Correction: Podlutskii et al. Arabidopsis thaliana Accessions from the Chernobyl Exclusion Zone Show Decreased Sensitivity to Additional Acute Irradiation. Plants 2022, 11, 3142.

In the original publication [...].

Growth, antioxidant system, and phytohormonal status of barley cultivars contrasting in cadmium tolerance.

Cadmium leads to disturbance of plant growth, and the manifestation of toxicity can vary greatly in different genotypes within one species. In this work we studied the effect of Cd on growth, antioxidant enzyme activity, and phytohormonal status of four barley cultivars (cvs. Simfoniya, Mestnyj, Ca 220702, Malva). According to the earlier study on seedlings, these cultivars were contrast in tolerance to Cd: Simfoniya and Mestnyj are Cd-tolerant and Ca 220702 and Malva are Cd-sensitive. The results presented showed that barley plants accumulated more Cd in straw than in grain. Tolerant cultivars accumulated significantly less Cd in grain than sensitive ones. The leaf area appeared to be a growth parameter susceptible to Cd treatment. The significant differences in leaf area values depended on Cd contamination and were not associated with cultivars' tolerance. Tolerance of cultivars was contingent on the activity of the antioxidant defense system. Indeed, activity of enzymes decreased in sensitive cultivars Ca 220702 and Malva under Cd stress. In contrast, in tolerant cultivars, increased activity of guaiacol peroxidase was revealed. The concentrations of abscisic acid and salicylic acid mostly increased as a result of Cd treatment, while the concentrations of auxins and trans-zeatin either decreased or did not change. The results obtained indicate that antioxidant enzymes and phytohormones play an important role in the response of barley plants to elevated concentrations of cadmium; however, these parameters are not able to explain the differentiation of barley cultivars in terms of tolerance to cadmium at the seedling stage. Therefore, barley intraspecific polymorphism for cadmium resistance is determined by the interplay of antioxidant enzymes, phytohormones, and other factors that require further elucidation.

Arabidopsis thaliana Accessions from the Chernobyl Exclusion Zone Show Decreased Sensitivity to Additional Acute Irradiation.

Chronic ionising radiation exposure is a main consequence of radioactive pollution of the environment. The development of functional genomics approaches coupled with morphological and physiological studies allows new insights into plant adaptation to life under chronic irradiation. Using morphological, reproductive, physiological, and transcriptomic experiments, we evaluated the way in which Arabidopsis thaliana natural accessions from the Chernobyl exclusion zone recover from chronic low-dose and acute high-dose γ-irradiation of seeds. Plants from radioactively contaminated areas were characterized by lower germination efficiency, suppressed growth, decreased chlorophyll fluorescence, and phytohormonal changes. The transcriptomes of plants chronically exposed to low-dose radiation indicated the repression of mobile genetic elements and deregulation of genes related to abiotic stress tolerance. Furthermore, these chronically irradiated natural accessions showed higher tolerance to acute 150 Gy γ-irradiation of seeds, according to transcriptome and phytohormonal profiles. Overall, the lower sensitivity of the accessions from radioactively contaminated areas to acute high-dose irradiation may come at the cost of their growth performance under normal conditions.

Seed Gamma Irradiation of Arabidopsis thaliana ABA-Mutant Lines Alters Germination and Does Not Inhibit the Photosynthetic Efficiency of Juvenile Plants.

Plant growth response to γ-irradiation includes stimulating or inhibitory effects depending on plant species, dose applied, stage of ontogeny and other factors. Previous studies showed that responses to irradiation could depend on ABA accumulation and signaling. To elucidate the role of ABA in growth and photosynthetic responses to irradiation, lines Col-8, abi3-8 and aba3 -1 of Arabidopsis thaliana were used. Seeds were γ-irradiated using 60Co in the dose range 50-150 Gy. It was revealed that the dose of 150 Gy affected germination parameters of aba3 -1 and Col-8 lines, while abi3-8 line was the most resistant to the studied doses and even showed faster germination at early hours after γ-irradiation at 50 Gy. These results suggest that susceptibility to ABA is probably more important for growth response to γ-irradiation than ABA synthesis. The photosynthetic functioning of 16-day-old plants mainly was not disturbed by γ-irradiation of seeds, and no indication of photosystem II photoinhibition was noticed, revealing the robustness of the photosynthetic system of A. thaliana. Glutathione peroxidase activity and ABA concentrations in plant tissues were not affected in the studied dose range. These results contribute to the understanding of germination and photosynthesis fine-tuning and of mechanisms of plant tolerance to ionizing radiation.