Modified Carbon Nanotubes Favor Fibroblast Growth by Tuning the Cell Membrane Potential.

As is known, carbon nanotubes favor cell growth in vitro, although the underlying mechanisms are not yet fully elucidated. In this study, we explore the hypothesis that electrostatic fields generated at the interface between nonexcitable cells and appropriate scaffold might favor cell growth by tuning their membrane potential. We focused on primary human fibroblasts grown on electrospun polymer fibers (poly(lactic acid)─PLA) with embedded multiwall carbon nanotubes (MWCNTs). The MWCNTs were functionalized with either the p-methoxyphenyl (PhOME) or the p-acetylphenyl (PhCOMe) moiety, both of which allowed uniform dispersion in a solvent, good mixing with PLA and the consequent smooth and homogeneous electrospinning process. The inclusion of the electrically conductive MWCNTs in the insulating PLA matrix resulted in differences in the surface potential of the fibers. Both PLA and PLA/MWCNT fiber samples were found to be biocompatible. The main features of fibroblasts cultured on different substrates were characterized by scanning electron microscopy, immunocytochemistry, Rt-qPCR, and electrophysiology revealing that fibroblasts grown on PLA/MWCNT reached a healthier state as compared to pure PLA. In particular, we observed physiological spreading, attachment, and Vmem of fibroblasts on PLA/MWCNT. Interestingly, the electrical functionalization of the scaffold resulted in a more suitable extracellular environment for the correct biofunctionality of these nonexcitable cells. Finally, numerical simulations were also performed in order to understand the mechanism behind the different cell behavior when grown either on PLA or PLA/MWCNT samples. The results show a clear effect on the cell membrane potential, depending on the underlying substrate.

Swarm of lightsail nanosatellites for Solar System exploration.

This paper presents a study for the realization of a space mission which employs nanosatellites driven by an external laser source impinging on an optimized lightsail, as a valuable technology to launch swarms of spacecrafts into the Solar System. Nanosatellites propelled by laser can be useful for heliosphere exploration and for planetary observation, if suitably equipped with sensors, or be adopted for the establishment of network systems when placed into specific orbits. By varying the area-to-mass ratio (i.e. the ratio between the sail area and the payload weight) and the laser power, it is possible to insert nanosatellites into different hyperbolic orbits with respect to Earth, thus reaching the target by means of controlled trajectories in a relatively short amount of time. A mission involving nanosatellites of the order of 1 kg of mass is envisioned, by describing all the on-board subsystems and satisfying all the requirements in terms of power and mass budget. Particular attention is paid to the telecommunication subsystem, which must offer all the necessary functionalities. To fabricate the lightsail, the thin films technology has been considered, by verifying the sail's thermal stability during the thrust phase. Moreover, the problem of mechanical stability of the lightsail has been tackled, showing that the distance between the ligthsail structure and the payload plays a pivotal role. Some potential applications of the proposed technology are discussed, such as the mapping of the heliospheric environment.

Sustainable Synthesis of Sulfur-Single Walled Carbon Nanohorns Composite for Long Cycle Life Lithium-Sulfur Battery.

Lithium-sulfur batteries are considered one of the most appealing technologies for next-generation energy-storage devices. However, the main issues impeding market breakthrough are the insulating property of sulfur and the lithium-polysulfide shuttle effect, which cause premature cell failure. To face this challenge, we employed an easy and sustainable evaporation method enabling the encapsulation of elemental sulfur within carbon nanohorns as hosting material. This synthesis process resulted in a morphology capable of ameliorating the shuttle effect and improving the electrode conductivity. The electrochemical characterization of the sulfur-carbon nanohorns active material revealed a remarkable cycle life of 800 cycles with a stable capacity of 520 mA h/g for the first 400 cycles at C/4, while reaching a value around 300 mAh/g at the 750th cycle. These results suggest sulfur-carbon nanohorn active material as a potential candidate for next-generation battery technology.

Electrostatic polarization fields trigger glioblastoma stem cell differentiation.

