RESUMEN
Results of treatment of were analysed 30 patients over a long nonhealing wounds and trophic ulcers, in which as a topical treatment, the method of vacuum therapy was applied. To study the activity of reparative regeneration in the wound using a dynamic method of determining the area of wounds using lqSquare computer program and immunohistochemical method for determining the Ki 67 positive cells in tissues of near wound area. It was found that lowdose exposure to vacuum at the wound contributed to increase the proliferative activity of tissues of near wound area and reduce the area of wound defect. Assessment of changes in the area of the wound surface is an important predictor of treatment efficacy, prevention of complications.
Asunto(s)
Infecciones por Bacterias Gramnegativas/terapia , Infecciones por Bacterias Grampositivas/terapia , Terapia de Presión Negativa para Heridas/métodos , Úlcera Cutánea/terapia , Traumatismos de los Tejidos Blandos/terapia , Infección de Heridas/terapia , Antibacterianos/uso terapéutico , Biomarcadores/metabolismo , Desbridamiento/métodos , Expresión Génica , Infecciones por Bacterias Gramnegativas/diagnóstico por imagen , Infecciones por Bacterias Gramnegativas/patología , Infecciones por Bacterias Gramnegativas/cirugía , Infecciones por Bacterias Grampositivas/diagnóstico por imagen , Infecciones por Bacterias Grampositivas/patología , Infecciones por Bacterias Grampositivas/cirugía , Humanos , Interpretación de Imagen Asistida por Computador , Inmunohistoquímica , Antígeno Ki-67/genética , Antígeno Ki-67/metabolismo , Úlcera Cutánea/diagnóstico por imagen , Úlcera Cutánea/patología , Úlcera Cutánea/cirugía , Traumatismos de los Tejidos Blandos/diagnóstico por imagen , Traumatismos de los Tejidos Blandos/patología , Traumatismos de los Tejidos Blandos/cirugía , Legrado por Aspiración/métodos , Infección de Heridas/diagnóstico por imagen , Infección de Heridas/patología , Infección de Heridas/cirugíaRESUMEN
The aim of our study was to investigate the role of intercellular mediators interleukin-1 receptor antagonist (IL-1 RA), interleukin-17 (IL-17), receptor activator of nuclear factor kB ligand (RANKL) and osteoprotegerin in the mechanisms of metabolic regulation of renal and bone tissue on model of violations of bone remodeling in chronic kidney disease (CKD). It was found a significant increase in the content of cytokines IL-1 RA (4,207 ± 0,546 pg/ml), IL-17 (33,944 ± 0,938 pg/ ml), osteoprotegerin (28,338 ± 1,223 pg/ml) and RANKL (0,184 ± 0,018 pmol/l) in the serum of animals in violation of bone remodeling in CKD compared with the contents of the studied cytokines in animals in the control group (2,529 ± 0,132 pg/ml, 28,166 ± 0,526 pg/ml, 21,588 ± 0,763 pg/ml and 0,131 ± 0,006 pmol/l, respectively) (P<0.05). The obtained correlations reflect the relationship between regulation of bone remodeling and the development of inflammation in CKD. The imbalance between pro- and anti-inflammatory cytokine plays an important role both in the development of CKD and in processes of bone remodeling.
Asunto(s)
Remodelación Ósea/inmunología , Citocinas/sangre , Riñón/patología , Osteoclastos/patología , Insuficiencia Renal Crónica/patología , Animales , Modelos Animales de Enfermedad , Femenino , Interleucina-17/sangre , Riñón/inmunología , Osteoclastos/inmunología , Osteoprotegerina/sangre , Ligando RANK/sangre , Ratas , Receptores de Interleucina-1/sangre , Insuficiencia Renal Crónica/inmunologíaRESUMEN
The pronounced dysbalance of the cytokines profiles in the blood of patients, suffering recurrent hydronephrosis, caused by pelvio-ureteric segment stenosis of various etiology and in different clinical course, in the inborn obstruction especially, was revealed on a 21th postoperative day, witnessing the existence of various ways of the stricture recurrence occurrence. As a prognostic criterion of risk of the recurrence occurrence there were proposed: a ratio of level of a tumor necrosis factor-alpha (TNF-alpha) to interleukin-10 (IL-10) level, and as an additional diagnostic criterion--the IL-17 level, as well as revealing of the inherited genesis of the disorder in a system of fibrillogenesis regulation--the IL-4 level.
