RESUMEN
The effects of manganese (Mn) preconditioning, 96 h post-hatch followed by the replacement of inorganic Mn with different levels of organic Mn (5 to 21 D), on growth, tissue excreta Mn content, gene expression, and enzyme activity were evaluated. A total of 420 day-old male Cobb 500 broilers were divided into 2 groups. One group was fed a corn-soybean meal basal diet containing 17 mg of Mn/kg (preconditioning diet, MnPD); the second group was fed the non-preconditioning diet (NPCD), which was the MnPD supplemented with 60 mg of Mn/kg from manganese sulfate (MnSO4). On day 5, each group was divided into 5 subgroups and were randomly assigned to dietary treatments consisting of MnPD alone or MnPD supplemented with 12 or 60 mg Mn/kg Mn as MnSO4 or Mn proteinate (6 replicate cages of 6 birds). Broiler chicks that were fed the MnPD had lower (P ≤ 0.05) body weight gain (BWG) and G:F ratio when compared to those that were fed the NPCD for 4 D. Birds that were fed MnPD (1 to 4 D) and switched to MnPD supplemented with 60 mg/kg Mn (5 to 21 D) had lower (P ≤ 0.05) BWG compared to those that were fed NPCD (1 to 4 D) and switched to MnPD supplemented with 60 mg/kg Mn for 21 D. Excreta, tibia ash, liver, and heart Mn levels were increased (P ≤ 0.05) by supplemental Mn. The expression of jejunum divalent metal transporter-1 mRNA levels, as well as activities of plasma total super oxide dismutase and liver alanine transaminase, was not affected by MnPD or Mn source and levels. These results confirmed that feeding marginally deficient Mn diets to broiler chicks post-hatch does affect growth rate and tissue Mn concentration.
Asunto(s)
Pollos/fisiología , Compuestos de Manganeso/metabolismo , Sulfatos/metabolismo , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Pollos/crecimiento & desarrollo , Dieta/veterinaria , Suplementos Dietéticos/análisis , Relación Dosis-Respuesta a Droga , Expresión Génica , Masculino , Compuestos de Manganeso/administración & dosificación , Distribución Aleatoria , Sulfatos/administración & dosificación , Distribución TisularRESUMEN
For accurate estimation of nutrient digestibility, an ideal drying and sampling method is required to preserve the quality of the digesta. A standard corn-soybean meal (corn-SBM) broiler starter diet was fed from d 0 to 10 before birds were placed on the experimental diets until d 21. One hundred and sixty-eight male Cobb 500 broiler chicks were used to evaluate the effect of two drying methods (freeze-dryer vs. forced air-oven) and two drying temperatures (40 vs. 55°C) (Exp 1), while ninety-six chicks were used to evaluate the effect of flushing and squeezing as well as marker types (titanium vs. chromium) on apparent ileal DM, N, Ca, P, and AA digestibility (Exp 2). There were seven (Exp 1) or eight (Exp 2) replicate cages per treatment with 6 birds/cage. Digesta from the distal two thirds of the ileum was obtained from birds following euthanasia on d 21 by squeezing (Exp 1) and squeezing or flushing (Exp 2). Samples collected were stored in the freezer at -20°C until they were either freeze-dried (FD) or oven-dried (OD) at 40 or 55°C. There were no interactions between the drying methods and drying temperatures (Exp 1) on apparent ileal DM, N, and AA digestibility. Met had the highest (92.3%) while Cys had the lowest (73.8%) digestibility value. In Exp 2, no interaction between sampling methods and marker types was observed. The effect of sampling methods was not significant except for Arg and Met where squeezing resulted in higher (P < 0.05) digestibility values. Furthermore, apparent ileal His, Ile, Cys, Ser, and Tyr digestibility tended to be higher (P < 0.1) in squeezed digesta compared to the flushed digesta. Results from these studies showed that OD ileal digesta at 40 or 55°C had no negative effect on apparent ileal AA digestibility. Likewise, marker type did not influence apparent ileal AA digestibility values.
