Derivation of multivariate indices of milk composition, coagulation properties, and curd yield in Manchega dairy sheep.

This study approaches the interrelation patterns between composition of milk and whey, curd yield, chromaticity, syneresis, and technological quality of Manchega sheep milk using multivariate factor analysis. In addition, the effect of the main husbandry components (flock, prolificacy, season of the year, stage of lactation, and parity) on the common latent factors that define the pattern of variation of Manchega milk was assessed. For this purpose, 1,200 individual Manchega ewe milk samples from 4 different flocks registered under the Protected Designation of Origin Queso Manchego were analyzed (50 ewes/flock). Samples were collected in 2 different seasons of the year (spring and autumn) and at 3 time points per season: early, mid-, and late lactation. The obtained results suggested that curd yield mainly depends on milk composition, and the retention of water in the curd is related to coagulation traits. Thus, composition and moisture content could be useful indicators to assess the efficiency and quality of milk intended for cheesemaking, regardless of the analysis of coagulation properties. Finally, in terms of husbandry, a direct effect of flock and stage of lactation was observed on all analyzed factors, with a lower influence of season and parity.

Using multivariate analysis to explore the relationships between color, composition, hygienic quality, and coagulation of milk from Manchega sheep.

This study explores the relationships between composition, hygienic quality, and color values of milk to evaluate whether colorimetry could be used as a valid predictor of the quality of raw milk and its coagulation. For this purpose, we performed analyses in 1,200 individual samples of Manchega sheep milk from 4 flocks from the region of Castilla-La Mancha, Spain. Based on the measured variables, we determined the differences and similarities between coagulating and noncoagulating samples using discriminant analysis techniques. The variables with a higher discriminant ability were lactose content, somatic cell score, pH, and the color values lightness (L*) and red/green value (a*). The model based on color values showed a predictive ability similar to that found in the model based on milk composition and hygienic quality. Canonical correlation analysis allowed us to explore the relationships between both sets of variables. Canonical correlations for the first and second pair of canonical values were 0.794 and 0.438, respectively. Both values were significant and represented 92.82% of the observed variability. The correlation structure showed that color values had a strong correlation with fat and protein content and with total solids, and they had a weak correlation with lactose content and somatic cell score. The 2 first combinations of standardized canonical variability could be considered a predictable measure of the composition and, to a lesser extent, the hygiene of milk. Measurement of color values could be a rapid and effective means of supplementing standard analyses when determining the coagulation ability of Manchega sheep milk.

Multivariate analysis of the milk coagulation process in ovine breeds from Spain.

In Spain, ewe milk is mainly used for cheesemaking, and farming systems have traditionally been based on the use of autochthonous breeds. However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude. The purpose of this work was to explore milk composition and coagulation to classify 4 of the main dairy sheep breeds used in Spain. This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches. Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield. An indicator of coagulation efficiency was also determined. Canonical discriminant analysis was performed to establish differences and similarities among breeds based on the measured variables. In addition, cluster analysis was performed to study and quantify the concrete relationships among the discriminated groups. Discriminant analysis proved to be a powerful tool to accurately draw distinctions between breeds. In all cases, discrimination among breeds was evident and the 4 breeds could be easily differentiated. Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition. However, Merino de Grazalema and Manchega were difficult to separate according to milk composition, but the coagulation process differenced them clearly. Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.

Coagulation efficiency and its determinant factors: A case study for Manchega ewe milk in the region of Castilla-La Mancha, Spain.

