Impact of Camellia sinensis Iron Oxide Nanoparticle on Growth, Hemato-biochemical and Antioxidant Capacity of Blue Gourami (Trichogaster trichopterus) Fingerlings.

The effect of green tea (Camellia sinensis) iron oxide nanoparticles (nano-Fe) on the effectiveness, growth, antioxidant capacity, and immunological response of Trichogaster trichopterus (Blue gourami) fingerlings was investigated. UV-Visible, Fourier Transform Infrared, Scanning Electron Microscopy, Energy Dispersive X-ray, X-ray diffraction, Dynamic Light Scattering, and Zeta Potential spectroscopy were used to evaluate the biologically synthesized nano-Fe. Characterization revealed the hexagonal and spherical morphology with an average diameter of 114 nm. Six different experimental diets were supplied to the fish in duplicate for 60 days. The first diet served as a control (no nano-Fe supplementation), whereas the remaining five diets contained nano-Fe at concentrations of 10, 20, 30, 40, and 50 mg/kg (D1 to D5). The results indicated that fish fed a nano-Fe diet at a concentration of 40 mg/kg had improved growth performance, biochemical constituents, hematological parameters, and antioxidant activity in T. trichopterus, implying that it might be used as a vital feed supplement in ornamental fish culture.

Isolation, Expansion, and Characterization of Placenta Originated Decidua Basalis-Derived Mesenchymal Stromal Cells.

Mesenchymal stromal cells (MSCs) were isolated from Decidua Basalis (DB) and studied for their final cellular product measures, such as safety, purity, quality, quantity, and integrity that are ascribed as cellular products. This research aimed to isolate MSCs for expansion under the clinical scale level with potency, secretion of cytokines, growth factors secreted by DB-MSCs, and their role in wound healing. Placentas isolated from DB were expanded up to the 10th passage, and their characteristics were assessed by phenotypic characterization using a flow cytometer and analyzed for trilineage differentiation by cytochemical staining. Growth factors (GF), interleukins (IL), chemokines, and tissue inhibitors of metalloproteinases (TIMP) were measured with enzyme-linked immunosorbent assays. The harvested cells from the placenta yield 1.63-2.45 × 104cells/cm2 at P(0), 3.66-5.31 × 104cells/cm2 at P(1), 4.01-5.47 × 104cells/cm2 at P(2), and 3.94-5.60 × 104cells/cm2 at P(10) accordingly; up to 4.74 × 109 P(2) DB-MSCs were harvested within 9-11 days. The viability of the freshly harvested cells was greater than 90% in all cases. It is able to differentiate into chondrocytes, adipocytes, and osteogenic cells, proving their ability to differentiate into a trilineage. Thus, this study put an insight into a secure and conventional approach toward their ability to differentiate into multiple lineages and secrete factors related to immune regulation, making DB-MSCs a potential source in various therapeutic applications.