Liver transcriptome and proteome are modulated by nutrient restriction in early lactation cows challenged with intramammary lipopolysaccharide.

The objective was to evaluate the effects of nutrient restriction on liver function 24 h after an intramammary lipopolysaccharide (LPS) challenge in early lactation cows using transcriptomic and proteomic analyses. Multiparous Holstein cows were fed a lactation diet (CONT, n = 8) throughout the study or were switched to a diet diluted with barley straw (48 % DM) for 96 h (REST, n = 8) starting at 24 (18 to 30) days in milk. At 72 h, a healthy rear mammary quarter was infused with 50 µg of LPS in all cows. Blood and liver biopsies were collected at 96 h, corresponding to 24 h after LPS challenge. Liver transcriptome was analyzed with a 44 K bovine microarray and proteome by LC MS/MS. Transcriptomic and proteomic data were analyzed using GeneSpring (moderated t-test with Westfall-Young correction) and the "between subject design", respectively. Data mining was performed using Panther and Pathway Studio software. By design, the negative energy balance was -68 and -37 MJ/d in REST and CONT, respectively. Plasma non-esterified FAs, and ß-hydroxybutyrate were significantly greater in REST compared to CONT, which is consistent with 96 h of nutrient restriction in REST and ketosis induction. We detected 77 and 91 differentially expressed genes at mRNA and protein levels, respectively, between CONT and REST. Genes involved in fatty acid synthesis (e.g.: ACAT, FASN, SCD) were downregulated in REST, whereas those involved in fatty acid oxidation, detoxification, cholesterol synthesis, lipoprotein lipid secretion, and gluconeogenesis (e.g.: ACAD, CPT1A, CPT1B, CPT2) were upregulated. Differentially abundant mRNAs and proteins were consistent with negative energy balance and plasma metabolite concentrations, and reflected a state of intense lipomobilization, glucose deficit and ketogenesis in REST cows. Nutrient restriction did not change in deep liver expression of genes directly involved in immune function 24 h after an intramammary LPS challenge.

Longitudinal associations in dementia family caregivers of ambivalent feelings and disruptive behaviors with C-reactive protein, interleukin-6, and D-dimer.

OBJECTIVE: Caregivers' ambivalent feelings toward the care recipient have been found to be associated with depression and anxiety. There is no research linking caregivers' ambivalent feelings and cardiovascular risk. This study was aimed to analyze longitudinally the effect of ambivalence on caregivers' cardiovascular risk, defined by circulating levels of high-sensitivity C-reactive protein, interleukin-6 (IL-6), and D-dimer. METHOD: Participants were 121 dementia family caregivers who were assessed three times during a 2-year period. Sociodemographic and health variables, behavioral and psychological symptoms of dementia (BPSD), ambivalent feelings, and C-reactive protein (CRP), IL-6, and D-dimer values were assessed. Mixed linear models were used to analyze the association between variables, including testing whether ambivalent feelings moderated the links between BPSD and biomarkers. RESULTS: Increases over time in D-dimer were associated with increases in ambivalence, older age, female gender, and body mass index (BMI). Increases over time in CRP were associated with increases in BMI, older age, female gender, and the interaction of BPSD with caregivers' ambivalent feelings. The moderation analysis showed that increased BPSD was significantly associated with increased CRP when caregivers experienced high levels of ambivalence (p = .006). In contrast, BPSD were not significantly associated with CRP when caregivers experienced low levels of ambivalence (p = .73). Increases in IL-6 were associated with female gender and BMI. The tested model explained 42.85%, 33.15%, and 5.36% of longitudinal variance in CRP, D-dimer, and IL-6 levels, respectively. CONCLUSION: The findings suggest that high ambivalent feelings are relevant for understanding cardiovascular vulnerability in dementia caregivers. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Surfactant-Mediated Microalgal Flocculation: Process Efficiency and Kinetic Modelling.

