Novel pretreatment with hydrogen peroxide enhanced microwave biochar for heavy metals adsorption: Characterization and adsorption performance.

Hydrogen peroxide (HP) was used to pretreat wheat straw (WS) for microwave biochar production at 100-600 W, the physicochemical properties of pretreated WS and biochar products as well as heavy metals adsorption performance were investigated. Results showed that HP enhanced specific surface area (SSA) and pore volume (PV) of WS, and the largest SSA (190.35 m2 g-1) and PV (0.1493 cm3 g-1) of biochar were obtained at microwave powers of 600 W (HPWS600) and 500 W (HPWS500), respectively. HPWS500 showed maximum adsorption capacities, which were 57.56, 190.21, and 65.16 mg g-1 for Cd2+, Pb2+, and Cu2+, respectively. Solution pH values and cation concentrations exhibited significant effects on adsorption capacities of biochar. The pseudo-second-order kinetic and Langmuir isotherm models fitted better for metal adsorption process. The FTIR results suggested that chemisorption mechanisms including precipitation with carbonate and complexation with oxygen-containing functional groups might be predominant adsorption mechanisms. These results suggest that HP pretreatment has excellent potential for biochar production.

Microwave biochar produced with activated carbon catalyst: Characterization and adsorption of heavy metals.

Novel microwave biochar derived from wheat straw (WS) using a range of power levels, with activated carbon catalyst as microwave absorber, was produced, characterized and tested as adsorbent of three heavy metals (Pb2+, Cd2+, and Cu2+). The microwave biochar with the greatest specific surface area (156.09 m2 g-1) and total pore volume (0.0790 cm3 g-1) were produced at 600 W (WS600) and 500 W (WS500) power level, respectively. Maximum adsorption capacities of WS500 to Pb2+, Cd2+ and Cu2+ were 139.44 mg g-1, 52.92 mg g-1, and 31.25 mg g-1, respectively. Optimal pH value for heavy metal removal was at range of 5-6, and Pb2+ showed the strongest affinity in competitive adsorption experiments. The adsorption data were fitted better by pseudo-second-order model and Langmuir isotherm, indicating that adsorption process was mainly explained by monolayer adsorption, and chemical adsorption occupied important role. The predominant adsorption mechanisms of heavy metals on microwave pyrolysis biochar included complexation with oxygen-containing functional groups (i.e., carboxylic acid CO and -OH) and precipitation with carbonate. In addition, reused WS600 maintained 76.17% and 96.07% of their initial adsorption capacity for Cu2+ and Cd2+, respectively. These results suggest that microwave biochar produced with activated carbon catalyst has excellent potential for efficient use in the removal of heavy metals from waste water.

Microwave biochars produced with activated carbon catalyst: Characterization and sorption of volatile organic compounds (VOCs).

A series of microwave biochars derived from wheat straw in the presence of a granulated activated carbon (GAC) catalyst, using a range of microwave conditions, were produced, characterized and tested as sorbents of three benzene series volatile organic compounds (VOCs). The microwave biochar with the greatest specific surface area (SSA), total pore volume (TPV), and micropore volume (312.62 m2 g-1, 0.2218 cm3 g-1, and 0.1380 cm3 g-1, respectively), were produced with 1:3 biomass:GAC catalyst mass ratio, 10 min microwave irradiation time, and at 500 W power level (WB500). Maximum adsorption capacities of WB500 to benzene, toluene and o-xylene were 53.9 mg g-1, 75.8 mg g-1 and 63.0 mg g-1, respectively, and were directly correlated to microwave biochar properties such as SSA, TPV or micropore volume, but were also influenced by VOC properties such as molecular polarity and boiling point. Kinetic modeling suggested that adsorption was governed by both physical partitioning and chemisorption mechanisms. In addition, microwave biochars maintained 79% to 92% of their initial adsorption capacity after ten adsorption/desorption cycles. These results suggest that microwave biochars produced with an GAC catalyst have excellent potential for efficient use in the removal of VOCs from waste gas.

