In silico analysis as a strategy to identify candidate epitopes with human IgG reactivity to study Porphyromonas gingivalis virulence factors.

Porphyromonas gingivalis (Pg) is one of the main pathogens in chronic periodontitis (CP). Studies on the immunogenicity of its virulence factors may contribute to understanding the host response to infection. The present study aimed to use in silico analysis as a tool to identify epitopes from Lys-gingipain (Kgp) and neuraminidase virulence factors of the Pg ATCC 33277 strain. Protein sequences were obtained from the NCBI Protein Database and they were scanned for amino acid patterns indicative of MHC II binding using the MHC-II Binding Predictions tool from the Immune Epitope Database (IEDB). Peptides from different regions of the proteins were chemically synthesized and tested by the indirect ELISA method to verify IgG immunoreactivity in serum of subjects with CP and without periodontitis (WP). T cell epitope prediction resulted in 16 peptide sequences from Kgp and 18 peptide sequences from neuraminidase. All tested Kgp peptides exhibited IgG immunoreactivity whereas tested neuraminidase peptides presented low IgG immunoreactivity. Thus, the IgG reactivity to Kgp protein could be reaffirmed and the low IgG reactivity to Pg neuraminidase could be suggested. The novel peptide epitopes from Pg were useful to evaluate its immunoreactivity based on the IgG-mediated host response. In silico analysis was useful for preselecting epitopes for immune response studies in CP.

Abordagens cirúrgicas na região estética do tecido mole peri-implantar para corrigir um defeito crestal ao redor de implantes adjacentes / Surgical approaches at the peri-implant soft tissue esthetic region to correct a crestal defect around adjacent implants: case report

O sucesso de uma reabilitação com implantes depende não só de sua funcionalidade, mas também de uma estética que abranja desde a coroa sobre o implante ao tecido mole que o envolve, visando à manutenção das características teciduais de um dente natural. A cirurgia plástica peri-implantar passou a ser largamente utilizada para correções de defeitos ósseos e teciduais em áreas que sofreram remodelação tecidual extensa após a extração. Apesar da grande variedade de técnicas que podem ser utilizadas, existem limitações em relação à previsibilidade do resultado. No relato de caso apresentado, foram utilizadas três técnicas de cirurgia plástica para o ganho tecidual e a melhora estética do defeito de crista apresentado entre implantes anteriores.

The success of implant rehabilitation depends not only on its functionality, but also on an aesthetic that involves the implant crown and the soft tissue that surrounds it, aiming to maintain the tissue characteristics of a natural tooth. Perimplantar plastic surgery became widely used for corrections of bone and tissue defects in areas that underwent extensive tissue remodeling after extraction. Despite the wide range of techniques that can be used, there are limitations to the predictability of the outcome. In the case report presented, three techniques of plastic surgery were used for tissue gain and esthetic improvement of the crestal defect presented between adjacent implants in the anterior region.

Carga imediata em implantes unitários - Relato de dois casos clínicos / Immediate loading of single implants - Related two cases

O presente artigo tem como finalidade relatar dois casos clínicos de implantes unitários com carga imediata em dois pacientes, que receberam as próteses provisórias imediatamente depois da cirurgia. Os casos serão apresentados passo a passo e discutidos com a literatura pertinente.