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1.
Nucleic Acids Res ; 39(20): 8952-9, 2011 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-21803791

RESUMEN

-1 Programmed ribosomal frameshifting (PRF) in synthesizing the gag-pro precursor polyprotein of Simian retrovirus type-1 (SRV-1) is stimulated by a classical H-type pseudoknot which forms an extended triple helix involving base-base and base-sugar interactions between loop and stem nucleotides. Recently, we showed that mutation of bases involved in triple helix formation affected frameshifting, again emphasizing the role of the triple helix in -1 PRF. Here, we investigated the efficiency of hairpins of similar base pair composition as the SRV-1 gag-pro pseudoknot. Although not capable of triple helix formation they proved worthy stimulators of frameshifting. Subsequent investigation of ∼30 different hairpin constructs revealed that next to thermodynamic stability, loop size and composition and stem irregularities can influence frameshifting. Interestingly, hairpins carrying the stable GAAA tetraloop were significantly less shifty than other hairpins, including those with a UUCG motif. The data are discussed in relation to natural shifty hairpins.


Asunto(s)
Sistema de Lectura Ribosómico , Proteínas de Fusión gag-pol/genética , ARN Mensajero/química , ARN Viral/química , Composición de Base , Disparidad de Par Base , Células HeLa , Humanos , Virus del Mono Mason-Pfizer/genética , Conformación de Ácido Nucleico
2.
Nucleic Acids Res ; 38(21): 7665-72, 2010 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-20639537

RESUMEN

Simian retrovirus type-1 uses programmed ribosomal frameshifting to control expression of the Gag-Pol polyprotein from overlapping gag and pol open-reading frames. The frameshifting signal consists of a heptanucleotide slippery sequence and a downstream-located 12-base pair pseudoknot. The solution structure of this pseudoknot, previously solved by NMR [Michiels,P.J., Versleijen,A.A., Verlaan,P.W., Pleij,C.W., Hilbers,C.W. and Heus,H.A. (2001) Solution structure of the pseudoknot of SRV-1 RNA, involved in ribosomal frameshifting. J. Mol. Biol., 310, 1109-1123] has a classical H-type fold and forms an extended triple helix by interactions between loop 2 and the minor groove of stem 1 involving base-base and base-sugar contacts. A mutational analysis was performed to test the functional importance of the triple helix for -1 frameshifting in vitro. Changing bases in L2 or base pairs in S1 involved in a base triple resulted in a 2- to 5-fold decrease in frameshifting efficiency. Alterations in the length of L2 had adverse effects on frameshifting. The in vitro effects were well reproduced in vivo, although the effect of enlarging L2 was more dramatic in vivo. The putative role of refolding kinetics of frameshifter pseudoknots is discussed. Overall, the data emphasize the role of the triple helix in -1 frameshifting.


Asunto(s)
Sistema de Lectura Ribosómico , Virus del Mono Mason-Pfizer/genética , ARN Viral/química , Secuencias Reguladoras de Ácido Ribonucleico , Regulación Viral de la Expresión Génica , Mutación , Conformación de Ácido Nucleico
3.
J Mol Biol ; 385(3): 733-47, 2009 Jan 23.
Artículo en Inglés | MEDLINE | ID: mdl-19059415

RESUMEN

Transcriptional polarity occurs in Escherichia coli when cryptic Rho-dependent transcription terminators become activated as a consequence of reduced translation. Increased spacing between RNA polymerase and the leading ribosome allows the transcription termination factor Rho to bind to mRNA, migrate to the RNA polymerase, and induce termination. Transcriptional polarity results in decreased synthesis of inefficiently translated mRNAs and, therefore, in decreased expression not only of downstream genes in the same operon (intercistronic polarity) but also of the cistron in which termination occurs (intracistronic polarity). To quantitatively measure the effect of different levels of translation on intracistronic transcription termination, the polarity-prone lacZ reporter gene was fused to a range of mutated ribosome binding sites, repressed to different degrees by local RNA structure. The results show that polarity gradually increases with decreasing frequency of translational initiation, as expected. Closer analysis, with the help of a newly developed kinetic model, reveals that efficient intracistronic termination requires very low translational initiation frequencies. This finding is unexpected because Rho is a relatively small protein that binds rapidly to its RNA target, but it appears to be true also for other examples of transcriptional polarity reported in the literature. The conclusion must be that polarity is more complex than just an increased exposure of the Rho binding site as the spacing between the polymerase and the leading ribosome becomes larger. Biological consequences and possible mechanisms are discussed.


