Fish-borne nematodiases in South America: neglected emerging diseases.

Fish-borne zoonotic nematodes may infect humans when fish or squid are ingested raw or inadequately cooked. Human infections may have serious consequences, including the unexpected deaths of infected people. This kind of disease is poorly known in general, and the characteristics of such infections in South American countries as a whole have never been assessed. In this paper the present status of fish-borne nematodiases in humans in South American countries is characterized. Potentially zoonotic nematode species are very common in both freshwater and marine fish in South America. Reports of human infections have only been found in some countries, and their incidence (especially with anisakids and Gnathostoma spp.) varies from country to country. Apparently they are more abundant in countries with strong traditions of eating raw fish, and are more frequent on the western coast of South America. So far fish-borne nematodes have been reported in Argentina, Brazil, Chile, Colombia, Ecuador and Peru. In recent years, cases of human infection have appeared in probably underestimated numbers. People need to be clearly informed about risky feeding habits, and physicians need to learn more about zoonotic diseases.

New species of Cucullanus (Nematoda: Cucullanidae), an intestinal parasite of the peacock bass Cichla piquiti (Perciformes: Cichlidae) from the Tocantins River, Brazil.

Cucullanus tucunarensis n. sp. (Cucullanidae) is described parasitizing the peacock bass Cichla piquiti (Cichlidae) from the Tocantins River, Tocantins State, Brazil. The new species is unique and differs from all its congeners by having a tail tip provided with several sclerotized spine-like processes on its ventral side, present in both the male and female. Furthermore, C. tucunarensis n. sp. is compared with other species of the genus recorded in the same zoogeographical region and in the same group of hosts (Perciformes).

The impact of a dam on the helminth fauna and health of a neotropical fish species Salminus brasiliensis (Cuvier 1816) from the upper Paraná River, Brazil.

The aim of the present study was to detect changes in the structure of the helminth parasite infracommunities in Salminus brasiliensis (Cuvier 1816) from the floodplain of the upper Paraná River after construction of the Porto Primavera Hydroelectric Plant. A total of 126 fish in the period before the dam's construction and 56 specimens 10 years after this event were analysed. Three species of parasites were collected before the construction of the dam: Prosthenhystera obesa Diesing, 1850 (Digenea), Cladocystis intestinalis Vaz, 1932 (Digenea) and Monticellia coryphicephala Monticelli, 1892 (Cestoda), and one nematode species in the larval stage, whose identification was not possible. After dam construction, the following helminth parasites were found: C. intestinalis, M. coryphicephala, Octospiniferoides incognita, Contracaecum spp. larvae and Contracaecum sp. type 2 larvae Moravec, Kohn & Fernandes 1993. The diversity of helminth parasites measured by the Brillouin diversity index (HB) differed significantly between the pre- and post-dam periods (mean HB = 0.069 and HB = 0.2, respectively; P= 0.0479; Mann-Whitney U test). The parasite community of S. brasiliensis before the construction of the dam showed concentration of dominance (C) of P. obesa (C = 0.38), while there was no concentration of dominance of any species of parasite (C = 0.22) after the dam's construction. Before the Porto Primavera dam the relative condition factor of fish was 1.0; after the dam's construction it was 0.93 (P < 0.0001; Mann-Whitney U test). This study records the disappearance of the species P. obesa and suggests that there has been local extinction of this parasite. The results show that the anthropic influence on natural systems is interfering with the welfare and health of S. brasiliensis, reflected by its fauna of helminth parasites.

Comparative parasitism of the fish Plagioscion squamosissimus in native and invaded river basins.

