Analysis of substrate specificity of cytochrome P450 monooxygenases involved in trichothecene toxin biosynthesis.

Trichothecenes are a structurally diverse family of toxic secondary metabolites produced by certain species of multiple fungal genera. All trichothecene analogs share a core 12,13-epoxytrichothec-9-ene (EPT) structure but differ in presence, absence and types of substituents attached to various positions of EPT. Formation of some of the structural diversity begins early in the biosynthetic pathway such that some producing species have few trichothecene biosynthetic intermediates in common. Cytochrome P450 monooxygenases (P450s) play critical roles in formation of trichothecene structural diversity. Within some species, relaxed substrate specificities of P450s allow individual orthologs of the enzymes to modify multiple trichothecene biosynthetic intermediates. It is not clear, however, whether the relaxed specificity extends to biosynthetic intermediates that are not produced by the species in which the orthologs originate. To address this knowledge gap, we used a mutant complementation-heterologous expression analysis to assess whether orthologs of three trichothecene biosynthetic P450s (TRI11, TRI13 and TRI22) from Fusarium sporotrichioides, Trichoderma arundinaceum, and Paramyrothecium roridum can modify trichothecene biosynthetic intermediates that they do not encounter in the organism in which they originated. The results indicate that TRI13 and TRI22 could not modify the intermediates that they do not normally encounter, whereas TRI11 could modify an intermediate that it does not normally encounter. These findings indicate that substrate promiscuity varies among trichothecene biosynthetic P450s. One structural feature that likely impacts the ability of the P450s to use biosynthetic intermediates as substrates is the presence and absence of an oxygen atom attached to carbon atom 3 of EPT.

Does alteration of fumonisin production in Fusarium verticillioides lead to volatolome variation?

Fusarium verticillioides, a major fungal pathogen of maize, produces fumonisins, mycotoxins of global food safety concern. Control practices are needed to reduce the negative health and economic impacts of fumonisins. Therefore, we investigated volatile organic compounds (VOCs) emitted by fumonisin-producing (wild-type) and nonproducing (mutant) strains of F. verticillioides. VOC emissions were analyzed by gas chromatography-mass spectrometry following inoculation of maize kernels, and fumonisin accumulation was analyzed by high-performance liquid chromatography. Mutants emitted VOCs, including ethyl 3-methylbutanoate, that the wild type did not emit. In particular, ANOVA analysis showed significant differences between mutants and wild type for 4 VOCs which emission was correlated with absence of fumonisins. Exogenous ethyl 3-methylbutanoate reduced growth and fumonisin production in wild-type F. verticillioides, showing its potential in biocontrol. Together, our findings offer valuable insights into how mycotoxin production can impact VOC emissions from F. verticillioides and reveal a potential biocontrol strategy to reduce fumonisin contamination.

How the black hole illusion environment affects operational performance at different flight phases in aviation.

The black hole illusion (BHI) is a subtype of spatial disorientation that can result in fatal consequences in aviation. Research on the BHI has generally focused on altitude deviation, and few studies have examined the effect across different flight phases. In a simulation-based experiment, 18 participants performed 12 simulated approach and landing tasks in normal and BHI environments. Flight performance was analyzed with 14 flight parameters and was compared across five points and three phases, which were referenced from a National Transportation Safety Board report and other previous studies. Results showed that multiple flight parameters were significantly impaired and that their influences varied from the initial approach to the final touchdown. In the BHI environment, participants tended to descend aggressively during the approach phase and flew a lower but similar glidepath during the last approach phase. They might have realized the abnormal situation induced by the BHI but usually were unable to recover from the dangerous maneuver in time. Additionally, the result of glide path error, one of the most commonly used variables in previous BHI research, was only significant during the last approach phase. Flight stability was also impaired in the BHI environment. This is the first study to systematically analyze the BHI effects on multiple flight parameters at different flight phases. The use of this experimental paradigm could facilitate future research to evaluate and prevent the BHI in a more comprehensive way.

Testing the saliency-based account of phasic alertness.