Microalgae are a valuable source of lipids, proteins, and pigments, but there are challenges in large-scale production, especially in harvesting. Existing methods lack proven efficacy and cost-effectiveness. However, flocculation, an energy-efficient technique, is emerging as a promising solution. Integrating surfactants enhances microalgal harvesting and disruption simultaneously, reducing processing costs. This study investigated cetyltrimethylammonium bromide (CTAB), dodecyltrimethylammonium bromide (DTAB), and sodium dodecyl sulphate (SDS) for harvesting Tetraselmis sp. strains (75LG and 46NLG). CTAB exhibits superior results, with 88% harvesting efficiency at 1500 and 2000 mg L-1 for 75LG and 46NLG, respectively, for 60 min of sedimentation-thus being able to reduce the operating time. Beyond evaluating harvesting efficiency, our study explored the kinetics of the process; the modified Gompertz model led to the best fit. Furthermore, the largest kinetic constants were observed with CTAB, thus highlighting its efficacy in optimising the microalgal harvesting process. With the incorporation of the suggested enhancements, which should be addressed in future work, CTAB could hold the potential to optimise microalgal harvesting for cost-effective and sustainable large-scale production, eventually unlocking the commercial potential of microalgae for biodiesel production.

Native Microalgae-Bacteria Consortia: A Sustainable Approach for Effective Urban Wastewater Bioremediation and Disinfection.

Urban wastewater is a significant by-product of human activities. Conventional urban wastewater treatment plants have limitations in their treatment, mainly concerning the low removal efficiency of conventional and emerging contaminants. Discharged wastewater also contains harmful microorganisms, posing risks to public health, especially by spreading antibiotic-resistant bacteria and genes. Therefore, this study assesses the potential of a native microalgae-bacteria system (MBS) for urban wastewater bioremediation and disinfection, targeting NH4+-N and PO43--P removal, coliform reduction, and antibiotic resistance gene mitigation. The MBS showed promising results, including a high specific growth rate (0.651 ± 0.155 d-1) and a significant average removal rate of NH4+-N and PO43--P (9.05 ± 1.24 mg L-1 d-1 and 0.79 ± 0.06 mg L-1 d-1, respectively). Microalgae-induced pH increase rapidly reduces coliforms (r > 0.9), including Escherichia coli, within 3 to 6 days. Notably, the prevalence of intI1 and the antibiotic resistance genes sul1 and blaTEM are significantly diminished, presenting the MBS as a sustainable approach for tertiary wastewater treatment to combat eutrophication and reduce waterborne disease risks and antibiotic resistance spread.

Longitudinal effects of ambivalent and guilt feelings on dementia family caregivers' depressive symptoms.

BACKGROUND: The world prevalence of people with dementia is increasing. Most of the care received by people with dementia is provided by family caregivers, and this prolonged activity has a significant impact on caregivers' levels of depression. Stressors and frequency of leisure are known predictors of caregivers' depressive levels. The longitudinal impact of caregivers' ambivalent and guilt feelings is unknown. METHODS: Participants were 177 family caregivers of relatives with dementia who were assessed three times during a 2-year period. In addition to demographic variables, psychological symptoms of the dementias, and frequency of leisure activities, caregivers' ambivalent feelings, guilt, and depressive symptoms were measured. The longitudinal association of changes in these variables with changes in caregivers' depressive symptoms over time was assessed using mixed linear models. RESULTS: Changes over time in the assessed variables predicted 48.05% of variance of changes over time in depressive symptoms. Even when variables strongly associated with increased depressive symptoms were controlled (lower caregivers' age and educational level, higher reaction to BPSD, and lower leisure activities), increases in ambivalence and guilt contributed to an increase of 9.22% of the variance of changes depressive symptoms over a 2-year period. The effects of ambivalent feelings on depression are indirect, mediated by guilt feelings. Cessation of caregiving do not seem to alter these findings. CONCLUSIONS: Caregivers' ambivalent and guilt feelings are significant predictors of caregivers' mental health. Caregivers may significantly benefit from early detection of ambivalent and guilt feelings and preventive strategies targeting triggers associated with ambivalent and guilt symptoms.

Microalgae-Based Remediation of Real Textile Wastewater: Assessing Pollutant Removal and Biomass Valorisation.