Biological invasions are considered a major threat to biodiversity around the world, but the role of parasites in this process is still little investigated. Here, we compared parasite infections of a host species in the areas where it originated and where it was introduced, and in native and introduced species in the same environment, using the endoparasites of the fish Plagioscion squamosissimus (Sciaenidae) in 3 Brazilian basins. Samples were taken in 2 rivers where the species is native, i.e., Solimões River (SO) and Tocantins River (TO), and where the species was introduced, the upper Paraná River (PR). In addition, abundances of diplostomids and larval nematodes were compared between P. squamosissimus and 2 native competitors in the PR, Hoplias malabaricus and Raphiodon vulpinus. In total, 13 species of endoparasites were recorded, but only Austrodiplostomum sp. and cestode cysts were present in all localities. Although infracommunity richness was similar, their species composition was slightly different among localities. General linear models using the relative condition factor of fish as response variables, and abundance of the most prevalent parasites as possible predictors showed that the condition of fish is negatively correlated with parasite abundance only in the native range (TO). Abundance of diplostomid eye flukes was higher in the PR, and in the native species H. malabaricus when compared to the invader, which might present an advantage for P. squamosissimus if they compete for prey. However, although P. squamosissimus may have lost some of its native parasites during its introduction to the PR, it is now possibly acting as a host for native generalist parasites.

Diversity of parasites of fish from the Upper Paraná River floodplain, Brazil.

The aim of the present study was to provide a current survey of the species of parasites found in the Upper Paraná River floodplain, as well as to investigate which strategies and mechanisms used by parasites, are favored and which environmental factors influence the parasite community in the studied environments. During a seven-year period from February 2000 to September 2007, 3,768 fish belonging to 72 species were collected and analyzed for the purpose of studying the parasite fauna. A total of 337 species of parasites were reported, including 12 new descriptions: one myxosporid, Henneguya paranaensis Eiras, Pavanelli and Takemoto, 2004; eight monogeneans, Kritskyia annakohnae Boeger, Tanaka and Pavanelli, 2001; Kritskyia boegeri Takemoto, Lizama and Pavanelli, 2002; Kritskyia eirasi Guidelli, Takemoto and Pavanelli, 2003; Demidospermus labrosi França, Isaac, Pavanelli and Takemoto, 2003; Demidospermus mandi França, Isaac, Pavanelli and Takemoto, 2003; Pseudovancleaveus paranaensis França, Isaac, Pavanelli and Takemoto, 2003; Tereancistrum curimba Lizama, Takemoto and Pavanelli, 2004 and Tereancistrum toksonum Lizama, Takemoto and Pavanelli, 2004; two digeneans, Sanguinicola platyrhynchi Guidelli, Isaac and Pavanelli, 2002 and Dadayius pacupeva Lacerda, Takemoto and Pavanelli, 2003 and one cestode, Nomimoscolex pertierrae Chambrier, Takemoto and Pavanelli, 2005. In addition, several other species were reported for the first time in new hosts or in the floodplain. Monogeneans presented the highest number of species, followed by digeneans. The infection site with the highest species richness was the intestine, with 127 species.

Ecology of endoparasites of the fluvial stingray Potamotrygon falkneri (Chondrichthyes: Potamotrygonidae) from the upper Paraná River floodplain, Brazil.

The present study investigated the ecological relationships between endoparasites and the host Potamotrygon falkneri from the upper Paraná River by using as indicators the host's relative condition factor (Kn), sex and hepatosomatic relation (HSR). Forty-seven specimens of P. falkneri were analyzed between March 2005 and September 2006. Statistical analysis showed that the Kn was positively correlated with the abundance of Acanthobothrium regoi and Rhinebothrium paratrygoni; only the abundance of A. regoi was positively correlated to the HSR, whereas R. paratrygoni did not present correlation and there was no influence of the host's sex on the abundance and prevalence of parasites. This is the first study concerning the ecology of parasites of potamotrygonids.