As an essential component of the human attention system, the effect of phasic alertness refers to the change of performance with the presence of a preceding warning signal. Weinbach and Henik (Cognition, 133 (2), 414-419, 2014) argued that phasic alertness is an adaptive mechanism that diverts attention to salient events. This mechanism enhances selective attention when the critical event is more salient than others. When selective attention to less salient details is required, phasic alertness can lead to more interference from task-irrelevant information. The experiment on which this saliency-based account of phasic alertness is based has not been replicated. In two experiments, the present study attempted to replicate the alertness-related findings of Weinbach and Henik. Although we used a similar design, the results did not reveal evidence for an interaction between phasic alertness and response congruency in the global/local processing task. Our results do not support the saliency-based account of phasic alertness. We argue that more systematic investigation is needed for this phasic alertness account.

The Simon Effect Asymmetry for Left- and Right-Dominant Persons.

When participants respond to a task-relevant stimulus attribute by pressing a left or right key with the respective index finger, reaction time is shorter if task-irrelevant left-right stimulus location corresponds to that of the response key than if it does not. For right-handers, this Simon effect is larger for right-located than left-located stimuli; for left-handers this Simon-effect asymmetry is reversed. A similar asymmetry has been found for right-footers pressing pedals with their feet. For analyses that separate stimulus- and response-location factors, these asymmetries appear as a main effect of response location, with responses being faster with the dominant effector. If the Simon-effect asymmetry is strictly a function of effector dominance, it should reverse for left-footers responding with their feet. In Experiment 1, left-dominant persons showed faster responses with the left than right hand but with the right than left foot, a finding consistent with prior research on tapping actions. Right-dominant persons also showed the right-foot asymmetry but, unexpectedly, not the typical asymmetry with hand responses. To evaluate whether hand-presses yield results distinct from finger-presses, in Experiment 2 participants performed the Simon task with finger-presses and hand-presses. The opposing asymmetries for right- and left-dominant persons were evident for both response modes. Our results are consistent with the view that the Simon effect asymmetry is primarily due to differences in effector efficiency, usually but not always favoring the dominant effector.

Vehicle crash simulations for safety: Introduction of connected and automated vehicles on the roadways.

Traffic accidents are one main cause of human fatalities in modern society. With the fast development of connected and autonomous vehicles (CAVs), there comes both challenges and opportunities in improving traffic safety on the roads. While on-road tests are limited due to their high cost and hardware requirements, simulation has been widely used to study traffic safety. To make the simulation as realistic as possible, real-world crash data such as crash reports could be leveraged in the creation of the simulation. In addition, to enable such simulations to capture the complexity of traffic, especially when both CAVs and human-driven vehicles co-exist on the road, careful consideration needs to be given to the depiction of human behaviors and control algorithms of CAVs and their interactions. In this paper, the authors reviewed literature that is closely related to crash analysis based on crash reports and to simulation of mixed traffic when CAVs and human-driven vehicles co-exist, for studying traffic safety. Three main aspects are examined based on our literature review: data source, simulation methods, and human factors. It was found that there is an abundance of research in the respective areas, namely, crash report analysis, crash simulation studies (including vehicle simulation, traffic simulation, and driving simulation), and human factors. However, there is a lack of integration between them. Future research is recommended to integrate and leverage different state-of-the-art transportation-related technologies to contribute to road safety by developing an all-in-one-step crash analysis system.

Fusarium graminearum Effector FgNls1 Targets Plant Nuclei to Induce Wheat Head Blight.

Fusarium head blight (FHB) caused by Fusarium graminearum is one of the most devastating diseases of wheat and barley worldwide. Effectors suppress host immunity and promote disease development. The genome of F. graminearum contains hundreds of effectors with unknown function. Therefore, investigations of the functions of these effectors will facilitate developing novel strategies to enhance wheat resistance to FHB. We characterized a F. graminearum effector, FgNls1, containing a signal peptide and multiple eukaryotic nuclear localization signals. A fusion protein of green fluorescent protein and FgNls1 accumulated in plant cell nuclei when transiently expressed in Nicotiana benthamiana. FgNls1 suppressed Bax-induced cell death when co-expressed in N. benthamiana. We revealed that the expression of FgNLS1 was induced in wheat spikes infected with F. graminearum. The Fgnls1 mutants significantly reduced initial infection and FHB spread within a spike. The function of FgNLS1 was restored in the Fgnls1-complemented strains. Wheat histone 2B was identified as an interacting protein by FgNls1-affinity chromatography. Furthermore, transgenic wheat plants that silence FgNLS1 expression had significantly lower FHB severity than control plants. This study demonstrates a critical role of FgNls1 in F. graminearum pathogenesis and indicates that host-induced gene silencing targeting F. graminearum effectors is a promising approach to enhance FHB resistance. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Emotional experiences of service robots' anthropomorphic appearance: a multimodal measurement method.