Asunto(s)
Alimentación Animal/análisis , Pollos/fisiología , Digestión , Manipulación de Alimentos/métodos , Aminoácidos/fisiología , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Dieta/veterinaria , Hordeum/química , Íleon/fisiología , Masculino , Distribución Aleatoria , Glycine max/química , Zea mays/químicaRESUMEN
Two studies were conducted to evaluate the effect of dietary Ca levels (low, 1% and high, 3%) on ileal endogenous amino acid losses (IEAAL) and standardized ileal amino acid digestibility (SIAAD) in broilers (BR) and laying hens (LH) fed nitrogen-free diets (NFD) and distiller's dried grain with solubles (DDGS)-based diets. A total of 384 male Cobb 500 BR and 288 LH were used in a completely randomized design (CRD) with 16 (BR) or 12 (LH) replicate cages with 6 birds/replicate. IEAAL and apparent ileal digestibility (AID) of AA were analyzed using the GLM procedure of SAS appropriate for a CRD while SIAAD values were analyzed using the GLM procedure of SAS appropriate for a 2 × 2 factorial arrangement of treatments. For BR, IEAAL and N losses (mg/kg of dry matter intake, DMI) were higher (P < 0.05) when NFD with high Ca level was fed (total AA was 39%, N was 35% higher). For most of the AA, AID was higher (P < 0.05) in BR fed DDGS-based diet with high Ca level. High dietary Ca resulted in higher (P < 0.05) SIAAD for all the AA except for Arg, Lys, Met, Cys, and Tyr. For LH, AID of AA was higher (P < 0.05) for the DDGS diet with high Ca level in 13 of the 18 AA evaluated. There was interaction (P < 0.05) between diet Ca level and correction method on LH SIAAD values for Thr, Asp, Gly, and Ser. The SIAAD values for 8 AA were higher (P < 0.05) in birds on high Ca DDGS diet. Correction with low Ca NFD resulted in higher (P < 0.05) SIAAD values for all the AA. Result from this study showed that high Ca increased total IEAAL in BR by 39% but decreased same by 27% in LH. Finally, SIAAD values were increased in BR fed high Ca DDGS-based diet while SIAAD value in LH was lower when correction was done using values from high Ca-NFD fed birds.
Asunto(s)
Aminoácidos/metabolismo , Calcio de la Dieta/metabolismo , Digestión , Íleon/fisiología , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales/efectos de los fármacos , Animales , Calcio de la Dieta/administración & dosificación , Pollos , Dieta/veterinaria , Digestión/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Femenino , MasculinoRESUMEN
The effect of dietary electrolyte balance (DEB), energy source (ES), and length of feeding of nitrogen-free diet (NFD) on ileal endogenous amino acid (EAA) loss in mg/kg dry matter intake (DMI) was evaluated in broiler chickens. In Experiment 1, 720 chickens consisting of 15 replicate cages with 6 chickens/replicate were used. Treatments were arranged in a 2 × 2 × 2 factorial and consisted of 4 NFD with 2 levels (low or high) of DEB and 2 ES [corn starch (CS) or dextrose (DX)], and 2 sampling time-points (diets were fed for either 72 h (d 16 to 19) or 120 h (d 16 to 21). Experiment 2 used 360 chickens in a 2 × 2 factorial arrangement of treatments with 2 levels (low or high) of DEB and 2 ES (CS or DX). Diets were fed for 72 h (d 18 to 21). All birds had access to feed and water on an ad libitum basis. Data were analyzed using the GLM procedure of SAS appropriate for a completely randomized design for a factorial arrangement of treatments. For Experiment 1, there were interactions (P < 0.05) between the 3 main factors for nitrogen and all the AA except Trp. Broilers that were fed DX-based NFD with high DEB for 72 h had the highest (P < 0.05) EAA losses. In Experiment 2, there was no interaction between DEB and ES except for His and Lys. When ileal EAA losses from birds fed the low DEB, CS-based NFD were used to standardize apparent ileal digestibility values from a previous study, there was no effect of length of feeding on standardized ileal AA digestibility values. In conclusion, DX-based NFD with high DEB increased endogenous AA loses. Despite differences in ileal EAA losses from CS-based NFD, standardized ileal AA digestibility values were not influenced by the length of feeding of NFD. Based on the results from these studies, NFD could be fed for 72 h without influencing SIAAD values.