Milk coagulation, especially in small ruminant species, is often hard to evaluate, as coagulation traits are normally considered individually and several factors related to udder health might distort yield calculation. Due to the lack of studies about these factors, our objective was to determine milk coagulation efficiency (CE) and its determinants using a deterministic technical efficiency approach, an ordinary least square regression model, and ANOVA. Milk from 300 Manchega ewes was collected and analyzed for composition, milk coagulation properties, and hygienic quality. The study results indicate that the estimated CE in Manchega ewes was 0.69, implying an important proportion of the animals produce poorly coagulating milk. The results of the ordinary least square regression model and ANOVA revealed that the main factor causing inefficiency was the initial pH of milk. Crude protein, casein and plasmin activity had moderate effects on CE, and, finally, other factors such as freezing point depression, somatic cell count, colony-forming units, and fat concentration had minor effects.

Selenium status in a group of schoolchildren from the region of Madrid, Spain.

BACKGROUND: To assess the selenium status of a group of schoolchildren from the Region of Madrid, Spain. METHODS: Study subjects comprised 483 children (216 boys and 267 girls) aged between 8 and 13 years. Selenium intake was determined using a 3-day food record. The foods consumed were transformed into energy and nutrients, and the selenium intake was compared with that recommended. Serum selenium levels were also recorded. RESULTS: Mean (SD) selenium intake [91.0 (25.2) µg day(-1) ] was above the recommended level in 99.4% of subjects; the main dietary sources were cereals, meats, fish and milk products. The serum selenium concentration [mean (SD) 71.1 (14.4) µg L(-1) ], however, was <60 µg L(-1) in 13.9% of subjects, and <45 µg L(-1) in 5.6%. The serum selenium concentration correlated with the selenium intake (r = 0.169; P < 0.05). Children with a serum selenium concentration of <75 µg L(-1) had significantly smaller selenium intakes than those with a serum concentration of ≥75 µg L(-1) . CONCLUSIONS: Although selenium intake was generally above that recommended, the serum selenium concentration of the children could be improved. This could be achieved by increasing the relative consumption of cereals and other selenium rich foods such as fish.

[Folate status in Spanish schoolchildren and its association with parental smoking habits]. / Situación de folatos en escolares españoles y su asociación con la presencia del hábito tabáquico en los padres.

AIM: To study the folate status in a group of Spanish schoolchildren and its association with parental smoking habits. METHODS: A group of 511 schoolchildren between 9 and 12 years old from Madrid (Spain) were studied. Parental smoking habits were self-reported by a questionnaire. Energy and nutrients intake (especially folates) were calculated with a "3-day diet record" and during two days (Monday and Tuesday) the food consumption was controlled in the school canteen using the "precise weighing method" (recording the weights of food served and leftovers on the plate). Folate intake of the sample was compared with the recommended intakes (RI) for this vitamin. Folate, vitamin B12 and homocysteine were determined. Student's t-test, Mann Whitney Test, Pearson and Spearman correlation, ANCOVA, MANCOVA and multiple linear regression were applied for statistical analysis. Significance was set at P < 0.05. RESULTS: Vegetable and fruit consumption, folate intake and its coverage to recommended folate intake (I/RI%) and serum folate levels were higher in children of nonsmoking mothers than in children of smoking mothers. Serum folate level in 13.3% of the children studied was below 6 ng/mL (moderate deficit), and in 0.26% was below 3 ng/mL (severe deficit). Taking into account others confounding factors a negative and significant correlation was observed between serum folate levels of children and smoking habits in mothers (r = -0.257, p = 0.01). CONCLUSION: The maternal smoking habits might determine folate intake and serum folate levels on their offspring.

Influence of the consumption of fruits and vegetables on the nutritional status of a group of institutionalized elderly persons in the Madrid region.

