RESUMEN
The objective of the present study was to select and develop simpler methods for the quantitative determination of baclofen in blood with the use of HPLC and tandem MS (MS-MS) techniques and its qualitative determination in cadaveric organs by the GC/MS technique. These mathods were shown to be suitable for the purpose of forensic medical analysis, clinical, toxicological, and therapeutic monitoring. The special emphasis is laid on the methods used to investigate the biological materials obtained from the subjects who died from baclofen intoxication.
Asunto(s)
Baclofeno/análisis , Toxicología Forense/métodos , Relajantes Musculares Centrales/análisis , Suicidio , Adulto , Baclofeno/sangre , Baclofeno/farmacocinética , Baclofeno/envenenamiento , Cromatografía Líquida de Alta Presión , Resultado Fatal , Femenino , Toxicología Forense/instrumentación , Humanos , Límite de Detección , Hígado/metabolismo , Relajantes Musculares Centrales/sangre , Relajantes Musculares Centrales/farmacocinética , Relajantes Musculares Centrales/envenenamiento , Intoxicación/sangre , Intoxicación/etiología , Intoxicación/metabolismo , Reproducibilidad de los Resultados , Espectrometría de Masas en Tándem , Distribución TisularRESUMEN
Integral characteristics of animal development (weight increase, maturing of biological systems) used to date are not adequate to the requirements of detailed biological evaluation of dietary fats. The linoleic acid content in the dietary fat and the eicosatrienoic acid - eicosatetraenoic acid ratio (20:3/20:4 = HOLMAN coefficient) in the organism alone are also inadequate criteria. For this reason, the author attempted to establish metabolism-relevant law-like relationships between the fatty acid pattern of dietary fats and tissue lipids.