Asunto(s)
ARN Mensajero/metabolismo , Transcripción Genética , Secuencia de Bases , Escherichia coli/genética , Genes Bacterianos , Cinética , Conformación de Ácido Nucleico , Biosíntesis de Proteínas , ARN Mensajero/química
4.
Mol Microbiol ; 69(5): 1278-89, 2008 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-19172759

RESUMEN

Transcriptional polarity in Escherichia coli occurs when cryptic Rho-dependent transcription terminators become activated as a consequence of reduced translation. Whether this is due to an increased spacing between the RNA polymerase and the leading ribosome or to prior functional inactivation of a subpopulation of the mRNAs has been a matter of discussion. Transcriptional polarity results in decreased synthesis of inefficiently translated mRNAs and therefore in decreased expression of downstream genes in the same operon (intercistronic polarity). By analogy, expression of the gene in which the conditional termination occurs is also expected to decrease, but this has so far not been demonstrated experimentally. To study the relevance of this intracistronic polarity for expression regulation in vivo, the polarity-prone IacZ reporter gene was fused to a range of mutated ribosome binding sites, repressed to different degrees by local RNA structure. Quantitative analysis of protein and mRNA synthesis shows that polarity occurs on functionally active mRNA molecules and that it indeed affects expression of the cistron carrying the terminator, thus enhancing the effect of translational repression. These findings point to a novel regulatory function of transcriptional polarity, reminiscent of transcriptional attenuation but opposite in effect.


Asunto(s)
Proteínas de Escherichia coli/metabolismo , Escherichia coli/genética , Biosíntesis de Proteínas , Transcripción Genética , Secuencia de Bases , Escherichia coli/química , Proteínas de Escherichia coli/química , Operón Lac , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , ARN Mensajero/química , ARN Mensajero/genética , Ribosomas/metabolismo
5.
J Virol ; 81(12): 6771-4, 2007 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-17428856

RESUMEN

A 190-nucleotide (nt) packaging signal (PS) located in the 3' end of open reading frame 1b in the mouse hepatitis virus, a group IIa coronavirus, was previously postulated to direct genome RNA packaging. Based on phylogenetic data and structure probing, we have identified a 95-nt hairpin within the 190-nt PS domain which is conserved in all group IIa coronaviruses but not in the severe acute respiratory syndrome coronavirus (group IIb), group I coronaviruses, or group III coronaviruses. The hairpin is composed of six copies of a repeating structural subunit that consists of 2-nt bulges and 5-bp stems. We propose that repeating AA bulges are characteristic features of group IIa PSs.


Asunto(s)
Coronavirus/genética , Genoma Viral , Secuencia de Bases , Bases de Datos de Proteínas , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Sistemas de Lectura Abierta , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Virus ARN/genética , Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo/genética , Análisis de Secuencia de ADN , Especificidad de la Especie
6.
J Bacteriol ; 189(9): 3581-90, 2007 May.
Artículo en Inglés | MEDLINE | ID: mdl-17337575

