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1.
Insects ; 10(9)2019 Aug 22.
Artículo en Inglés | MEDLINE | ID: mdl-31443396

RESUMEN

The lenses in compound eyes of butterflies and moths contain an array of nipple-shaped protuberances, or corneal nipples. Previous work has suggested that these nipples increase light transmittance and reduce the eye glare of moths that are inactive during the day. This work builds on but goes further than earlier analyses suggesting a functional role for these structures including, for the first time, an explanation of why moths are attracted to UV light. Using a phylogenetic approach and 3D optical modelling, we show empirically that these arrays have been independently lost from different groups of moths and butterflies and vary within families. We find differences in the shape of nipples between nocturnal and diurnal species, and that anti-glow reflectance levels are different at different wave-lengths, a result thereby contradicting the currently accepted theory of eye glow for predator avoidance. We find that there is reduced reflectance, and hence greater photon absorption, at UV light, which is probably a reason why moths are attracted to UV. We note that the effective refractive index at the end of the nipples is very close to the refractive index of water, allowing almost all the species with nipples to see without distortion when the eye is partially or completely wet and providing the potential to keep eyes dry. These observations provide a functional explanation for these arrays. Of special interest is the finding that their repeated and independent loss across lepidopteran phylogeny is inconsistent with the explanation that they are being lost in the 'higher', more active butterflies.

2.
J Control Release ; 245: 108-115, 2017 01 10.
Artículo en Inglés | MEDLINE | ID: mdl-27908758

RESUMEN

Staphylococcus aureus infections of the skin and soft tissue pose a major concern to public health, largely owing to the steadily increasing prevalence of drug resistant isolates. As an alternative mode of treatment both bacteriophage endolysins and bacteriocins have been shown to possess antimicrobial efficacy against multiple species of bacteria including otherwise drug resistant strains. Despite this, the administration and exposure of such antimicrobials should be restricted until required in order to discourage the continued evolution of bacterial resistance, whilst maintaining the activity and stability of such proteinaceous structures. Utilising the increase in skin temperature during infection, the truncated bacteriophage endolysin CHAPK and the staphylococcal bacteriocin lysostaphin have been co-administered in a thermally triggered manner from Poly(N-isopropylacrylamide) (PNIPAM) nanoparticles. The thermoresponsive nature of the PNIPAM polymer has been employed in order to achieve the controlled expulsion of a synergistic enzybiotic cocktail consisting of CHAPK and lysostaphin. The point at which this occurs is modifiable, in this case corresponding to the threshold temperature associated with an infected wound. Consequently, bacterial lysis was observed at 37°C, whilst growth was maintained at the uninfected skin temperature of 32°C.


Asunto(s)
Antibacterianos/administración & dosificación , Bacteriocinas/administración & dosificación , Endopeptidasas/administración & dosificación , Lisostafina/administración & dosificación , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Nanopartículas/administración & dosificación , Resinas Acrílicas/administración & dosificación , Resinas Acrílicas/química , Antibacterianos/química , Bacteriocinas/química , Bacteriófagos , Endopeptidasas/química , Calor , Lisostafina/química , Staphylococcus aureus Resistente a Meticilina/ultraestructura , Pruebas de Sensibilidad Microbiana , Microscopía Electrónica de Rastreo , Nanopartículas/química , Nanopartículas/ultraestructura
3.
J Eat Disord ; 3: 29, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26279837

RESUMEN

OBJECTIVE: This systematic review aims to critically examine the existing literature that has reported on the links between aspects of religiosity, spirituality and disordered eating, psychopathology and body image concerns. METHOD: A systematic search of online databases (PsycINFO, Medline, Embase and Web of Science) was conducted in December 2014. A search protocol was designed to identify relevant articles that quantitatively explored the relationship between various aspects of religiosity and/or spirituality and disordered eating, psychopathology and/or body image concerns in non-clinical samples of women and men. RESULTS: Twenty-two studies were identified to have matched the inclusion criteria. Overall, the main findings to emerge were that strong and internalised religious beliefs coupled with having a secure and satisfying relationship with God were associated with lower levels of disordered eating, psychopathology and body image concern. Conversely, a superficial faith coupled with a doubtful and anxious relationship with God were associated with greater levels of disordered eating, psychopathology and body image concern. DISCUSSION: While the studies reviewed have a number of evident limitations in design and methodology, there is sufficient evidence to make this avenue of enquiry worth pursuing. It is hoped that the direction provided by this review will lead to further investigation into the protective benefits of religiosity and spirituality in the development of a clinical eating disorder. Thus a stronger evidence base can then be utilised in developing community awareness and programs which reduce the risk.

