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1.
Animals (Basel) ; 13(3)2023 Jan 21.
Artículo en Inglés | MEDLINE | ID: mdl-36766264

RESUMEN

Myeloperoxidase (MPO), as a marker of neutrophil activation, has been associated with equine endometritis. However, in absence of inflammation, MPO is constantly detected in the uterine lumen of estrous mares. The aim of this study was to characterize MPO in the uterus of mares under physiological conditions as a first step to better understand the role of this enzyme in equine reproduction. Total and active MPO concentrations were determined, by ELISA and SIEFED assay, respectively, in low-volume lavages from mares in estrus (n = 26), diestrus (n = 18) and anestrus (n = 8) in absence of endometritis. Immunohistochemical analysis was performed on 21 endometrial biopsies randomly selected: estrus (n = 11), diestrus (n = 6) and anestrus (n = 4). MPO, although mostly enzymatically inactive, was present in highly variable concentrations in uterine lavages in all studied phases, with elevated concentrations in estrus and anestrus, while in diestrus, concentrations were much lower. Intracytoplasmic immunoexpression of MPO was detected in the endometrial epithelial cells, neutrophils and glandular secretions. Maximal expression was observed during estrus in mid and basal glands with a predominant intracytoplasmic apical reinforcement. In diestrus, immunopositive glands were sporadic. In anestrus, only the luminal epithelium showed residual MPO immunostaining. These results confirm a constant presence of MPO in the uterine lumen of mares in absence of inflammation, probably as part of the uterine mucosal immune system, and suggest that endometrial cells are a source of uterine MPO under physiological cyclic conditions.

2.
J Anat ; 229(6): 791-799, 2016 12.
Artículo en Inglés | MEDLINE | ID: mdl-27345299

RESUMEN

In athletic horses, diseases leading to lameness are of great importance due to the loss of performance and the resultant economic concerns. Although stifle lesions are frequent in the hindlimb, due to the large size and complexity of the joint, and although meniscal tears have been identified as the most common soft tissue injuries in this joint, little is known about the mechanism that causes the painful sensation and thus the lameness. The aim of our study was to highlight any peripheral fibres involved in meniscal nociception in five macroscopically sound cranial horns of the equine medial meniscus, which has been one of the most common sites reported for equine meniscal injuries. Immunohistochemical stainings were performed using antibodies against Substance P in order to identify nociceptive fibres; against tyrosine hydroxylase for detecting postganglionic sympathetic fibres; and against glial fibrillary acidic proteins in order to identify Schwann cells. Our work highlights for the first time the presence of nociceptive and sympathetic fibres in equine menisci. They were found in the abaxial part of the cranial horn of the equine medial meniscus. This study suggests that when the abaxial part is injured, the meniscus itself could be the source of pain. These findings could provide a better understanding of the clinical presentation of horses with meniscal injury and contribute towards improving therapeutic strategies to alleviate pain in cases of equine meniscal injury.


Asunto(s)
Meniscos Tibiales/química , Meniscos Tibiales/inervación , Nociceptores/química , Coloración y Etiquetado/métodos , Fibras Simpáticas Posganglionares/química , Animales , Caballos , Meniscos Tibiales/anatomía & histología , Fibras Simpáticas Posganglionares/anatomía & histología
3.
Reprod Fertil Dev ; 28(5): 628-39, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25482458

RESUMEN

When derived from chicken embryos, avian primordial germ cells (PGCs) have been reported to keep their germline-specific properties and proliferative potential even after long-term culture and genetic modifications. Few teams to date have reported such long-term expansion and engineering without differentiation of primary avian PGCs' cultures. We have developed original and robust methods that allow more than 1 year culture, expansion and cryobanking of primary cultures of PGCs without obvious effects on their biological properties, including their ability to colonise the genital ridges. Overall, 38% of embryonic samples gave rise to PGCs lines derived from three commercial layers and two Belgian endangered breeds. The lines kept their proliferative potential and their characteristic PGCs phenotype after 20 months in culture, whether or not interrupted by a cryopreservation step. All the resulting lines appeared devoid of female cells, although initially pooled from male and female embryos. Labelled PGCs from 12 long-term cultured lines colonised the genital ridges of recipient embryos. Thus, this procedure allows derivation, long-term expansion and cryobanking of primary cultures of PGCs without obvious changes to their original characteristics, providing an alternative access to applications in avian biotechnology and preservation of genetic resources.