Anthropomorphic appearance is a key factor to affect users' attitudes and emotions. This research aimed to measure emotional experience caused by robots' anthropomorphic appearance with three levels - high, moderate, and low - using multimodal measurement. Fifty participants' physiological and eye-tracker data were recorded synchronously while they observed robot images that were displayed in random order. Afterward, the participants reported subjective emotional experiences and attitudes towards those robots. The results showed that the images of the moderately anthropomorphic service robots induced higher pleasure and arousal ratings, and yielded significantly larger pupil diameter and faster saccade velocity, than did the low or high robots. Moreover, participants' facial electromyography, skin conductance, and heart-rate responses were higher when observing moderately anthropomorphic service robots. An implication of the research is that service robots' appearance should be designed to be moderately anthropomorphic; too many human-like features or machine-like features may disturb users' positive emotions and attitudes.Practitioner Summary: This research aimed to measure emotional experience caused by three types of anthropomorphic service robots using a multimodal measurement experiment. The results showed that moderately anthropomorphic service robots evoked more positive emotion than high and low anthropomorphic robots. Too many human-like features or machine-like features may disturb users' positive emotions.

Effects of a neutral warning signal under increased temporal uncertainty.

Han and Proctor (2022a, Quarterly Journal of Experimental Psychology, 75[4], 754-764) reported that in a visual two-choice task, compared with a no-warning condition, a neutral warning tone caused shorter reaction times (RTs) but at the expense of an increase in error percentages (a speed-accuracy trade-off) at a constant 50-ms foreperiod but shorter RTs without an increase in error percentages at a 200-ms foreperiod. Also, the spatial compatibility of stimulus-response mappings was found to interact with the foreperiod effect on RT. We conducted three experiments to investigate whether these findings can be replicated without the constancy of foreperiod within a trial block. In Experiments 1 and 2, participants performed the same two-choice task as in Han and Proctor's study but with the foreperiod randomly varied among 50, 100, and 200 ms and RT feedback provided after each response. Results showed that as the foreperiod increased, RT decreased while EP increased, demonstrating a consistent speed-accuracy trade-off. Also, the mapping effect was found to be largest at the 100-ms foreperiod. In Experiment 3, RT feedback was not provided, and the warning tone speeded responses without evidence of an increase in error percentage. We conclude that the enhanced information processing at a 200-ms foreperiod depends on constancy of foreperiod within a trial block, whereas the mapping-foreperiod interaction found in Han and Proctor is relatively unaffected by increased temporal uncertainty.

Cyclopiazonic acid in soft-ripened and blue cheeses marketed in the USA.

Strains of Penicillium camemberti and P. roqueforti are used in the production of soft-ripened and blue-veined cheeses. However, some strains can produce toxic secondary metabolites (mycotoxins), including α-cyclopiazonic acid (CPA), a neurotoxin. Data on the levels of CPA in cheeses marketed in the USA are extremely limited. An enzyme-linked immunosorbent assay was adapted for measuring CPA in soft-ripened and blue-veined cheeses. Recoveries from cheese curds were 103 ± 27% (n = 30). A total of 254 samples of soft-ripened, blue and miscellaneous cheeses were examined. CPA was detected in 36/79 (45.6%) of soft-ripened cheeses and in 41/168 (24.4%) of blue-veined cheeses. Median levels in positive samples were 48.5 µg/kg and 30 µg/kg, respectively. The highest levels found were 3,820 µg/kg (in a Brie), 1,250 µg/kg (in a blue) and 7,900 µg/kg (in a Monte Enebro). The implication of such exposures is unknown, as a consensus on acceptable intake remains to be established.

Effect of Farnesol in Trichoderma Physiology and in Fungal-Plant Interaction.

