RESUMEN
All members of the ant genus Odontomachus Latreille, 1804 are venomous ants. Four species in this genus have been identified from Thailand: Odontomachus latidens Mayr, 1867; O. monticola Emery, 1892; O. rixosus Smith, 1757; and O. simillimus Smith, 1758. The three latter species are available and have been used for an outline morphometric study. They display similar morphology, which makes their distinction very difficult except for highly qualified individuals. A total of 80 worker specimens were studied, exploring the contour shapes of their head and pronotum as possible taxonomic characters. The size of each body part was estimated determining the contour perimeter, the values for which were largely overlapping between O. rixosus and O. simillimus; most O. monticola specimens exhibited a significantly larger size. In contrast to the size, each contour shape of the head or pronotum established O. rixosus as the most distinct species. An exploratory data analysis disclosed the higher taxonomic signal of the head contour relative to the pronotum one. The scores obtained for validated reclassification were much better for the head (99%) than for the pronotum (82%). This study supports outline morphometrics of the head as a promising approach to contribute to the morphological identification of ant species, at least for monomorphic workers.
RESUMEN
Stemona collinsiae exhibits insecticidal resistance against various pests and insect vectors. However, insecticidal activity of S. collinsiae roots has not been tested for some insect vectors, including the American cockroach, Periplaneta americana. The synanthropic insect P. americana is a reservoir of pathogenic and non-pathogenic microorganisms and a cause of infectious diseases and cockroach allergy. This important vector transmits microorganisms to animals and humans to cause vector-borne diseases. This research involved detection of the nymphicidal and adulticidal activities of S. collinsiae root extracts against P. americana through oral administration. The effects of hexane, dichloromethane, ethanol, and water crude extracts were tested on final instar nymphs and adult P. americana. After P. americana ingested bait containing hexane and dichloromethane crude extracts, signs of toxicity occurred, such as hind leg shaking, whole-body tremor, immobility, abdomen swelling, and death. At 48 h, the nymphs and adult P. americana that ingested dichloromethane crude extract-containing bait showed corrected mortality of 65%-100% and 20%-100%, respectively. Whereas none of the nymphs and adult P. americana that ingested the water crude extract-containing bait died (0% corrected mortality). When we dissected alimentary canals of the dead P. americana that had ingested dichloromethane and hexane crude extract-containing baits, the foreguts were found to be swollen. TLC analysis showed the dichloromethane and hexane crude extracts contained the alkaloid didehydrostemofoline and unknown fluorescent substances. Phytochemicals from crude extracts were detected in extracts of dissected alimentary canals using thin-layer chromatography, and didehydrostemofoline alkaloid and unknown fluorescent substances were found in cockroaches that ingested dichloromethane- and hexane-containing baits. The cause of death of P. americana may be attributed to alkaloids and synergistic effects of other substances in S. collinsiae root extract. Mechanisms of action might include several pathways involved in nervous system function. Thus, dichloromethane and hexane crude extracts can be developed as alternative active ingredients in a natural insecticide for cockroach control.