Over the last few years it has been understood that the interface between living cells and the underlying materials can be a powerful tool to manipulate cell functions. In this study, we explore the hypothesis that the electrical cell/material interface can regulate the differentiation of cancer stem-like cells (CSCs). Electrospun polymer fibres, either polyamide 66 or poly(lactic acid), with embedded graphene nanoplatelets (GnPs), have been fabricated as CSC scaffolds, providing both the 3D microenvironment and a suitable electrical environment favorable for CSCs adhesion, growth and differentiation. We have investigated the impact of these scaffolds on the morphological, immunostaining and electrophysiological properties of CSCs extracted from human glioblastoma multiform (GBM) tumor cell line. Our data provide evidence in favor of the ability of GnP-incorporating scaffolds to promote CSC differentiation to the glial phenotype. Numerical simulations support the hypothesis that the electrical interface promotes the hyperpolarization of the cell membrane potential, thus triggering the CSC differentiation. We propose that the electrical cell/material interface can regulate endogenous bioelectrical cues, through the membrane potential manipulation, resulting in the differentiation of CSCs. Material-induced differentiation of stem cells and particularly of CSCs, can open new horizons in tissue engineering and new approaches to cancer treatment, especially GBM.

Ultrafast Plasmonics Beyond the Perturbative Regime: Breaking the Electronic-Optical Dynamics Correspondence.

The transient optical response of plasmonic nanostructures has recently been the focus of extensive research. Accurate prediction of the ultrafast dynamics following excitation of hot electrons by ultrashort laser pulses is of major relevance in a variety of contexts from the study of light harvesting and photocatalytic processes to nonlinear nanophotonics and the all-optical modulation of light. So far, all studies have assumed the correspondence between the temporal evolution of the dynamic optical signal, retrieved by transient absorption spectroscopy, and that of the photoexcited hot electrons, described in terms of their temperature. Here, we show both theoretically and experimentally that this correspondence does not hold under a nonperturbative excitation regime. Our results indicate that the main mechanism responsible for the breaking of the correspondence between electronic and optical dynamics is universal in plasmonics, being dominated by the nonlinear smearing of the Fermi-Dirac occupation probability at high hot-electron temperatures.

Photonic Cavity Effects for Enhanced Efficiency in Layered Perovskite-Based Light-Emitting Diodes.

Layered architectures for light-emitting diodes (LEDs) are the standard approach for solution-processable materials such as metal-halide perovskites. Upon designing the composition and thicknesses of the layers forming the LED, the primary focus is typically on the optimization of charge injection and balance. However, this approach only considers the process until electrons and holes recombine to generate photons, while for achieving optimized LED performance, the generated light must also be efficiently outcoupled. Our work focuses on the latter aspect. We assume efficient photon generation and analyze the effects of the geometrical configuration together with the dipole orientation, mimicking the light emission, on the main characteristics defining the LED, such as the Purcell effect and the outcoupling efficiency. We find that in-plane dipoles result in significantly increased outcoupling efficiency. Furthermore, the mismatch in refractive index among the layers and their different thicknesses can be tuned to maximize the Purcell effect and minimize internal losses. The combined optimization of dipole orientation and layer thicknesses can improve the efficiency of the LED up to a factor 10, hence highlighting the importance of considering also the photonic properties of the LED structures if the objective is to maximize the LED performance.

Nanoporous Metals: From Plasmonic Properties to Applications in Enhanced Spectroscopy and Photocatalysis.

The field of plasmonics is capable of enabling interesting applications in different wavelength ranges, spanning from the ultraviolet up to the infrared. The choice of plasmonic material and how the material is nanostructured has significant implications for ultimate performance of any plasmonic device. Artificially designed nanoporous metals (NPMs) have interesting material properties including large specific surface area, distinctive optical properties, high electrical conductivity, and reduced stiffness, implying their potentials for many applications. This paper reviews the wide range of available nanoporous metals (such as Au, Ag, Cu, Al, Mg, and Pt), mainly focusing on their properties as plasmonic materials. While extensive reports on the use and characterization of NPMs exist, a detailed discussion on their connection with surface plasmons and enhanced spectroscopies as well as photocatalysis is missing. Here, we report on different metals investigated, from the most used nanoporous gold to mixed metal compounds, and discuss each of these plasmonic materials' suitability for a range of structural design and applications. Finally, we discuss the potentials and limitations of the traditional and alternative plasmonic materials for applications in enhanced spectroscopy and photocatalysis.

All-Optically Reconfigurable Plasmonic Metagrating for Ultrafast Diffraction Management.

Hot-electron dynamics taking place in nanostructured materials upon irradiation with fs-laser pulses has been the subject of intensive research, leading to the emerging field of ultrafast nanophotonics. However, the most common description of nonlinear interaction with ultrashort laser pulses assumes a homogeneous spatial distribution for the photogenerated carriers. Here we theoretically show that the inhomogeneous evolution of the hot carriers at the nanoscale can disclose unprecedented opportunities for ultrafast diffraction management. In particular, we design a highly symmetric plasmonic metagrating capable of a transient symmetry breaking driven by hot electrons. The subsequent power imbalance between symmetrical diffraction orders is calculated to exceed 20% under moderate (∼2 mJ/cm2) laser fluence. Our theoretical investigation also indicates that the recovery time of the symmetric configuration can be controlled by tuning the geometry of the metaatom, and can be as fast as 2 ps for electrically connected configurations.