The textile industry generates highly contaminated wastewater. It severely threatens local ecosystems without proper treatment, significantly diminishing biodiversity near the discharge point. With rapid growth rates, microalgae offer an effective solution to mitigate the environmental impact of textile wastewater, and the generated biomass can be valorised. This study sets out to achieve two primary objectives: (i) to assess the removal of pollutants by Chlorella vulgaris from two distinct real textile wastewaters (without dilution) and (ii) to evaluate microalgal biomass composition for further valorisation (in a circular economy approach). Microalgae grew successfully with growth rates ranging from 0.234 ± 0.005 to 0.290 ± 0.003 d-1 and average productivities ranging from 78 ± 3 to 112.39 ± 0.07 mgDW L-1 d-1. All cultures demonstrated a significant reduction in nutrient concentrations for values below the legal limits for discharge, except for COD in effluent 2. Furthermore, the pigment concentration in the culture increased during textile effluent treatment, presenting a distinct advantage over conventional ones due to the economic value of produced biomass and pigments. This study underscores the promise of microalgae in textile wastewater treatment and provides valuable insights into their role in addressing the environmental challenges the textile industry poses.

Validation of the Spanish version of the Perceived Control Scale: Perceived Constraints on Personal Control and Perceived Mastery.

OBJECTIVES: Perceived control is a relevant variable for understanding middle and older adults' mental health. The Perceived Control Scale has two dimensions, Perceived Constraints on Personal Control and Perceived Mastery, and is a widely used instrument for assessing perceived control in adults. The aim of this study was to analyze the psychometric properties of the Spanish version of the Perceived Control Scale with data from two different studies. METHOD: In Study 1, 348 older adults between 60 and 92 years old were assessed through face-to-face assessments. In Study 2, 334 adults between 40 and 90 years completed an online survey. Perceived control, self-perceptions of aging, and anxiety and depressive symptomatology were assessed. An exploratory (Study 1) and confirmatory (Study 2) factor analysis of the Perceived Control Scale were performed, and reliability and its relationship with the other assessed variables were evaluated. RESULTS: Results from parallel, exploratory, and confirmatory factor analyses suggested that the Spanish version of the Perceived Control Scale has a bidimensional structure: Perceived Constraints on Personal Control and Perceived Mastery. Good internal consistency was found for the scale in both samples. The results revealed negative and significant associations with negative self-perceptions of aging, and anxiety and depressive symptomatology. CONCLUSION: The Spanish version of the Perceived Control Scale shows good psychometric properties which endorse its use with middle-aged and older adults assessed either face-to-face or through online surveys.

Comparative assessment of microalgal growth kinetic models based on light intensity and biomass concentration.

The comprehensive evaluation and validation of mathematical models for microalgal growth dynamics are essential for improving cultivation efficiency and optimising photobioreactor design. A considerable gap in comprehending the relation between microalgal growth, light intensity and biomass concentration arises since many studies focus solely on associating one of these factors. This paper compares microalgal growth kinetic models, specifically focusing on the combined impact of light intensity and biomass concentration. Considering a dataset (experimental results and literature values) concerning Chlorella vulgaris, nine kinetic models were assessed. Bannister and Grima models presented the best fitting performance to experimental data (RMSE ≤ 0.050 d-1; R2≥0.804; d2≥0.943). Cultivation conditions conducting photoinhibition were identified in some kinetic models. After testing these models on independent datasets, Bannister and Grima models presented superior predictive performance (RMSE = 0.022-0.023 d-1; R2 = 0.878-0.884; d2: 0.976-0.975). The models provide valuable tools for predicting microalgal growth and optimising operational parameters, reducing the need for time-consuming and costly experiments.

Milk fat miRNome changes in response to LPS challenge in Holstein cows.

