Guides or guidelines for interacting and playing with medical complex children: a qualitative documentary research.

OBJECTIVES: to identify content on play and interaction with children with special health care needs recommended in clinical guidelines; analyze play and interaction activities applicable to children with special health care needs and complex care requirements. METHOD: qualitative documentary research based on guides, protocols, or guidelines on playing and interacting with children with special and living with complex care. Search terms in English (guidelines, playing OR play, complex needs, OR chronic disease) and in Portuguese ( guia, brincar ou brincadeiras, condições crônicas ) on the first ten pages of_Google Search ® . Thematic analysis was applied to the information extracted from the documents. RESULTS: a total of nine documents with similar content were grouped into units of analysis, keeping only the interacting and playing activities applicable to children with special health care needs and living with complex care requirements, namely stimulation of potential, stimulation of adult-child interaction, and stimulation of the senses (touch, sight, and hearing), to be carried out by health professionals and family caregivers in the different care contexts. CONCLUSION: interaction and play are potential promoters of adult-child interaction, with application in the stimulating and life-delivering complex care for children.

Guides or guidelines for interacting and playing with medical complex children: a qualitative documentary research / Guías o directrices para interactuar y jugar con niños con condiciones clínicas complejas: investigación cualitativa documental / Guias ou diretrizes para interagir e brincar com crianças clinicamente complexas: uma pesquisa qualitativa documental

Objectives: to identify content on play and interaction with children with special health care needs recommended in clinical guidelines; analyze play and interaction activities applicable to children with special health care needs and complex care requirements. Method: qualitative documentary research based on guides, protocols, or guidelines on playing and interacting with children with special and living with complex care. Search terms in English (guidelines, playing OR play, complex needs, OR chronic disease) and in Portuguese ( guia, brincar ou brincadeiras, condições crônicas ) on the first ten pages of_Google Search ® . Thematic analysis was applied to the information extracted from the documents. Results: a total of nine documents with similar content were grouped into units of analysis, keeping only the interacting and playing activities applicable to children with special health care needs and living with complex care requirements, namely stimulation of potential, stimulation of adult-child interaction, and stimulation of the senses (touch, sight, and hearing), to be carried out by health professionals and family caregivers in the different care contexts. Conclusion: interaction and play are potential promoters of adult-child interaction, with application in the stimulating and life-delivering complex care for children.

Objetivos: identificar contenido sobre juego e interacción con niños con necesidades de especiales atención en salud recomendados en guías clínicas; analizar las actividades de juego e interacción que se pueden implementar niños con necesidades especiales de atención en salud y demandas de cuidados clínicamente complejos. Método: investigación cualitativa documental basada en guías, protocolos o directrices para jugar e interactuar con niños con necesidades especiales de atención en salud. Búsqueda de los términos en inglés ( guidelines, playing o play, complex needs OR chronic disease ) y en portugués ( guia, brincar o brincadeiras, condições crônicas ), en las primeras 10 páginas de Google Search ® . Se aplicó análisis temático a la información extraída de los documentos. Resultados: se agruparon en unidades de análisis nueve documentos con contenido similar, se extrajeron solo las actividades para interactuar y jugar que se pueden implementar con niños con necesidades especiales de atención en salud y demandas de cuidados clínicamente complejos, a saber: estimular las potencialidades, estimular la interacción adulto-niño y estimular los sentidos (tacto, visión y oído), que realizan los profesionales de la salud y los cuidadores familiares en diferentes contextos de cuidado. Conclusión: interactuar y jugar pueden promover la interacción adulto-niño e implementarse en el cuidado estimulante y vivificante de niños con condiciones clínicas complejas.