Tuning temperature gradients in subwavelength plasmonic nanocones with tilted illumination.

Inducing and controlling temperature gradients in illuminated subwavelength plasmonic structures is a challenging task. Here, we present a strategy to remotely induce and tune temperature gradients in a subwavelength metallic nanocone by adjusting the angle of incidence of linearly polarized continuous-wave illumination. We demonstrate, through rigorous three-dimensional numerical simulations, that properly tilting the incident illumination angle can increase or decrease the photoinduced temperature gradients within the nanostructure. We analyze the apex-base photoinduced temperature gradient for different illumination directions, resembling typical illumination schemes utilized in surface or tip-enhanced Raman spectroscopy.

Towards enhanced sodium storage of anatase TiO2via a dual-modification approach of Mo doping combined with AlF3 coating.

Recent studies on anatase TiO2 have demonstrated its capability of performing as an anode material for sodium-ion batteries (SIBs) even though, due to poor conductivity, realistic applications have not yet been foreseen. In order to try to address this issue, herein, we shall introduce a cost effective and facile route based on the co-precipitation method for the synthesis of Mo-doped anatase TiO2 nanoparticles with AlF3 surface coating. The electrochemical measurements demonstrate that the Mo-doped anatase TiO2 nanoparticles deliver an ∼40% enhanced reversible capacity compared to pristine TiO2 (139.8 vs. 100.7 mA h g-1 at 0.1 C after 50 cycles) due to an improved electronic/ionic conductivity. Furthermore, upon AlF3 coating, the overall system can deliver a much higher reversible capacity of 178.9 mA h g-1 (∼80% increase with respect to pristine TiO2) with good cycling stability and excellent rate capabilities of up to 10 C. The experimental results indicate that the AlF3 surface coating could indeed effectively reduce the solid electrolyte interfacial resistance, enhance the electrochemical reactivity at the surface/interface region, and lower the polarization during cycling. The improved performance achieved using a cost-effective fabrication approach makes the dually modified anatase TiO2 a promising anode material for high-performance SIBs.

Textured nanofibrils drive microglial phenotype.

Microglia are highly plastic cells that change their properties in response to their microenvironment. By using immunofluorescence, live-cell imaging, electrophysiological recordings and RNA sequencing, we investigated the regulation of modified bacterial cellulose (mBC) nanofibril substrates on microglial properties. We demonstrate that mBC substrates induce ramified microglia with constantly extending and retracting processes, reminiscent of what is observed in vivo. Patch-clamp recordings show that microglia acquire a more negative resting membrane potential and have increased inward rectifier K+ currents, caused by an upregulation of Kir2.1 channels. Transcriptome analysis shows upregulation of genes involved in the immune response and downregulation of genes linked to cell adhesion and cell motion. Furthermore, Arp2/3 complex activation and integrin-mediated signaling modulate microglial morphology and motility. Our studies demonstrate that mBC nanofibril substrates modulate microglial phenotype, paving the way for a microglia-material interface that may be very valuable for anti-neuroinflammatory drug screening.

Bottom-Gate Approach for All Basic Logic Gates Implementation by a Single-Type IGZO-Based MOS Transistor with Reduced Footprint.

Logic functions are the key backbone in electronic circuits for computing applications. Complementary metal-oxide-semiconductor (CMOS) logic gates, with both n-type and p-type channel transistors, have been to date the dominant building blocks of logic circuitry as they carry obvious advantages over other technologies. Important physical limits are however starting to arise, as the transistor-processing technology has begun to meet scaling-down difficulties. To address this issue, there is the crucial need for a next-generation electronics era based on new concepts and designs. In this respect, a single-type channel multigate MOS transistor (SMG-MOS) is introduced holding the two important aspects of processing adaptability and low static dissipation of CMOS. Furthermore, the SMG-MOS approach strongly reduces the footprint down to 40% or even less area needed for current CMOS logic function in the same processing technology node. Logic NAND, NOT, AND, NOR, and OR gates, which typically require a large number of CMOS transistors, can be realized by a single SMG-MOS transistor. Two functional examples of SMG-MOS are reported here with their analysis based both on simulations and experiments. The results strongly suggest that SMG-MOS can represent a facile approach to scale down complex integrated circuits, enabling design flexibility and production rates ramp-up.

