Phenotypic variation in the molt characteristics of a seasonal coat color-changing species reveals limited resilience to climate change.

The snowshoe hare (Lepus americanus) possesses a broad suite of adaptations to winter, including a seasonal coat color molt. Recently, climate change has been implicated in the range contraction of snowshoe hares along the southern range boundary. With shortening snow season duration, snowshoe hares are experiencing increased camouflage mismatch with their environment reducing survival. Phenological variation of hare molt at regional scales could facilitate local adaptation in the face of climate change, but the level of variation, especially along the southern range boundary, is unknown. Using a network of trail cameras and historical museum specimens, we (1) developed contemporary and historical molt phenology curves in the Upper Great Lakes region, USA, (2) calculated molt rate and variability in and among populations, and (3) quantified the relationship of molt characteristics to environmental conditions for snowshoe hares across North America. We found that snowshoe hares across the region exhibited similar fall and spring molt phenologies, rates and variation. Yet, an insular island population of hares on Isle Royale National Park, MI, completed their molt a week earlier in the fall and initiated molt almost 2 weeks later in the spring as well as exhibited slower rates of molting in the fall season compared to the mainland. Over the last 100 years, snowshoe hares across the region have not shifted in fall molt timing; though contemporary spring molt appears to have advanced by 17 days (~ 4 days per decade) compared to historical molt phenology. Our research indicates that some variation in molt phenology exists for snowshoe hares in the Upper Great Lakes region, but whether this variation is enough to offset the consequences of climate change remains to be seen.

Peatland microbial community responses to plant functional group and drought are depth-dependent.

Peatlands store one-third of Earth's soil carbon, the stability of which is uncertain due to climate change-driven shifts in hydrology and vegetation, and consequent impacts on microbial communities that mediate decomposition. Peatland carbon cycling varies over steep physicochemical gradients characterizing vertical peat profiles. However, it is unclear how drought-mediated changes in plant functional groups (PFGs) and water table (WT) levels affect microbial communities at different depths. We combined a multiyear mesocosm experiment with community sequencing across a 70-cm depth gradient, to test the hypotheses that vascular PFGs (Ericaceae vs. sedges) and WT (high vs. low) structure peatland microbial communities in depth-dependent ways. Several key results emerged. (i) Both fungal and prokaryote (bacteria and archaea) community structure shifted with WT and PFG manipulation, but fungi were much more sensitive to PFG whereas prokaryotes were much more sensitive to WT. (ii) PFG effects were largely driven by Ericaceae, although sedge effects were evident in specific cases (e.g., methanotrophs). (iii) Treatment effects varied with depth: the influence of PFG was strongest in shallow peat (0-10, 10-20 cm), whereas WT effects were strongest at the surface and middle depths (0-10, 30-40 cm), and all treatment effects waned in the deepest peat (60-70 cm). Our results underline the depth-dependent and taxon-specific ways that plant communities and hydrologic variability shape peatland microbial communities, pointing to the importance of understanding how these factors integrate across soil profiles when examining peatland responses to climate change.

Impacts of experimental alteration of water table regime and vascular plant community composition on peat mercury profiles and methylmercury production.

Climate change is expected to alter the hydrology and vascular plant communities in peatland ecosystems. These changes may have as yet unexplored impacts on peat mercury (Hg) concentrations and net methylmercury (MeHg) production. In this study, peat was collected from PEATcosm, an outdoor, controlled mesocosm experiment where peatland water table regimes and vascular plant functional groups were manipulated over several years to simulate potential climate change effects. Potential Hg(II) methylation and MeHg demethylation rate constants were assessed using enriched stable isotope incubations at the end of the study in 2015, and ambient peat total Hg (THg) and MeHg concentration depth profiles were tracked annually from 2011 to 2014. Peat THg and MeHg concentrations and the proportion of THg methylated (%MeHg) increased significantly within the zone of water table fluctuation when water tables were lowered, but potential Hg(II) methylation rate constants were similar regardless of water table treatment. When sedges dominate over ericaceous shrubs, MeHg concentrations and %MeHg became significantly elevated within the sedge rooting zone. Increased desorption of Hg(II) and MeHg from the solid phase peat into pore water occurred with a lowered water table and predominant sedge cover, likely due to greater aerobic peat decomposition. Deeper, more variable water tables and a transition to sedge-dominated communities coincided with increased MeHg accumulation within the zone of water table fluctuation. Sustained high water tables promoted the net downward migration of Hg(II) and MeHg. The simultaneous decrease in Hg(II) and MeHg concentrations in the near-surface peat and accumulation deeper in the peat profile, combined with the trends in Hg(II) and MeHg partitioning to mobile pore waters, suggest that changes to peatland hydrology and vascular plant functional groups redistribute peat Hg(II) and MeHg via vertical hydrochemical transport mechanisms.