Objetivos: identificar conteúdos sobre brincar e interagir com crianças com necessidades de saúde especiais recomendados em guias clínicos; analisar as atividades de brincar e interagir aplicáveis às crianças com necessidades de saúde especiais e demandas de cuidados clinicamente complexas. Método: pesquisa qualitativa documental baseada em guias, protocolos ou diretrizes sobre brincar e interagir com crianças com necessidades de saúde especiais. Busca dos termos em inglês ( guidelines, playing ou play, complex needs OR chronic disease ) e em português (guia, brincar ou brincadeiras, condições crônicas), nas 10 primeiras páginas do Google Search ® . Aplicou-se a análise temática às informações extraídas dos documentos. Resultados: agruparam-se nove documentos com conteúdo similares em unidades de análise, mantendo-se somente as atividades do interagir e brincar aplicáveis às crianças com necessidades de saúde especiais e demandas de cuidados clinicamente complexas, a saber: estimulação das potencialidades, estimulação da interação adulto-criança e estimulação dos sentidos (tato, visão e audição), a serem realizadas por profissionais de saúde e familiares cuidadores nos diferentes contextos de cuidado. Conclusão: o interagir e o brincar são potenciais promotores da interação adulto-criança, com aplicação no cuidado estimulador e vivificante de crianças clinicamente complexas.

¿La Entrevista Motivacional puede ser utilizada en la prevención de las caries en preescolares? Una revisión integradora / A Entrevista Motivacional pode ser utilizada na prevenção da cárie em pré-escolares? Uma revisão integrativa / Can Motivational Interviewing be used in caries prevention in preschoolers? An integrative review

El objetivo fue realizar una revisión a partir de una duda clínica: "¿La práctica de la entrevista motivacional (EM) puede ser utilizada en la prevención de las caries en preescolares?" Materiales y métodos: se realizó una revisión integradora de la literatura en abril de 2021 utilizándose una adaptación del "Sistema 6S". Se evaluó la calidad de las revisiones sistemáticas (RS). Fueron realizadas búsquedas en las revistas periódicas Evidence-Based Dentistry, Journal of Evidence-Based Dental Practice y en el sitio del Centro de Odontología Basada en Evidencias de American Dental Associaton, Biblioteca Cochrane y PubMed/Medline. Las palabras para la búsqueda fueron "motivational interviewing" y "dental caries". Resultados: Se hallaron 2 RS sobre el tema. Una RS de 2020 mostró reducción de caries a través del meta-análisis, pero sin diferencia estadísticamente significativa; los autores destacan que las evidencias limitadas se deben a la presencia de estudios heterogéneos basados en diferentes metodologías. Otra RS de 2021 concluyó que la EM posee el potencial de modificar el comportamiento y reducir significativamente lesiones cariosas principalmente en niños con elevada experiencia de caries. Fue también evaluada la calidad metodológica de las RS incluidas en esta revisión a través de los protocolos PRISMA y AMSTAR-2. Ambas presentaron bajo riesgo de sesgo y alta calidad. Conclusión: la práctica de la EM contribuyó en la prevención de la caries en preescolares, principalmente en los niños de alto riesgo. Sin embargo, las evidencias son moderadas, obtenidas a partir de meta-análisis poco robustos y de un número limitado de estudios aleatorios de buena calidad metodológica

Objetivou-se realizar uma revisão integrativa a partir de uma dúvida clínica: "A prática da entrevista motivacional (EM) pode ser utilizada na prevenção da cárie dentária em pré-escolares?". Materiais e métodos: foi realizada uma revisão integrativa da literatura em abril de 2021 utilizando-se uma adaptação do "Sistema 6S". Avaliou-se a qualidade das revisões sistemáticas (RS) encontradas através de duas ferramentas para avaliação metodológica. Foram realizadas buscas nos periódicos Evidence-Based Dentistry, Journal of Evidence-Based Dental Practice e no site do Centro de Odontologia Baseada em Evidências da American Dental Associaton. Buscou-se RS nas bases da Biblioteca Cochrane e PubMed/Medline. As palavras selecionas para as buscas foram "motivational interviewing" e "dental caries". Resultados: a busca final resultou em 2 RS sobre o tema. Uma RS de 2020 mostrou redução de cárie através da meta-análise, porém sem diferença estatisticamente significativa; os autores destacam que as evidências limitadas se devem a presença de estudos heterogêneos baseados em diferentes metodologias. Outra RS de 2021 concluiu que a EM possui o potencial de modificar o comportamento e reduzir significativamente lesões cariosas principalmente em crianças com elevada experiência de cárie. Foi ainda avaliada a qualidade metodológica das RS incluídas nesta revisão através dos protocolos PRISMA e AMSTAR-2. Ambas apresentaram baixo risco de viés e alta qualidade. Conclusão: a prática da EM contribui na prevenção da cárie em pré-escolares, principalmente nas crianças de alto risco. Entretanto, as evidências são moderadas, obtidas a partir de meta-análises pouco robustas e de um número limitado de estudos randomizados de boa qualidade metodológica