RESUMEN

The antibiotic kirromycin inhibits prokaryotic protein synthesis by immobilizing elongation factor Tu (EF-Tu) on the elongating ribosome. Streptomyces ramocissimus, the producer of kirromycin, contains three tuf genes. While tuf1 and tuf2 encode kirromycin-sensitive EF-Tu species, the function of tuf3 is unknown. Here we demonstrate that EF-Tu3, in contrast to EF-Tu1 and EF-Tu2, is resistant to three classes of EF-Tu-targeted antibiotics: kirromycin, pulvomycin, and GE2270A. A mixture of EF-Tu1 and EF-Tu3 was sensitive to kirromycin and resistant to GE2270A, in agreement with the described modes of action of these antibiotics. Transcription of tuf3 was observed during exponential growth and ceased upon entry into stationary phase and therefore did not correlate with the appearance of kirromycin in stationary phase; thus, it is unlikely that EF-Tu3 functions as a resistant alternative for EF-Tu1. EF-Tu3 from Streptomyces coelicolor A3(2) was also resistant to kirromycin and GE2270A, suggesting that multiple antibiotic resistance is an intrinsic feature of EF-Tu3 species. The GE2270A-resistant character of EF-Tu3 demonstrated that this divergent elongation factor is capable of substituting for EF-Tu1 in vivo.


Asunto(s)
Aminoglicósidos/farmacología , Antibacterianos/farmacología , Factor Tu de Elongación Peptídica/antagonistas & inhibidores , Péptidos Cíclicos/farmacología , Streptomyces/efectos de los fármacos , Farmacorresistencia Bacteriana , Regulación Bacteriana de la Expresión Génica , Modelos Moleculares , Factor Tu de Elongación Peptídica/genética , Factor Tu de Elongación Peptídica/metabolismo , Piridonas/metabolismo , Piridonas/farmacología , ARN Bacteriano/biosíntesis , ARN Mensajero/biosíntesis , Streptomyces/genética , Streptomyces/metabolismo , Streptomyces coelicolor/efectos de los fármacos , Tiazoles/farmacología , Transcripción Genética
7.
J Gen Virol ; 87(Pt 11): 3397-3407, 2006 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-17030876

RESUMEN

Structure prediction of the 5' non-translated region (NTR) of four iflavirus RNAs revealed two types of potential internal ribosome entry site (IRES), which are discriminated by size and level of complexity, in this group of viruses. In contrast to the intergenic IRES of dicistroviruses, the potential 5' IRES structures of iflaviruses do not have pseudoknots. To test the activity of one of these, a bicistronic construct was made in which the 5' NTR of Varroa destructor virus 1 (VDV-1) containing a putative IRES was cloned in between two reporter genes, enhanced green fluorescent protein and firefly luciferase (Fluc). The presence of the 5' NTR of VDV-1 greatly enhanced the expression levels of the second reporter gene (Fluc) in Lymantria dispar Ld652Y cells. The 5' NTR was active in a host-specific manner, as it showed lower activity in Spodoptera frugiperda Sf21 cells and no activity in Drosophila melanogaster S2 cells.


Asunto(s)
Regiones no Traducidas 5'/genética , Regulación de la Expresión Génica , Picornaviridae/genética , ARN Viral/genética , Regiones no Traducidas 5'/metabolismo , Algoritmos , Animales , Composición de Base , Secuencia de Bases , Northern Blotting , Células Cultivadas , Genes Reporteros/genética , Genes Virales/genética , Insectos , Luciferasas de Luciérnaga/genética , Luciferasas de Luciérnaga/metabolismo , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , ARN Mensajero/análisis , Secuencias Reguladoras de Ácidos Nucleicos , Ribosomas/metabolismo , Especificidad de la Especie , Transfección
8.
J Mol Biol ; 341(5): 1205-14, 2004 Aug 27.
Artículo en Inglés | MEDLINE | ID: mdl-15321716

RESUMEN

Empty capsids (artificial top component) of turnip yellow mosaic virus were co-crystallized with an encapsidation initiator RNA hairpin. No clear density was observed for the RNA, but there were clear differences in the conformation of a loop of the coat protein at the opening of the pentameric capsomer (formed by five A-subunits) protruding from the capsid, compared to the corresponding loop in the intact virus. Further differences were found at the N terminus of the A-subunit. These differences have implications for the mechanism of decapsidation of the virus, required for infection.