Asunto(s)
Movimiento Celular , Proliferación Celular , Pollos/fisiología , Criopreservación/veterinaria , Especies en Peligro de Extinción , Células Germinativas/fisiología , Gónadas/embriología , Animales , Biomarcadores/metabolismo , Diferenciación Celular , Linaje de la Célula , Células Cultivadas , Embrión de Pollo , Pollos/genética , Femenino , Células Germinativas/metabolismo , Células Germinativas/trasplante , Masculino , Fenotipo , Análisis para Determinación del Sexo/veterinaria , Factores de Tiempo
4.
Stem Cell Res Ther ; 6: 253, 2015 Dec 18.
Artículo en Inglés | MEDLINE | ID: mdl-26684484

RESUMEN

BACKGROUND: Mesenchymal stem cells (MSCs) harvested from cadaveric tissues represent a promising approach for regenerative medicine. To date, no study has investigated whether viable MSCs could survive in cadaveric tissues from tendon or ligament up to 72 hours of post-mortem. The purpose of the present work was to find out if viable MSCs could survive in cadaveric tissues from adult equine ligaments up to 72 hours of post-mortem, and to assess their ability (i) to remain in an undifferentiated state and (ii) to divide and proliferate in the absence of any specific stimulus. METHODS: MSCs were isolated from equine cadaver (EC) suspensory ligaments within 48-72 hours of post-mortem. They were evaluated for viability, proliferation, capacity for tri-lineage differentiation, expression of cell surface markers (CD90, CD105, CD73, CD45), pluripotent transcription factor (OCT-4), stage-specific embryonic antigen-1 (SSEA-1), neuron-specific class III beta-tubulin (TUJ-1), and glial fibrillary acidic protein (GFAP). As well, they were characterized by transmission electron microscope (TEM). RESULTS: EC-MSCs were successfully isolated and maintained for 20 passages with high cell viability and proliferation. Phase contrast microscopy revealed that cells with fibroblast-like appearance were predominant in the culture. Differentiation assays proved that EC-MSCs are able to differentiate towards mesodermal lineages (osteogenic, adipogenic, chondrogenic). Flow cytometry analysis demonstrated that EC-MSCs expressed CD90, CD105, and CD73, while being negative for the leukocyte common antigen CD45. Immunofluorescence analysis showed a high percentage of positive cells for OCT-4 and SSEA-1. Surprisingly, in absence of any stimuli, some adherent cells closely resembling neuronal and glial morphology were also observed. Interestingly, our results revealed that approximately 15 % of the cell populations were TUJ-1 positive, whereas GFAP expression was detected in only a few cells. Furthermore, TEM analysis confirmed the stemness of EC-MSCs and identified some cells with a typical neuronal morphology. CONCLUSIONS: Our findings raise the prospect that the tissues harvested from equine ligaments up to 72 hours of post-mortem represent an available reservoir of specific stem cells. EC-MSCs could be a promising alternative source for tissue engineering and stem cell therapy in equine medicine.


Asunto(s)
Caballos/anatomía & histología , Ligamentos/citología , Células Madre Mesenquimatosas/citología , Recolección de Tejidos y Órganos/veterinaria , Animales , Biomarcadores/metabolismo , Cadáver , Técnicas de Cultivo de Célula/métodos , Técnicas de Cultivo de Célula/veterinaria , Diferenciación Celular , Proliferación Celular , Separación Celular/métodos , Separación Celular/veterinaria , Proteína Ácida Fibrilar de la Glía/metabolismo , Caballos/metabolismo , Técnicas In Vitro , Ligamentos/metabolismo , Células Madre Mesenquimatosas/metabolismo , Microscopía Electrónica de Transmisión , Factor 3 de Transcripción de Unión a Octámeros/metabolismo , Cambios Post Mortem , Factores de Tiempo , Recolección de Tejidos y Órganos/métodos , Tubulina (Proteína)/metabolismo
5.
Vet Res Commun ; 38(4): 279-85, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25113608

RESUMEN

Astroglial account for the largest glial population in the brain and play a variety of vital functions in the development of the central nervous system (CNS). An immunohistochemical study was performed in 19 ovine foetuses ranging from 2 to 5 months of gestation, one newborn lamb and three adult sheep. Using the anit-glial fibrillary acidic protein (GFAP) marker, several variations were found in the degree of GFAP positive (GFAP+) astrocyte distribution between the different zones in the cerebellum of sheep during brain development. Our study indicates that the first appearance of astrocytes from restricted zones in the cerebellum occurs around the eighth week of gestation. Bergmann cells were found to be present from around the 15th week of gestation onwards. Our findings suggest that the maturation of astrocytes begins in the caudal parts of the cerebellum, developing from their initial ventral regions to spread first to dorsal regions radially within the white matter, then followed by the more rostral parts of the cerebellum. Astrocytes were also found to proliferate in the vermis before appearing in the cerebellar hemispheres.