Asunto(s)
Aminoácidos/fisiología , Pollos/fisiología , Íleon/fisiología , Nitrógeno/metabolismo , Equilibrio Hidroelectrolítico , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales/efectos de los fármacos , Animales , Dieta/veterinaria , Íleon/efectos de los fármacos , Distribución Aleatoria , Factores de Tiempo , Equilibrio Hidroelectrolítico/efectos de los fármacosRESUMEN
The goal of this study was to determine the effects of feeding a zinc (Zn) deficient diet to broiler chicks for 96 h post-hatch followed by feeding diets with different Zn sources and supplemental levels (5 to 21 d) on the growth performance, tissue, and excreta Zn content. At the start of the study, four hundred 20-day-old male broiler chicks were divided into two groups. One group was fed a corn soybean meal based diet containing 25 mg of Zn/kg (imprinting diet, ID). The second group was fed the basal diet supplemented with 40 mg of Zn/kg from Zn oxide (ZnO) (non-imprinting diet, NID). Both groups were fed these diets for 96 h. At d 5, chicks from each group were randomly assigned to the dietary treatments consisting of the basal diet alone or the basal diet supplemented with 8 or 40 mg/kg Zn as ZnO or Zn proteinate. Main effects of post-hatch Zn ID were observed on feed intake and G:F. ID decreased (P < 0.05) feed intake and improved (P < 0.05) the gain to feed ratio (G:F) of 14 and 21 d old chicks compared to G:F of chicks fed NID. Additionally, G:F for 14 and 21 d was improved (P < 0.05) by interaction of Zn source × level. Furthermore, at d 21 chicks fed the ID had a lower (P < 0.05) Zn content in the tibia ash and excreta, and a higher (P < 0.05) Zn content in the pancreas tissue compared to chicks fed NID. These results suggest that Zn imprinting can affect body Zn stores and early performance.
Asunto(s)
Fenómenos Fisiológicos Nutricionales de los Animales , Pollos/crecimiento & desarrollo , Pollos/metabolismo , Suplementos Dietéticos , Óxido de Zinc/metabolismo , Zinc/metabolismo , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Suplementos Dietéticos/análisis , Heces/química , Compuestos Inorgánicos/administración & dosificación , Compuestos Inorgánicos/metabolismo , Masculino , Compuestos Orgánicos/administración & dosificación , Compuestos Orgánicos/metabolismo , Distribución Aleatoria , Zinc/administración & dosificación , Zinc/deficiencia , Óxido de Zinc/administración & dosificaciónRESUMEN
Mendelian primary immunodeficiency diseases (MPIDs) are rare disorders affecting distinct constituents of the innate and adaptive immune system. Although they are genetically heterogeneous, a substantial group of MPIDs is due to mutations in genes affecting the nuclear factor-κB (NF-κB) transcription pathway, essential for cell proliferation and cell survival and involved in innate immunity and inflammation. Many of these genes encode for crucial regulatory components of the NF-κB pathway and their mutations are associated with immunological and developmental signs somehow overlapping in patients with MPIDs. At present, nine different MPIDs listed in the online mendelian inheritance in man (OMIM) are caused by mutations in at least nine different genes strictly involved in the NF-κB pathway that result in defects in immune responses. Here we report on the distinct function of each causative gene, on the impaired NF-κB step and more in general on the molecular mechanisms underlining the pathogenesis of the disease. Overall, the MPIDs affecting the NF-κB signalosome require a careful integrated diagnosis and appropriate genetic tests to be molecularly identified. Their discovery at an ever-increasing rate will help establish a common therapeutic strategy for a subclass of immunodeficient patients.
