Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed.

Natural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium, MTT, in cell culture of fibroblasts L929, human keratinocytes HaCat, and human endothelium EA.hy926 cell lines. The triplicate of the cosmetic formulation containing the extract was obtained regarding stability according to the procedures of the Brazilian Health Regulatory Agency (Anvisa). The irritability tests were performed using alternative methods, in vitro, chorioallantoic membrane assay (HET-CAM and CAM-TBS), and hemolysis test (RBC). The crude extract was not cytotoxic, IC50 index >780 mg/mL. The preservative system was effective against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, and Aspergillus niger. The emulsion was classified as non-irritable. The crude extract of the by-product of sapucaia seeds can be incorporated into a natural emulsion, safe for topical use.

Polyphenols, Antioxidants, and Wound Healing of Lecythis pisonis Seed Coats.

To better use the Lecythis pisonis Cambess. biomass, this study investigates whether Sapucaia seed coats present wound healing properties. We analyzed the antibacterial, antioxidant, and wound healing-promoting potentials, plus cytotoxicity and stimulation of vascular endothelial growth factor-A. The chemical composition was analyzed by positive ion mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. A total of 19 compounds were identified, such as proanthocyanidin A1, procyanidins A1, B2, and C1, epigallocatechin, and kaempferol (p-coumaroyl) glycoside. Potent antioxidant strength/index was verified for 2,2-diphenyl-1-picrylhydrazyl radical (IC50 = 0.99 µg/mL) and ferric reducing antioxidant power (IC50 = 1.09 µg/mL). The extract did not present cytotoxicity and promoted significant cell migration and/or proliferation of fibroblasts (p < 0.05). Vascular endothelial growth factor-A was stimulated dose-dependently at 6 µg/mL (167.13 ± 8.30 pg/mL), 12.5 µg/mL (210.3 ± 14.2 pg/mL), and 25 µg/mL (411.6 ± 29.4 pg/mL). Platelet-derived growth factor (PDGF) (0.002 µg/mL) was stimulated at 215.98 pg/mL. Staphylococcus aureus was susceptible to the extract, with a minimum inhibitory concentration of 31.25 µg/mL. The identified compounds benefit the antioxidant activity, promoting hemostasis for the wound healing process, indicating that this extract has the potential for use in dermatological cosmetics.

Estratégias transdisciplinares: o ato médico na compreensão de graduandos da área de saúde / Transdisciplinaries strategies: the "medical act" in the health undergraduation students' comprehension

Trata-se de uma pesquisa descritiva e exploratória, com abordagem qualitativa, voltada para analisar as repercussões do Ato Médico (AM) a partir do ponto de vista de estudantes de graduação da área de saúde e estimular o desenvolvimento do senso crítico acerca da transdisciplinaridade para harmonia do trabalho em equipe. Os sujeitos do estudo são 105 estudantes de graduação, a saber: 34 de Enfermagem (6º Período), 45 de Nutrição (2º Período), 17 de Fisioterapia (1º Período) e 9 de Medicina (4º Período) e o instrumento utilizado para coleta de dados limitado a uma questão aberta, visando captar os significados do ato médico para os mesmos. A coleta dos dados ocorreu no primeiro e segundo semestres de 2004. Os resultados apontam o AM como retrocesso para a transdisciplinaridade, em decorrência da indevida apropriação do conhecimento em saúde pela medicina, contrapondo a legislação vigente. Conclui-se que o incentivo às discussões sobre o tema com estudantes da área de saúde possibilita maior esclarecimento de cada categoria acerca do domínio de conhecimento das demais, sobretudo, na compreensão de que o AM infringe um dos mais importantes princípios da transdisciplinaridade, o trabalho em equipe.

It is a descriptive and exploratory research, with qualitative approach, gone back to analyze the repercussions of the “Medical Act” (MA) starting from the health sciences undergraduation students' point of view and to stimulate the development of the critical sense concerning the transdisciplinarity for harmony of the work in team. The subject of the study are 105 undergraduation students, 34 of Nursing (6th Semester), 45 of Nutrition (2nd Semester), 17 of Physiotherapy (1st Semester) and 9 of Medicine (4th Semester), and the instrument used for collection of data limited to one open subject, seeking to capture the meanings of the medical act for the same ones. The data collection happened in the first and second semesters of 2004. The results aim MA as setback for the transdisciplinarity, due to the improper appropriation of the knowledge in health for the medicine, opposing the effective legislation. It is ended that the incentive to the discussions on the theme with students of the area of health facilitates larger elucidate of each category concerning the domain of knowledge of the others, specially, in the understanding that AM infringes one of the most important beginnings of the transdisciplinarty, the work in team