Asunto(s)
Astrocitos/citología , Astrocitos/metabolismo , Cerebelo/citología , Proteína Ácida Fibrilar de la Glía/metabolismo , Ovinos/embriología , Animales , Recuento de Células , Cerebelo/metabolismo , Inmunohistoquímica
6.
J Neuroimmunol ; 272(1-2): 1-9, 2014 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-24841625

RESUMEN

During preclinical stage of prion diseases, secondary lymphoid organs seem to play an important role in prion amplification prior the invasion of the associated peripheral nervous system. In mice, it was shown that the relative positioning of follicular dendritic cells (FDC) and sympathetic nervous system (SNS) affects the velocity of neuroinvasion following scrapie inoculation. In this study, we checked if scrapie infection, by oral or intraperitoneal route, could influence this neuroimmune interface between FDC and tyrosine hydroxylase (TH) positive neural fibres within Peyer's patches (PP) and spleen of the C57BL/6 mouse strain. We concluded that, in vivo, scrapie 139A and ME7 strains do not modify FDC-SNS neuroimmune interface. However, age seems to alter this neuroimmune interface and thus could influence the neuroinvasion in prion pathogenesis.


Asunto(s)
Células Dendríticas Foliculares/patología , Ganglios Linfáticos Agregados/patología , Scrapie/patología , Bazo/patología , Sistema Nervioso Simpático/patología , Animales , Células Dendríticas Foliculares/metabolismo , Inyecciones Intraperitoneales , Ratones , Ratones Endogámicos C57BL , Microscopía Confocal , Proteína Proteolipídica de la Mielina/metabolismo , Fibras Nerviosas/metabolismo , Fibras Nerviosas/patología , Red Nerviosa/metabolismo , Red Nerviosa/patología , Proteínas PrPSc/metabolismo , Proteínas/metabolismo , Estadística como Asunto , Tirosina 3-Monooxigenasa/metabolismo
7.
Vet J ; 199(1): 115-22, 2014 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-24321368

RESUMEN

Articular cartilage defects are prevalent in metacarpo/metatarsophalangeal (MCP/MTP) joints of horses. The aim of this study was to determine and compare the sensitivity and specificity of 3-Tesla magnetic resonance imaging (3-T MRI) and computed tomography arthrography (CTA) to identify structural cartilage defects in the equine MCP/MTP joint. Forty distal cadaver limbs were imaged by CTA (after injection of contrast medium) and by 3-T MRI using specific sequences, namely, dual-echo in the steady-state (DESS), and sampling perfection with application-optimised contrast using different flip-angle evolutions (SPACE). Gross anatomy was used as the gold standard to evaluate sensitivity and specificity of both imaging techniques. CTA sensitivity and specificity were 0.82 and 0.96, respectively, and were significantly higher than those of MRI (0.41 and 0.93, respectively) in detecting overall cartilage defects (no defect vs. defect). The intra and inter-rater agreements were 0.96 and 0.92, respectively, and 0.82 and 0.88, respectively, for CT and MRI. The positive predictive value for MRI was low (0.57). CTA was considered a valuable tool for assessing cartilage defects in the MCP/MTP joint due to its short acquisition time, its specificity and sensitivity, and it was also more accurate than MRI. However, MRI permits assessment of soft tissues and subchondral bone and is a useful technique for joint evaluation, although clinicians should be aware of the limitations of this diagnostic technique, including reduced accuracy.


Asunto(s)
Artrografía/veterinaria , Cartílago/patología , Enfermedades de los Caballos/patología , Artropatías/veterinaria , Imagen por Resonancia Magnética/veterinaria , Tomografía Computarizada por Rayos X/veterinaria , Animales , Cadáver , Miembro Anterior , Miembro Posterior , Enfermedades de los Caballos/diagnóstico por imagen , Caballos , Artropatías/diagnóstico por imagen , Artropatías/patología
8.
J Neuroimmunol ; 262(1-2): 79-84, 2013 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-23932773

RESUMEN

The pharyngeal tonsil has recently been identified as a new participant in airborne contamination by the ovine scrapie agent. In the context of scrapie pathogenesis, we conducted a three-dimensional reconstruction of the innervation pattern in the lymphoid compartments of this tonsil. This model confirmed that very few nerve fibres penetrated the lymphoid follicles and suggested that the nerve fibre distribution in the interfollicular and subepithelial areas is more suitable with neuro-invasion through direct contact between these nerve fibres and prion-transporting cells prior to or after prion amplification in the germinal centre of the pharyngeal tonsil lymphoid follicles.