Aim: to perform an integrative review based on a clinical question: "Can motivational interviewing (MI) be used to prevent dental caries in preschool children?". Materials and methods: an integrative literature review was conducted in April 2021 using an adaptation of the "6S System". We also evaluated the quality of the systematic reviews (SR) found through two tools for methodological assessment. Evidence-Based Dentistry, Journal of Evidence-Based Dental Practice and on the website of the Center for Evidence-Based Dentistry of the American Dental Association. SRs were searched in the Cochrane Library and PubMed/Medline databases were searched. The selected search words were "motivational interviewing" and "dental caries". Results: the final search resulted in 2 SR on the topic. One SR from 2020 showed a reduction in dental caries through meta-analysis, but with no statistically significant difference; the authors point out that the limited evidence is due to the presence of heterogeneous studies based on different methodologies. Another SR from 2021 concluded that MI has the potential to modify behavior and significantly reduce carious lesions, mainly in children with high caries experience. The methodological quality of the SR included in this review was also assessed using the PRISMA and AMSTAR-2 protocols. Both showed high quality and low risk of bias. Conclusion: the practice of MI contributes to the prevention of dental caries in preschoolers, especially in high-risk children. However, the evidence is moderate, obtained from poor meta-analyses and a limited number of randomized studies of good methodological quality.

Arthrocentesis versus nonsurgical methods in the management of temporomandibular joint closed lock and pain: a double-blind randomized controlled trial.

OBJECTIVE: The present double-blind randomized clinical trial aimed to compare the efficacy of conservative treatment and articular lavage, either alone or combined, to reduce joint pain and improve mandibular opening. STUDY DESIGN: The sample consisted of patients presenting with limited mouth opening and joint pain. The diagnosis was made according to the diagnostic criteria for temporomandibular disorders guideline and confirmed by magnetic resonance imaging. Sixty patients were selected and randomly allocated to 4 groups of 15 patients each with different treatments: group A (conservative), group B (conservative + medication), group C (arthrocentesis), and group D (arthrocentesis + medication). The groups were compared in terms of maximal interincisal opening and pain. RESULTS: The average age of the patients was 34.17 ± 13.1 years, 88.1% were women, 72.9% had internal derangement, 54% had joint sounds, and 55.9% presented with locking. Clinical improvement was noted in all parameters compared with baseline in all groups (P < .005), but no significant differences were observed when the groups were compared (P > .05). CONCLUSIONS: Both arthrocentesis and conservative modalities were efficient treatments to reduce joint pain and increase mandibular opening.

Management of Complications Related to Removal of the Buccal Fat Pad.

ABSTRACT: Removal of the buccal fat pad (BFP) is an important topic of discussion in the literature. Several studies have reported improvements in facial esthetics as a result of this technique. The BFP is close to vital structures, such as the facial nerve, parotid duct, and vessels. Injuries related to these structures may occur during the surgical procedure. This manuscript aimed to report and discuss 2 clinical cases of the complications after removal of the BFP. Besides the case presentation, a comprehensive review of the literature was also provided. The reported cases were 2 patients aged 31 and 38 years who were attended by the oral and maxillofacial surgery teams after a complication in the BFP surgery. The first case involved swelling due to Stensen's duct injury, and the second involved uncontrolled bleeding from the internal maxillary artery. Removal of the BFP must have precise indications. Complications may occur during or after surgery; hence, anatomical knowledge is fundamental to appropriate patient management.

Three-dimensional accuracy of virtual planning in orthognathic surgery.