Galvanic Replacement Reaction as a Route to Prepare Nanoporous Aluminum for UV Plasmonics.

There is a growing interest in extending plasmonics applications into the ultraviolet region of the electromagnetic spectrum. Noble metals are commonly used in plasmonic, but their intrinsic optical properties limit their use above 350 nm. Aluminum is probably the most suitable material for UV plasmonics, and in this work we fabricated substrates of nanoporous aluminum starting from an alloy of Al2Mg3. The porous metal is obtained by means of a galvanic replacement reaction. Such nanoporous metal can be exploited to achieve a plasmonic material suitable for enhanced UV Raman spectroscopy and fluorescence. Thanks to the large surface to volume ratio, this material represents a powerful platform for promoting interaction between plasmonic substrates and molecules in the UV.

Facile Synthesis of Highly Graphitized Carbon via Reaction of CaC2 with Sulfur and Its Application for Lithium/Sodium-Ion Batteries.

In the present work, we report, for the first time, a novel one-step approach to prepare highly graphitized carbon (HGC) material by selectively etching calcium from calcium carbide (CaC2) using a sulfur-based thermo-chemical etching technique. Comprehensive analysis using X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and N2 adsorption-desorption isotherms reveals a highly graphitized mesoporous structure for the CaC2-derived carbon with a specific surface area of 159.5 m2 g-1. Microscopic analysis displays micron-scale mesoporous frameworks (4-20 µm) with a distinct layered structure along with agglomerates of highly graphitized nanosheets (about 10 nm in thickness and 1-10 µm lateral size). The as-prepared HGC is investigated for the role of an anode material for lithium- and sodium-ion batteries. We found that HGC exhibits good lithium storage performance in the 0.01-1.5 V range (reversible capacity of 272.4 mA h g-1 at 50 mA g-1 after 100 cycles and 214.2 mA h g-1 at 500 mA g-1 after 500 cycles), whereas, when sodium is considered, we observed a drop in the overall electrochemical performance owing to the high graphitization degree. More importantly, the present study provides a perspective approach to fabricate HGC via a simple, cost-effective, and efficient synthetic route using CaC2 and sulfur as reactants.

Nitrogen-Doped Single-Walled Carbon Nanohorns as a Cost-Effective Carbon Host toward High-Performance Lithium-Sulfur Batteries.

Nitrogen-doped single-walled carbon nanohorns (N-SWCNHs) are porous carbon material characterized by unique horn-shape structures with high surface areas and good conductivity. Moreover, they can be mass-produced (tons/year) using a novel proprietary process technology making them an attractive material for various industrial applications. One of the applications is the encapsulation of sulfur, which turns them as promising conductive host materials for lithium-sulfur batteries. Therefore, we explore for the first time the electrochemical performance of industrially produced N-SWCNHs as a sulfur-encapsulating conductive material. Fabrication of lithium-sulfur cells based on N-SWCNHs with sulfur composite could achieve a remarkable initial gravimetric capacity of 1650 mA h g-1, namely equal to 98.5% of the theoretical capacity (1675 mA h g-1), with an exceptional sulfur content as high as 80% in weight. Using cyclic chronopotentiometry and impedance spectroscopy, we also explored the dissolution mechanism of polysulfides inside the electrolyte.

Gold nanoparticles functionalized by rhodamine B isothiocyanate: A new tool to control plasmonic effects.

Gold nanoparticles with an average diameter of 10 nm, functionalized by the dye molecule rhodamine B isothiocyanate, have been synthesized. The resulting material has been extensively characterized both chemically, to investigate the bonding between the dye molecules and the nanoparticles, and physically, to understand the details of the aggregation induced by interaction between dye molecules on different nanoparticles. The plasmonic response of the system has been further characterized by measurement and theoretical simulation of the static UV-Vis extinction spectra of the aggregates produced following different synthesis procedures. The model parameters used in the simulation gave further useful information on the aggregation and its relationship to the plasmonic response. Finally, we investigated the time dependence of the plasmonic effects of the nanoparticles and fluorescence of the dye molecule using an ultrafast pump-probe optical method. By modulating the quantity of dye molecules on the surface of the nanoparticles it was possible to exert fine control over the plasmonic response of nanoparticles.

Controlling the Heat Dissipation in Temperature-Matched Plasmonic Nanostructures.