Asunto(s)
Cápside/química , Estructura Cuaternaria de Proteína , Tymovirus/química , Proteínas Virales/química , Cristalografía por Rayos X , Modelos Moleculares , Conformación de Ácido Nucleico , Estructura Secundaria de Proteína , Subunidades de Proteína/química , ARN Viral/química , Tymovirus/genética
9.
J Virol ; 78(8): 4003-10, 2004 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-15047816

RESUMEN

For various groups of plant viruses, the genomic RNAs end with a tRNA-like structure (TLS) instead of the 3' poly(A) tail of common mRNAs. The actual function of these TLSs has long been enigmatic. Recently, however, it became clear that for turnip yellow mosaic virus, a tymovirus, the valylated TLS(TYMV) of the single genomic RNA functions as a bait for host ribosomes and directs them to the internal initiation site of translation (with N-terminal valine) of the second open reading frame for the polyprotein. This discovery prompted us to investigate whether the much larger TLSs of a different genus of viruses have a comparable function in translation. Brome mosaic virus (BMV), a bromovirus, has a tripartite RNA genome with a subgenomic RNA4 for coat protein expression. All four RNAs carry a highly conserved and bulky 3' TLS(BMV) (about 200 nucleotides) with determinants for tyrosylation. We discovered TLS(BMV)-catalyzed self-tyrosylation of the tyrosyl-tRNA synthetase but could not clearly detect tyrosine incorporation into any virus-encoded protein. We established that BMV proteins do not need TLS(BMV) tyrosylation for their initiation. However, disruption of the TLSs strongly reduced the translation of genomic RNA1, RNA2, and less strongly, RNA3, whereas coat protein expression from RNA4 remained unaffected. This aberrant translation could be partially restored by providing the TLS(BMV) in trans. Intriguingly, a subdomain of the TLS(BMV) could even almost fully restore translation to the original pattern. We discuss here a model with a central and dominant role for the TLS(BMV) during the BMV infection cycle.


Asunto(s)
Bromovirus/genética , ARN Viral/química , ARN Viral/genética , Secuencia de Bases , Bromovirus/metabolismo , Prueba de Complementación Genética , Genoma Viral , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Biosíntesis de Proteínas , ARN de Transferencia/química , ARN de Transferencia/genética , Triticum/virología , Tirosina/química , Tirosina-ARNt Ligasa/química , Tirosina-ARNt Ligasa/genética , Tirosina-ARNt Ligasa/metabolismo , Proteínas Virales/química , Proteínas Virales/genética
10.
Proteins ; 55(2): 236-44, 2004 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-15048817

RESUMEN

RNA-coat protein interactions in turnip yellow mosaic virus (TYMV) have been shown to involve low pK proton-donating groups. Two different types of interaction have been proposed. In the so-called type I interaction, protonated C-residues interact with acidic amino acids at low pH, thereby providing a rationale for the high C-content (38%) of the genomic RNA. The type II interaction involves charged histidines interacting with phosphates of the RNA backbone. Site-directed mutagenesis of the TYMV coat protein and subsequent in vivo analysis were performed to distinguish between these two types of RNA-protein interaction. The results reveal a prominent role for the histidines H68 and H180, since mutation to an alanine residue inhibits symptom development on secondary leaves, indicating that spreading of the virus in the plant is blocked. Viral RNA and coat protein synthesis are not altered, showing that these two histidines may play a role in the process of RNA encapsidation. Overexpression of the TYMV coat protein in Escherichia coli leads to the formation of bona fide capsids, showing that the two histidines are not critical in capsid assembly. Mutagenesis of the acidic amino acids D11, E135, and D143 to alanine apparently did not interfere with virus viability. The functional role of the histidines during the infection cycle is discussed in terms of the structure of the coat protein, both at the level of amino acid sequence conservation among the members of the Tymoviridae family and as the three-dimensional structure of the coat protein.