Asunto(s)
Hidronefrosis/cirugía , Interleucina-10/sangre , Pelvis Renal/cirugía , Factor de Necrosis Tumoral alfa/sangre , Uréter/cirugía , Adulto , Biomarcadores/sangre , Femenino , Humanos , Hidronefrosis/sangre , Hidronefrosis/congénito , Hidronefrosis/patología , Interleucina-17/sangre , Interleucina-4/sangre , Pelvis Renal/anomalías , Pelvis Renal/metabolismo , Masculino , Pronóstico , Riesgo , Uréter/anomalías , Uréter/metabolismoRESUMEN
The connective tissue metabolism was investigated in patients, suffering hydronephrosis, caused by obstruction of various etiology of pelvio-ureteric segment (PUS) and ureter, which has a recurrent course. On the 21th day postoperatively the blood indices enhancement was revealed, what characterizes the disorder of collagen synthesis and degradation, including, free (FOP), proteinbinded (PRBOP) and peptidebinded (PEBOP) oxyproline. The changes noted are more pronounced in patients with the inborn obstruction of PUS and recurrent course of the disease. A new marker--the PRBOP to FOP levels ratio--was proposed for prognostication of stricture recurrence.
Asunto(s)
Proteínas Sanguíneas/metabolismo , Colágeno/sangre , Tejido Conectivo/metabolismo , Hidronefrosis/sangre , Hidroxiprolina/sangre , Biomarcadores/sangre , Estudios de Casos y Controles , Tejido Conectivo/anomalías , Tejido Conectivo/cirugía , Femenino , Humanos , Hidronefrosis/congénito , Hidronefrosis/patología , Hidronefrosis/cirugía , Pelvis Renal/anomalías , Pelvis Renal/metabolismo , Pelvis Renal/cirugía , Masculino , Unión Proteica , Proteolisis , Uréter/anomalías , Uréter/metabolismo , Uréter/cirugíaRESUMEN
The article deals with the analysis of factors impacting dosage inaccuracy and input of this inaccuracy into total inaccuracy of analysis. The total dosage inaccuracy includes both the value of inaccuracy mentioned in the technical passport of dosage device and inaccuracy values occurring at all stages of dosage during implementation of technique. The characteristics of dosage devices and their merits and shortcomings are taken into account. The article substantiates the choice of types of dosage devices to be implemented in the laboratory research. The modes of errors elimination and inaccuracy minimization were analyzed against the perfectly calibrated and totally corresponded to passport data device of air transfer dosage.
Asunto(s)
Técnicas de Química Analítica/instrumentación , Técnicas de Química Analítica/normas , Técnicas de Laboratorio Clínico/instrumentación , Técnicas de Laboratorio Clínico/normas , Errores Diagnósticos/prevención & control , Análisis de Falla de Equipo/métodos , Calibración , Técnicas de Química Analítica/métodos , Técnicas de Laboratorio Clínico/métodos , Equipos Desechables/normas , Falla de Equipo , Análisis de Falla de Equipo/normas , Gestión de la Calidad TotalRESUMEN
Changes in the immune status of first-form pupils were studied as an child body's adaptation index to schooling conditions. A complex of immunological tests included the following parameters: T lymphocytes (E-RFC), B lymphocytes, T lymphocyte subpopulation, IgA, IgM, IgG, circulating immune complexes (CIC). There was a definite relationship of the schooling conditions to the children's immunity. The routine one-shift schooling regimen in primary-form pupils is not optimal in terms of their adaptation to school, which appears as decreased immunological responsiveness. Full-day schooling of junior schoolchildren allows them to adapt to their new activities more easily after they enter school, the risk for immunological disorders and the likelihood of their related diseases to become lower.
Asunto(s)
Adaptación Fisiológica/inmunología , Sistema Inmunológico/fisiología , Factores de Edad , Complejo Antígeno-Anticuerpo/inmunología , Linfocitos B/inmunología , Niño , Humanos , Inmunoglobulinas/análisis , Formación de Roseta , Linfocitos T/inmunología , Factores de Tiempo , UcraniaRESUMEN
15 women with indeterminate diagnosis of ovarian lesion have undergone a standard examination, NMR tomography and enzyme immunoassay for tumor markers (KA-125, KA-19-9, CEA). NMR-tomography appeared highly informative in differentiating benign and malignant tumors of the ovaries, effective in staging. The oncomarkers are effective in screening for cancer risk groups, control of treatment efficacy and the disease prognosis. Carbohydrate (tumor-associated) antigen KA-125 is most specific for ovarian cancer. Combined use of the tumor markers and NMR-tomography provides a reliable diagnosis.