4.
PLoS One ; 6(4): e18899, 2011 Apr 22.
Artículo en Inglés | MEDLINE | ID: mdl-21526122

RESUMEN

Staphylococcus aureus is a commensal organism and a frequent cause of skin and soft tissue infections, which can progress to serious invasive disease. This bacterium uses its fibronectin binding proteins (FnBPs) to invade host cells and it has been hypothesised that this provides a protected niche from host antimicrobial defences, allows access to deeper tissues and provides a reservoir for persistent or recurring infections. FnBPs contain multiple tandem fibronectin-binding repeats (FnBRs) which bind fibronectin with varying affinity but it is unclear what selects for this configuration. Since both colonisation and skin infection are dependent upon the interaction of S. aureus with keratinocytes we hypothesised that this might select for FnBP function and thus composition of the FnBR region. Initial experiments revealed that S. aureus attachment to keratinocytes is rapid but does not require FnBRs. By contrast, invasion of keratinocytes was dependent upon the FnBR region and occurred via similar cellular processes to those described for endothelial cells. Despite this, keratinocyte invasion was relatively inefficient and appeared to include a lag phase, most likely due to very weak expression of α(5)ß(1) integrins. Molecular dissection of the role of the FnBR region revealed that efficient invasion of keratinocytes was dependent on the presence of at least three high-affinity (but not low-affinity) FnBRs. Over-expression of a single high-affinity or three low-affinity repeats promoted invasion but not to the same levels as S. aureus expressing an FnBPA variant containing three high-affinity repeats. In summary, invasion of keratinocytes by S. aureus requires multiple high-affinity FnBRs within FnBPA, and given the importance of the interaction between these cell types and S. aureus for both colonisation and infection, may have provided the selective pressure for the multiple binding repeats within FnBPA.


Asunto(s)
Adhesinas Bacterianas/química , Adhesinas Bacterianas/metabolismo , Queratinocitos/microbiología , Secuencias Repetitivas de Aminoácido , Staphylococcus aureus/fisiología , Adhesión Bacteriana/efectos de los fármacos , Línea Celular , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Movimiento Celular/efectos de los fármacos , Células Endoteliales/citología , Células Endoteliales/efectos de los fármacos , Células Endoteliales/microbiología , Humanos , Integrina alfa5beta1/metabolismo , Queratinocitos/citología , Queratinocitos/efectos de los fármacos , Queratinocitos/metabolismo , Péptidos/farmacología , Unión Proteica/efectos de los fármacos , Estructura Terciaria de Proteína , Proteínas Recombinantes/farmacología , Staphylococcus aureus/efectos de los fármacos , Factores de Tiempo
5.
BMC Microbiol ; 10: 141, 2010 May 12.
Artículo en Inglés | MEDLINE | ID: mdl-20462430

RESUMEN

BACKGROUND: Photorhabdus are Gram-negative nematode-symbiotic and insect-pathogenic bacteria. The species Photorhabdus asymbiotica is able to infect humans as well as insects. We investigated the secreted proteome of a clinical isolate of P. asymbiotica at different temperatures in order to identify proteins relevant to the infection of the two different hosts. RESULTS: A comparison of the proteins secreted by a clinical isolate of P. asymbiotica at simulated insect (28 degrees C) and human (37 degrees C) temperatures led to the identification of a small and highly abundant protein, designated Pam, that is only secreted at the lower temperature. The pam gene is present in all Photorhabdus strains tested and shows a high level of conservation across the whole genus, suggesting it is both ancestral to the genus and probably important to the biology of the bacterium. The Pam protein shows limited sequence similarity to the 13.6 kDa component of a binary toxin of Bacillus thuringiensis. Nevertheless, injection or feeding of heterologously produced Pam showed no insecticidal activity to either Galleria mellonella or Manduca sexta larvae. In bacterial colonies, Pam is associated with an extracellular polysaccharide (EPS)-like matrix, and modifies the ability of wild-type cells to attach to an artificial surface. Interestingly, Surface Plasmon Resonance (SPR) binding studies revealed that the Pam protein itself has adhesive properties. Although Pam is produced throughout insect infection, genetic knockout does not affect either insect virulence or the ability of P. luminescens to form a symbiotic association with its host nematode, Heterorhabditis bacteriophora. CONCLUSIONS: We studied a highly abundant protein, Pam, which is secreted in a temperature-dependent manner in P. asymbiotica. Our findings indicate that Pam plays an important role in enhancing surface attachment in insect blood. Its association with exopolysaccharide suggests it may exert its effect through mediation of EPS properties. Despite its abundance and conservation in the genus, we find no evidence for a role of Pam in either virulence or symbiosis.


