[Is it possible to detect surface antigen CD133 on patient-derived glioblastoma continuous cell cultures using fluorescent aptamers?] / Vozmozhna li detektsiya poverkhnostnogo antigena CD133 na perevivaemykh kul'turakh kletok glioblastomy patsientov s pomoshch'yu fluorestsentnykh aptamerov?

Theranostics combines diagnostics and therapeutic exposure. Regarding glioblastomas, theranostics solves the problem of detecting and destroying tumor stem cells resistant to irradiation and chemotherapy and causing tumor recurrence. Transmembrane surface antigen CD133 is considered as a potential marker of tumor stem cells. OBJECTIVE: To detect CD133 in patient-derived glioblastoma continuous cell cultures using fluorescence microscopy and modified aptamers (molecular recognition elements) anti-CD133. MATERIAL AND METHODS: To detect CD133, we used mousey fluorescence monoclonal antibodies anti-CD133 MA1-219, FAM-modified DNA aptamers anti-CD133 AP-1-M and Cs5. Non-aptamer DNA oligonucleotide NADO was used as a negative control. Detection was performed for three samples of patient-derived glioblastoma continuous cell cultures coded as 1548, 1721 and 1793. RESULTS: MA1-219 antibodies brightly stained cell culture 1548, to a lesser extent - 1721. There was diffuse staining of cell culture 1793. Cs5-FAM aptamer stained cells in a similar way, but much weaker. AP-1-M-FAM aptamer interacted with cells even weaker and diffusely stained only cell culture 1793. Non-aptamer NADO did not stain cell culture 1548 and very weakly diffusely stained cell culture 1793. CONCLUSION: For both molecular recognition elements (MA1-219 antibody and Cs5 aptamer), 3 cell culture samples can be arranged in the following order possibly reflecting CD133 status decrease: strong signal for cell culture 1548, much weaker for 1721, even weaker for 1793. Only cell culture 1548 can be considered CD133 positive with combination of Cs5+ and NADO signals. Cell culture 1793 is CD133 false positive with combination of Cs5+ and NADO+ signals.

[Stage wise treatment of uterine arteriovenous malformation complicated by recurrent haemorrhage]. / Étapnoe lechenie arteriovenoznoi mal'formatsii matki, oslozhnennoi retsidiviruiushchimi krovotecheniiami.

Uterine arteriovenous malformation is a rarely encountered disease threatening with massive haemorrhage. The article describes a clinical case report regarding a 37-year-old woman presenting with this pathology and previously hospitalized twice with severe posthaemorrhagic complications at a 5-month interval due to refusal from timely hysterectomy. A vascular formation in the womb was detected at ultrasonography, however its pattern was identified only by computed tomography of small pelvis organs with intravenous contrasting. However, the complete picture of the architectonics of uterine arteriovenous malformation and extension of the pathology was obtained by selective subtraction angiography, making it possible not only to perform diagnosis but also, if necessary, to immediately perform selective embolization of the supplying vessels. Due to massive uterine bleeding on the background of womb malformation, the woman was twice subjected to roentgenoendovascular embolization of afferent vessels, with the achievement of persistent haemostasis. Hysterectomy was performed after stabilization of the state. Thus, an extensive angiomatous uterine lesion accompanied by recurrent bleedings, along with roentgenoendovascular methods of treatment there is a need of additional surgical resection with the removal of the angiodysplasia focus.

[Production of reactive oxygen species by monocyte-derived macrophages from the blood of healthy donors and patients with IHD].

Production of reactive oxygen species (ROS) by macrophages from blood monocytes of healthy donors (MP(N)) and patients with IHD (MP(IHD)) before, during, and after their incubation with low-density lipoprotein (LDL) isolated from the blood plasma of healthy donors (LDL(N)) and patients with a high cholesterol level (LDL(H)) was estimated by the method of luminol-dependent and stimulated by opsonized zymosan (OZ) or phorbol-12-myristate-13-acetate (PMA) chemiluminescence (CL). Intrinsic luminol-dependent, and zymosan- or PMA-stimulated chemiluminescence of MP(IHD) have exceeded the same types of chemiluminescence of MP(N) by factors of 1.4, 1.8, 2.7, and 1.6, respectively (p < 0.05-0.01). The effect of zymosan on MP(N) and MP(IHD) was stronger than that of TPA by factors of 4.3 and 3.2, respectively, but manifested itself 2.5-3.0 times slower. LDL(N) and LDL(H) incubated with MP(N) during 15-60 min increased ROS production by a factor of 1.4 and 2.5 respectively, but influenced ROS production by MP(IHD) (as estimated by luminol-dependent chemiluminescence). Effects LDL(N) and LDL(H) on MP(IHD) were not detected at all. Repeated increase in zymosan-stimulated CL of MP(N) was also observed after their 15-180 min preincubation with LDL(N) and LDL(H) which followed after taking out LDL, washing MP(N) and adding Hanks' solution with opsonized zymosan. This increase was also stronger after MP(N) incubation with LDL(H) than after MP(N) incubation with LDL(N), and no increase was observed in experiments with MP(IHD). Thus, the results obtained by a chemiluminescent method showed that fresh macrophages from the blood of patients with IHD had higher ROS production than macrophages from healthy donors. LDL(N) and LDL(H) could exhibit primary and secondary (after preincubation) stimulating effect on CL in MP(N); but had no effect on MP(IHD). An analysis of macrophage chemiluminescence is a sensitive test for evaluation the degree of macrophage's stimulation and it may be effectively used for the dynamic control for treatment effectiveness in clinics.

[The use of antioxidants and antihypoxants for decreasing of LDL oxidation by macrophages and endothelial cells in ischemia and reperfusion of vascular wall]. / Primenenie antioksidantov i antigipoksantov dlia snizheniia okisleniia LNP pod vliianiem makrofagov i éndotelial'nykh kletok v usloviiakh ishemii i reperfuzii sosudistoi stenki.

Oxidative modification of LDL is a key factor in pathogenesis of atheroslerosis. In this work the effects of antioxidants (K-phenosan, probucol, and desferal) and antihypoxants (succinic acid, hypoxen, and deltaran) on the macrophage- and endothelial cell-mediated oxidation of LDL was studied. Electrophoretic mobility of LDL, the content of lipid peroxide products (TBARS and diene conjugates, DC) and cell viability were used as the indexes of LDL oxidation. The effectiveness of antioxidants as inhibitors of LDL oxidation decreased in the following order: desferal > probucol > K-phenosan, and antihypoxant ability was decreased in line: deltaran >> succinic acid (effect only in dose 40 mg/ml) >> hypoxane (no effect). The effect antioxidants on protection of cell viability (MP and EC) during ischemia reduced in the same order. The effectiveness of antihypoxant protection of MP viability, decreased in the following order: succinic acid > hypoxen >> deltaran. Macrophages added to 24 h ischemized EC + LDL in early reperfusion period decreased LDL EPM. This may apparently be attributed to selective uptake of oxidized LDL by MP.

[Physico-chemical properties of water-soluble proteins from Spirulina platensis]. / Issledovanie nekotorykh fiziko-khimicheskikh svoistv vodorastvorimykh belkov Spriulina platensis.

By gel chromatography and polyacrylamide gel electrophoresis it was demonstrated that water-soluble proteins from Spirulina platensis contained components differing in their structure and having molecular weights ranging from 300,000+/-30,000 to 11,000 +/- 1,000. Electrophorectically the proteins were heterogeneous and their individual fractions differed in the number of pigment and protein components. The pigment-protein components were complexes in which the pigment was strongly bound to the protein. The protein components could be in a free state and in a pigment-bound state.