Asunto(s)
Adenocarcinoma/diagnóstico , Antígeno Ca-125/sangre , Antígeno CA-19-9/sangre , Antígeno Carcinoembrionario/sangre , Imagen por Resonancia Magnética , Neoplasias Ováricas/diagnóstico , Adenocarcinoma/sangre , Biomarcadores de Tumor/sangre , Diagnóstico Diferencial , Femenino , Humanos , Técnicas para Inmunoenzimas , Neoplasias Ováricas/sangre , Sensibilidad y EspecificidadRESUMEN
Studies of daily excretion of hydroxyproline with the urine in patients with chronic pyelonephritis (CP) showed manifest hyper-hydroxyprolinuria at the early stages of the disease, which augmented with the development of renal insufficiency. Measurement of hydroxyproline in the urine of CP patients is proposed as a test reflecting the renal connective tissue metabolism at the early stages of the disease. It can be used for assessing the activity of the sclerotic process in the kidneys of CP patients, for predicting the disease course and outcome, and, probably, for developing methods of adequate pathogenetic therapy based on biochemical monitoring. The method is noninvasive, sufficiently informative, and available for clinical diagnostic laboratories.
Asunto(s)
Hidroxiprolina/orina , Nefroesclerosis/diagnóstico , Pielonefritis/orina , Adolescente , Adulto , Enfermedad Crónica , Ritmo Circadiano , Interpretación Estadística de Datos , Femenino , Humanos , Masculino , Nefroesclerosis/orina , Pronóstico , Programas Informáticos , Espectrofotometría , Factores de TiempoRESUMEN
Blood plasma content was studied of hydrocortisone, parathyroid hormone (PTH) and calcitonin in patients with chronic pyelonephritis (ChP) using methods of radioimmunologic and immunoenzymatic analyses. ChP patients had marked alterations of blood plasma levels of hydrocortisone, PTH and calcytonin, with the reduced levels of hydrocortisone and hypercalcitoninamia being noted during the early stages of nephrosclerosis. It is suggested that hydrocortisone and calcitonin content in blood of ChP patients might be used as indicators of state of the endocrine mechanisms controlling the renal connective tissue metabolism during the early stages of nephrosclerosis. The above measure will also be helpful in the assessment of activity of the sclerotic process in the kidneys, prognosis of the outcome of the medical condition, and development of treatment options of adequate pathogenetic therapy.
Asunto(s)
Calcitonina/sangre , Hidrocortisona/sangre , Fallo Renal Crónico/sangre , Nefroesclerosis/sangre , Hormona Paratiroidea/sangre , Pielonefritis/sangre , Adulto , Enfermedad Crónica , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Cu and Zn levels were measured (nuclear-absorption spectrophotometry) in the blood and urine from patients with chronic pyelonephritis (CP) in emergence of nephrosclerosis and renal insufficiency. CP patients were found to develop hypercupremia as early as initial stages of nephrosclerosis when renal insufficiency was not apparent. Blood Zn was low, especially in development of chronic renal insufficiency. Cu and Zn disbolism may contribute to formation of sclerotic lesions in the kidneys and to development of chronic renal insufficiency. Assay for Cu and Zn is proposed for early diagnosis of nephrosclerosis and provides objective clinical control over the disease course to make timely correction of the detected defects and valid prognosis.
Asunto(s)
Cobre/metabolismo , Fallo Renal Crónico/metabolismo , Nefroesclerosis/metabolismo , Pielonefritis/metabolismo , Zinc/metabolismo , Adulto , Anciano , División Celular , Colágeno/biosíntesis , Cobre/sangre , Cobre/orina , Femenino , Humanos , Riñón/metabolismo , Riñón/patología , Fallo Renal Crónico/diagnóstico , Fallo Renal Crónico/etiología , Masculino , Persona de Mediana Edad , Nefroesclerosis/diagnóstico , Nefroesclerosis/etiología , Pronóstico , Pielonefritis/complicaciones , Pielonefritis/diagnóstico , Espectrofotometría Atómica , Zinc/sangre , Zinc/orinaRESUMEN
Glycosaminoglycanes (GAG) were measured in 24-h urine of patients with chronic pyelonephritis (CP) and chronic glomerulonephritis (CGN) at different stages of nephrosclerosis. These patients developed hyperglycosaminoglycanuria at early stages of nephrosclerosis before establishment of chronic renal failure. There is a positive correlation between the severity of excretion of GAG and leukocyturia in CP patients (p < 0.05). Estimation of GAG 24-h urinary excretion can help evaluate metabolism of connective tissue of the kidneys in CP and CGN patients for early diagnosis of nephrosclerosis, control of the treatment efficacy and the disease prognosis. It can also serve as additional criterion for rating of inflammation. The method is non-invasive, informative, available for clinical diagnostic laboratories.
