[The use of monolaurates for removing endotoxins from preparations of cell-free pertussis vaccine]. / Ispol'zovanie monolauratov dlia udaleniia éndotoksinov iz preparatov beskletochnoi kokliushnoi vaktsiny.

The possibility of using monolaurates for removing endotoxin from acellular pertussis vaccines developed at the Laboratory of Immunomodulators, Mechnikov Research Institute for Vaccines and Sera (Moscow), has been studied. Monolaurates PEG-400 and PEG-600 obtained, respectively, from Fluka Chemie AG and Ferak GmbH (Germany) have been used. The use of monolaurate PEG-600 ensures the decrease of the toxicity of acellular pertussis vaccines by 2.34-6.3 times.

[The choice of a method of isolating human IgG F(ab)2 fragments for determining antibodies to staphylococcal protein A]. / Vybor metoda polucheniia F(ab)2-fragmentov IgG cheloveka dlia opredeleniia antitel k stafilokokkovomu proteinu A.

The comparative study of two methods of the proteolysis of IgG with the aim of obtaining F(Ab)2-fragments from the blood sera of patients and healthy donors has been made. Different methods for the isolation of F (ab)2-fragments, such as gel filtration on Sephadex G-200, affinity chromatography on protein A-BrCN-sepharose, reprecipitation with zinc sulfate, have been analyzed. For further work the method of the peptic decomposition of whole serum with subsequent salting out with ammonium sulfate and gel filtration on Sephadex G-200 has been chosen.

[The growth and protein A accumulation of Staphylococcus aureus A-676 on a medium made from nonfood raw material]. / Rost i nakoplenie proteina A Staphylococcus aureus A-676 na srede iz nepishchevogo syr'ia.

In this research the experimental analytical method of balancing culture media, based on the determination of the yield of the target product, i.e. staphylococcal protein A, has been used. The medium, developed in the course of this research work on the basis of a hydrolysate of protein-containing waste products of the tanning industry, makes it possible to achieve the growth of producer strains similar to that achieved in peptone-yeast medium. The parameters of the growth of S. aureus strain A-676 and the biological activity of protein A obtained in the newly developed medium have been studied.

[Determination of ovalbumin-specific IgG antibodies in mouse sera by immunoenzyme analysis using horseradish peroxidase-conjugated staphylococcal protein A]. / Opredelenie spetsificheskikh IgG-antitel k oval'buminu v syvorotkakh myshei metodom immunofermentnogo analiza s ispol'zovaniem stafilokokkovogo proteina A, kon''iugirovannogo s peroksidazoi khrena.

The article deals with the possibility of using staphylococcal protein A conjugated with horse-radish peroxidase for the detection of specific IgG-antibodies to ovalbumin in mice by the indirect and competitive EIA techniques. Studies on specifying the parameters of the EIA system for the detection of specific IgG-antibodies are in progress.

[Immunoenzyme test system for the quantitative determination of tetanus antitoxin in vaccinated persons using horseradish peroxidase-conjugated staphylococcal protein A]. / Immunofermentnaia test-sistema dlia kolichestvennogo opredeleniia protivostolbniachnogo antitoksina u vaktsinirovannykh s ispol'zovaniem stafilokokkovogo proteina A, kon''iugirovannogo s peroksidazoi khrena.

An enzyme immunoassay (EIA) system for assays of tetanus antitoxin in vaccinees has been developed. As conjugate, staphylococcal protein A labeled with horse-radish peroxidase is used in this system. The possibility of using the newly developed EIA system in seroepidemiological surveys of the population is shown.

[Possibility of inactivation by formalin of staphylococcal protein A at different stages of its production]. / Izuchenie vozmozhnosti inaktivatsii formalinom materiala, soderzhashchego stafilokokkovyi protein A, na raznykh étapakh ego polucheniia.

The possibility of the formalin inactivation of material containing staphylococcal protein A in the process of obtaining the preparation has been studied. The inactivation of the material with formalin at a concentration of 5% (from the volume of the material) for 1 hour has been shown to ensure a complete bacteriostatic effect and the stability of the initial biological activity of staphylococcal protein A.

[Choice of the method for isolating conjugates of staphylococcal protein A with peroxidase for immunoenzyme analysis]. / Vybor metoda polucheniia kon"iugatov stafilokokkovogo belka A s peroksidazoi dlia immunofermentnogo analiza.

An attempt to obtain the preparations of peroxidase-labeled staphylococcal protein A, intended for use in the enzyme immunoassay, by the glutaraldehyde method has failed. The modified periodate method permitting the preparation of active conjugates of staphylococcal protein A with peroxidase has been developed.

[Determination of protein A I staphylococcus aureus strains isolated from monkeys]. / Opredelenei belka A u shtammov Staphylococcus aureus, vydelennykh ot obez'ian.

Protein A content in Staphylococcus aureus isolated from 6 species of monkeys at the Sukhumi Monkey Nursery has been studied. Protein A has been detected in 73% of the studied strains. One strain isolated from a rhesus macaque has been found to release high amounts of protein A into the environment.