AIM: To determine the nutritional status in a group of institutionalized elderly people in the Madrid region of Spain, with respect to their consumption of fruits and vegetables. SETTING: The Madrid region, Spain. PARTICIPANTS: Men and women 65 years of age (n=180). MEASUREMENTS: A dietetic study undertaken using the precise weighing method (7 consecutive days). Anthropometric and blood analysis data were also recorded. The study subjects were grouped according to whether or not they consumed the WHO/FAO-recommended amount of 400 g/day of fruits and vegetables. RESULTS: Mean fruit and vegetable consumption was 446.77 ± 168.80 g/day. The subjects who consumed at least 400 g/day of these foods also took in the largest total weight of food, and had the largest macronutrient, fiber, vitamin and mineral intakes. They also had higher serum and erythrocyte folate concentrations and lower plasma homocysteine concentrations than those who consumed less than the recommended 400 g/day of fruits and vegetables. CONCLUSIONS: The subjects who consumed at least 400 g/day of fruits and vegetables generally showed a better nutritional status. It would be advisable that the consumption of such foods be relatively increased, especially by those who currently consume less than 400 g/day.

Fat intake and asthma in Spanish schoolchildren.

OBJECTIVES: To study the relationship between lipid, fatty acid and lipid-rich food intake and current asthma in a group of Spanish schoolchildren. SUBJECTS/METHODS: The subjects of this cross-sectional study were 638 Spanish schoolchildren (8-13 years of age). The weight and height of all the subjects were recorded. A questionnaire, completed by the subjects' parents, was used to obtain personal and health information. Current asthma was established when the children had ever had asthma, if they had been diagnosed with asthma by a doctor and if they had been treated with medications at some time in the previous 12 months. Food intake was monitored using a 3-day food record. All foods consumed were converted into energy and nutrients. RESULTS: The energy derived from lipids, saturated fatty acids (SFAs) and myristic and palmitic acids was independently associated with current asthma (Odds Ratio (OR) third tertile 2.85 (1.01-8.07) P=0.049, 10.00 (0.89-111.97) P=0.002, 11.21 (1.36-92.24) P=0.002, 7.58 (1.40-41.03) P=0.022, respectively), as was the intake of butter (OR third tertile 2.97 (1.01-8.68) P=0.001). No relationship was seen between this condition and the intake of any other fatty acid, the n-6/n-3 ratio, nor the consumption of margarine, milk products, fish, meat, eggs or vegetable oils. CONCLUSIONS: Increased intakes of SFAs, myristic and palmitic acids and butter seem to be related to the risk of current asthma in children.

[Obesity, insulin resistance and increase in adipokines levels: importance of the diet and physical activity]. / Obesidad, resistencia a la insulina y aumento de los niveles de adipoquinas: importancia de la dieta y el ejercicio físico.

With obesity the amount of pro- and anti-inflammatory adipokines released is increased within the fat tissue. These molecules are implicated in many clinical manifestations of this pathology such as diabetes, arterial hypertension, or cardiovascular disease. At the first stage, the fat tissue of the obese patient becomes resistant to the action of insulin due to the effect of some of these adipokines such as tumour necrosis alpha (TNF-alpha) or interleukine-6 (IL-6). At a second stage, this resistance occurs at other tissues and glucose and insulin levels are increased. This increase, together with high adipokines levels that occur in diabetes, lead to the occurrence of different adverse events, such as the following: increase in oxidative stress, endothelial dysfunction, increase in blood pressure, and impairments in lipoprotein metabolism, all of which are harmful for health. Practising physical activity and following a balanced diet, similar to the Mediterranean pattern with low fat and salt consumption, would be helpful for improving insulin resistance and adipokines levels in obese people, thus helping improving their health status in the long run.

[Mother's age as a conditioning factor of food consumption and energy and nutrients intake of their offspring at pre-school age]. / La edad de la madre como condicionante del consumo de alimentos y la ingesta de energía y nutrientes de sus hijos en edad preescolar.

