5 vs. 14.4 mg/dL) was decreased for CS relative to other treatments. Milk fat yield from cows fed PRO tended to be greater than those fed PF1 (1.58 vs. 1.29 kg/d). Plasma glucose, insulin, and beta-hydroxybutyrate were not affected by treatments. The PF2 treatment tended to decrease NEFA in plasma relative to PF1 over all times measured (492 and 670 muEq/L) and significantly decreased plasma NEFA relative to those fed PF1 postpartum (623 and 875 muEq/L). Relative to PF1, feeding propionate and LCFA at the higher level in this experiment improved energy balance postpartum as evidenced by decreased concentrations of plasma NEFA.

Effects of dietary glucogenic precursors and fat on feed intake and carbohydrate status of transition dairy cows.

Twenty-four multiparous Holstein cows were used to determine the effects of dietary fat and glucose precursors on energy status and lactation. The treatment group (T) received 409 g/d (DM basis) of a combination of calcium salts of fatty acids, calcium propionate, and propylene glycol. The control group (C) received 409 g/d of a mixture of calcium salts of fatty acids and ground barley from 14 +/- 0.9 g/d before until 21 d after calving. Dry matter intake was greater (16.1 vs. 13.6 +/- 1.3 kg/d) for T than C during the last week prepartum and did not decrease for T from the previous week, whereas, in C, DM intakes decreased by 3.2 kg/d. Production of milk and milk fat did not differ. There was a tendency for lower protein and increased lactose concentrations in milk from T cows. Milk fat percentage was lower in T at d 7 (5.5 vs. 6.4 +/- 0.5%) and 28 (4.4 vs. 5.5 +/- 0.5%) of lactation. Liver lipid content was numerically lower (7.9 vs. 9.2 +/- 0.9%) and glycogen content was significantly higher (2.4 vs. 2.0 +/- 0.1%) in T vs. C cows on d 7 of lactation. Concentrations of nonesterified fatty acids were lower in blood of T cows on d 2 and 7 of lactation. Over all time points, blood glucose concentrations were higher in T cows pre- (70.75 vs. 62.1 +/- 1.3 mg/dL) and postpartum (60.1 vs. 56.2 +/- 1.1 mg/dL). Insulin concentrations in blood were greater for T (397 vs. 314 +/- 48 pg/mL) both pre- and postpartum. Feeding glucose precursors in combination with rumen inert lipids, compared with feeding barley in combination with the lipids for 2 wk before parturition and 3 wk postpartum helped avoid prepartum feed intake depression and increased blood glucose and insulin and decreased blood NEFA.

Genetic polymorphism of PAS-I, the mucin-like glycoprotein of bovine milk fat globule membrane.

A sensitive analytical method based on gel electrophoresis and silver staining was developed to detect PAS-I, a glycoprotein of bovine milk fat globule membrane. Application of the method to samples of milk from individual animals demonstrated that PAS-I is polymorphic and established that it also occurs in the skim milk phase. This polymorphism consisted of two bands showing variable mobility among samples of individual animals. Band patterns for an individual persisted from one lactation to the next. Comparison of 12 dam-daughter pairs for PAS-I patterns indicated that each pair had at least one band matching in mobility. Milk from identical twins had identical PAS-I patterns. Two out of three sets of fraternal twins had one band that did not match. Based on these data and genetics established for a similar protein of human milk, two codominant alleles, one from the sire and the other from the dam, account for the two bands of PAS-I. The PAS-I band patterns may be related to inheritance of milk production and composition factors.