Diversity of parasites of fish from the Upper Paraná River floodplain, Brazil / Diversidade dos parasitos de peixes da planície de inundação do Alto Rio Paraná, Brasil

The aim of the present study was to provide a current survey of the species of parasites found in the Upper Paraná River floodplain, as well as to investigate which strategies and mechanisms used by parasites, are favored and which environmental factors influence the parasite community in the studied environments. During a seven-year period from February 2000 to September 2007, 3,768 fish belonging to 72 species were collected and analyzed for the purpose of studying the parasite fauna. A total of 337 species of parasites were reported, including 12 new descriptions: one myxosporid, Henneguya paranaensis Eiras, Pavanelli and Takemoto, 2004; eight monogeneans, Kritskyia annakohnae Boeger, Tanaka and Pavanelli, 2001; Kritskyia boegeri Takemoto, Lizama and Pavanelli, 2002; Kritskyia eirasi Guidelli, Takemoto and Pavanelli, 2003; Demidospermus labrosi França, Isaac, Pavanelli and Takemoto, 2003; Demidospermus mandi França, Isaac, Pavanelli and Takemoto, 2003; Pseudovancleaveus paranaensis França, Isaac, Pavanelli and Takemoto, 2003; Tereancistrum curimba Lizama, Takemoto and Pavanelli, 2004 and Tereancistrum toksonum Lizama, Takemoto and Pavanelli, 2004; two digeneans, Sanguinicola platyrhynchi Guidelli, Isaac and Pavanelli, 2002 and Dadayius pacupeva Lacerda, Takemoto and Pavanelli, 2003 and one cestode, Nomimoscolex pertierrae Chambrier, Takemoto and Pavanelli, 2005. In addition, several other species were reported for the first time in new hosts or in the floodplain. Monogeneans presented the highest number of species, followed by digeneans. The infection site with the highest species richness was the intestine, with 127 species.

O objetivo do presente trabalho foi realizar um novo levantamento das espécies de parasitos encontradas na planície de inundação do Alto Rio Paraná, bem como investigar quais estratégias e mecanismos utilizados pelos parasitos são mais favorecidos e quais fatores ambientais estão influenciando a comunidade parasitária nos ambientes estudados. Durante o período de fevereiro de 2000 a setembro de 2007, 3.768 espécimes de peixes pertencentes a 72 espécies foram coletados e analisados, visando o estudo da fauna parasitária. Até o momento foram registradas, 337 espécies de parasitos, sendo 12 novas espécies: uma de mixosporídeo, Henneguya paranaensis Eiras, Pavanelli e Takemoto, 2004; oito de monogenéticos, Kritskyia annakohnae Boeger, Tanaka e Pavanelli, 2001; Kritskyia boegeri Takemoto, Lizama e Pavanelli, 2002; Kritskyia eirasi Guidelli, Takemoto e Pavanelli, 2003; Demidospermus labrosi França, Isaac, Pavanelli e Takemoto, 2003; Demidospermus mandi França, Isaac, Pavanelli e Takemoto, 2003; Pseudovancleaveus paranaensis França, Isaac, Pavanelli e Takemoto, 2003; Tereancistrum curimba Lizama, Takemoto e Pavanelli, 2004 e Tereancistrum toksonum Lizama, Takemoto e Pavanelli, 2004; duas de digenéticos, Sanguinicola platyrhynchi Guidelli, Isaac e Pavanelli, 2002 e Dadayius pacupeva Lacerda, Takemoto e Pavanelli, 2003 e uma de cestóide, Nomimoscolex pertierrae Chambrier, Takemoto e Pavanelli, 2005. Além dessas novas espécies, várias outras foram registradas pela primeira vez em novos hospedeiros ou na planície. O grupo dos monogenéticos foi o que apresentou maior número de espécies encontradas, seguido pelos digenéticos. O intestino foi o sítio de infecção que apresentou a maior riqueza, com 127 espécies.

Ecology of endoparasites of the fluvial stingray Potamotrygon falkneri (Chondrichthyes: Potamotrygonidae) from the upper Paraná River floodplain, Brazil / Ecologia de endoparasitos da raia fluvial Potamotrygon falkneri (Chondrichthyes: Potamotrygonidae) da planície de inundação do alto rio Paraná, Brasil

The present study investigated the ecological relationships between endoparasites and the host Potamotrygon falkneri from the upper Paraná River by using as indicators the host's relative condition factor (Kn), sex and hepatosomatic relation (HSR). Forty-seven specimens of P. falkneri were analyzed between March 2005 and September 2006. Statistical analysis showed that the Kn was positively correlated with the abundance of Acanthobothrium regoi and Rhinebothrium paratrygoni; only the abundance of A. regoi was positively correlated to the HSR, whereas R. paratrygoni did not present correlation and there was no influence of the host's sex on the abundance and prevalence of parasites. This is the first study concerning the ecology of parasites of potamotrygonids.