Heat dissipation in a plasmonic nanostructure is generally assumed to be ruled only by its own optical response even though also the temperature should be considered for determining the actual energy-to-heat conversion. Indeed, temperature influences the optical response of the nanostructure by affecting its absorption efficiency. Here, we show both theoretically and experimentally how, by properly nanopatterning a metallic surface, it is possible to increase or decrease the light-to-heat conversion rate depending on the temperature of the system. In particular, by borrowing the concept of matching condition from the classical antenna theory, we first analytically demonstrate how the temperature sets a maximum value for the absorption efficiency and how this quantity can be tuned, thus leading to a temperature-controlled optical heat dissipation. In fact, we show how the nonlinear dependence of the absorption on the electron-phonon damping can be maximized at a specific temperature, depending on the system geometry. In this regard, experimental results supported by numerical calculations are presented, showing how geometrically different nanostructures can lead to opposite dependence of the heat dissipation on the temperature, hence suggesting the fascinating possibility of employing plasmonic nanostructures to tailor the light-to-heat conversion rate of the system.

Selective Targeting of Neurons with Inorganic Nanoparticles: Revealing the Crucial Role of Nanoparticle Surface Charge.

Nanoparticles (NPs) are increasingly used in biomedical applications, but the factors that influence their interactions with living cells need to be elucidated. Here, we reveal the role of NP surface charge in determining their neuronal interactions and electrical responses. We discovered that negatively charged NPs administered at low concentration (10 nM) interact with the neuronal membrane and at the synaptic cleft, whereas positively and neutrally charged NPs never localize on neurons. This effect is shape and material independent. The presence of negatively charged NPs on neuronal cell membranes influences the excitability of neurons by causing an increase in the amplitude and frequency of spontaneous postsynaptic currents at the single cell level and an increase of both the spiking activity and synchronous firing at neural network level. The negatively charged NPs exclusively bind to excitable neuronal cells, and never to nonexcitable glial cells. This specific interaction was also confirmed by manipulating the electrophysiological activity of neuronal cells. Indeed, the interaction of negatively charged NPs with neurons is either promoted or hindered by pharmacological suppression or enhancement of the neuronal activity with tetrodotoxin or bicuculline, respectively. We further support our main experimental conclusions by using numerical simulations. This study demonstrates that negatively charged NPs modulate the excitability of neurons, revealing the potential use of NPs for controlling neuron activity.

Dynamics of Strong Coupling between CdSe Quantum Dots and Surface Plasmon Polaritons in Subwavelength Hole Array.

We have investigated the strong coupling interaction between excitons of CdSe quantum dots (QDs) and surface plasmon polaritons (SPPs) of gold nanohole array by steady-state spectroscopic method and transient absorption measurements. Numerical and experimental steady-state measurements demonstrate that the SPP-QD system can indeed undergo strong coupling, characterized by a Rabi splitting up to 220 meV. In particular, it is found that in the transient absorption spectra, under resonant excitation, the 1S transition bleaching band from uncoupled CdSe QDs is completely separated into two distinctive bleaching bands, remarkably fingerprinting the hybrid SPP-QD state. It was also found that the lifetime of these hybrid bands is just slightly shorter than the lifetime of bare CdSe QDs, possibly caused by the phonon bottleneck effect due to the large Rabi splitting. These results could open a new avenue toward the development of novel nanoplasmon devices with strong SPP-QD interaction.

Plasmonic Color-Graded Nanosystems with Achromatic Subwavelength Architectures for Light Filtering and Advanced SERS Detection.

Plasmonic color-graded systems are devices featuring a spatially variable plasmonic response over their surface. They are widely used as nanoscale color filters; their typical size is small enough to allow integration with miniaturized electronic circuits, paving the way to realize novel nanophotonic devices. Currently, most plasmonic color-graded systems are intrinsically discrete because their chromatic response exploits the tailored plasmon resonance of microarchitectures characterized by different size or geometry for each target color. Here, we report the realization of multifunctional plasmon-graded devices where continuously graded chromatic response is achieved by smoothly tuning the composition of the resonator material while simultaneously maintaining an achromatic nanoscale geometry. The result is a new class of versatile materials: we show their application as plasmonic filters with a potential pixel size smaller than half of the exciting wavelength but also as multiplexed surface-enhanced Raman spectroscopy (SERS) substrates. Many more implementations, such as photovoltaic efficiency boosters or color routers, await and will benefit from the low fabrication cost and intrinsic plasmonic flexibility of the presented systems.