INTRODUCTION: This study aimed to assess the accuracy of virtual surgical planning (VSP) performed by Dolphin Imaging software (version 11.9; Dolphin Imaging and Management Solutions, Chatsworth, Calif). METHODS: Ten people requiring bimaxillary surgery and genioplasty were followed up prospectively. All patients had preoperative cone-beam computed tomography, plaster models, and photographs allowing for VSP. Interocclusal intermediate surgical splints were produced using a 3-dimensional (3D) printer. Postoperative images were acquired 15 days after surgery using cone-beam computed tomography. ITK-Snap (version 3.6; Cognitica, Philadelphia, Pa) allowed the segmentation of reliable 3D models. Geomagic Qualify 2013 (3D Systems, Rock Hill, SC) and MeshValmet (version 3.0) were used to identify the differences between VSP and actual surgical results through the root mean square values and the 3D translational displacement (3-axes) of the 3D centroid of each model. RESULTS: Discrepancies between the VSP and the actual result were found at the mandible (P = 0.013) and the chin (P = 0.013) when considering the root mean square values. In addition, 3D centroid differences were found in the transverse and sagittal direction of the right ramus (P = 0.034 and P = 0.005, respectively) and the sagittal aspect of the left ramus (P = 0.025). Considering 2 mm as a threshold of clinical relevance, almost all the bone fragments (maxilla, proximal, and distal mandibular segments) were accurately corrected by surgery, although not in the chin. CONCLUSIONS: On the basis of the obtained values, it is possible to consider the Dolphin Imaging software as clinically acceptable for performing virtual orthognathic surgical planning.

Demandas de crianças com necessidades especiais de saúde na atenção primária da cidade do Rio de Janeiro / Demands of children with special health care needs in primary care in Rio de Janeiro

Objective: To analyze the demands of children with special health care needs, which implied some changes in the way of taking care at home. Method:Descriptive research implemented with data from Children with Special Health Care Needs Screener© -Brazilian version, integrated with the tri-centric study (Santa Maria ­RS, Rio de Janeiro-RJ, and Ribeirão Preto ­SP). We interviewed 589 families of children under 12 years old in 12 primary health care units. Data were treated with descriptive statistics. Results:According to the domain of special needs, the children's prevalence was 12,4%, being the chronic respiratory and cutaneous diseases the most predominant. In this group, 63% self-declared black and brown skin and 37% white color; 35,6% received benefits from programs of cash transfer (Bolsa Família Programme and Continuous Cash Benefit); 20,5% would live in a community wherein garbage and sewage were discarded at an open-air, with untreated water. Conclusion:The social conditions of vulnerabilities were added to the clinical one, affecting the way those children were taken care of at home. Knowledge regarding the needs, living conditions, care, and access demands can help restructure services and primary health care welcoming.

Objetivo: Analisar demandas de crianças com necessidades especiais de saúde que implicaram em mudanças na forma de cuidar em casa. Método: Pesquisa descritiva implementada com dados do Children with Special Health Care Neesds Screener© - versão brasileira, integrada ao estudo tricêntrico (Santa Maria ­RS, Rio de Janeiro-RJ e Ribeirão Preto ­SP). Entrevistou-se589 familiares de crianças com menos de 12 anos, em 12 serviços da atenção primária. Dados tratados com estatística descritiva. Resultados: Segundo os domínios de necessidades especiais, a prevalência dessas crianças foi 12,4%, predominando as doenças crônicas respiratórias e tegumentares. Desse grupo, 63% era da raça/cor parda e preta e 37%branca; 35,6%eram beneficiados com programas de transferência de renda (bolsa família e prestação continuada); 20,5% viviam em ambientes cujo lixo e esgoto eram descartados a céu aberto, com água sem tratamento. Conclusão: As condições de vulnerabilidade social se somaram às clínicas que afetaram o modo de as crianças serem cuidadas em casa. Conhecimento sobre as necessidades, condições de vida, demandas de cuidado e de acesso podem auxiliar na reestruturação dos serviços e acolhimento na atenção primária.

Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines.