Asunto(s)
Adhesinas Bacterianas/metabolismo , Adhesión Bacteriana , Proteínas Bacterianas/metabolismo , Photorhabdus/fisiología , Polisacáridos Bacterianos/metabolismo , Adhesinas Bacterianas/genética , Animales , Bacillus thuringiensis/genética , Proteínas Bacterianas/genética , Toxinas Bacterianas/genética , Electroforesis en Gel Bidimensional , Infecciones por Bacterias Gramnegativas/microbiología , Humanos , Lepidópteros/microbiología , Nematodos/microbiología , Photorhabdus/crecimiento & desarrollo , Photorhabdus/aislamiento & purificación , Photorhabdus/patogenicidad , Proteoma/análisis , Homología de Secuencia de Aminoácido , Resonancia por Plasmón de Superficie , Simbiosis , Temperatura , Virulencia
6.
Australas Psychiatry ; 17(5): 380-4, 2009.
Artículo en Inglés | MEDLINE | ID: mdl-20455798

RESUMEN

OBJECTIVE: The aim of this paper is to investigate the relationship between puberty and the onset of green-sickness in early modern popular culture with findings on puberty and the onset of anorexia nervosa. METHOD: Four plays from the late 16th and early 17th centuries dealing with 14-15-year-old girls were analysed for information on puberty and for precipitating factors for the onset of green-sickness. These were then contrasted with the literature on anorexia nervosa where puberty has been proposed as a potential risk factor for the development of the disorder. RESULTS: These four plays endorse a common understanding in early modern popular culture that puberty presents a major developmental challenge for young women. Four common precipitating factors for the development of green-sickness were identified: socio-economic status; age at onset; nutrition and sexual development. These have a striking resemblance to our current understanding of the development of anorexia nervosa in adolescence. CONCLUSION: Green-sickness shares certain similarities with anorexia nervosa. It is a post menarcheal condition arising out of the body's ripeness for reproduction at around the age of 14. Affluence and improved nutrition were recognized as contributory factors. The development of both green-sickness and anorexia nervosa appear to share a common theme in the context of puberty.


Asunto(s)
Anemia Hipocrómica/historia , Anorexia Nerviosa/historia , Drama , Historia Medieval , Literatura Medieval , Pubertad/psicología , Adolescente , Conducta del Adolescente , Factores de Edad , Anemia Hipocrómica/psicología , Anorexia Nerviosa/psicología , Femenino , Historia del Siglo XVI , Historia del Siglo XVII , Humanos , Menarquia/psicología , Desarrollo Sexual , Maduración Sexual
7.
Mycol Res ; 111(Pt 2): 240-9, 2007 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-17324760

RESUMEN

A locust wing bioassay, that allowed an entomopathogenic fungus to be removed from host cuticle before penetration, was used to investigate the role of surface lipids and waxes in pre-penetration growth of the specific locust pathogen Metarhizium anisopliae var. acridum. SEM and atomic force electron microscopy showed the impact of the fungus on the architecture of the cuticle surface. Although the fungus can germinate on authentic alkanes as the sole carbon source, only low levels of germination occurred on crude, non-polar wing cuticle extracts, containing a mixture of long-chain n-alkanes and other waxes (identified in particular by gas chromatography and mass spectroscopy). The fungus removed a large proportion of non-polar and polar components during pre-penetration growth on the wing. Polar crude extracts from Schistocerca gregaria hindwings, which contained fatty acids, fatty acid esters, glucose, amino acids and peptides, were strong promoters of germination, and poor germination was observed on a locust hindwing from which the extract had been taken. Thus simple polar compounds, also present on the surface, may be required to stimulate germination before the fungus can make use of a complex mixture of non-polar lipids.