OBJECTIVES: The aim of this study was to analyse whether the mother's age at the offspring's birth may condition food consumption and energy and nutrients intake as well as the appropriateness of the diet of their offspring at pre-school age. MATERIALS AND METHODS: socio-economic, anthropometrical, and dietary data were collected from a group of 103 pre-school children from Madrid, and the population was divided according to the mother's age (= 26 years (percentile 25) or < 26 years) at birth. RESULTS: Twenty-two point five of the mothers of the preschool children studied had their offspring before 26 years of age and 6.8% before 18. Children from younger mothers consumed less sugars (p < 0.01) and alcohol-free beverages (p < 0.05), but also less fruits (p < 0.01) and others (p < 0.05). The energy from fats was lower (p < 0.05) and that from carbohydrates higher (p < 0.05) among children with older mothers. Besides, the lipid profile was also better in this children, with less caloric intake from polyunsaturated fats (p < 0.05). Besides, fibre (p < 0.05) and vitamin C (p < 0.001) intake was higher in children from older mothers. CONCLUSIONS: It seems that mother's age may have an influence on dietary habits of their children and be a protection factor against dietary inappropriateness of the offspring, so that younger mothers might need special counselling in this respect.

Vulvar leiomyosarcoma in Bartholin's gland area: a case report and literature review.

BACKGROUND: Malignant tumours of the vulvar soft tissue are very uncommon. When localized in the Bartholin's gland area these tumours can be mistaken for benign lesions, leading to a delayed diagnosis. CASE: A 52-year-old woman presenting with a vulvar nodule, which was diagnosed as a Bartholin's gland cyst and was referred to the hospital for surgical excision of the lesion. Pathologist report informed of a 6 cm diameter leiomyosarcoma of the vulva with compromised resection margins; extension studies did not suggest any additional lesions and radical hemivulvectomy with ipsilateral inguinal lymphadenectomy was performed. The patient subsequently received radiotherapy and chemotherapy. Twelve months later, a local recurrence was diagnosed and was removed surgically. After 4 years of follow-up the patient remains disease free. CONCLUSION: Any vulvar lesion with unusual characteristics or insidious evolution in labia majora or Bartholin's glands area should be carefully and promptly studied. This is particularly important in order to perform an effective surgical treatment in cases of leiomyosarcoma.

[Dietary habits and their relationship with the knowledge on the concept of a balanced diet in a group of young women with overweight/obesity]. / Hábitos alimentarios y su relación con los conocimientos, respecto al concepto de dieta equilibrada, de un colectivo de mujeres jóvenes con sobrepeso/obesidad.

OBJECTIVE: To know the dietary habits of a population of women with overweight/obesity and their knowledge on the concept of proper feeding in order to design nutrition educational campaigns focused on this population. MATERIALS AND METHODS: A group of 67 women, aged 20 to 35 years with body mass index 24-35 kg/m2 were asked about their frequency of food intake and what they thought to be correct to achieve a balanced diet. RESULTS: Studied women more frequently consumed oils (4.3 +/- 4.5 times/week) and sweets (2.5 +/- 3.6 times/week) and less frequently water (-19.1 +/- 22.7 times/week), vegetables (-4.3 +/- 5.7 times/week), fruits (-3.7 +/- 6.2 times/week), legumes (-1.3 +/- 2.0 times/week) and fish (-2.2 +/- 3.7 times/week) than what they thought they should consume. When comparing what they declare to consume to what has been established as minimal recommended intake for the different groups of foods it is observed that the intake of grains and legumes, fruits and vegetables, and slightly less that of fish and eggs, should de increased. CONCLUSIONS: given the incorrect dietary habits observed in a population of women with overweight/obesity, it should be convenient to carry out nutrition educational campaigns. This education could help improve the diet of all the population and the occurrence of overweight and obesity could be prevented in many cases. Besides, in those cases in which the pathology is already present, this could prevent following regimens that are harmful for the health and for weight management.

Laboratory rearing conditions for improved growth of juvenile Helix aspersa Müller snails.

A laboratory rearing system in semi-controlled conditions is proposed to facilitate the behavioural rhythms of the edible snail (Helix aspersa) and to produce a high growth rate with low variability. The growth data were used to construct a model for weight estimation based on age. The animals' live weights showed low variability (<17%) and normal distribution. The best model for estimating weight from age is the logistic model, with a high corelation coefficient (>90%), and a high level of significance for the coefficient (P < 0.0001).