O presente estudo investigou as relações ecológicas entre os endoparasitos e os hospedeiros Potamotrygon falkneri do alto rio Paraná, utilizando como indicadores o fator de condição relativo (Kn), sexo e relação hepatossomática (HSR) dos hospedeiros. Entre março de 2005 e setembro de 2006, 47 espécimes de P. falkneri foram analisados. As análises mostraram que o Kn estava positivamente correlacionado com a abundância de Acanthobothrium regoi e Rhinebothrium paratrygoni; apenas a abundância de A. regoi estava correlacionada positivamente com a HSR, enquanto R. paratrygoni não apresentou correlação e não houve influência do sexo do hospedeiro na abundância e prevalência dos parasitos. Este é o primeiro estudo sobre ecologia de parasitos de potamotrigonídeos.

Henneguya corruscans n. sp. (Myxozoa, Myxosporea, Myxobolidae), a parasite of Pseudoplatystoma corruscans (Osteichthyes, Pimelodidae) from the Paraná River, Brazil: a morphological and morphometric study.

This paper describes the parasite Henneguya corruscans n. sp. which infects the gills of Pseudoplatystoma corruscans Spix and Agassiz, 1829 found in the Paraná River, Brazil. The parasites belong to the interlamellar-epithelial type as defined by Molnár (2002) [Molnár, K., 2002. Site preference of fish myxosporeans in the gills. Dis. Aquat. Org. 48, 197-207]. The spores examined had thin, smooth walls with symmetric valves; the total length of the spores was 27.6 (25-29)mum. The spore body was ellipsoidal in frontal view and biconvex in lateral view and they measured 14.3 (13-15)mum long by 5mum wide and 4mum in thickness. The polar capsules were small and elongated, equally sized, with a rounded posterior extremity and tapering anteriorly, and they corresponded more or less the half the length of the spore body; they were 6.8 (6-7)mum long by 2mum wide, and the polar filament formed 5-6 coils obliquely to the axis of the polar capsule. The tail was 13.7 (12-15)mum long and bifurcated shortly after the end of the spore body. The importance of the infection for the farming of P. corruscans is discussed.

Helminths parasitizing larval fish from Pantanal, Brazil.

Fish larvae of 'corvinas' (Pachyurus bonariensis and Plagioscion ternetzi) from Sinhá Mariana Lagoon, Mato Grosso State, were collected from March 2000 to March 2004, in order to determine the parasitic fauna of fishes. Larvae from the two species were parasitized by the same endoparasites: Contracaecum sp. Type 2 (larvae) (Nematoda: Anisakidae) in the mesentery and Neoechinorhynchus (Neoechinorhynchus) paraguayensis (Acanthocephala: Neoechinorhynchidae) in the stomach and the terminal portion of the intestine. Statistical analysis showed that there was a significant positive correlation between the standard length of hosts and the abundance of acanthocephalans and nematodes, and that the prevalence of nematodes presented a significant positive correlation with the standard length of the two species of hosts, indicating the presence of a cumulative process of infection. The present study constitutes the first record of nematodes and acanthocephalans parasitizing larval fish, as well as the first record of endoparasites in fish larvae in Brazil. In addition, it lists a new locality and two species of hosts for Contracaecum sp. Type 2 (larva) and N. (N.) paraguayensis.

Ecology of Clinostomum complanatum Rudolphi, 1814 (Trematoda: Clinostomidae) infecting fish from the floodplain of the high Paraná River, Brazil.