Farnesol is an isoprenoid intermediate in the mevalonate (MVA) pathway and is produced by the dephosphorylation of farnesyl diphosphate. Farnesol plays a central role in cell growth and differentiation, controls production of ubiquinone and ergosterol, and participates in the regulation of filamentation and biofilm formation. Despite these important functions, studies of farnesol in filamentous fungi are limited, and information on its effects on antifungal and/or biocontrol activity is scarce. In the present article, we identified the Trichoderma harzianum gene dpp1, encoding a diacylglycerol pyrophosphatase that catalyzes production of farnesol from farnesol diphosphate. We analyzed the function of dpp1 to address the importance of farnesol in Trichoderma physiology and ecology. Overexpression of dpp1 in T. harzianum caused an expected increase in farnesol production as well as a marked change in squalene and ergosterol levels, but overexpression did not affect antifungal activity. In interaction with plants, a dpp1-overexpressing transformant acted as a sensitizing agent in that it up-regulated expression of plant defense salicylate-related genes in the presence of a fungal plant pathogen. In addition, toxicity of farnesol on Trichoderma and plants was examined. Finally, a phylogenetic study of dpp1 was performed to understand its evolutionary history as a primary metabolite gene. This article represents a step forward in the acquisition of knowledge on the role of farnesol in fungal physiology and in fungus-environment interactions.

Effects of trichothecene production by Trichoderma arundinaceum isolates from bean-field soils on the defense response, growth and development of bean plants (Phaseolus vulgaris).

The trichothecene toxin-producing fungus Trichoderma arundinaceum has potential as a biological control agent. However, most biocontrol studies have focused only on one strain, IBT 40837. In the current study, three Trichoderma isolates recovered from bean-field soils produced the trichothecene harzianum A (HA) and trichodermol, the latter being an intermediate in the HA biosynthesis. Based on phylogenetic analysis, the three isolates were assigned to the species T. arundinaceum. Their genome sequences had a high degree of similarity to the reference IBT 40837 strain, in terms of total genome size, number of predicted genes, and diversity of putative secondary metabolite biosynthetic gene clusters. HA production by these bean-field isolates conferred significant in vitro antifungal activity against Rhizoctonia solani and Sclerotinia sclerotiorum, which are some of the most important bean pathogens. Furthermore, the bean-field isolates stimulated germination of bean seeds and subsequent growth of above ground parts of the bean plant. Transcriptomic analysis of bean plants inoculated with these T. arundinaceum bean-field soil isolates indicated that HA production significantly affected expression of plant defense-related genes; this effect was particularly significant in the expression of chitinase-encoding genes. Together, these results indicate that Trichoderma species producing non-phytotoxic trichothecenes can induce defenses in plants without negatively affecting germination and development.

Age is more than just a number: The relationship among age, non-chronological age factors, self-perceived driving abilities, and autonomous vehicle acceptance.

Globally, adults aged 65 and older are a rapidly-growing population. Aging is associated with declines in perceptual, cognitive, and physical abilities, which often creates challenges in completing daily activities, such as driving. Autonomous vehicles (AVs) promise to provide older adults one way to maintain their mobility and independence. However, recent surveys of AV acceptance suggest that older adults have a lower AV acceptance compared to younger generations. One challenge is that most of these assessments have not accounted for the various non-chronological age factors that contribute to how older adults perceive their own driving skills and the utility of AVs. To fill this research gap, this study investigated the effects of non-chronological age factors and rated self-perceived driving abilities on AV acceptance across three age groups. An online survey was conducted using Amazon Mechanical Turk (MTurk), for which 438 valid responses were received. Respondents were categorized into a younger (18-40 years), middle-aged (41-64 years), and older (65-79 years) adult age group. Results showed that drivers of a younger age, with higher educational attainment, who rated themselves to have higher social support, and who have lower rated self-perceived driving abilities, report being more willing to accept AVs. Findings from this work can help to inform models of AV technology acceptance and guide in the development of marketing strategies to promote knowledge of AVs.

Change of Variable-Foreperiod Effects within an Experiment: A Bayesian Modeling Approach.