Asunto(s)
Proteínas de la Cápside/química , Proteínas de la Cápside/metabolismo , Cápside/química , Histidina/metabolismo , ARN Viral/metabolismo , Tymovirus/química , Tymovirus/fisiología , Alanina/genética , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Sitios de Unión , Brassica/virología , Proteínas de la Cápside/genética , Secuencia Conservada , Escherichia coli , Histidina/genética , Modelos Moleculares , Datos de Secuencia Molecular , Enfermedades de las Plantas/virología , Hojas de la Planta/virología , Estructura Secundaria de Proteína , Tymovirus/genética , Ensamble de Virus/genética , Ensamble de Virus/fisiología
11.
Nucleic Acids Res ; 32(5): 1721-30, 2004.
Artículo en Inglés | MEDLINE | ID: mdl-15020708

RESUMEN

The subgenomic RNA 2 of tobacco necrosis virus A (TNV sgRNA2) encodes the viral coat protein, is unpolyadenylated and presumably uncapped. Here, we show that TNV sgRNA2 is translated cap independently. This cap-independent translation requires the leader and a 140 nt element of the trailer both in wheat germ extract and in tobacco protoplasts. Similar to barley yellow dwarf virus (BYDV), the TNV 5' and 3' elements stimulate translation synergistically. Computer-aided phylogenetic analysis of the secondary structure of the TNV trailer revealed that the 3' translation element is part of a major conserved stem-loop that contains similarities to structures in the BYDV 3' translation element. These data suggest that the translation mechanisms of TNV sgRNA2 and BYDV RNA are related. To further characterize this relationship, we tested whether cooperativity exists between TNV sgRNA2 and BYDV 5' and 3' elements. We found that the TNV sgRNA2 5' element stimulates translation synergistically with the BYDV 3' element in vitro. This finding is the first evidence for conservation of structures that enable a 5'-3' interaction stimulating cap-independent translation.


Asunto(s)
Regiones no Traducidas 3' , Regiones no Traducidas 5' , Luteovirus/genética , Biosíntesis de Proteínas , ARN Viral/química , Virus Satélite de la Necrosis del Tabaco/genética , Secuencia de Bases , Proteínas de la Cápside/genética , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Filogenia , Caperuzas de ARN
12.
Microsc Res Tech ; 65(4-5): 235-45, 2004 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-15630685

RESUMEN

Nowadays, the development of experimental procedures for the determination of the secondary structure of RNA molecules is taking advantage of the novel single-molecule probing and imaging techniques. We report a method for the mapping of the secondary structure of RNA molecules spread on a flat surface by means of the atomic force microscope. Globular domains comprising groups of RNA secondary and tertiary structure elements separated by unstructured domains can be discerned in the micrographs and their position along the molecule contour can be measured directly on unstained specimens. We have analyzed the morphology of a population of single molecules of 3' fragments of the Turnip Yellow Mosaic Virus RNA shorter than 1 kb in different temperature and electrolytic conditions. We found a satisfying agreement of the shape of the imaged structures with previously available evidence. The method we have developed can be used to map also different types of RNA molecules and has the advantage of showing the distribution of the single molecule conformations within the population.


Asunto(s)
Microscopía de Fuerza Atómica/métodos , Conformación de Ácido Nucleico , ARN Viral/química , Tymovirus/química , Procesamiento de Imagen Asistido por Computador , Nanotecnología , Tymovirus/genética
13.
Nucleic Acids Res ; 31(17): 5003-15, 2003 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-12930950