Asunto(s)
Tonsila Faríngea/inervación , Tomografía con Microscopio Electrónico/métodos , Tonsila Faríngea/patología , Tonsila Faríngea/ultraestructura , Animales , Proteína Ácida Fibrilar de la Glía/ultraestructura , Procesamiento de Imagen Asistido por Computador , Imagenología Tridimensional , Proteínas PrPSc/patogenicidad , Scrapie/patología , Ovinos , Oveja Doméstica
9.
Cell Tissue Res ; 348(1): 167-76, 2012 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-22427064

RESUMEN

Recent studies have established the involvement of nasal-associated lymphoid tissues, mainly the pharyngeal tonsil, in prion pathogenesis. However, the mechanisms of the associated neuroinvasion are still debated. To determine potential sites for prion neuroinvasion inside the ovine pharyngeal tonsil, the topography of heavy (200 kDa) and light (70 kDa) neurofilaments and of glial fibrillar acidic protein has been semi-quantitatively analysed inside the various compartments of the tonsil. The results show that the most innervated areas are the interfollicular area and the connective tissue located beneath the respiratory epithelium. The existence of rare synapses between follicular dendritic cells and nerve fibres inside the germinal centre indicates that this mechanism of neuroinvasion is possible but, since germinal centres of lymphoid follicles are poorly innervated, other routes of neuroinvasion are likely. The host PRNP genotype does not influence the pattern of innervation in these various tonsil compartments, unlike ageing during which an increase of nerve endings occurs in a zone of high trafficking cells beneath the respiratory epithelium. A minimal age-related increase of innervation inside the lymphoid follicles has also been observed. An increase in nerve fibre density around the lymphoid follicles, in an area rich in mobile cells such as macrophages and dendritic cells capable of capturing and conveying pathogen prion protein (PrPd), might ensure more efficient infectivity, not in the early phase but in the advanced phase of lymphoinvasion after the amplification of PrPd; alternatively, this area might even act as a direct site of entry during neuroinvasion.


Asunto(s)
Tonsila Faríngea/inmunología , Tonsila Faríngea/inervación , Sistema Nervioso/inmunología , Sistema Nervioso/patología , Priones/metabolismo , Oveja Doméstica/inmunología , Envejecimiento/patología , Animales , Crioultramicrotomía , Células Dendríticas Foliculares/inmunología , Células Dendríticas Foliculares/patología , Genotipo , Centro Germinal/metabolismo , Centro Germinal/patología , Proteína Ácida Fibrilar de la Glía/metabolismo , Microscopía Confocal , Fibras Nerviosas/metabolismo , Fibras Nerviosas/patología , Proteínas de Neurofilamentos/metabolismo , Scrapie/inmunología , Scrapie/patología
10.
Vet Immunol Immunopathol ; 141(1-2): 26-32, 2011 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-21353313

RESUMEN

Although the alimentary tract has been suggested as the most likely portal of entry in natural scrapie, a growing amount of data indicates that the respiratory system and more specifically the pharyngeal tonsils serve as a natural portal of entry for scrapie. This study describes for the first time the broad cell populations in the lymphoid compartment of pharyngeal tonsils and more specifically inside the lymphoid follicles where the scrapie agent accumulates during the period of latency. Follicular dendritic cells (FDCs), stromal cells located in the light zone of the germinal centre of lymphoid follicles, seem to be the principal causal factor in the accumulation of the infectious agent in transmissible spongiform encephalopathy (TSE) diseases. Knowing that efficient lymphoreticular prion propagation requires PrPc expression, we analysed the expression of PrPc with the mouse monoclonal antibody Pri 909 both in situ and on FDC-cluster-enriched cell suspensions. In situ, a positive staining was observed in the germinal centre of pharyngeal lymph follicles. The germinal centre labelling was due to the presence of a follicular dendritic network as revealed after immunogold staining of isolated FDC clusters. Our results suggest that the pharyngeal lymphoreticular system and more specifically PrPc expressing follicular dendritic cells could serve as a prion "reservoir" during the latency phase, thus playing a key role during the scrapie lymphoinvasion.