The prevalence of Clinostomum complanatum Rudolphi, 1814 (Digenea, Clinostomidae) in fishes Loricariichthys platymetopon, Parauchenipterus galeatus and Hoplosternum littorale, which are second intermediate hosts, was studied at the floodplain of the high Paraná River, Brazil. Season (alternation flood-drought), habitat (lentic and semi-lotic), and sex were not related to its prevalence. For L. platymetopon, the immature and smaller fish had the lowest prevalence, whilst the opposite was observed for P. galeatus and H. littorale. This suggests that the probability of being predated is unchanged by parasitism for L. platymetopon; thus, a cumulative effect of repeated infections is observed; for the two other species, the highest parasitised fish may have higher predation mortality rates. While H. littorale is the preferred item in birds' diet, L. platymetopon is the most abundant fish species and has the highest C. complanatum prevalence, which makes it the most likely path of transmission to the bird, the definitive hosts of C. complanatum.

Diplostomum (Austrodiplostomum) compactum (Lutz, 1928) (Platyhelminthes, Digenea) metacercariae in fish from the floodplain of the Upper Paraná River, Brazil.

Diplostomum (Austrodiplostomum) compactum (Lutz, 1928) metacercariae (Platyhelminthes, Digenea) were found in six fish species, belonging to two orders (Characiformes and Perciformes) and three families (Erythrinidae, Sciaenidae and Cichlidae). A total of 477 individuals were collected, from August 1999 to May 2001, in the upper Paraná River floodplain, Brazil. The metacercariae were infecting the eyes of six host species and the brain of five of them. Plagioscion squamosissimus, Satanoperca pappaterra and Cichla monoculus presented the highest values of prevalence, mean intensity and mean abundance. Diplostomum (A.) compactum was over-dispersed in the population of Hoplias aff. malabaricus, S. pappaterra, P. squamosissimus, Crenicichla britskii and C. monoculus. No significant difference was observed in the metacercariae distribution in the right and left eyes. The abundance of D. (A.) compactum metacercariae was positively correlated with the host's relative condition factor in H. aff. malabaricus and S. pappaterra. No significant difference of the abundance of parasitism between the sex of the hosts was evidenced. In relation to the prevalence, significant difference was observed only in C. britskii. Positive correlations were verified between the prevalence of infection and the standard length of the specimens in C. britskii and the intensity of infection and the standard length of the hosts in P. squamosissimus and C. monoculus. In relation to parasite abundance and the standard length class of the host, significant differences were observed in P. squamosissimus, C. britskii and C. monoculus.

Myxobolus insignis sp. n. (Myxozoa, Myxosporea, Myxobolidae), a parasite of the Amazonian teleost fish Semaprochilodus insignis (Osteichthyes, Prochilodontidae).

A new myxosporean species is described from the fish Semaprochilodus insignis captured from the Amazon River, near Manaus. Myxobolus insignis sp. n. was located in the gills of the host forming plasmodia inside the secondary gill lamellae. The spores had a thick wall (1.5-2 microm) all around their body, and the valves were symmetrical and smooth. The spores were a little longer than wide, with rounded extremities, in frontal view, and oval in lateral view. They were 14.5 (14-15) microm long by 11.3 (11-12) microm wide and 7.8 (7-8) microm thick. Some spores showed the presence of a triangular thickening of the internal face of the wall near the posterior end of the polar capsules. This thickening could occur in one of the sides of the spore or in both sides. The polar capsules were large and equal in size surpassing the midlength of the spore. They were oval with the posterior extremity rounded, and converging anteriorly with tapered ends. They were 7.6 (7-8) microm long by 4.2 (3-5) microm wide, and the polar filament formed 6 coils slightly obliquely to the axis of the polar capsule. An intercapsular appendix was present. There was no mucous envelope or distinct iodinophilous vacuole.

