Variation in predator response to short-wavelength warning coloration.

Warning coloration deters predators from attacking unpalatable prey, and is often characterized by long-wavelength colors, such as orange and red. However, warning colors in nature are more diverse and include short-wavelength colors, like blue. Blue has evolved as a primary defense in some animals but is not common. One hypothesis for the maintenance of this diversity is interspecific variation in predator responses to signals. We tested this hypothesis with galliform birds: Gambel's quail (Callipepla gambelii) and two domestic chicken breeds (Gallus gallus domesticus; Plymouth Rocks, Cochin Bantams). We measured innate avoidance and learning responses to only blue prey, only orange prey, and orange-and-blue prey, where the blue was iridescent to represent the natural coloration of the pipevine swallowtail butterfly (Battus philenor). We predicted birds would have similar responses to orange, but vary in response to blue. Upon first encounter, Cochin Bantams did not attack blue and Gambel's quail readily attacked, indicating innate avoidance by Cochin Bantams. Plymouth Rocks had no innate aversion to any color, lower attack latencies and attacked most prey items. Cochin Bantams and Gambel's quail both learned orange and orange-and-blue quicker than blue. Our results support the hypothesis that interspecific variation in predator response could maintain warning color diversity.

Male wing color properties predict the size of nuptial gifts given during mating in the Pipevine Swallowtail butterfly (Battus philenor).

In many animals, males bear bright ornamental color patches that may signal both the direct and indirect benefits that a female might accrue from mating with him. Here we test whether male coloration in the Pipevine Swallowtail butterfly, Battus philenor, predicts two potential direct benefits for females: brief copulation duration and the quantity of materials the male passes to the female during mating. In this species, males have a bright iridescent blue field on the dorsal hindwing surface, while females have little or no dorsal iridescence. Females preferentially mate with males who display a bright and highly chromatic blue field on their dorsal hindwing. In this study, we show that the chroma of the blue field on the male dorsal hindwing and male body size (forewing length) significantly predict the mass of material or spermatophore that a male forms within the female's copulatory sac during mating. We also found that spermatophore mass correlated negatively with copulation duration, but that color variables did not significantly predict this potential direct benefit. These results suggest that females may enhance the material benefits they receive during mating by mating with males based on the coloration of their dorsal hindwing.

Warning color changes in response to food deprivation in the pipevine swallowtail butterfly, Battus philenor.

Predation on distasteful animals should favor warning coloration that is relatively conspicuous and phenotypically invariable. However, even among similarly colored individuals there can be variation in their warning signals. In butterflies, individual differences in larval feeding history could cause this variation. The warning signal of the pipevine swallowtail butterfly, Battus philenor L. (Lepidoptera: Papilionidae) consists of both a blue iridescent patch and pigmentbased orange spots on the ventral hindwing. B. philenor males also display a dorsal surface iridescent patch that functions as a sexual indicator signal. A previous study of iridescence in B. philenor found that the iridescent blue on both the dorsal and ventral hind wings is variable and significantly different between lab-reared and field-caught individuals. These differences could be the result of larval food deprivation in the field. Through experimental manipulation of larval diet, larval food deprivation was evaluated as a potential cause of the differences observed between lab and field individuals, and if food deprivation is a source of inter-individual variation in warning signals. B. philenor larvae were food restricted starting at two points in the last larval instar, and one group was fed through pupation. Adult coloration was then compared. Food deprivation led to poorer adult condition, as indicated by lower adult body mass, forewing length, and fat content of stressed individuals. As the level of food deprivation increased, the hue of the iridescent patches on both the dorsal and ventral hind wing shifted to shorter wavelengths, and the chroma of the orange spots decreased. The shifts in iridescent color did not match the differences previously found between lab and field individuals. However, the treatment differences indicate that food deprivation may be a significant source of warning color variation. The differences between the treatment groups are likely detectable by predators, but the effect of the variation on signal effectiveness and function remains to be empirically explored.