Concentrations of Gaseous Transmitters during Catecholamine Damage to the Myocardium in Rats.

Gaseous transmitters were assayed in rat blood during catecholamine-induced damage to the heart. Hypercatecholaminemia was modeled by single subcutaneous injection of 0.1% epinephrine hydrochloride in a dose of 2 mg/kg. The blood concentrations of NO, H2S, and CO were measured. The catecholamine-induced damage to the myocardium resulted in phasic changes in the blood levels of gaseous transmitters: CO concentration increased in 1 h, H2S increased in 24 h, and NO concentration increased in 72 h after injection.

[Features of changes in levels of humoral factors regulating the system of hemostasis, in the late stages of pregnancy.]

The influence of humoral factors on the state of hemostasis in nonpregnant and pregnant women. There were 70 women aged from 22 to 32 years, of which the 1st group consisted of 50 patients with physiological pregnancy at term gestation 38-40 weeks, the 2nd group - 20 healthy non-pregnant women. All women was measured in the plasma levels of e-NOS, cGMP, PF4, ß-TG, adrenaline and noradrenaline by ELISA, in serum NOx, indicators of gemostaziogramma. The results showed a significant decrease in the blood e-NOS, NOx, cGMP, PF4, as well as increased levels of adrenaline and noradrenaline in the blood of pregnant women in 38-40 weeks of gestation in comparison with nonpregnant women.

[Comparative characteristics of the hemostatic potential in cord blood and in blood from premature and full-term newborns after birth according to the thromboelastography.]

The comparative characteristic of indicators of thromboelastography of umbilical cord blood and venous blood for 1-3 days of life at full-term and premature newborns is carried out. The study included 40 full term and 68 preterm infants at 1-5 days of life. Body weight of children was 3413.8±54.6 g and 2039.4±75.9 g, gestational age - 40.0±0.3 and 33.5±0.33 weeks, respectively. Mixed umbilical cord blood and venous blood taken from the ulnar vein of a child for 1-3 days of life were studied. The obtained data indicate that the main focus in the performance of thromboelastograms in premature and full-term newborns in the umbilical and venous blood are preserved. In preterm infants in cord blood decreased activity of the plasma level and functional activity of platelets, as well as reduced coagulation potential in General. After birth, premature newborns in the venous blood showed a slowdown in blood clotting, a decrease in the rate of formation and strength of clots. There is also a decrease in the functional activity of platelets, coagulation index and a more pronounced change in hemostatic potential in the direction of hypocoagulation. In full-term children, changes in the functional state of platelets and clot lysis in the umbilical and venous blood after birth do not change significantly. Hypercoagulable orientation of hemostasis in these children at birth was established.

[The evaluation of oxidation stress in umbilical blood and lysate of endothelial cells of vessels of umbilical cord of newborns.]

The sampling of newborns (n=28) was examined. Out of them, 18 premature children formed main group (gestation age from 28 to35 weeks, body mass 2067,3±76,7g) and 10 mature children formed comparison group (gestation age from 38 to 41 weeks, body mass 3380,2±57,2g) born from mothers with physiologically occurring pregnancy without extra-genital pathology. In all children, the detection of endothelial cells isolated from umbilical cord vein in mixed umbilical blood and lysate was applied and indices of free radical oxidation of lipids, characterizing oxidizing stress as well and anti-oxidation activity using chemiluminescence technique. The study results demonstrated in premature newborns increasing in umbilical blood of indices of fast flash chemiluminescence (Imax), light sun value (S) and tangent of kinetic curve slope (tga) and also increasing of parameters Imax and tga in lysate of endothelial cells of umbilical cord. Al these occurrences testify in these children development oxidation stress being accompanied by compensation increasing of antioxidant activity.

[The characteristics of function of endothelium in premature neonates with respiratory distress-syndrome].

The examined sampling consisted of 637 premature neonates in early neonatal period at 1-3 and 5-8 days of life. The analysis was applied to indices characterizing epithelium condition and regulating its function. It is noted that in premature neonates with respiratory distress-syndrome of derangement of regulation of function of endothelium are accompanied by increasing of hemostatic disorders and is characterized by increasing of thrombogenic and adhesive characteristics, decreasing of levels of VEGF, higher content of nitric oxide in the form of nitrites, cytokinemia and activation of complement systems.

[The markers of inflammation reaction and dysfunction of endothelium in pregnant women with hypertension disorders of various genesis].