Myxobolus insignis sp. n. (Myxozoa, Myxosporea, Myxobolidae), a parasite of the Amazonian teleost fish Semaprochilodus insignis (Osteichthyes, Prochilodontidae)

A new myxosporean species is described from the fish Semaprochilodus insignis captured from the Amazon River, near Manaus. Myxobolus insignis sp. n. was located in the gills of the host forming plasmodia inside the secondary gill lamellae. The spores had a thick wall (1.5-2 µm) all around their body, and the valves were symmetrical and smooth. The spores were a little longer than wide, with rounded extremities, in frontal view, and oval in lateral view. They were 14.5 (14-15) µm long by 11.3 (11-12) µm wide and 7.8 (7-8) µm thick. Some spores showed the presence of a triangular thickening of the internal face of the wall near the posterior end of the polar capsules. This thickening could occur in one of the sides of the spore or in both sides. The polar capsules were large and equal in size surpassing the midlength of the spore. They were oval with the posterior extremity rounded, and converging anteriorly with tapered ends. They were 7.6 (7-8) µm long by 4.2 (3-5) µm wide, and the polar filament formed 6 coils slightly obliquely to the axis of the polar capsule. An intercapsular appendix was present. There was no mucous envelope or distinct iodinophilous vacuole.

Host population density as the major determinant of endoparasite species richness in floodplain fishes of the upper Paraná River, Brazil.

A comparative analysis of parasite species richness was performed across 53 species of fish from the floodplain of the upper Paraná River, Brazil. Values of catch per unit effort, CPUE (number of individuals of a given fish species captured per 1000 m(2) of net during 24 h) were used as a rough measure of population density for each fish species in order to test its influence on endoparasite species richness. The effects of several other host traits (body size, social behaviour, reproductive behaviour, spawning type, trophic category, feeding habits, relative position in the food web, preference for certain habitats and whether the fish species are native or exotic) on metazoan endoparasite species richness were also evaluated. The CPUE was the sole significant predictor of parasite species richness, whether controlling for the confounding influences of host phylogeny and sampling effort or not. The results suggest that in the floodplain of the upper Paraná River (with homogeneous physical characteristics and occurrence of many flood pulses), population density of different host species might be the major determinant of their parasite species richness.

Influence of host sex and age on infracommunities of metazoan parasites of Prochilodus lineatus (Valenciennes, 1836) (Prochilodontidae) of the upper Paraná River floodplain, Brazil.

149 specimens of Prochilodus lineatus were collected on the upper Paran6 River floodplain. Of these, 121 (82.1%) were parasitized. 33 species of metazoan parasites were recorded. Rhinonastes pseudocapsaloideum was classified as secondary, while all other species were classified as satellites. Ergasilus sp. and Tereancistrum curimba were positively associated and their abundances were positively correlated. Saccocoelioides magnorchis and S. nanii were positively associated and their abundances were positively correlated. The mean diversity in the infracommunities of P. lineatus was H = 0.6875 +/- 0.4398. Host standard length was not correlated with parasite diversity (rs = 0.1726; p = 0.0533). The abundances of T. curimba and Kritskyia boegeri, and S. magnorchis and S. nanii were significantly correlated with host length. Correlation between fish age and parasite prevalence was not significant. The abundances of Amplexibranchius sp., K. boegeri and S. magnorchis were significantly different among host age classes. For Amplexibranchius sp., the three-year old age class had more parasites. For K. boegeri, the intermediate age classes had the most numerous parasites. Only Tereancistrum curimba showed significant difference in the prevalence between the sexes, with females being more parasitized.

Henneguya schizodon n. sp. (Myxozoa, Myxobolidae), a parasite of the Amazonian teleost fish Schizodon fasciatus (Characiformes, Anostomidae).

A new histozoic species of myxosporean (Henneguya schizodon n. sp.) is described from the Amazon River teleost fish Schizodon fasciatus Spix & Agassiz, 1892 (Characiformes, Anostomidae). The plasmodia, which showed asynchronous development, were located in the kidney of the host. The spore body was ellipsoidal and was 13.1 (12-14) micron long by 3.3 (3-4) micron wide. The total length of the spore was 28.9 (27-30) micron, and each value had a caudal process measuring 16.3 (15-17) micron. The polar capsules were 5.4 (5-6) micron long by 1.3 (1-1.5) micron wide, and each had a polar filament with 8-10 coils. The characteristics of the species were compared with nearly all the species described so far, including all the species reported from South American fishes. This comparison allows to consider the materials as a new species, and the name Henneguya schizodon n. sp. is proposed.