RESUMEN

The plant gene enod40 is highly conserved among legumes and also present in various non-legume species. It is presumed to play a central regulatory role in the Rhizobium-legume interaction, being expressed well before the initiation of cortical cell divisions resulting in nodule formation. Two small peptides encoded by enod40 mRNA as well as its secondary structure have been shown to be key elements in the signalling processes underlying nodule organogenesis. Here results concerning the secondary structure of mRNA of enod40 in soybean are presented. This study combined a theoretical approach, involving structure prediction and comparison, as well as structure probing. Our study indicates five conserved domains in enod40 mRNA among numerous leguminous species. Structure comparison suggests that some domains are also conserved in non-leguminous species and that an additional domain exists that was found only in leguminous species developing indeterminate nodules. Enzymatic and chemical probing data support the structure for three of the domains, and partially for the remaining two. The rest of the molecule appears to be less structured. Some of the domains include motifs, such as U-containing internal loops and bulges, which seem to be conserved. Therefore, they might be involved in the regulatory role of enod40 RNA.


Asunto(s)
Glycine max/genética , Conformación de Ácido Nucleico , Proteínas de Plantas/genética , ARN de Planta/química , Secuencia de Bases , Sitios de Unión/genética , Secuencia Conservada/genética , Modelos Moleculares , Datos de Secuencia Molecular , Filogenia , ARN de Planta/genética
14.
J Virol ; 77(13): 7452-8, 2003 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-12805444

RESUMEN

The RNA genome of turnip yellow mosaic virus (TYMV) consists of more than 6,000 nucleotides. During a study of the roles of the two hairpins located in its 90-nucleotide 5' untranslated region, it was observed that stabilization of the 5'-proximal hairpin leads to a delay in the development of symptoms on plants. This delay in symptom development for both locally and systemically infected leaves was found to be dependent on a change in the free energy of the hairpin caused by introduced mutations. A protoplast transfection assay revealed that the accumulation of plus-strand full-length RNA and subgenomic RNA, as well as protein expression levels, was affected by hairpin stability. Stabilization of this hairpin inhibited translation. A model is proposed in which a destabilized 5'-proximal hairpin allows maximal translation of the viral proteins. It is suggested that this hairpin may exist in close proximity to the 5' cap as long as its stability is low enough to enable translation. However, at an acidic pH, the hairpin structure becomes more stable and is functionally transformed into the initiation signal for viral packaging. Slightly acidic conditions can be found in chloroplasts, where TYMV assembly is driven by a low pH generated by active photosynthesis.


Asunto(s)
Cápside , Conformación de Ácido Nucleico , Biosíntesis de Proteínas , Tymovirus/genética , Secuencia de Bases , Datos de Secuencia Molecular , Transfección
15.
Cell ; 112(1): 123-9, 2003 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-12526799

RESUMEN

Turnip yellow mosaic virus (TYMV) has a genomic plus-strand RNA with a 5' cap followed by overlapping and different reading frames for the movement protein and polyprotein, while the distal coat protein cistron is translated from a subgenomic RNA. The 3'-untranslated region harbors a tRNA-like structure (TLS) to which a valine moiety can be added and it is indispensable for virus viability. Here, we report about a surprising interaction between TYMV-RNA-programmed ribosomes and 3'-valylated TLS that yields polyprotein with the valine N terminally incorporated by a translation mechanism resistant to regular initiation inhibitors. Disruption of the TLS exclusively abolishes polyprotein synthesis, which can be restored by adding excess TLS in trans. Our observations imply a novel eukaryotic mechanism for internal initiation of mRNA translation.


Asunto(s)
Imitación Molecular , Virus del Mosaico/genética , Biosíntesis de Proteínas , ARN de Transferencia/genética , ARN Viral , Ribosomas/metabolismo , Regiones no Traducidas 3'/química , Regiones no Traducidas 3'/genética , Regiones no Traducidas 3'/metabolismo , Modelos Genéticos , Virus del Mosaico/metabolismo , Estructura Secundaria de Proteína , ARN Mensajero/metabolismo , Ribosomas/efectos de los fármacos , Especificidad por Sustrato , Triticum , Tymovirus/genética , Valina/química , Valina/metabolismo , Proteínas Virales/biosíntesis , Proteínas Virales/química , Proteínas Virales/genética
16.
Biochimie ; 84(9): 913-23, 2002 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-12458084