Asunto(s)
Glomerulonefritis/orina , Glicosaminoglicanos/orina , Pielonefritis/orina , Adulto , Biomarcadores/orina , Enfermedad Crónica , Femenino , Glomerulonefritis/diagnóstico , Humanos , Riñón/metabolismo , Masculino , Persona de Mediana Edad , Pronóstico , Pielonefritis/diagnóstico , Índice de Severidad de la EnfermedadRESUMEN
The time course of daily urinary excretion of hydroxyproline (HOP) was followed up in patients with chronic pyelonephritis. Manifest hyperhydroxyprolinuria was observed in these patients at the earliest stages of nephrosclerosis, and it augmented with development of renal insufficiency. Measurements of HOP in the urine of patients with chronic pyelonephritis is proposed as an indicator reflecting the metabolic status of renal connective tissue at the early stages of nephrosclerosis. It can be used to assess the activity of the sclerotic process in the kidneys of patients with chronic pyelonephritis, predict the disease course and outcome, and, probably, to develop methods of adequate pathogenetic therapy with biochemical monitoring. The method is not invasive, sufficiently informative, and available for clinical diagnostic laboratories.
Asunto(s)
Hidroxiprolina/orina , Nefroesclerosis/diagnóstico , Pielonefritis/patología , Enfermedad Crónica , Ritmo Circadiano , Humanos , Nefroesclerosis/fisiopatología , Nefroesclerosis/orina , Valor Predictivo de las Pruebas , Pielonefritis/fisiopatología , Pielonefritis/orinaRESUMEN
Hydrocortisole, parathyroid hormone (PTH), and calcitonin were radioimmunoassayed in the blood of patients with chronic pyelonephritis. Hydrocortisone content decreased at the early stages of nephrosclerosis before renal failure, and hypercalcitoninemia was developing. In chronic renal insufficiency hydrocortisone level normalized, hypercalcitoninemia augmented, and PTH level in the blood plasma increased. Disorders of these substances metabolism are believed to be one factor in the pathogenesis of pyelonephritic nephrosclerosis. Measurements of blood hydrocortisone and calcitonin in patients with chronic pyelonephritis can be used for early diagnosis of nephrosclerosis before its clinical manifestation as chronic pyelonephritis. Such measurements will help objectively assess the activity of the sclerotic process, timely begin proper treatment, and predict the disease course and outcome.
Asunto(s)
Calcitonina/sangre , Hidrocortisona/sangre , Nefroesclerosis/sangre , Hormona Paratiroidea/sangre , Pielonefritis/sangre , Adulto , Anciano , Biomarcadores , Enfermedad Crónica , Femenino , Humanos , Masculino , Persona de Mediana Edad , Nefroesclerosis/complicaciones , Pielonefritis/complicacionesRESUMEN
Chromatin structural organization was studied by means of electron microscopy in the macronuclei of ciliate Bursaria truncatella at various stages of the life cycle (at different time intervals after cell division, in resting cysts and at excysting) and in the nuclei of myxomycete Physarum polycephalum during the mitotic cycle. Inactive chromatin was shown to be organized in compact clumps 100-300 nm in diameter linked with each other, their loop organization being convincingly demonstrated. Upon activation chromatin decompacts and is represented by nucleosomal fibres with a lot of replicationally and transcriptionally active regions. Both the reptication and transcription processes in physarum nuclei and transcription processes in bursaria can occur on the loops of chromatin fibres emerging from the decompacting clump. The data obtained evidence in favour of a structural-functional correspondence between the chromatin clumps of physarum and bursaria and the chromomeres in chromosomes of higher eucaryotes. Based on the data received it is concluded that the chromatin clump represents a dynamic structure unit able to decompact forming the loop-shaped chromatin fibres. Such a structural organization provides the spacial distribution of DNA in the nuclei and the possibility of selective functioning of definite regions of the genome.
Asunto(s)
Cromatina/ultraestructura , Cilióforos/ultraestructura , Physarum/ultraestructura , Animales , Ciclo Celular , Núcleo Celular/ultraestructura , Cilióforos/fisiología , Microscopía Electrónica , Physarum/fisiología , Conformación ProteicaRESUMEN
The influence of different concentrations of detergent Joy on the chromatin structure of Physarum polycephalum in the process of mitotic cycle was studied electronmicroscopically. The investigations showed that at Joy concentrations less than 0,01% a small part of nuclei disrupt and, as a rule, chromatin is insufficiently dispersed; at concentrations more than 0,1% the detergent may influence the chromatin structure of Physarum polycephalum. Based on the data obtained we consider that the optimal detergent concentrations that practically do not influence the chromatin structure and lead to disruption of the majority of nuclei and to the proper dispersion of chromatin is 0,1-0,01%.