Asunto(s)
Tonsila Faríngea/patología , Células Dendríticas Foliculares/patología , Scrapie/patología , Tonsila Faríngea/inmunología , Animales , Anticuerpos/inmunología , Células Dendríticas Foliculares/inmunología , Inmunohistoquímica/veterinaria , Proteínas PrPC/inmunología , Scrapie/inmunología
11.
Virchows Arch ; 451(6): 1057-65, 2007 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-17823814

RESUMEN

In transmissible spongiform encephalopathies (TSEs), the infectious agent, called PrPsc, an abnormal isoform of the cellular prion protein, accumulates and replicates in lymphoid organs before affecting the nervous system. To clarify the cellular requirements for the neuroinvasion of the scrapie agent from the lymphoid organs to the central nervous system, we have studied, by confocal microscopy, the innervations within Peyer's patches, mesenteric lymph nodes and the spleen of mice in physiological conditions and after oral exposure to prion. Contacts between nerve fibres and PrPsc-associated cells, dendritic cells (DCs) and follicular dendritic cells (FDCs), were evaluated in preclinical prion-infected mice. Using a double immunolabelling strategy, we demonstrated the lack of innervation of PrPsc-accumulating cells (FDCs). Contacts between nerve fibers and PrPsc-propagating cells (DCs) were detected in T-cell zones and cell-trafficking areas. This supports, for the first time, the possible implication of dendritic cells in the prion neuroinvasion process.


Asunto(s)
Células Dendríticas Foliculares/patología , Tejido Linfoide/patología , Fibras Nerviosas/patología , Scrapie/patología , Animales , Células Dendríticas Foliculares/metabolismo , Modelos Animales de Enfermedad , Técnicas para Inmunoenzimas , Ganglios Linfáticos/inervación , Ganglios Linfáticos/metabolismo , Ganglios Linfáticos/patología , Tejido Linfoide/metabolismo , Ratones , Ratones Endogámicos C57BL , Microscopía Confocal , Fibras Nerviosas/metabolismo , Ganglios Linfáticos Agregados/inervación , Ganglios Linfáticos Agregados/metabolismo , Ganglios Linfáticos Agregados/patología , Proteínas PrPC/metabolismo , Scrapie/metabolismo , Bazo/inervación , Bazo/metabolismo , Bazo/patología
12.
Histochem Cell Biol ; 128(3): 243-51, 2007 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-17622551

RESUMEN

In transmitted prion diseases the immune system supports the replication and the propagation of the pathogenic agent (PrPSc). DCs, which are mobile cells present in large numbers within lymph organs, are suspected to carry prions through the lymphoid system and to transfer them towards the peripheral nervous system. In this study, C57Bl/6 mice were orally inoculated with PrPSc (scrapie strain 139A) and sacrificed at the preclinical stages of the disease. Immunolabelled cryosections of Peyer's patches were analysed by confocal microscopy. Membrane prion protein expression was studied by flow cytometry. In Peyer's patches (PP), dissected at day one and day 105 after oral exposure to scrapie, we observed an increased population of DCs localised in the follicular-associated epithelium. On day 105, PrPSc was found in the follicles inside the PP of prion-infected mice. A subset of Peyer's patches DCs, which did not express cellular prion protein on their surface in non-infected mice conditions, was prion-positive in scrapie conditions. Within Peyer's patches oral scrapie exposure thus induced modifications of the homeostasis of DCs at the preclinical stages of the disease. These results give new arguments in favour of the implication of DCs in prion diseases.


Asunto(s)
Homeostasis/efectos de los fármacos , Ganglios Linfáticos Agregados/metabolismo , Proteínas PrPSc/administración & dosificación , Scrapie/metabolismo , Administración Oral , Animales , Células Dendríticas/efectos de los fármacos , Células Dendríticas/metabolismo , Epitelio/efectos de los fármacos , Epitelio/metabolismo , Ratones , Ratones Endogámicos C57BL , Ganglios Linfáticos Agregados/efectos de los fármacos , Ganglios Linfáticos Agregados/patología , Proteínas PrPSc/biosíntesis , Proteínas PrPSc/metabolismo , Scrapie/patología
13.
Cell Tissue Res ; 329(1): 35-44, 2007 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-17406903

