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1.
Nutr Metab Cardiovasc Dis ; 25(12): 1152-8, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26610654

RESUMEN

BACKGROUND AND AIM: Mitochondrial dysfunction contributes to the pathophysiology of diabetic cardiomyopathy. The aim of this study was to investigate the acute changes in the mitochondrial proteome in response to insulin stimulation. METHODS AND RESULTS: Cardiac mitochondria from C57BL/6 mice after insulin stimulation were analyzed using two-dimensional fluorescence difference gel electrophoresis. MALDI-TOF MS/MS was utilized to identify differences. Two enzymes involved in metabolism and four structural proteins were identified. Succinyl-CoA ligase [ADP forming] subunit beta was identified as one of the differentially regulated proteins. Upon insulin stimulation, a relatively more acidic isoform of this protein was increased by 53% and its functional activity was decreased by ∼32%. CONCLUSIONS: This proteomic remodeling in response to insulin stimulation may play an important role in the normal and diabetic heart.


Asunto(s)
Acilcoenzima A/efectos de los fármacos , Cardiomiopatías Diabéticas/fisiopatología , Insulina/farmacología , Mitocondrias Cardíacas/efectos de los fármacos , Mitocondrias Cardíacas/fisiología , Proteoma/efectos de los fármacos , Acilcoenzima A/metabolismo , Animales , Western Blotting , Cardiomiopatías Diabéticas/metabolismo , Modelos Animales de Enfermedad , Electroforesis en Gel Bidimensional/métodos , Inyecciones Intraperitoneales , Ratones , Ratones Endogámicos C57BL , Distribución Aleatoria , Sensibilidad y Especificidad , Espectrometría de Masas en Tándem/métodos , Recolección de Tejidos y Órganos , Remodelación Ventricular/fisiología
2.
Can Med Assoc J ; 126(10): 1149, 1982 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-20313743
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