RESUMEN
Understanding the local atomic order in amorphous thin film coatings and how it relates to macroscopic performance factors, such as mechanical loss, provides an important path towards enabling the accelerated discovery and development of improved coatings. High precision x-ray scattering measurements of thin films of amorphous zirconia-doped tantala (ZrO_{2}-Ta_{2}O_{5}) show systematic changes in intermediate range order (IRO) as a function of postdeposition heat treatment (annealing). Atomic modeling captures and explains these changes, and shows that the material has building blocks of metal-centered polyhedra and the effect of annealing is to alter the connections between the polyhedra. The observed changes in IRO are associated with a shift in the ratio of corner-sharing to edge-sharing polyhedra. These changes correlate with changes in mechanical loss upon annealing, and suggest that the mechanical loss can be reduced by developing a material with a designed ratio of corner-sharing to edge-sharing polyhedra.
RESUMEN
Screening for human papillomavirus (HPV) integration into host cell chromosomes typically requires large amounts of time and reagents. We developed a rapid and sensitive assay based on exonuclease V (ExoV) and quantitative polymerase chain reaction (qPCR) to determine HPV genome configurations in cell lines and tissues. We established the assay using genomic DNA from cell lines known to harbor integrated or episomal HPV16. DNA was incubated with ExoV, which is specific for linear DNA, and the DNA fraction resistant to digestion was measured by qPCR. The percent of DNA resistant to ExoV digestion was calculated relative to undigested DNA for determination of episomal or integrated HPV16. The ExoV assay was accurate, capable of distinguishing episomal from integrated HPV16 in cell lines and tissues. Future applications of the ExoV assay may include screening of HPV genome configurations in the progression of HPV-associated cancers.
Asunto(s)
ADN Viral/análisis , Exodesoxirribonucleasa V/metabolismo , Papillomavirus Humano 16/genética , Plásmidos , Provirus/genética , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Integración Viral , Células Cultivadas , ADN Viral/genética , Papillomavirus Humano 16/crecimiento & desarrollo , HumanosRESUMEN
In a microcalorimetric neutrino mass experiment using the radioactive decay of (163)Ho, the radioactive material must be fully embedded in the microcalorimeter absorber. One option that is being investigated is to implant the radioactive isotope into a gold absorber, as gold is successfully used in other applications. However, knowing the thermal properties at the working temperature of microcalorimeters is critical for choosing the absorber material and for optimizing the detector performance. In particular, it is paramount to understand if implanting the radioactive material in gold changes its heat capacity. We used a bolometric technique to measure the heat capacity of gold films, implanted with various concentrations of holmium and erbium (a byproduct of the (163)Ho fabrication), in the temperature range 70 mK-300 mK. Our results show that the specific heat capacity of the gold films is not affected by the implant, making this a viable option for a future microcalorimeter holmium experiment.
RESUMEN
Refrigerators for space and other applications working around 100 mK require lightweight components with good thermal properties. We have measured the thermal properties of high-purity (99.95%) magnesium, which is five times lighter than copper, over the 60-150 mK range and found that it is well-behaved down to these temperatures. Both conductivity and heat capacity are in good agreement with extrapolations from measurements at higher temperatures. The heat capacity per unit volume is about the same as copper and the thermal conductivity about 2.7 times lower than copper of similar residual resistivity ratio, as expected from magnesium's higher room-temperature resistivity.