Mastitis is an inflammatory disease in dairy cows, causing economic losses and reducing animal welfare. In order to contribute for the discovery of early and noninvasive indicators, our objective was to determine the effects of a lipopolysaccharide (LPS) challenge on the microRNA profile (miRNome) of milk fat, using microarray analyses in cows. Cows were fed a lactation diet at ad libitum intake (n = 6). At 27 ± 3 days in milk, cows were injected with 50 µg of LPS Escherichia coli in one healthy rear mammary quarter. Milk samples were collected just before LPS challenge (LPS-) and 6.5 h after LPS challenge (LPS +) from the same cows. Microarray analysis was performed using customized 8 × 60 K ruminant miRNA microarrays to compare LPS- to LPS + miRNome. In silico functional analyses were performed using OmicsNet and Mienturnet software. MiRNome comparison between LPS- and LPS + identified 37 differentially abundant miRNAs (q-value ≤ 0.05). The predicted target genes of the 37 differentially abundant miRNAs are mostly involved in cell life including apoptosis, cell cycle, proliferation and differentiation and in gene expression processes. MiRNome analyses suggest that miRNAs profile is related to the inflammation response of the mammary gland. In conclusion, we demonstrated that milk fat might be an easy and rapid source of miRNAs that are potential indicators of early mastitis in cows.

Hypertonic glucose in the treatment of low back pain: A randomized clinical trial.

BACKGROUND: Chronic low back pain (LBP) is defined as pain lasting longer than 3 months and is one of the conditions with the most significant social impact. Treatment is complex and includes proliferative agents used in prolotherapy. The mechanism is not known, but osmotic agents (hypertonic solutions of dextrose or glucose) cause cellular rupture and an inflammatory response that releases cytokines and growth factors that lead to scarring and reinnervation. METHODS: Patients with chronic LBP (>12 weeks) who were non-traumatic and unresponsive to at least 1 month of physical therapy were selected. All patients were followed up at a return visit at 1, 3, and 6 months for pain assessment using validated pain questionnaires and scales. RESULTS: Nineteen patients were included in the conservative group and 19 in the glucose group, with the majority being women (57.9%). The patients were between 47 and 59 years of age (39.5%), mixed race (76.33%), married or in a committed relationship (73.7%), and had completed the study for 5.2 years. Overall mean body mass index was 27.3 ± 4.4 kg/m 2 and was higher in the conservative group (28.0 ± 4.7 kg/m 2 ). The groups showed differences in Visual Analog Scale scores, with median and amplitude values close to each other between the time points evaluated and increasing values in the glucose group, which had significantly higher values for this scale at the third evaluation ( P = .031). When comparing the Rolland-Morris scale scores between the groups, there was a significant difference only in the 3-m assessment ( P = .021). In the follow-up assessment, both groups showed significant improvement between T0 and the other assessment time points ( P < .05) in all evaluations. CONCLUSION: Both groups significantly improved on the evaluated scales during follow-up. Overall, no effects were attributable to the glucose components or the prolotherapy protocol.

Cross-National Analysis of the Associations Between Familism and Self-Efficacy in Family Caregivers of People With Dementia: Effects on Burden and Depression.

Objectives: To examine the cross-national associations between familism and self-efficacy dimensions, and levels of burden and depression. Methods: Sociodemographic, familism, self-efficacy, depressive symptoms, and burden variables were measured in 349 dementia family caregivers from the US and Spain. Results: US sample: greater support from family was positively related to self-efficacy for obtaining respite and self-efficacy for controlling upsetting thoughts and behaviors. Both self-efficacy constructs were negatively related to depression. Similar findings were obtained for burden. Spanish sample: higher scores on family as referents were associated with lower scores on self-efficacy for obtaining respite; lower scores on self-efficacy for obtaining respite were associated with higher depressive symptomatology. Discussion: Study findings suggest that a significant interplay exists between the various facets of familism and self-efficacy, leading to differential caregiving outcomes. Unique cultural contexts and values derived from each country may exert distinct influences on how the caregiving role is perceived and appraised.

Microalgae to Bioenergy: Optimization of Aurantiochytrium sp. Saccharification.