Patterns and drivers of fungal community depth stratification in Sphagnum peat.

Peatlands store an immense pool of soil carbon vulnerable to microbial oxidation due to drought and intentional draining. We used amplicon sequencing and quantitative PCR to (i) examine how fungi are influenced by depth in the peat profile, water table and plant functional group at the onset of a multiyear mesocosm experiment, and (ii) test if fungi are correlated with abiotic variables of peat and pore water. We hypothesized that each factor influenced fungi, but that depth would have the strongest effect early in the experiment. We found that (i) communities were strongly depth stratified; fungi were four times more abundant in the upper (10-20 cm) than the lower (30-40 cm) depth, and dominance shifted from ericoid mycorrhizal fungi to saprotrophs and endophytes with increasing depth; (ii) the influence of plant functional group was depth dependent, with Ericaceae structuring the community in the upper peat only; (iii) water table had minor influences; and (iv) communities strongly covaried with abiotic variables, including indices of peat and pore water carbon quality. Our results highlight the importance of vertical stratification to peatland fungi, and the depth dependency of plant functional group effects, which must be considered when elucidating the role of fungi in peatland carbon dynamics.

Fertility-dependent effects of ectomycorrhizal fungal communities on white spruce seedling nutrition.

Ectomycorrhizal fungi (EcMF) typically colonize nursery seedlings, but nutritional and growth effects of these communities are only partly understood. To examine these effects, Picea glauca seedlings collected from a tree nursery naturally colonized by three dominant EcMF were divided between fertilized and unfertilized treatments. After one growing season seedlings were harvested, ectomycorrhizas identified using DNA sequencing, and seedlings analyzed for leaf nutrient concentration and content, and biomass parameters. EcMF community structure-nutrient interactions were tested using nonmetric multidimensional scaling (NMDS) combined with vector analysis of foliar nutrients and biomass. We identified three dominant species: Amphinema sp., Atheliaceae sp., and Thelephora terrestris. NMDS + envfit revealed significant community effects on seedling nutrition that differed with fertilization treatment. PERMANOVA and regression analyses uncovered significant species effects on host nutrient concentration, content, and stoichiometry. Amphinema sp. had a significant positive effect on phosphorus (P), calcium and zinc concentration, and P content; in contrast, T. terrestris had a negative effect on P concentration. In the unfertilized treatment, percent abundance of the Amphinema sp. negatively affected foliar nitrogen (N) concentration but not content, and reduced foliar N/P. In fertilized seedlings, Amphinema sp. was positively related to foliar concentrations of N, magnesium, and boron, and both concentration and content of manganese, and Atheliaceae sp. had a negative relationship with P content. Findings shed light on the community and species effects on seedling condition, revealing clear functional differences among dominants. The approach used should be scalable to explore function in more complex communities composed of unculturable EcMF.

Association of Pinus banksiana Lamb. and Populus tremuloides Michx. seedling fine roots with Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina).

Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina, Hydanaceae), commonly regarded as a wood decay fungus, was consistently isolated from bareroot nursery Pinus banksiana Lamb. seedlings. S. brinkmannii was found in ectomycorrhizae formed by Thelephora terrestris Ehrh., Laccaria laccata (Scop.) Cooke, and Suillus luteus (L.) Roussel. In pure culture combinations with sterile P. banksiana and Populus tremuloides Michx. seedlings, S. brinkmannii colonized root cortical cells while not killing seedlings. Colonization by S. brinkmannii appeared to be intracellular but typical endo- or ectomycorrhizae were not formed. The fungus did not decay roots, although it was shown to produce cellulase in enzyme tests. Results suggest a unique association between S. brinkmannii and seedling roots that is neither mycorrhizal nor detrimental; its exact function remains to be elucidated.