[Optimal conditions for culturing Staphylococcus aureus strain A-676 to obtain biologically active protein A]. / Izuchenie optimal'nykh uslovii kul'tivirovaniia shtamma Staphylococcus aureus A-676 s tsel'iu polucheniia biologicheski aktivnogo proteina A.

Peptone-yeast and casein-salt culture media have been shown to be equally effective when used for the cultivation of S. aureus strain A-676 with a view to obtaining protein A. The seed dose has been determined and the optimum conditions for growing the producer strain have been established.

[Dynamics of staphylococcal infection in guinea pigs with delayed hypersensitivity to Staphylococcus aureus surface antigens]. / Dinamika stafilokokkovoi infektsii u morskikh svinok na fone povychennoi chuvstvetel'nosti zamedlennogo tipa k poverkhnostnym antigenam Staphylococcus aureus.

The relationship between delayed hypersensitivity (DH) to S. aureus surface antigens and the intensity of the infectious process induced by the sublethal infection of guinea pigs with S. aureus was studied. The protective effect, manifested by a decrease in the staphylococcal contamination of the spleen tissue and by an increase in the level of the activation of lymphocytes, was shown to correlate with DH induced by inactivated staphylococcal cells. In infected guinea pigs having DH to different staphylococcal antigens the disease either took a more severe course (in cases of DH to cell wall or peptidoglycan) than in the animals subjected only to infection, or no aggravation of the disease was observed (in cases of DH to protein A).

[Staphylococcal infection in guinea pigs with enhanced delayed hypersensitivity induced by Staphylococcus aureus surface antigens]. / Stafilokokkovaia infektsiia u morskikh svinok na fone povyshennoi chuvstvitel'nosti zamedlennogo tipa, indutsirovannoi poverkhnostnymi antigenami Staphylococcus areus.

A comparative study was made of the relationship between the DHS phenomenon and certain staphylococcal antigens and the intensity of infectious process in sublethal infection of guinea-pigs by Staphylococcus aureus. The protective effect, which was manifested by reduced amounts of staphylococcal colonies in spleen tissues and elevated level of lymphocytes, and neutrophil activation, correlated with DHS induced by surface antigens under study (cell wall, peptidoglycan, protein A). Incomplete combination or certain antigens induced DHS but did not increase the resistance to the following infection.

[Use of different media for growing the producer of the restriction enzyme XBA I]. / Ispol'zovanie razlichnykh sred dlia vyrashchivaniia produtsenta restriktiruiushchego fermenta XBA I.

The possibility of using culture media prepared from local ingredients and intended for growing the producers of restricting enzyme Xba 1 has been demonstrated. The yield of restricting enzyme Xba 1 per g of crude biomass, obtained with the use of peptone-yeast medium prepared from ingredients supplied by Difco Laboratories (USA), has proved to be 4 times greater than that obtained with the use of peptone-yeast medium prepared from local ingredients. At the same time the use of casein-saline medium ensures the yield of the enzyme, similar to that obtained with the use of peptone-yeast medium prepared from ingredients supplied by Difco Laboratories, but with a greater content of nonspecific nucleases.

[Electron microscopic study of microorganisms grown on nutrient media with a nonnutrient raw material base]. / Elektronno-mikroskopicheskoe izuchenie nekotorykh mikroorganizmov, vyrashchennykh na pitatel'nykh sredakh s osnovoi iz nepishchevogo syr'ia.

A comparative electron-microscopic study of Sh. flexneri 8512, Str. Dick Ia and Cor. pseudodiphthericum test strains, grown in experimental culture media prepared from inedible raw materials and in currently used culture media based on fish and meat, has been carried out. The ultrastructure of the test strains grown in culture media prepared from inedible raw materials has been shown to remain unchanged.

[Prospects for the use of blood substitutes made in the USSR with the expired date of validity]. / Perspektivy ispol'zovaniia krovezamenitelei otechestvennogo proizvodstva s istekshim srokom godnosti.

As revealed, blood substitutes--aminopeptide, caseine hydrolysate, and hydrolysine with the expired date of validity could be used as the basis of nutrient media for the cultivation of bacteria. Bacterial growth proved to be the optimum in addition to the blood substitutes of a 0.25--0.5% of yeast extract.

[Change in the protective activity of the ribosomal fractions of Salmonella typhimurium in mutations in the rfa region and the transduction replacement of the rfb region]. / Izmenenie protektivnoi aktivnosti ribosomal'nykh fraktsii Salmonella typhimurium pri mutatsiiakh v oblasti rfa i transduktsionnom zameshchenii oblasti rfb.

It was shown that mutation in the rfa region causing disturbances in the structure of the basal part of the polysaccharide of the cell wall or the absence of O-specific side chains led to the loss of protective activity of the ribosomal fractions isolated from the cells of the murine typhoid salmonella by sedimentation with dihydrostreptomycine sulphate. Ribosomal fractions isolated from the murine typhoid salmonella transductants with the replaced rfb region failed to protect the animals from the infection with the virulent. S. typhimurium, S. enteritidis cultures. The virulence of the mutants and transductants was also changed in comparison with the initial strains.