Asunto(s)
Síndromes de Inmunodeficiencia/genética , Síndromes de Inmunodeficiencia/metabolismo , FN-kappa B/metabolismo , Displasia Ectodérmica/genética , Displasia Ectodérmica/inmunología , Genes Recesivos , Humanos , Quinasa I-kappa B/genética , Quinasa I-kappa B/metabolismo , Síndromes de Inmunodeficiencia/inmunología , Quinasas Asociadas a Receptores de Interleucina-1/genética , Quinasas Asociadas a Receptores de Interleucina-1/inmunología , Mutación , FN-kappa B/genética , Enfermedades de Inmunodeficiencia Primaria , Enfermedades Raras/genética , Enfermedades Raras/inmunología , Transducción de Señal/genéticaRESUMEN
Alterations in nutrient intake in the avian neonatal posthatch period can impact development, performance, and metabolism in adulthood. Very little is known about how mineral levels during the post-hatch period affect or "program" gene expression patterns later in life. The objective of this study was to determine the effect of post-hatch (0 to 96 h) dietary mineral supplementation on performance, tissue mineral content, and intestinal gene expression profiles in 21-day-old broiler chicks. One-day-old chicks were randomly assigned to one of two treatment groups consisting of N (organic Zn, Cu, and Mn provided at 100 % of recommendations (National Research Council 1994)) and/or L (organic Zn, Cu, and Mn provided at 20 % of recommendations (National Research Council 1994)) diets fed in two intervals (days 14, days 521) as follows: (1)NLor (2)LL. Performance parameters did not differ between treatments except that body weight gain was greater (P < 0.05) in LL birds than NL birds over the experimental period. Bone mineral content was similar for both treatments at day 21. Intestinal gene expression profiling was examined using the Affymetrix GeneChip Chicken genome array. Ingenuity pathway analysis revealed differences in gene expression profiles between N and L treatments at day 5. At day 21, profiles were unique between NL and LL, suggesting that the diet fed until day 4 had an impact on gene expression patterns at day 21 even when birds were fed the same diets day 5day 21. In this study, we demonstrated that diets fed for the 96 h post-hatch had long-term effects on gene expression, providing unique information as to why post-hatch diets are so important for the longterm bird health and productivity.
Asunto(s)
Pollos/metabolismo , Regulación de la Expresión Génica/fisiología , Mucosa Intestinal/metabolismo , Oligoelementos/metabolismo , Aumento de Peso/fisiología , Animales , Pollos/crecimiento & desarrollo , Femenino , Perfilación de la Expresión Génica , Regulación de la Expresión Génica/efectos de los fármacos , Intestinos/crecimiento & desarrollo , Masculino , Análisis de Secuencia por Matrices de Oligonucleótidos , Oligoelementos/farmacología , Aumento de Peso/efectos de los fármacosRESUMEN
The use of mannan-oligosaccharides (MOS) as alternatives to antibiotic growth promoters (AGP) has gained in popularity in recent years due to regulatory restrictions of using AGP in food animal production. Benefits of MOS usage include improvement on animal performance, feed efficiency, and gastrointestinal health. The molecular mechanisms of these functions however are not clear. The goal of the current study was to use a transcriptomics approach to investigate the effects of MOS on the intestinal gene expression profile of young broilers and characterize biological gene pathways responsible for the actions of MOS. One hundred and twenty 1-d-old Cobb 500 broiler chicks were randomly divided into 2 groups and were fed either a standard wheat-soybean meal-based (control) diet or the same diet supplemented with 2.2 g/kg of MOS (Bio-Mos, Alltech, Nicholasville, KY) for 3 wk, followed by jejunal gene expression profiling analysis using chicken-specific Affymetrix microarrays. Results indicated that a total of 672 genes were differentially expressed (P < 0.01 and fold change >1.2) in the jejunum by MOS supplementation. Association analysis indicated that differentially expressed genes are involved in diverse biological functions including energy production, cell death, and protein translation. Expression of 77 protein synthesis-related genes was differentially regulated by MOS in the jejunum. Further pathway analysis indicated that 15 genes related to oxidative phosphorylation were upregulated in the jejunum, and expression of genes important in cellular stress response, such as peroxiredoxin 1, superoxide dismutase 1, and thioredoxin, were also increased by MOS. Differential expression of genes associated with cellular immune processes, including lysozyme, lumican, ß 2-microglobin, apolipoprotein A-1, and fibronectin 1, were also observed in MOS-fed broilers. In summary, this study systematically identified biological functions and gene pathways that are important in mediating the biological effects of MOS in broilers.
