RESUMEN
We studied the nuclear localization and relative position in the nuclear space of malignant translocation partner genes c-Myc, CCND1, and IGH locus in naive and differentiating B cells. We have shown that, during B-cell maturation, c-Myc and IGH loci become closer to each other. In differentiating lymphocytes, those alleles of c-Myc and IGH that are in close spatial proximity to each other are closer to the nucleolus. For the CCND1 locus, no correlation between the proximity of loci and nuclear localization was found. These data suggest that the close spatial proximity of c-Myc and IGH loci during B-cell maturation increase the probability of malignant translocation.
Asunto(s)
Linfocitos B/metabolismo , Diferenciación Celular/fisiología , Núcleo Celular/metabolismo , Ciclina D1/metabolismo , Cadenas Pesadas de Inmunoglobulina/metabolismo , Proteínas Proto-Oncogénicas c-myc/metabolismo , Transporte Activo de Núcleo Celular , Alelos , Linfocitos B/citología , Sitios Genéticos , Cadenas Pesadas de Inmunoglobulina/genéticaRESUMEN
The karyotype of the chicken Gallus gallus domesticus was studied by means of pulsed-field electrophoresis. An electrokaryogram was obtained for the microchromosomal (MI) portion of the chicken genome. Chicken MIs were separated into two fractions. A fraction with a higher mobility included MIs sized 3.4-4.8 Mb; the lower size limit of a less mobile fraction corresponded to MIs of approximately 5 Mb. The smallest MI in the chicken karyotype was estimated at 3.4 +/- 0.25 Mb.