The framework of binding and retrieval in action control (BRAC) by Frings et al. (2020) proposed that repetition of any element in the previous trial triggers the retrieval of other elements in the same event file. Consistent with this framework, Los et al. (2014) argued that the temporal relation between the warning signal and the target stimulus on a trial is stored in a distinct memory trace (or, event file). Retrieval of the preceding memory trace, which is triggered by perceiving the same warning signal, leads to sequential foreperiod (SFP) effect. We modeled the data from four experiments using a Bayesian method to investigate whether the SFP effect changes over time. Results of Experiments 1, 3 and 4 support the multiple trace theory of preparation, which predicts an asymmetric sequential foreperiod effect, whereas those of Experiment 2 (extremely short foreperiods) support the repetition priming account by Capizzi et al. (2015). Moreover, the significance of the parameters showed that the asymmetry in Experiments 1 and 3 (non-aging distribution) developed gradually, whereas in Experiment 4 (uniform distribution), this asymmetry was significant from the beginning and did not change over time. Implications of these findings for temporal preparation models and BRAC framework were discussed.

Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum.

The fungus Trichoderma arundinaceum exhibits biological control activity against crop diseases caused by other fungi. Two mechanisms that likely contribute to this activity are upregulation of plant defenses and production of two types of antifungal secondary metabolites: the sesquiterpenoid harzianum A (HA) and the polyketide-derived aspinolides. The goal of the current study was to identify aspinolide biosynthetic genes as part of an effort to understand how these metabolites contribute to the biological control activity of T. arundinaceum. Comparative genomics identified two polyketide synthase genes (asp1 and asp2) that occur in T. arundinaceum and Aspergillus ochraceus, which also produces aspinolides. Gene deletion and biochemical analyses in T. arundinaceum indicated that both genes are required for aspinolide production: asp2 for formation of a 10-member lactone ring and asp1 for formation of a butenoyl subsituent at position 8 of the lactone ring. Gene expression and comparative genomics analyses indicated that asp1 and asp2 are located within a gene cluster that occurs in both T. arundinaceum and A. ochraceus. A survey of genome sequences representing 35 phylogenetically diverse Trichoderma species revealed that intact homologs of the cluster occurred in only two other species, which also produced aspinolides. An asp2 mutant inhibited fungal growth more than the wild type, but an asp1 mutant did not, and the greater inhibition by the asp2 mutant coincided with increased HA production. These findings indicate that asp1 and asp2 are aspinolide biosynthetic genes and that loss of either aspinolide or HA production in T. arundinaceum can be accompanied by increased production of the other metabolite(s). KEY POINTS: • Two polyketide synthase genes are required for aspinolide biosynthesis. • Blocking aspinolide production increases production of the terpenoid harzianum A. • Aspinolides and harzianum A act redundantly in antibiosis of T. arundinaceum.

Characterization of Host-Specific Genes from Pine- and Grass-Associated Species of the Fusarium fujikuroi Species Complex.

The Fusarium fujikuroi species complex (FFSC) includes socioeconomically important pathogens that cause disease for numerous crops and synthesize a variety of secondary metabolites that can contaminate feedstocks and food. Here, we used comparative genomics to elucidate processes underlying the ability of pine-associated and grass-associated FFSC species to colonize tissues of their respective plant hosts. We characterized the identity, possible functions, evolutionary origins, and chromosomal positions of the host-range-associated genes encoded by the two groups of fungi. The 72 and 47 genes identified as unique to the respective genome groups were potentially involved in diverse processes, ranging from transcription, regulation, and substrate transport through to virulence/pathogenicity. Most genes arose early during the evolution of Fusarium/FFSC and were only subsequently retained in some lineages, while some had origins outside Fusarium. Although differences in the densities of these genes were especially noticeable on the conditionally dispensable chromosome of F. temperatum (representing the grass-associates) and F. circinatum (representing the pine-associates), the host-range-associated genes tended to be located towards the subtelomeric regions of chromosomes. Taken together, these results demonstrate that multiple mechanisms drive the emergence of genes in the grass- and pine-associated FFSC taxa examined. It also highlighted the diversity of the molecular processes potentially underlying niche-specificity in these and other Fusarium species.