Asunto(s)
Pared Celular/química , Pollos/metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Yeyuno/metabolismo , Mananos/farmacología , Levaduras/química , Envejecimiento , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Dieta/veterinaria , Suplementos Dietéticos , Perfilación de la Expresión Génica , Yeyuno/efectos de los fármacos , Mananos/química , Análisis por Matrices de Proteínas/veterinaria , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinariaRESUMEN
1. Two studies were conducted to investigate the effect of feeding different concentration and forms of zinc (Zn) on the performance and tibia Zn status of broiler chicks. 2. In Experiment 1, chicks fed on the control or the diet supplemented with 12?mg of Zn as sulphate had lower feed intake, weight gain and tibia Zn content than other treatment groups. Chicks given 12 and 24 mg of organic Zn in starter and grower phases, respectively, had the same performance and tibia Zn content as those fed 40 mg of Zn as sulphate and the same performance but higher tibia Zn content than those given 12 mg of Zn as organic over the 42 d. 3. In Experiment 2, chicks given 24 mg organic Zn had greater weight gain than chicks fed on the other treatment diets in the starter period. Chicks fed on the control diet had lower tibia Zn content than chicks fed other treatment diets. Chicks given 80 mg Zn as sulphate had higher tibia Zn content than chicks fed the other treatment diets except those given 40 mg of Zn as sulphate. 4. The results from these trials indicate that feeding lower concentration of Zn as organic form may better promote the growth performance of broiler chicks.
Asunto(s)
Alimentación Animal/análisis , Pollos/crecimiento & desarrollo , Pollos/metabolismo , Dieta/veterinaria , Tibia/química , Zinc/administración & dosificación , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Suplementos Dietéticos , Relación Dosis-Respuesta a Droga , Kentucky , Masculino , Distribución Aleatoria , Aumento de Peso , Zinc/análisis , Zinc/metabolismoAsunto(s)
Resfriado Común/fisiopatología , Resfriado Común/virología , Hipersensibilidad/fisiopatología , Ruidos Respiratorios/clasificación , Alérgenos/inmunología , Niño , Preescolar , Estudios de Cohortes , Ejercicio Físico , Femenino , Humanos , Lactante , Masculino , Fenotipo , Prevalencia , Reino Unido/epidemiologíaRESUMEN
Selenium (Se) is an essential component of at least 25 selenoproteins involved in a multitude of physiological functions, including reproduction. However, relatively little is known about the mechanisms by which Se exerts its physiological effects in reproductive tissue. The objective of this study was to compare the effect of long-term inorganic Se (sodium selenite, SS) and organic yeast-derived Se (Sel-Plex(®), SP) supplementations on tissue Se content and gene expression patterns in the oviduct of broiler-breeder hens. Hens were randomly assigned at 6 weeks of age to one of the three treatments: basal semi-purified diet (control), basal diet+0.3 ppm Se as SP or basal diet+0.3 ppm Se as SS. At 49 weeks, oviduct tissue from hens randomly selected from each treatment (n=7) was analyzed for Se content and gene expression profiles using the Affymetrix Chicken genome array. Gene expression data were evaluated using GeneSpring GX 10.0 (Silicon Genetics, Redwood, CA) and Ingenuity Pathways Analysis software (Ingenuity Systems, Redwood City, CA). Oviduct Se concentration was greater with Se supplementation compared with the control (P≤0.05) but did not differ between SS- and SP-supplemented groups. Gene expression analysis revealed that the quantity of gene transcripts associated with energy production and protein translation were greater in the oviduct with SP but not SS supplementation. Targets up-regulated by SP, but not SS, included genes encoding several subunits of the mitochondrial respiratory complexes, ubiquinone production and ribosomal subunits. SS hens showed a decrease in transcripts of genes involved in respiratory complexes, ATP synthesis and protein translation and metabolism in oviduct relative to control hens. In this study, although tissue Se concentrations did not differ between hens fed SS- and SP-supplemented diets, expression patterns of genes involved in energy production and protein synthesis pathways differed between treatments. These variations may partially explain the differences in reproductive performance reported in hens fed different forms of Se.
