1.
Anal Biochem
; 381(1): 175-7, 2008 Oct 01.
Artículo
en Inglés
| MEDLINE
| ID: mdl-18602361
RESUMEN
Multiple recombinant proteins can be expressed simultaneously by inoculating multiple seed cultures into a single growth medium and inducing protein expression at a single time point. Up to three recombinant proteins can be individually purified from such a mixed culture (cocultivation) through the use of a combination of a multihistidine and a modified intein as affinity tags and the Ni sepharose and chitin as affinity matrices. This method may facilitate the study of protein complexes by rapidly obtaining multiple protein components in a single process and may potentially increase the efficiency of recombinant protein production at research and industrial scales.