Asunto(s)
Saltamontes/microbiología , Metarhizium/crecimiento & desarrollo , Ceras/metabolismo , Animales , Cromatografía de Gases y Espectrometría de Masas , Hidrocarburos/metabolismo , Metarhizium/metabolismo , Metarhizium/ultraestructura , Microscopía de Fuerza Atómica , Microscopía Electrónica de Rastreo , Ceras/análisis , Alas de Animales/microbiología
8.
J Control Release ; 95(3): 557-66, 2004 Mar 24.
Artículo en Inglés | MEDLINE | ID: mdl-15023466

RESUMEN

Non-ionic surfactants have been employed as alternatives to PVA for the emulsification-encapsulation of a conformationally labile protein (FIII9'-10) into PLGA microspheres. FIII9'-10 was encapsulated using a w/o/w double emulsification-evaporation technique and the microspheres fabricated were characterized by SEM and CLSM. The peptide backbone integrity of FIII9'-10 was assayed by SDS-PAGE and the degree of unfolding of FIII9'-10 following emulsification-encapsulation was assessed using a fibroblast cell-attachment assay. The encapsulation efficiency for FIII9'-10 was 25% when using PVA, compared to 50-60% when using Igepal CA-630 or Triton-X100, with values below for the other surfactants. FIII9'-10 released from microspheres promoted cell attachment in a concentration-dependent manner, only Igepal CA-630 and Triton X-100 maintaining near-maximal cell attachment, indicating that the conformation of the relatively unstable FIII9' domain was preserved. All non-ionic surfactants reduced microsphere surface porosity, compared to PVA, and an increasing surface rugosity (leading to minor 'ridges') could be correlated with decreasing surfactant HLB. Low surface porosities did not effect the diffusion of FIII9'-10 from the microspheres' internal pores in a 'burst release', as may have been imagined. In summary, non-ionic surfactants should be considered over PVA for the maintenance of biological activity of conformationally labile proteins during encapsulation.


Asunto(s)
Fibronectinas/farmacocinética , Microesferas , Polímeros/farmacocinética , Animales , Sitios de Unión/efectos de los fármacos , Bovinos , Preparaciones de Acción Retardada/química , Preparaciones de Acción Retardada/farmacocinética , Fibronectinas/química , Ácido Láctico/química , Ácido Láctico/farmacocinética , Microscopía Confocal/métodos , Microscopía Electrónica de Rastreo/métodos , Octoxinol , Tamaño de la Partícula , Polietilenglicoles/química , Polietilenglicoles/farmacocinética , Ácido Poliglicólico/química , Ácido Poliglicólico/farmacocinética , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Polímeros/química , Alcohol Polivinílico/química , Alcohol Polivinílico/farmacocinética , Conformación Proteica/efectos de los fármacos , Albúmina Sérica Bovina/química , Tensoactivos/química , Tensoactivos/farmacocinética , Tecnología Farmacéutica/métodos
9.
Cell Microbiol ; 4(6): 329-39, 2002 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-12067318

RESUMEN

Invertebrates, including insects, are being developed as model systems for the study of bacterial virulence. However, we understand little of the interaction between bacteria and specific invertebrate tissues or the immune system. To establish an infection model for Photorhabdus, which is released directly into the insect blood system by its nematode symbiont, we document the number and location of recoverable bacteria found during infection of Manduca sexta. After injection into the insect larva, P. luminescens multiplies in both the midgut and haemolymph, only later colonizing the fat body and the remaining tissues of the cadaver. Bacteria persist by suppressing haemocyte-mediated phagocytosis and culture supernatants grown in vitro, as well as plasma from infected insects, suppress phagocytosis of P. luminescens. Using GFP-labelled bacteria, we show that colonization of the gut begins at the anterior of the midgut and proceeds posteriorly. Within the midgut, P. luminescens occupies a specific niche between the extracellular matrix and basal membrane (lamina) of the folded midgut epithelium. Here, the bacteria express the gut-active Toxin complex A (Tca) and an RTX-like metalloprotease PrtA. This close association of the bacteria with the gut, and the production of toxins and protease, triggers a massive programmed cell death of the midgut epithelium.


Asunto(s)
Manduca/microbiología , Photorhabdus/patogenicidad , Animales , Apoptosis , Toxinas Bacterianas/biosíntesis , Sistema Digestivo/microbiología , Sistema Digestivo/ultraestructura , Modelos Animales de Enfermedad , Relación Dosis-Respuesta Inmunológica , Larva/crecimiento & desarrollo , Larva/microbiología , Manduca/citología , Manduca/crecimiento & desarrollo , Metaloendopeptidasas/biosíntesis , Metaloendopeptidasas/toxicidad , Microscopía Electrónica/métodos , Fagocitosis/inmunología , Photorhabdus/crecimiento & desarrollo , Virulencia/inmunología
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