Asunto(s)
Pollos/genética , Regulación de la Expresión Génica/efectos de los fármacos , Oviductos/efectos de los fármacos , Selenio/administración & dosificación , Animales , Pollos/metabolismo , Biología Computacional , Suplementos Dietéticos , Femenino , Perfilación de la Expresión Génica/métodos , Perfilación de la Expresión Génica/veterinaria , Análisis de Secuencia por Matrices de Oligonucleótidos/veterinaria , Oviductos/metabolismo , Oviductos/fisiología , ARN Mensajero/biosíntesis , ARN Mensajero/genética , Distribución Aleatoria , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinariaRESUMEN
BACKGROUND: The oocyte-to-embryo transition (OET) requires a co-ordinated transcriptional programme acting through evolutionarily conserved events, and transcription factors (TFs) are known to control these processes. Here, we focus on nuclear factor (NF)-κB, a TF involved in several cellular processes, studying NFκB-inhibitor (NFKBIA) mRNA and its protein product, IκBα, during OET. NFKBIA and IκBα are part of a regulatory loop, as IκBα is the major down-regulator of NF-κB activation while NFKBIA transcription is activated by NF-κB. METHODS AND RESULTS: We found a dynamic correlation between NFKBIA transcript, expression of IκBα-protein and activation of NF-κB/p65 in bovine oocyte and embryo. During the transition from immature to in vitro matured bovine oocyte, we observed a decrease in maternal NFKBIA mRNA and a parallel increase of the IκBα-protein (both P < 0.05). In the embryo, NFKBIA neo-synthesis is activated as a consequence of embryo genome activation (EGA), and IκBα decreases. NF-κB/p65-binding activity was detectable at low levels in immature oocyte, disappeared in dormant metaphase II oocyte and was strong in the embryo, during embryonic NFKBIA synthesis. The level of NF-κB/p65 DNA binding correlates with the timing of meiotic silencing during bovine oocyte maturation and embryonic transcription reprogramming. CONCLUSIONS: The IκBα/NF-κB circuit appears to be a tightly stage-controlled mechanism that could govern OET, being activated at EGA. Our findings represent the first characterization of NFKBIA and IκBα as maternal effectors in both the bovine oocyte and embryo. We suggest a role for NFKBIA as a marker of NF-κB/p65 activation in the human oocyte and early embryo.
Asunto(s)
Desarrollo Embrionario/fisiología , Proteínas I-kappa B/fisiología , FN-kappa B/metabolismo , Oocitos/crecimiento & desarrollo , Factor de Transcripción ReIA/metabolismo , Secuencia de Aminoácidos , Animales , Bovinos , Desarrollo Embrionario/genética , Activación Enzimática , Proteínas I-kappa B/análisis , Proteínas I-kappa B/metabolismo , Datos de Secuencia Molecular , Inhibidor NF-kappaB alfa , Oocitos/metabolismo , Estructura Terciaria de Proteína , ARN Mensajero/metabolismo , Alineación de Secuencia , Factor de Transcripción ReIA/análisis , Factor de Transcripción ReIA/fisiologíaRESUMEN
A study was conducted to investigate the effects of feeding inorganic or organic Zn and Cu on the performance and tissue mineral content of chicks. A corn-soybean meal diet without Cu and Zn supplementation, containing 31 mg of Zn/kg of diet and 6.6 mg of Cu/kg of diet, was used as a basal diet. Organic Zn (a chelated Zn proteinate) and organic Cu (a chelated Cu proteinate) were used as organic sources for comparison with inorganic reagent-grade sulfates. Supplements provided 20 mg/kg of Zn and 8 mg/kg of Cu. A 3x3 factorial arrangement of treatments consisting of feeding the basal diet with 3 supplements (none, sulfate, or organic) of Cu and of Zn was used. Ten groups of 6 one-day-old male broilers were assigned to each of 9 dietary treatments. Tap water and feed were supplied on an ad libitum basis during the 3-wk trial. The Zn and Cu content in the mucosa of the duodenum of the chick was determined. Dietary supplementation of Zn increased (P<0.01) weight gain, feed intake, and G:F of chicks. The G:F for chicks fed both inorganic sources of Zn and Cu was lower (P<0.01) than that for chicks fed only the inorganic source of Zn. Dietary inclusion of Zn increased (P<0.01) tibia and plasma Zn content. The tibia Zn content for chicks fed organic Zn was higher (P<0.01) than that for chicks fed inorganic Zn. Liver Cu content was decreased (P<0.05) by dietary inclusion of Zn. The Zn and Cu contents in the mucosa of chicks fed the organic source were higher (P<0.01) than those of chicks fed the control diet. The feed conversion data suggest that the antagonism between Zn and Cu occurred when the inorganic forms, but not organic forms, of these 2 minerals were included in a chick diet.