RESUMEN

Many biologically active RNAs show a switch in their secondary structure, which is accompanied by changes in their function. Such changes in secondary structure often require trans-acting factors, e.g. RNA chaperones. However, several biologically active RNAs do not require trans-acting factors for this structural switch, which is therefore indicated here as a "self-induced switch". These self-induced structural switches have several characteristics in common. They all start from a metastable structure, which is maintained for some time allowing or blocking a particular function of the RNA. Hereafter, a structural element becomes available, e.g. during transcription, triggering a rapid transition into a stable conformation, which again is accompanied by either a gain or loss of function. A further common element of this type of switches is the involvement of a branch migration or strand displacement reaction, which lowers the energy barrier of the reaction sufficiently to allow rapid refolding. Here, we review a number of these self-induced switches in RNA secondary structure as proposed for several systems. A general model for this type of switches is presented, showing its importance in the biology of functionally active RNAs.


Asunto(s)
Conformación de Ácido Nucleico , ARN/química , ARN/genética , Animales , Secuencia de Bases , Genoma Bacteriano , Intrones , Modelos Genéticos , Datos de Secuencia Molecular , ARN Catalítico/química , ARN Catalítico/genética , Relación Estructura-Actividad , Termodinámica , Transcripción Genética
17.
Nucleic Acids Res ; 30(19): 4232-40, 2002 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-12364602

RESUMEN

Valine-accepting tRNA-like structures (TLSs) are found at the 3' ends of the genomic RNAs of most plant viruses belonging to the genera Tymovirus, Furovirus, Pomovirus and Pecluvirus, and of one Tobamovirus species. Sequence alignment of these TLSs suggests the existence of a tertiary D-loop-T-loop interaction consisting of 2 bp, analogous to those in the elbow region of canonical tRNAs. The conserved G(18).Psi(55) pair of regular tRNAs is found to covary in these TLSs between G.U (possibly also modified to G.Psi) and A.G. We have mutated the relevant bases in turnip yellow mosaic virus (TYMV) and examined the mutants for symptom development on Chinese cabbage plants and for accumulation of genetic reversions. Development of symptoms is shown to rely on the presence of either A.G or G.U in the original mutants or in revertants. This finding supports the existence and functional importance of this tertiary interaction. The fact that only G.U and A.G are accepted at this position appears to result from steric and energetic limitations related to the highly compact nature of the elbow region. We discuss the implications of these findings for the various possible functions of the valine-accepting TLS.


Asunto(s)
Conformación de Ácido Nucleico , Aminoacil-ARN de Transferencia/química , ARN Viral/química , Tymovirus/genética , Secuencia de Bases , Datos de Secuencia Molecular , Mutación , Plantas/virología , Aminoacil-ARN de Transferencia/genética , ARN Viral/genética , Homología de Secuencia de Ácido Nucleico , Relación Estructura-Actividad
18.
Proc Natl Acad Sci U S A ; 99(21): 13465-70, 2002 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-12361978

RESUMEN

The 5' UTR of turnip yellow mosaic virus RNA contains two conserved hairpins with internal loops consisting of C.C and C.A mismatches. In this article, evidence is presented indicating that the 5' proximal hairpin functions as an encapsidation initiation signal. Extensive mutagenesis studies on this hairpin and sequencing of virus progeny showed a clear preference for C.C and C.A mismatches within the internal loop. The importance of these mismatches lies in their pH-dependent protonation and stable base pair formation. Encapsidation efficiency was found to be severely affected for several mutants lacking the protonatable mismatches in the internal loop of the 5' proximal hairpin. Furthermore, gel mobility-shift assays were performed with various RNA hairpins and empty capsids with a hole. Protonatable hairpins containing C.C and/or C.A pairs were found to bind specifically to the interior of the protein shell under acidic conditions (pH 4.5) in the presence of spermidine. Based on these results we propose that this binding of protonated cytosines to the coat protein of turnip yellow mosaic virus may represent a new motif in RNA-protein interactions.