Endoparasite infracommunities of Hemisorubim platyrhynchos (Valenciennes, 1840) (Pisces: Pimelodidae) of the Baía River, Upper Parana River floodplain, Brazil: specific composition and ecological aspects.

Parasites of 136 specimens of Hemisorubim platyrhynchos, popularly called jurupoca, were analyzed. Fourteen parasite species were recorded: four digeneans (Crocodilicola pseudostoma, one Gorgoderidae species, Sanguinicola platyrhynchi, and Sphincterodiplostomum sp.); three cestodes (Goezeella paranaensis, Spatulifer maringaensis, and Mariauxiella piscatorum); five nematodes (Contracaecum Type 1 larvae of Moravec, Kohn, & Fernandes, 1993; Contracaecum Type 2 larvae of Moravec, Kohn, & Fernandes, 1993; Cucullanus (Cucullanus) zungaro; Eustrongylides ignotus; and Goezia sp.); one acanthocephalan (Quadrigyrus machadoi), and one pentastomid (Sebekia sp.). Most of the helminth specimens were found in larval stages, confirming that H. platyrhynchos is a significant source of their transmission. No dominance was reported. With the exception of Contracaecum Type 2 (featuring random dispersion), the species had a clumped pattern of dispersion in the host sample. Evidence of competition among parasite species was not reported. Several species of parasites were correlated with host size and sex. This fact may be explained by increase in food content and possible behavioral modifications of male and female hosts. Infracommunity diversity was not related to host size or sex.

The attachment of Clinostomum sp. (Digenea, Clinostomidae) to the oesophagus of the bird Ardea cocoi (Aves, Ardeidae).

The attachment of Clinostomum sp. to the oesophagus of the bird definitive host Ardea cocoi is reported. The parasite attaches to the mucous layer of the oesophagus by the acetabulum, oral sucker, and oral collar. The mucous layer of the oesophagus engulfed by the oral sucker presents an intense liquefactive necrosis, and the engulfment is not likely to contribute significantly to the attachment of the parasite. The oral collar of the parasite, surrounding the oral sucker, is juxtaposed to the oesophagus mucous layer, showing a perfect fitting of both surfaces. It is concluded that the main attachment mechanism of the parasites to the oesophagus of the host is provided by the acetabulum and by the juxtaposition of the oral collar of the parasites to the oesophagus host surface.

Endoparasite infracommunities of Hemisorubim platyrhynchos (Valenciennes, 1840) (Pisces: Pimelodidae) of the Baía River, upper Paraná River floodplain, Brazil: specific composition and ecological aspects

Parasites of 136 specimens of Hemisorubim platyrhynchos, popularly called jurupoca, were analyzed. Fourteen parasite species were recorded: four digeneans (Crocodilicola pseudostoma, one Gorgoderidae species, Sanguinicola platyrhynchi, and Sphincterodiplostomum sp.); three cestodes (Goezeella paranaensis, Spatulifer maringaensis, and Mariauxiella piscatorum); five nematodes (Contracaecum Type 1 larvae of Moravec, Kohn, & Fernandes, 1993; Contracaecum Type 2 larvae of Moravec, Kohn, & Fernandes, 1993; Cucullanus (Cucullanus) zungaro; Eustrongylides ignotus; and Goezia sp.); one acanthocephalan (Quadrigyrus machadoi), and one pentastomid (Sebekia sp.). Most of the helminth specimens were found in larval stages, confirming that H. platyrhynchos is a significant source of their transmission. No dominance was reported. With the exception of Contracaecum Type 2 (featuring random dispersion), the species had a clumped pattern of dispersion in the host sample. Evidence of competition among parasite species was not reported. Several species of parasites were correlated with host size and sex. This fact may be explained by increase in food content and possible behavioral modifications of male and female hosts. Infracommunity diversity was not related to host size or sex