Microalgae are a promising feedstock for bioethanol production, essentially due to their high growth rates and absence of lignin. Hydrolysis-where the monosaccharides are released for further fermentation-is considered a critical step, and its optimization is advised for each raw material. The present study focuses on the thermal acid hydrolysis (with sulfuric acid) of Aurantiochytrium sp. through a response surface methodology (RSM), studying the effect of acid concentration, hydrolysis time and biomass/acid ratio on both sugar concentration of the hydrolysate and biomass conversion yield. Preliminary studies allowed to establish the range of the variables to be optimized. The obtained models predicted a maximum sugar concentration (18.05 g/L; R2 = 0.990) after 90 min of hydrolysis, using 15% (w/v) biomass/acid ratio and sulfuric acid at 3.5% (v/v), whereas the maximum conversion yield (12.86 g/100 g; R2 = 0.876) was obtained using 9.3% (w/v) biomass/acid ratio, maintaining the other parameters. Model outputs indicate that the biomass/acid ratio and time are the most influential parameters on the sugar concentration and yield models, respectively. The study allowed to obtain a predictive model that is very well adjusted to the experimental data to find the best saccharification conditions for the Aurantiochytrium sp. microalgae.

Marital Satisfaction and Mental Health in Adults Over 40 Years Old. Associations with Self-Perceptions of Aging and Stress Related to the COVID–19 Pandemic

Being married has been associated with a better attitude to aging and a buffer against stressful situations, factors that influence mental health. The study analyzes the role of self-perceptions of aging and stress related to the COVID–19 pandemic in the association between marital satisfaction and participants’ mental health. 246 people older than 40 years in a marital/partner relationship were assessed. A path analysis was tested, where self-perceptions of aging and stress from the COVID–19 situation were proposed as mechanisms of action in the association between marital satisfaction and anxious and depressive symptoms. Marital satisfaction, self-perceptions of aging, and stress associated with the COVID–19 pandemic significantly contributed to the model and explained 31% of the variance in participants´ anxious symptomatology, and 42% of the variance in depressive symptomatology. The indirect path of self-perceptions of aging and stress associated with the COVID–19 pandemic in the link between marital satisfaction and anxious and depressive symptoms was statistically significant for both outcome variables. The findings of this study suggest that lower perceived marital satisfaction is associated with higher levels of negative self-perceptions of aging and with higher anxiety and depressive symptoms. Public significance statements: This study suggests that higher marital satisfaction may be a buffer for negative self-perception of aging, and both factors are related with experiencing less stress from COVID–19. These links are associated with less anxious and depressive symptoms. (AU)

Differential Protein and Glycan Packaging into Extracellular Vesicles in Response to 3D Gastric Cancer Cellular Organization.

Alterations of the glycosylation machinery are common events in cancer, leading to the synthesis of aberrant glycan structures by tumor cells. Extracellular vesicles (EVs) play a modulatory role in cancer communication and progression, and interestingly, several tumor-associated glycans have already been identified in cancer EVs. Nevertheless, the impact of 3D tumor architecture in the selective packaging of cellular glycans into EVs has never been addressed. In this work, the capacity of gastric cancer cell lines with differential glycosylation is evaluated in producing and releasing EVs when cultured under conventional 2D monolayer or in 3D culture conditions. Furthermore, the proteomic content is identified and specific glycans are studied in the EVs produced by these cells, upon differential spatial organization. Here, it is observed that although the proteome of the analyzed EVs is mostly conserved, an EV differential packaging of specific proteins and glycans is found. In addition, protein-protein interaction and pathway analysis reveal individual signatures on the EVs released by 2D- and 3D-cultured cells, suggesting distinct biological functions. These protein signatures also show a correlation with clinical data. Overall, this data highlight the importance of tumor cellular architecture when assessing the cancer-EV cargo and its biological role.

Microalgal cultures for the remediation of wastewaters with different nitrogen to phosphorus ratios: Process modelling using artificial neural networks.