The markers of endothelium dysfunction and factors of inflammation in pregnant women with hypertension with hypertension disorders of various genesis were analyzed. The clinical laboratory study was carried out on the basis of sampling of 158 women at pregnancy period of22-3 7 weeks. Out of this sampling 30 women had previously present chronic arterial hypertension, 3 0 women had chronic arterial hypertension and consecutive preeclampsia, 43 women had preeclampsia and 55 women had uncomplicated course of pregnancy without hypertension disorders (control group). It is established that in pregnant women with hypertension disorders of various genesis endothelial dysfunction and inflammation are developed/ This occurrence is confirmed by increasing of in blood of number of circulating desquamated endotheliocytes, C-reactive protein and homocystein in all groups; by increasing of serum level of t-PA, endothelin (1-21), MMP-2, sVCAM-1 and IL-6 under preeclampsia, including one consecutive to chronic arterial hypertension; by increasing of content of IL-6 in blood serum under chronic arterial hypertension with consecutive preeclampsia. The criteria are developed concerning serum content of t-PA, sVCAM-1, endothelin (1-21) and MMP-2 permitting to diagnose differentially previously present hypertension and preeclampsia, including consecutive one to chronic arterial hypertension.

[The evaluation of secretory immunity under herpetic affection of oral cavity].

The study is based upon the results of clinical laboratory examination of 33 patients with diagnosis of recurrent herpetic stomatitis. The control group included 26 healthy persons. In patients with manifestations of herpetic infection of mucous tunic of oral cavity the pronounced abnormalities of indicators of secretory immunity (lactoferrin, secretory immunoglobulin A) correlating with hygiene index are established. At the same time, indicator of antiviral immunity (alpha-interferon) characterized by absence of significant changes in this group of patients.

[The functional characteristics of hemostasis of full-term and premature newborn according results of thromboflexography].

The study was carried out using the sample of 40 full-term and 138 premature newborns at 1 - 15th day of live and 20 adult donors. The thromboflexograph TEG 5000 was applied. The study used citrate stabilized venous blood. In full-term newborns the activation of thrombin and fibrin formation (decreasing of constant of coagulation, increasing of velocity of fibrin formation) was detected as compared with adults. In premature newborns shortage of time period of development of reactions of enzyme cascade, shortage of time period of achievement of ultimate amplitude of forming clot and increasing of fibrinolysis were detected as compared with adult donors. The differences in hemostatic system of full-term and premature newborns are characterized by lower functional activity of thrombocytes, decreased strength of forming clots and lower blood coagulation index of premature newborns.

Effect of antioxidants and nitric oxide on activity of peritoneal macrophages in albino rats during normal pregnancy.

We compared activity of peritoneal macrophages in rats during normal pregnancy and under the action of NO-synthase inhibitor L-NAME, of E. coli LPS, SOD, and α-tocopherol analogue trolox. In non-pregnant rats, E. coli LPS stimulated peritoneal macrophages, while L-NAME produced a dose-dependent effect. During pregnancy, E. coli LPS activated phagocytosis, while antioxidants trolox, SOD, and L-NAME produced an inhibitory effect.

[The content of Clara's cells protein in highly premature newborns with respiration disorders].

The study was carried out to analyze the content of Clara's cells protein in highly premature newborns with respiration disorders in neonatal period. The sample of 106 premature newborns of gestational age 26-32 weeks and body mass at birth 660-1500 g was examined. The concentration of Clara's cells protein in bronchoalveolar liquid and blood serum was higher in patients with inborn pneumonia as compared with children with respiratory distress syndrome. The low content Clara's cells protein in highly premature newborns with pneumonia was related with subsequent development of bronchopulmonary dysplasia.

[Effect of alpha-tocopherol on activity of free-radical processes and erythron in a rat fetus with chronic nitrite intoxication].

The condition of erythron and the activity of free-radical processes in fetuses of rats with chronic nitrite intoxication were studied and the effects of alpha-tocopherol injection (150 mg/kg, i.m.) were evaluated. A long-term introduction of nitrites (0.2% solution of sodium nitrite, p.o.) led to an increase in the erythrocyte count, hemoglobin content, and free radical concentration and a decrease in the antioxidant potential. The injection of alpha-tocopherol on the background of nitrite intoxication made these negative changes less expressed than in the case of separate administration of sodium nitrite. The amount of free radicals in the blood serum of fetuses of rats treated with alpha-tocopherol decreased, while the activity of the antioxidant system increased.

[Some biochemical parameters in the placenta in discoordinated and powerless labors].

A number of biochemical parameters (total nitrites and nitrates (NO(x)), cyclic guanosine monophosphate (cGMP), nitrotyrosine, medium-weight molecules (MCM) in the placenta were determined in women with gestosis during discoordinated and powerless labor. Thirty placentas (10 placentas from parturients after discoordinated labor, 10 from those after powerless labor, 10 placentas as a control group) were examined. Changes in the parameters under study were found to result in the development of nitroxide and oxidant stresses and endotoxicosis. The biochemical parameters should be considered as placental criteria for the differential diagnosis of labor anomalies in gestosis, such as powerless and discoordinated labors.