Influence of the desire to lose weight on food habits, and knowledge of the characteristics of a balanced diet, in a group of Madrid university students.

OBJECTIVE: The aim of this study was to determine the desire to lose weight in a group of university students, and to analyse the influence of this desire on declared food habits. The degree of knowledge on what constitutes a balanced diet was also determined. DESIGN: Cross-sectional population survey. SUBJECTS: The study subjects were 234 university students (48 males and 186 females) aged 22.3+/-1.5 y. The weight and height of each were recorded. All subjects were questioned on their desire to lose weight, habitual food intake frequencies, and about what they considered to constitute a balanced diet. RESULTS: Approximately half the population (47.9%), both male (47.8%) and female (47.9%), expressed a desire to lose weight, even though the percentage of overweight students was low (11.1% overall, although much greater among male students [39.6%] than female students [3.8]). The body mass index (BMI) of those who wanted to lose weight was greater than that of those who did not. Nonetheless, the majority of subjects (especially females) showed BMIs within the normal range (18-25 kg/m(2)). Among those who wished to lose weight, the consumption of sweet foods was lower - although these subjects believed they ought to consume even less. It was also thought necessary that egg consumption be reduced, although no differences were recorded in declared consumption between those who wished to lose weight and those who did not. CONCLUSIONS: The results show that there is great concern over body weight, although no great differences were seen in the food habits and nutrition knowledge of those who wished to lose weight and those who did not.

Metástasis miocárdica de un carcinoma epidermoide de cérvix en una mujer con trasplante renal / Myocardium metastases from an epidermoid carcinoma of the cervix in a woman with renal transplant

Propósito: La afectación metastásica cardíaca del carcinoma epidermoide de cérvix es muy rara; cuando se presenta suele ser en casos con diseminación generalizada, descubierta en la autopsia. Hay muy pocos casos descritos de metástasis cardíaca secundaria a un carcinoma escamoso de cervix, diagnosticada "in vivo" sin que exista recidiva local o evidencia de enfermedad a distancia y ningún caso descrito en mujer inmunocomprometida. Material y método: Presentamos el caso de una paciente de 54 años portadora de un trasplante renal, sometida a terapia inmunosupresora, que recibió tratamiento exitoso para un cáncer escamoso de cérvix, estadio IIB, a la que se diagnosticó una metástasos cardíaca de gran agresividad. Se describen los hallazgos histopatológicos y revisa la bibliografía existente. Conclusiones: El curso de las enfermedades tumorales en pacientes trasplantados es más agresivo que el esperado en los no transplantados, siendo muy importante intensificar el seguimiento de los supervivientes sometidos a tratamiento inmunosupresor para detectar los tumores malignos tan pronto como sea posible (AU)

Influence of maternal education on food consumption and energy and nutrient intake in a group of pre-school children from Madrid.

A study was conducted on the influence of maternal education level on food consumption, energy and nutrient intake, and dietary adequacy in 110 pre-school children from Madrid, Spain. With increasing maternal education, children consumed more sugar(p < 0.05), fruit (p < 0.05), and fish (p < 0.05). Snacking was more frequent with decreasing maternal education (p < 0.05). Though statistical significance was not reached, the consumption of pre-cooked foods was greater among children of mothers educated to a higher level, a phenomenon probably related to the work situation of these women. With respect to dietary composition, no significant differences were found between groups for macronutrient, fiber and energy intakes, except for energy supplied by polyunsaturated fatty acids (PUFA), which was greater in the children of less educated women (p < 0.01). This is probably due to their greater consumption of sunflower seed oil. The diets of children belonging to well-educated mothers came closer to meeting the recommended intakes for folate, vitamin C, and iodine. It would seem that maternal educational level influences the food habits of children. Mothers with less education may require special advice in this area.