Microalgae have remarkable potential for wastewater bioremediation since they can efficiently uptake nitrogen and phosphorus in a sustainable and environmentally friendly treatment system. However, wastewater composition greatly depends on its source and has a significant seasonal variability. This study aimed to evaluate the impact of different N:P molar ratios on the growth of Chlorella vulgaris and nutrient removal from synthetic wastewater. Furthermore, artificial neural network (ANN) threshold models, optimised by genetic algorithms (GAs), were used to model biomass productivity (BP) and nitrogen/phosphorus removal rates (RRN/RRP). The impact of various inputs culture variables on these parameters was evaluated. Microalgal growth was not nutrient limited since the average biomass productivities and specific growth rates were similar between the experiments. Nutrient removal efficiencies/rates reached 92.0 ± 0.6%/6.15 ± 0.01 mgN L-1 d-1 for nitrogen and 98.2 ± 0.2%/0.92 ± 0.03 mgP L-1 d-1 for phosphorus. Low nitrogen concentration limited phosphorus uptake for low N:P ratios (e.g., 2 and 3, yielding 36 ± 2 mgDW mgP-1 and 39 ± 3 mgDW mgP-1, respectively), while low phosphorus concentration limited nitrogen uptake with high ratios (e.g., 66 and 67, yielding 9.0 ± 0.4 mgDW mgN-1 and 8.8 ± 0.3 mgDW mgN-1, respectively). ANN models showed a high fitting performance, with coefficients of determination of 0.951, 0.800, and 0.793 for BP, RRN, and RRP, respectively. In summary, this study demonstrated that microalgae could successfully grow and adapt to N:P molar ratios between 2 and 67, but the nutrient uptake was impacted by these variations, especially for the lowest and highest N:P molar ratios. Furthermore, GA-ANN models demonstrated to be relevant tools for microalgal growth modelling and control. Their high fitting performance in characterising this biological system can contribute to reducing the experimental effort for culture monitoring (human resources and consumables), thus decreasing the costs of microalgae production.

Marital Satisfaction and Mental Health in Adults Over 40 Years Old. Associations with Self-Perceptions of Aging and Stress Related to the COVID-19 Pandemic.

Being married has been associated with a better attitude to aging and a buffer against stressful situations, factors that influence mental health. The study analyzes the role of self-perceptions of aging and stress related to the COVID-19 pandemic in the association between marital satisfaction and participants' mental health. 246 people older than 40 years in a marital/partner relationship were assessed. A path analysis was tested, where self-perceptions of aging and stress from the COVID-19 situation were proposed as mechanisms of action in the association between marital satisfaction and anxious and depressive symptoms. Marital satisfaction, self-perceptions of aging, and stress associated with the COVID-19 pandemic significantly contributed to the model and explained 31% of the variance in participants´ anxious symptomatology, and 42% of the variance in depressive symptomatology. The indirect path of self-perceptions of aging and stress associated with the COVID-19 pandemic in the link between marital satisfaction and anxious and depressive symptoms was statistically significant for both outcome variables. The findings of this study suggest that lower perceived marital satisfaction is associated with higher levels of negative self-perceptions of aging and with higher anxiety and depressive symptoms. Public significance statements: This study suggests that higher marital satisfaction may be a buffer for negative self-perception of aging, and both factors are related with experiencing less stress from COVID-19. These links are associated with less anxious and depressive symptoms.

Microalgae systems - environmental agents for wastewater treatment and further potential biomass valorisation.