Medulloblastoma (MB) is the most common malignant brain tumor in children. Recent advances in molecular technologies allowed to classify MB in 4 major molecular subgroups: WNT, SHH, Group 3 and Group 4. In cancer research, cancer cell lines are important for examining and manipulating molecular and cellular process. However, it is important to know the characteristics of each cancer cell line prior to use, because there are some differences among them, even if they originate from the same cancer type. This study aimed to evaluate the similarities and differences among four human medulloblastoma cell lines, UW402, UW473, DAOY and ONS-76. The medulloblastoma cell lines were analyzed for (1) cell morphology, (2) immunophenotyping by flow cytometry for some specifics surface proteins, (3) expression level of adhesion molecules by RT-qPCR, (4) proliferative potential, (5) cell migration, and (6) in vivo tumorigenic potential. It was observed a relationship between cell growth and CDH1 (E-chaderin) adhesion molecule expression and all MB cell lines showed higher levels of CDH2 (N-chaderin) when compared to other adhesion molecule. ONS-76 showed higher gene expression of CDH5 (VE-chaderin) and higher percentage of CD144/VE-chaderin positive cells when compared to other MB cell lines. All MB cell lines showed low percentage of CD34, CD45, CD31, CD133 positive cells and high percentage of CD44, CD105, CD106 and CD29 positive cells. The DAOY cell line showed the highest migration potential, the ONS-76 cell line showed the highest proliferative potential and only DAOY and ONS-76 cell lines showed tumorigenic potential in vivo. MB cell lines showed functional and molecular differences among them, which it should be considered by the researchers in choosing the most suitable cellular model according to the study proposal.

Intravascular flow detection during transforaminal epidural injections: a prospective assessment.

BACKGROUND: Transforaminal epidural steroid injections (TFESI) are a mainstay in the treatment of spine pain. Though this commonly performed procedure is generally felt to be safe, devastating complications following inadvertent intra-arterial injections of particulate steroid have been reported. The use of digital subtraction angiography (DSA) has been suggested as a means of detecting intra-arterial needle placements prior to medication injection. OBJECTIVE: To examine the efficacy of DSA in detecting intra-arterial needle placements during TFESI. STUDY DESIGN: Prospective cohort study evaluating the impact of DSA on detecting intra-arterial needle placements during TFESI. METHODS: We enrolled 150 consecutive patients presenting to a university-affiliated spine center with discogenic and/or radicular symptoms affecting the cervical, lumbar, and sacral regions. For each injection, prior to imaging with DSA, traditional methods for vascular penetration detection were employed, including the identification of blood in the needle hub (flash), negative aspiration of blood prior to injection, and live fluoroscopic injection of contrast. Once these tests were performed and negative for signs of intra-arterial needle placement, DSA imaging was utilized prior to medication administration for identification of vascular flow. RESULTS: A total number of 222 TFESI were performed, 41 injections at the cervical levels (18.47%), 113 at the lumbar levels (50.9%), and 68 at the sacral levels (30.36%). Flash was observed in 13 injections performed (5.85% of the total number of injections): one (0.45%) in the cervical, 2 (0.9%) in the lumbar, and 10 (4.5%) in the sacral levels. In 11 TFESI blood aspiration was obtained (4.95% of all injections): 3 (1.3%) in cervical, 4 (1.8%) in lumbar, and 4 (1.8%) in sacral injections. Live fluoroscopy during contrast injection detected 46 (20.72%) intravascular flow patterns: 7 (3.1%) cervical, 17 (7.6%) lumbar, and 22 (9.9%) sacral. DSA identified an additional 5 intravascular injections after all previous steps had resulted in negative vascular penetration signs, which accounted for 2.25% of all injections. LIMITATIONS: This is a prospective, single-center study with a relatively small number of patients and no control group. CONCLUSION: DSA detected additional 5.26% intravascular needle placements following traditional methods. Our findings also support other studies that conclude TFESI are generally a safe procedure. We recommend that special attention should be paid to the sacral injections as vascular penetration was statistically higher than at other levels.

Cytokinesis-block micronucleus assay adapted for analyzing genomic instability of human mesenchymal stem cells.