Asunto(s)
Fenómenos Fisiológicos Nutricionales de los Animales/fisiología , Quelantes/farmacología , Pollos/metabolismo , Cobre/metabolismo , Zinc/metabolismo , Animales , Peso Corporal/fisiología , Cobre/sangre , Duodeno/química , Ingestión de Alimentos/fisiología , Absorción Intestinal/fisiología , Hígado/química , Masculino , Distribución Aleatoria , Tibia/química , Zinc/sangreAsunto(s)
Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/microbiología , Ehrlichia/aislamiento & purificación , Ehrlichiosis/veterinaria , Animales , Sangre/microbiología , Brasil/epidemiología , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Perros , Ehrlichia/genética , Ehrlichiosis/epidemiología , Ehrlichiosis/microbiología , Incidencia , Reacción en Cadena de la PolimerasaRESUMEN
Two experiments were conducted to examine the effects of alpha-galactosidase supplementation and acidification of diets on nutrient digestibility and growth performance of broiler chicks. In experiment 1, dietary treatments consisted of feeding a low-energy basal diet (2.74 Mcal of ME/kg) alone, the basal diet supplemented with 1,724 units of alpha-galactosidase per kg, the basal diet supplemented with 2% citric acid, or the basal diet supplemented with both. alpha-Galactosidase significantly increased feed intake, weight gain, AME(n) of the diets, and retention of CP and neutral detergent fiber (NDF) (P < 0.05). Citric acid significantly increased the retention of DM, CP, and NDF, but decreased feed intake and weight gain. The greatest values for DM and NDF retention and for AME(n) were obtained with the combination of alpha-galactosidase plus citric acid. In experiment 2, chicks were fed diets with 2 levels of energy (2.74 or 3.11 Mcal/kg), 2 levels of citric acid (0 or 1.5%), and 2 levels of alpha-galactosidase (0 or 1,724 units/kg) in a 2 x 2 x 2 factorial arrangement of treatments. alpha-Galactosidase significantly increased the reducing sugar concentration in the crop content, whereas citric acid decreased the pH and increased the reducing sugar concentration in the crop content. Citric acid decreased the gain to feed ratio in the absence but not in the presence of alpha-galactosidase. The data from these studies indicate that acidification of diet improves the efficacy of alpha-galactosidase.
Asunto(s)
Alimentación Animal/análisis , Pollos/crecimiento & desarrollo , Ácido Cítrico/farmacología , Dieta/veterinaria , Digestión/efectos de los fármacos , alfa-Galactosidasa/farmacología , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Digestión/fisiología , Metabolismo Energético , Concentración de Iones de Hidrógeno , MasculinoRESUMEN
1. The purpose of this study was to investigate the effects of Bioplex Zn (a chelated zinc proteinate) and phytase supplementation in a maize-soybean meal diet on the performance and tissue zinc (Zn) content of broiler chicks. Treatment structure consisted of a 2 x 6 factorial arrangement with two inclusions of phytase (0 or 500 PU/kg) and 6 of Bioplex Zn providing 0, 2, 4, 8, 16 and 32 mg Zn/kg diet. A total of 864 chicks were randomly assigned to each of 12 dietary treatments with 6 replicate cages of 12 chicks. 2. Dietary inclusion of phytase increased feed intake, weight gain, plasma Zn content, tibia Zn content, tibia and ash weight. 3. Dietary supplementation of Bioplex Zn linearly increased feed intake, weight gain, gain to feed ratio, plasma Zn concentration, liver Zn concentration, tibia Zn content, tibia and ash weight. 4. An interactive effect of phytase and Bioplex Zn on feed intake, weight gain, tibia Zn concentration and tibia ash weight was found. 5. One slope, straight broken-line analysis of weight gain regressed on the supplemental Zn level provided as Bioplex Zn indicated that 12 mg/kg supplemental Zn without phytase and 7.4 mg/kg supplemental Zn with phytase were required for the optimal weight gain of chicks.