Water is the most valuable resource on the planet. However, massive anthropogenic activities generate threatening levels of biological, organic, and inorganic pollutants that are not efficiently removed in conventional wastewater treatment systems. High levels of conventional pollutants (carbon, nitrogen, and phosphorus), emerging chemical contaminants such as antibiotics, and pathogens (namely antibiotic-resistant ones and related genes) jeopardize ecosystems and human health. Conventional wastewater treatment systems entail several environmental issues: (i) high energy consumption; (ii) high CO2 emissions; and (iii) the use of chemicals or the generation of harmful by-products. Hence, the use of microalgal systems (entailing one or several microalgae species, and in consortium with bacteria) as environmental agents towards wastewater treatment has been seen as an environmentally friendly solution to remove conventional pollutants, antibiotics, coliforms and antibiotic resistance genes. In recent years, several authors have evaluated the use of microalgal systems for the treatment of different types of wastewater, such as agricultural, municipal, and industrial. Generally, microalgal systems can provide high removal efficiencies of: (i) conventional pollutants, up to 99%, 99%, and 90% of total nitrogen, total phosphorus, and/or organic carbon, respectively, through uptake mechanisms, and (ii) antibiotics frequently found in wastewaters, such as sulfamethoxazole, ciprofloxacin, trimethoprim and azithromycin at 86%, 65%, 42% and 93%, respectively, through the most desirable microalgal mechanism, biodegradation. Although pathogens removal by microalgal species is complex and very strain-specific, it is also possible to attain total coliform and Escherichia coli removal of 99.4% and 98.6%, respectively. However, microalgal systems' effectiveness strongly relies on biotic and abiotic conditions, thus the selection of operational conditions is critical. While the combination of selected species (microalgae and bacteria), ratios and inoculum concentration allow the efficient removal of conventional pollutants and generation of high amounts of biomass (that can be further converted into valuable products such as biofuels and biofertilisers), abiotic factors such as pH, hydraulic retention time, light intensity and CO2/O2 supply also have a crucial role in conventional pollutants and antibiotics removal, and wastewater disinfection. However, some rationale must be considered according to the purpose. While alkaline pH induces the hydrolysis of some antibiotics and the removal of faecal coliforms, it also decreases phosphates solubility and induces the formation of ammonium from ammonia. Also, while CO2 supply increases the removal of E. coli and Pseudomonas aeruginosa, as well as the microalgal growth (and thus the conventional pollutants uptake), it decreases Enterococcus faecalis removal. Therefore, this review aims to provide a critical review of recent studies towards the application of microalgal systems for the efficient removal of conventional pollutants, antibiotics, and pathogens; discussing the feasibility, highlighting the advantages and challenges of the implementation of such process, and presenting current case-studies of different applications of microalgal systems.

Family caregivers of people with dementia in the context of the sociocultural stress and coping model: An examination of gender differences.

The study assessed gender differences in the associations between familism dimensions, dysfunctional thoughts, and resource and outcome variables in 190 Spanish dementia family caregivers. A theoretical model was tested through path analysis, obtaining an excellent fit of the model to the data. Higher scores in the familial obligations dimension were associated with lower self-efficacy for self-care and obtaining respite and leisure frequency, and higher anxious and depressive feelings, in female caregivers only. Significant between-gender differences were obtained, suggesting that female caregivers holding strong familistic values are more vulnerable to the negative consequences of caregiving compared to male caregivers.

Cross-cultural analysis of the role of ambivalent feelings for understanding caregivers' depressive symptoms.

OBJECTIVES: Behavioral and psychological symptoms of dementia (BPSD) are considered to cause ambivalent feelings in caregivers that may contribute to understanding their depressive symptoms. Transnational research is needed in order to increase our knowledge about the cross-cultural equivalence of theoretical models to understand caregivers' mental health. The aim of this study was to cross-culturally analyze the association between BPSD, ambivalent feelings and depressive symptoms in two samples of family caregivers of people with dementia from Spain and the UK. METHODS: Participants in this study were 432 caregivers who completed measures of BPSD, ambivalent feelings and depressive symptoms. The association between the assessed variables was tested through path-analysis, with differences between countries tested through multigroup analysis. RESULTS: The results suggest that the influence of BPSD on caregivers' depressive symptoms is indirect, through ambivalent feelings. The observed associations were equivalent between countries and explained a significant percentage of the variance of depressive symptoms. CONCLUSION: The findings of this study provide, for the first time, evidence of equivalent cross-cultural paths analyzing the role of ambivalent feelings for understanding caregivers' depressive symptoms. The practical implications of these results are discussed.

Hiv/aids e sua principal coinfecção-tuberculose: panorama de uma década de um estado do nordeste brasileiro e suas semelhanças à sociodemografia subsaariana / Hiv/aids and its main coinfection-tuberculosis: a decade panorama of a northeastern brazilian state and its similarities to subsaharan sociodemographics / Vih/sida y su principal coinfección-tuberculosis: panorama decenal de un estado del nordeste brasileño y sus similitudes con la sociodemografía subsahariana