Asunto(s)
ARN Viral/química , Tymovirus/química , Regiones no Traducidas 5' , Disparidad de Par Base , Emparejamiento Base , Secuencia de Bases , Sitios de Unión , Cápside/química , Mutagénesis , Conformación de Ácido Nucleico , Protones , ARN Viral/genética , Tymovirus/genética , Tymovirus/fisiología , Ensamble de Virus
19.
J Gen Virol ; 83(Pt 8): 1875-1885, 2002 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-12124451

RESUMEN

Montana Myotis leukoencephalitis virus (MMLV), a virus isolated from bats, causes an encephalitis in small rodents reminiscent of flavivirus encephalitis in humans. The complete MMLV genome is 10690 nucleotides long and encodes a putative polyprotein of 3374 amino acids. The virus contains the same conserved motifs in genes that are believed to be interesting antiviral targets (NTPase/helicase, serine protease and RNA-dependent RNA polymerase) as flaviviruses of clinical importance. Phylogenetic analysis of the entire coding region has confirmed the classification of MMLV in the clade of the flaviviruses with no known vector (NKV) and within this clade to the Rio Bravo branch (both viruses have the bat as their vertebrate host). We have provided for the first time a comparative analysis of the RNA folding of the 3' UTR of the NKV flaviviruses (Modoc, Rio Bravo and Apoi viruses, in addition to MMLV). Structural elements in the 3' UTR that are preserved among other flaviviruses have been revealed, as well as elements that distinguish the NKV from the mosquito- and tick-borne flaviviruses. In particular, the pentanucleotide sequence 5' CACAG 3', which is conserved in all mosquito- and tick-borne flaviviruses, is replaced by the sequence 5' C(C/U)(C/U)AG 3' in the loop of the 3' long stable hairpin structure of all four NKV flaviviruses. The availability of this latter sequence motif allows us to designate a virus as either an NKV or a vector-borne flavivirus.


Asunto(s)
Regiones no Traducidas 3'/genética , Encefalitis Viral/virología , Flavivirus/clasificación , Flavivirus/genética , Genoma Viral , Filogenia , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Secuencia Conservada , Vectores de Enfermedades , Flavivirus/química , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , ARN Viral/genética , Análisis de Secuencia de ADN
20.
RNA ; 8(2): 229-36, 2002 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-11924567

RESUMEN

The translational enhancer domain (TED) of satellite tobacco necrosis virus (STNV) RNA stimulates translation of uncapped RNAs autonomously. Here we set out to identify the 5' and 3' extremities of TED and features of these sequences with respect to translation. We found that both in wheat germ extract and in tobacco protoplasts, the 5' border is confined to 3 nt. Mutational analysis revealed that the autonomous function of TED is sensitive to 5' flanking sequences. At the 3' end of TED, 23 nt have a cumulative, quantitative effect on translation in wheat germ extract, whereas in tobacco protoplasts, the most 3' 14 nt of these 23 nt do not enhance translation. The 5' and 3' sequence requirements triggered the development of a new secondary structure model. In this model, TED folds into a phylogenetically conserved stem-loop structure in which the essential 5' nucleotides base-pair with the 3' nucleotides that stimulate translation both in vitro and in vivo. Importantly, the 14 3' nucleotides in TED that stimulate translation in the wheat germ extract only do not require the predicted base-pairing in order to function. The discrepancy between in vitro and in vivo sequence requirements thus correlates with potential base-pairing requirements, opening the possibility that TED contains two functional domains.


Asunto(s)
Elementos de Facilitación Genéticos , Biosíntesis de Proteínas , Satélite de ARN/genética , ARN Viral/genética , Tombusviridae/genética , Secuencia de Bases , Cartilla de ADN , Cinética , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Plásmidos , Protoplastos/virología , Nicotiana/virología , Transcripción Genética , Triticum/genética , Triticum/virología
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