Global RNAseq of ocular cells reveals gene dysregulation in both asymptomatic and with Congenital Zika Syndrome infants exposed prenatally to Zika virus.

In 2015, Brazil reported an outbreak identified as Zika virus (ZIKV) infection associated with congenital abnormalities. To date, a total of 86 countries and territories have described evidence of Zika infection and recently the appearance of the African ZIKV lineage in Brazil highlights the risk of a new epidemic. The spectrum of ZIKV infection-induced alterations at both cellular and molecular levels is not completely elucidated. Here, we present for the first time the gene expression responses associated with prenatal ZIKV infection from ocular cells. We applied a recently developed non-invasive method (impression cytology) which use eye cells as a model for ZIKV studies. The ocular profiling revealed significant differences between exposed and control groups, as well as a different pattern in ocular transcripts from Congenital Zika Syndrome (CZS) compared to ZIKV-exposed but asymptomatic infants. Our data showed pathways related to mismatch repair, cancer, and PI3K/AKT/mTOR signaling and genes probably causative or protective in the modulation of ZIKV infection. Ocular cells revealed the effects of ZIKV infection on primordial neuronal cell genes, evidenced by changes in genes associated with embryonic cells. The changes in gene expression support an association with the gestational period of the infection and provide evidence for the resulting clinical and ophthalmological pathologies. Additionally, the findings of cell death- and cancer-associated deregulated genes raise concerns about the early onset of other potential pathologies including the need for tumor surveillance. Our results thus provide direct evidence that infants exposed prenatally to the Zika virus, not only with CZS but also without clinical signs (asymptomatic) express cellular and molecular changes with potential clinical implications.

Outcomes for Latin American versus White patients suffering from acute mania in a randomized, double-blind trial comparing olanzapine and haloperidol.

Data from a published double-blind randomized trial comparing olanzapine versus haloperidol in acute mania were used to address the response and tolerability of Latin American patients. Primary efficacy end point was the remission rate (Young Mania Rating Scale score

Revascularizaçäo do miocárdio sem circulaçäo extracorpórea com derivaçäo intraluminal temporária (DILT) na emergência pós angioplastia transluminal coronária (ATC) / Myocardial revascularization without cardiopulmonary bypass with temporary intraluminal shunt: emergential approach after transluminal coronary angioplasty

No período de novembro 1989 a dezembro 1995, 18 doentes foram submetidos a cirurgia de emergência para revascularizaçao do miocárdio sem circulaçao extracorpórea (CEC), devido a insucesso de angioplastia transluminal percutânea (ATC). Todos os pacientes apresentavam lesoes do ramo interventricular anterior (RIA), coronária direita (CD) ou coronária diagonal (Dg), passíveis de abordagem sem CEC. Em todos utilizamos derivaçao intraluminal temporária (DITL) para manter o fluxo coronariano e minimizar a isquemia. Três (l6,66 por cento) doentes foram operados na vigência de infarto agudo do miocárdio e 4 (22,22 por cento) doentes com importante corrente de lesao ao ECG. O choque cardiogênico estava presente em 3 (l6,66 por cento) doentes. Nao houve mortalidade. Realizamos estudo estatístico para comparaçao da mortalidade referida por diversos autores utilizando revascularizaçao com CEC.