Introdução: Entre o início da epidemia do HIV na década de 1980 até 2018, houve cerca de 32 milhões de óbitos e no mundo existem aproximadamente 37,9 milhões de pessoas viviam com HIV e AIDS. Assim, mesmo com advento da TARV, as pessoas vivendo com HIV/AIDS permanecem sujeitas a redução na qualidade de vida, sendo que dentre esses fatores a coinfecção por Tuberculose se demonstra a principal responsável pela alteração de prognóstico. Desta forma o presente estudo busca estabelecer um panorama epidemiológico das pessoas vivendo com HIV/ AIDS e a sua principal coinfecção no Estado do Maranhão. Metodologia: Trata-se de um estudo epidemiológico descritivo retrospectivo, de abordagem quantitativa que apresenta o perfil das pessoas que vivem com HIV/AIDS, bem como, a principal coinfecção, no período de 2009 a 2018 no Maranhão. Resultados: No período de 2009 a 2018 foram notificados 7691 casos de AIDS no Maranhão, sendo a maioria composta por indivíduos de 20 a 49 anos, do sexo masculino, cor parda e com escolaridade entre a 1ª serie e o ensino fundamental completo, via de transmissão heterossexual e com maior tendência a coinfecção por Tuberculose nos últimos anos da amostra e nos casos de uso de TARV. Conclusão: O Panorama obtido propõe uma maior necessidade de intervenção e conscientização da necessidade de códons, além de maior rastreio de indivíduos no uso da TARV. Embora observada forte redução nas notificações em 2018, há a necessidade de cautela, principalmente pelo retrato da epidemia em regiões com similaridade sócio demográfica.

Introduction: Between the beginning of the HIV epidemic in the 1980s until 2018, there were about 32 million deaths and in the world there are approximately 37.9 million people living with HIV and AIDS. Thus, even with the advent of ART, people living with HIV/AIDS remain subject to reduced quality of life, and among these factors, co-infection with tuberculosis proves to be the main responsible for the change in prognosis. Thus, the present study seeks to establish an epidemiological panorama of people living with HIV/AIDS and their main co-infection in the state of Maranhão. Methodology: This is a retrospective descriptive epidemiological study, of quantitative approach that presents the profile of people living with HIV/AIDS, as well as, the main coinfection, in the period from 2009 to 2018 in Maranhão. Results: In the period from 2009 to 2018, 7691 cases of AIDS were notified in Maranhão, being the majority composed of individuals aged 20 to 49 years, male, brown color and with education between 1st grade and complete elementary school, heterosexual transmission route and with a greater tendency to coinfection by Tuberculosis in the last years of the sample and in cases of ART use. Conclusion: The Panorama obtained proposes a greater need for intervention and awareness of the need for codons, in addition to greater screening of individuals on ART use. Although observed a strong reduction in notifications in 2018, there is a need for caution, mainly because of the epidemic's portrayal in regions with socio demographic similarity.

Introducción: Entre el inicio de la epidemia del VIH en la década de 1980 hasta 2018, hubo cerca de 32 millones de muertes y en el mundo hay aproximadamente 37,9 millones de personas que viven con el VIH y el SIDA. Así, incluso con el advenimiento de la terapia antirretroviral, las personas que viven con VIH/SIDA siguen estando sujetas a la reducción de la calidad de vida, y entre estos factores, la coinfección con tuberculosis resulta ser el principal responsable del cambio en el pronóstico. Así, el presente estudio busca establecer un panorama epidemiológico de las personas que viven con VIH/SIDA y sus principales coinfecciones en el estado de Maranhão. Metodología: Se trata de un estudio epidemiológico descriptivo retrospectivo, de abordaje cuantitativo que presenta el perfil de las personas que viven con VIH/SIDA, así como, la principal coinfección, en el período de 2009 a 2018 en Maranhão. Resultados: En el período de 2009 a 2018, fueron notificados 7691 casos de SIDA en Maranhão, siendo la mayoría compuesta por individuos de 20 a 49 años, de sexo masculino, color castaño y con escolaridad entre 1º grado y primaria completa, vía de transmisión heterosexual y con mayor tendencia a la coinfección por Tuberculosis en los últimos años de la muestra y en los casos de uso de TAR. Conclusiones: El Panorama obtenido propone una mayor necesidad de intervención y concienciación de la necesidad de codones, además de un mayor cribado de los individuos en uso de TAR. A pesar de observarse una fuerte reducción de notificaciones en 2018, es necesario ser cautelosos, principalmente por el retrato de la epidemia en regiones con similitud socio demográfica.