RESUMEN

During preclinical stages of cattle orally infected with bovine spongiform encephalopathy (BSE), the responsible agent is confined to ileal Peyer's patches (IPP), namely in nerve fibers and in lymph follicles, before reaching the peripheral and central nervous systems. No infectivity has been reported in other bovine lymphoid organs, including jejunal Peyer's patches (JPP). To determine the potential sites for prion neuroinvasion in IPP, we analyzed the mucosal innervation and the interface between nerve fibers and follicular dendritic cells (FDC), two dramatic influences on neuroinvasion. Bovine IPP were studied at three ages, viz., newborn calves, calves less than 12 months old, and bovines older than 24 months, and the parameters obtained were compared with those of JPP. No differences in innervation patterns between IPP and JPP were found. The major difference observed was that, in calves of less than 12 months, IPP were the major mucosal-associated lymphoid organ that possessed a large number of follicles with extended FDC networks. Using a panel of antibodies, we showed that PP in 24-month-old bovines were highly innervated at various strategic sites assumed to be involved in the invasion and replication of the BSE pathogen: the suprafollicular dome, T cell area, and germinal centers. In PP in calves of less than 12 months old, no nerve fibers positive for the neurofilament markers NF-L (70 kDa) and NF-H (200 kDa) were observed in contact with FDC. Thus, in view of the proportion of these protein subunits present in neurofilaments, the innervation of the germinal centers can be said to be an age-dependent dynamic process. This variation in innervation might influence the path of neuroinvasion and, thus, the susceptibility of bovines to the BSE agent.


Asunto(s)
Envejecimiento , Encefalopatía Espongiforme Bovina/transmisión , Íleon/inervación , Yeyuno/inervación , Ganglios Linfáticos Agregados/inervación , Priones , Envejecimiento/inmunología , Envejecimiento/metabolismo , Envejecimiento/patología , Animales , Bovinos , Células Dendríticas Foliculares/inmunología , Células Dendríticas Foliculares/metabolismo , Células Dendríticas Foliculares/patología , Encefalopatía Espongiforme Bovina/inmunología , Encefalopatía Espongiforme Bovina/patología , Centro Germinal/inmunología , Centro Germinal/metabolismo , Centro Germinal/patología , Íleon/inmunología , Íleon/metabolismo , Inmunohistoquímica , Yeyuno/inmunología , Yeyuno/metabolismo , Yeyuno/patología , Fibras Nerviosas/inmunología , Fibras Nerviosas/metabolismo , Fibras Nerviosas/patología , Proteínas de Neurofilamentos/biosíntesis , Proteínas de Neurofilamentos/inmunología , Ganglios Linfáticos Agregados/metabolismo , Ganglios Linfáticos Agregados/patología , Priones/inmunología , Priones/metabolismo
14.
Microsc Res Tech ; 66(1): 1-9, 2005 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-15816033

RESUMEN

In this study, we examined where immune cells and nerve fibres are located in mouse Peyer's patches, with a view to identifying potential sites for neuroinvasion by prions. Special attention was paid to dendritic cells, viewed as candidate transporters of infectious prion. Double immunofluorescence labellings with anti-CD11c antibody and marker for other immune cells (B cells, T cells, follicular dendritic cells) were carried out and analysed by confocal microscopy on Peyer's patch cryosections. To reveal the extensive ganglionated networks of the myenteric and submucosal plexi and the sparse meshworks of nerve strands, we used antibodies directed against different neurofilament subunits or against glial fibrillary acidic protein. In the suprafollicular dome, dendritic cells connect, via their cytoplasmic extensions, enterocytes with M cells of the follicle-associated epithelium. They are also close to B and T cells. Nerve fibres are detected in the suprafollicular dome, notably in contact with dendritic cells. Similar connections between dendritic cells, T cells, and nerve fibres are seen in the interfollicular region. Germinal centres are not innervated; inside them dendritic cells establish contacts with follicular dendritic cells and with B cells. After immunolabelling of normal prion protein, dendritic cells of the suprafollicular dome are intensely positive labelled.


Asunto(s)
Células Dendríticas/metabolismo , Fibras Nerviosas/metabolismo , Ganglios Linfáticos Agregados/metabolismo , Animales , Antígeno CD11c/metabolismo , Técnica del Anticuerpo Fluorescente , Secciones por Congelación , Ratones , Ratones Endogámicos C57BL , Proteínas PrPC/metabolismo
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