A neglected pioneer of psychology: Otto Selz's contribution to the psychology of thinking and the dispute with Gestalt psychologists in Psychological Research/Psychologische Forschung.

In the early decades of the twentieth century, Psychologische Forschung was primarily an outlet for researchers from the school of Gestalt psychology. Otto Selz, whose views were closer to those adopted in the cognitive/information-processing revolution in psychology that began in the 1950s, never published in Psychologische Forschung. However, his work was the subject of a negative evaluation in the journal in a book review by Wilhelm Benary, which was followed by critical assessments published elsewhere by Selz and Karl Bühler of a chapter of Kurt Koffka's. A lengthy rebuttal from Koffka then appeared in Psychologische Forschung. In the present paper, we describe Selz's system and Benary's assessment of it. We then explain the relevant aspects of Koffka's book chapter (in: Dessoir M (ed) Die Philosophie in ihren Einzelgebieten. Ullstein, Berlin, 1925) and the strong critiques of it by Bühler and Selz in 1926, followed by details of Koffka's (Psychol Forsch 9:163-183, 1927) response. This part of the history of psychology is of significance to contemporary psychology on several levels. We have embedded this episode against the historical backdrop of Selz's life and tragic end.

A diffusion model for the congruency sequence effect.

Two-choice reaction tasks for which stimuli differ on irrelevant and relevant dimensions (e.g., Simon, flanker, and Stroop tasks) show congruency effects. The diffusion model for conflict tasks (DMC) has provided a quantitative account of the mechanisms underlying decisions in such conflict tasks, but it has not been applied to the congruency sequence effect (CSE) for which the congruency on the prior trial influences performance on the current trial. The present study expands analysis of the reaction time (RT) distributions reflected by delta plots to the CSE, and then extends the DMC to simulate the results. With increasing RT: (1) the spatial Simon effect was almost unchanged following congruent trials but initially became smaller and finally reversed following incongruent trials; (2) the arrow-based Simon effects increased following both congruent and incongruent trials, but more so for the former than the latter; (3) the flanker congruency effect varied quadratically following congruent trials but increased linearly following incongruent trials. These results were modeled by the CSE-DMC, extended from the DMC with two additional assumptions: (1) feature integration influences only the controlled processes; (2) following incongruent trials, the automatic process is weakened. The results fit better with the CSE-DMC than with two variants that separately had only one of the two additional assumptions. These findings indicate that the CSEs for different conflict tasks have disparate RT distributions and that these disparities are likely due to the controlled and automatic processes being influenced differently for each trial sequence.

Fusarium abutilonis and F. guadeloupense, two novel species in the Fusarium buharicum clade supported by multilocus molecular phylogenetic analyses.

This study was conducted to elucidate evolutionary relationships and species diversity within the Fusarium buharicum species complex (FBSC). We also evaluate the potential of these species to produce mycotoxins and other bioactive secondary metabolites. Maximum likelihood and maximum parsimony analyses of sequences from portions of four marker loci (ITS rDNA, TEF1, RPB1, and RPB2) and the combined 4495 bp data set support recognition of seven genealogically exclusive species within the FBSC. Two of the three newly discovered species are formally described as F. abutilonis and F. guadeloupense based on concordance of gene genealogies and morphological data. Fusarium abutilonis induces leaf, stem, and root lesions on several weedy Malvaceae (Abution theophrasti, Anoda cristata, Sida spinosa) and a fabaceous host (Senna obtusifolia) in North America and also was recovered from soil in New Caledonia. Fusarium abutilonis, together with its unnamed sister, Fusarium sp. ex common marsh mallow (Hibiscus moscheutos) from Washington state, and F. buharicum pathogenic to cotton and kenaf in Russia and Iran, respectively, were strongly supported as a clade of malvaceous pathogens. The four other species of the FBSC are not known to be phytopathogenic; however, F. guadeloupense was isolated from human blood in Texas and soil in Guadeloupe. The former isolate is unique because it represents the only known case of a fusarial infection disseminated hematogenously by a species lacking microconidia and the only documented fusariosis caused by a member of the FBSC. Whole genome sequence data and extracts of cracked maize kernel cultures were analyzed to assess the potential of FBSC isolates to produce mycotoxins, pigments, and phytohormones.