Asunto(s)
6-Fitasa/farmacología , Alimentación Animal/análisis , Pollos/crecimiento & desarrollo , Glycine max , Zea mays , Zinc/análisis , Zinc/farmacología , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Pollos/metabolismo , Dieta/veterinaria , Suplementos Dietéticos , Relación Dosis-Respuesta a Droga , Masculino , Aumento de Peso , Zinc/metabolismoRESUMEN
We studied the effect of dietary source (organic or inorganic) and level of Se on the Se uptake of chick embryos. After receiving a low-Se diet for 16 wk, 126 Leghorn laying hens were randomly assigned to one of seven dietary treatments. Treatments consisted of feeding a low-Se basal diet alone or with one of three levels of added Se (0.1,0.2, or 0.3 mg/kg Se) supplied by sodium selenite or Se-enriched yeast. Fertile eggs were collected after 33 d of feeding the experimental diets. Eggs were subjected to no incubation or incubation for 5, 10, 15, or 20 d. Non-incubated eggs were separated, and the yolk and albumen were assayed separately for Se. Incubated eggs were separated into the embryo and extra-embryonic portions, which were assayed separately for Se. Se concentrations of the yolk and albumen were significantly different among dietary treatments. Compared with eggs from hens fed sodium selenite, yolk and albumen Se concentrations were higher in eggs from hens fed Se yeast. Embryonic and extra-embryonic Se concentrations were higher in eggs from hens fed Se yeast than eggs from hens fed sodium selenite. The largest increase in embryonic Se concentration was observed during Days 10 to 15 of incubation. It was concluded that Se source and dietary inclusion level influenced the Se concentration of portions of developing embryonated eggs and that embryonic Se concentration changed during incubation.
Asunto(s)
Embrión de Pollo/metabolismo , Pollos/metabolismo , Dieta , Selenio/administración & dosificación , Selenio/farmacocinética , Animales , Embrión de Pollo/química , Clara de Huevo/análisis , Yema de Huevo/química , Femenino , Selenio/análisis , Selenito de Sodio/administración & dosificación , Factores de Tiempo , Levadura SecaRESUMEN
An experiment was conducted to determine the effect of diets with reduced CP and supplemental amino acids on broiler performance, N excretion, litter characteristics, and equilibrium NH3 gas concentration. Results suggest that reducing CP (and lysine) below 241 g/kg (13.7 g/kg lysine) in the diets fed during the first 3 wk may slightly increase feed:gain and therefore may not be advisable. During the period 22 to 43 d of age there were no significant differences in weight gain and BW at 6 wk of age when reducing CP from 215 g/kg (11.5 g/kg lysine) to 196 g/kg (11.3 g/kg lysine), but feed intake and feed:gain ratio increased. However, reducing CP did cause equilibrium NH3 gas concentration and litter N to decline by 31 and 16.5%, respectively. Both of these advantages will improve air quality within the housing facility and possibly reduce heating costs during winter associated with higher ventilation rates required to reduce elevated NH3 gas concentrations.
Asunto(s)
Amoníaco/análisis , Alimentación Animal , Pollos/crecimiento & desarrollo , Proteínas en la Dieta , Aumento de Peso , Envejecimiento , Aminoácidos , Animales , Alimentos Fortificados , Lisina , Masculino , Residuos/análisisRESUMEN
An experiment was conducted to determine whether broiler litter concentration of N and P and equilibrium NH3 gas concentration can be reduced by reducing dietary CP and P levels and supplementing with amino acids and phytase, respectively, without adversely affecting bird performance. Equilibrium NH3 gas concentration above the litter was measured. The experiment was divided into a starter period (1 to 21 d) and grower period (22 to 42 d), each having two different CP and P levels in a 2 x 2 factorial arrangement. The CP treatments consisted of a control with a mean CP of 204 and 202 g/kg for starter and grower periods, respectively, and a low CP diet with means of 188 and 183 g/kg, respectively, but with similar amino acid levels as the control. The P treatments comprised starter and grower control diets containing means of 6.7 and 6.3 g/kg P, respectively, and low P treatment means of 5.8 and 5.4 g/kg P supplemented with 1.0 g/kg phytase. Reducing starter diet CP by 16 g/kg reduced weight gain by 3.5% and, hence, body weight at 21 d of age, but did not affect feed intake or feed efficiency. Reducing P did not affect feed intake and weight gain, but improved feed efficiency by 2.0%. Responses in feed intake and efficiency to CP depended on the level of dietary P. For the grower period there were no significant differences in feed intake, weight gain, and feed efficiency, nor in body weight at 42 d of age, after correcting for 21-d body weight, between CP and P treatments. There were significant (P < 0.001) reductions in litter N and P concentrations, but not equilibrium NH3 gas concentration, moisture content, or pH, for low CP and P diets. Mean equilibrium NH3 gas concentration was 63 ppm. Litter N concentration was reduced 16.3% with the low CP diets, and litter P by 23.2% in low P treatments. The results suggest that dietary manipulation shows merit for reducing litter N and P concentrations while maintaining acceptable production performance from broilers.