RESUMEN
The ciliated protozoan Cryptocaryon irritans infects a wide range of marine fish and causes the highly lethal white spot disease. This parasite possesses three morphologically and physiologically distinct life stages: an infectious theront, a parasitic trophont, and an asexually reproductive tomont. In the past few years, several attempts have been made to help elucidate how C. irritans transforms from one stage to another using transcriptomic or proteomic approaches. However, there has been no research studying changes in transcription profiles between different time points of a single C. irritans life stage-the development of this parasite. Here we use RNA-seq and compare gene expression profiles of theront cells collected by 1 and 10 hrs after they emerged from tomonts. It has been shown that infectivity of theront cells declines 6-8 hours post-emergence, and we used this characteristic as a physiological marker to confirm the aging of theront cells. We identified a total of 41 upregulated and 90 downregulated genes that were differentially expressed between young and aging theront cells. Using Blast2Go to further analyze functions of these genes, we show that genes related to energy production are downregulated, but quite surprisingly many genes involved in transcription/translation processes are upregulated. We also show that expression of all nine detectable agglutination/immobilization antigen genes, with great sequence divergence, is invariably downregulated. Functions of other differentially expressed genes and indications are also discussed in our study.
Asunto(s)
Envejecimiento/metabolismo , Cilióforos/metabolismo , Cilióforos/patogenicidad , Animales , Cilióforos/genética , Cilióforos/crecimiento & desarrollo , Infecciones por Cilióforos/veterinaria , Enfermedades de los Peces/parasitología , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Perciformes , Análisis de Secuencia de ARN , TranscriptomaRESUMEN
Se realizó un estudio descriptivo con el objetivo de determinar la prevalencia de infección en 195 pacientes ingresados en la Unidad de Quemados Pearl F. Ort del Hospital Dr. Luis E. Aybar durante el período febrero de 1993 a febrero de 1994. Encontrándose que el 54.4// de los casos desarrolló infección; el microorganismo aislado con mayor frecuencia fue la Pseudomona aeruginosa, con 34.6//; en el 28.3// la infección se localizó en la quemadura y el 30.2// del total de los pacientes fallecieron