Human mesenchymal stem cells (hMSCs) are multipotent cells used in cell therapy research. One of the problems involving hMSCs is the possibility of genetic instability during in vitro expansion required to obtain a suitable number of cells for clinical applications. The cytokinesis-block micronucleus (CBMN) assay measures genetic instability by analyzing the presence of micronucleus (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs) in binucleated cells. The present study describes modifications in the CBMN assay methodology to analyze genetic instability in hMSCs isolated from the umbilical vein and in vitro expanded. The best protocol to achieve binucleated hMSCs with preserved cytoplasm was as follows: cytochalasin B concentration (4.0 µg/mL), use of hypotonic treatment (3 min), and the fixative solution (9 methanol:1 acetic acid). These adaptations were reproduced in three hMSC primary cell cultures and also in XP4PA and A549 cell lines. The frequency of hMSCs treated with mitomycin-C presenting MN was lower than that with other nuclear alterations, indicating that the hMSCs contain mechanisms to avoid a high level of chromosomal breaks. However, a high frequency of cells with NPBs was detected and spontaneous anaphase bridges under normal hMSC in vitro culture were observed. Considering that anaphase bridges are characteristic alterations in tumor cells, the CBMN assay is indicated as an important tool associated with other genetic analyses in order to ensure the safe clinical use of hMSCs in cell therapy.

Assessment and treatment of pain with non-invasive cortical stimulation.

PURPOSE: There remains an unmet clinical need for the development of new therapeutic approaches for the treatment of pain. Recent findings have confirmed significant changes in the pain-related neural networks among patients with chronic pain, opening novel possibilities for investigation. Two non-invasive techniques (transcranial magnetic stimulation (TMS) and transcranial direct current stimulation (tDCS)) have emerged as interesting, effective, and promising modalities for pain relief. METHODS: Here we review the clinical efficacy of these techniques for the treatment of pain through an updated systematic meta-analysis on the effects of primary motor cortex stimulation on pain and we discuss potential mechanisms of action based on insights from brain stimulation studies. Our meta-analysis includes 18 studies, which together show that non-invasive brain stimulation is associated with an effect size of -0.86 (95% C.I., -1.54, -0.19) on a standardized pain scale ranging from 0 (no pain) to 10 (worst pain possible). RESULTS AND CONCLUSIONS: Besides its use as a therapeutic tool, non-invasive brain stimulation can also be used to measure cortical reactivity and plasticity in chronic pain. Such measurements could potentially be used as biomarkers for the dysfunctional chronic pain-related neural network and might be helpful in measuring the efficacy of interventions designed for chronic pain.

Sample size calculation in physical medicine and rehabilitation: a systematic review of reporting, characteristics, and results in randomized controlled trials.

OBJECTIVE: To assess systematically the reporting of sample size calculation in randomized controlled trials (RCTs) in 5 leading journals in the field of physical medicine and rehabilitation (PM&R). DATA SOURCES: The data source was full reports of RCTs in 5 leading PM&R journals (Journal of Rehabilitation Medicine, Archives of Physical Medicine and Rehabilitation, American Journal of Physical Medicine and Rehabilitation, Clinical Rehabilitation, and Disability and Rehabilitation) between January and December of 1998 and 2008. Articles were identified in Medline. STUDY SELECTION: A total of 111 articles met our inclusion criteria, which include RCTs of human studies in the 5 selected journals. DATA EXTRACTION: Sample size calculation reporting and trial characteristics were collected for each trial by independent investigators. DATA SYNTHESIS: In 2008, 57.3% of articles reported sample size calculation as compared with only 3.4% in 1998. The parameters that were commonly used were a power of 80% and alpha of 5%. Articles often failed to report effect size or effect estimates for sample size calculation. Studies reporting sample size calculation were more likely to describe the main outcome and to have a sample size greater than 50 subjects. The study outcome (positive vs negative) was not associated with the likelihood of sample size reporting. Trial characteristics of the 2 periods (1998 vs 2008) were similar except that in 1998 there were more negative studies compared with 2008. CONCLUSIONS: Although sample size calculation reporting has improved dramatically in 10 years and is comparable with other fields in medicine, it is still not adequate given current publication guidelines.