Anaerobic co-digestion of dairy manure and Japanese knotweed (Fallopia japonica) under thermophilic condition: Optimal ratio for biochemical methane production.

Anaerobic co-digestion of animal manure and lignocellulosic biomass is a potent approach for sustainable biomethane production. Co-digestion of dairy manure (DM) and Japanese knotweed (JK), which was collected from a riverbank, was investigated at five different DM-to-JK mixing ratios (100:0, 90:10, 80:20, 60:40, and 0:100; wet weight basis) under thermophilic condition. The results showed that the methane yields obtain from the co-digestion of DM and JK were much higher than that obtained from JK alone (104 ml/gVS), which indicates the synergistic effect and the benefits of co-digesting JK with DM. The highest methane yield (232 ml/gVS) was obtained from the DM-to-JK ratio of 90:10, which was 14.9% and 123.1% higher than that from DM and JK alone, respectively. It also showed the highest synergistic effect (61 ml/gVS). However, further increase in JK ratios led to the decrease in methane yield and synergistic effect. Therefore, applying the co-digestion of DM and JK at a ratio of 90:10 is recommended for biomethane production.

Effect of solid-liquid separation on anaerobic digestion of dairy manure in semi-continuous stirred tank reactors: Process performance and digestate characteristics.

The objective of this study was to investigate the effect of solid-liquid separation on anaerobic digestion of dairy manure in semi-continuous stirred tank reactors. Reactors fed with liquid fraction of dairy manure (screened liquid manure) were kept in water baths at mesophilic (Run 1) and thermophilic (Run 3) temperatures, respectively, while reactors fed with water diluted manure and kept at mesophilic (Run 2) and thermophilic (Run 4) temperatures as control reactors. The performances of reactors were compared in terms of biogas and methane production, and digestate characteristics. The methane yields obtained from screened manure were higher than those from diluted manure at both mesophilic and thermophilic temperatures, while the highest methane yield was 185 L/kg VSadded under thermophilic temperature. Solid-liquid separation also had improved the effect on digestate fertilizer characteristics. Among four digestates from reactors, the highest contents of nutrients, N (4.12 g/kg) and P (2.36 g/kg) were found in Run 3, while the highest content of K (3.42 g/kg) was found in Run 1. These results showed the benefits of solid-liquid separation of dairy manure on process performance and digestate characteristics.

The survival of pathogenic bacteria and plant growth promoting bacteria during mesophilic anaerobic digestion in full-scale biogas plants.

The introduction of biogas plants is a promising way to recycle organic wastes with renewable energy production and reducing greenhouse gas. Application of anaerobic digestate as a fertilizer reduces the consumption of chemical fertilizers. In this study, the survival of pathogenic bacteria and plant growth promoting bacteria (PGPB) in two full-scale biogas plants operated at mesophilic condition were investigated. Feedstock and anaerobic digestate samples were collected from biogas plants and bacteria load in samples were detected using standard dilution plate method. Pathogenic bacteria were reduced to not detected level through mesophilic digestion tank except for Campylobacter. However, it could be reduced by 98.7% through a sterilization tank. Bacillus was detected at 8.00 and 7.81 log10  CFU/g dry matter in anaerobic digestates, and it was also resistant to sterilization tank. Bacillus spp. is considered to be the safe bacteria that hold remarkable abilities for promoting plant growth. The results showed that treatment at biogas plants is effective to reduce pathogenic bacteria in dairy manure, and sterilization could further reduce the sanitary risks of pathogenic bacteria relating to anaerobic digestate application. Anaerobic digestates could also be utilized as bio-fertilizer as the high load of plant growth promoting bacteria.

Potential of anaerobic digestate of dairy manure in suppressing soil-borne plant disease.

Frequent use of pesticides to control soil-borne plant disease leads to environmental pollution and the development of pesticide resistance in phytopathogens. Soil amendment is considered to have the potential of suppressing plant disease because of its biological properties. However, information on anaerobic digestate is limited. In this study, potential of antagonistic activities of anaerobic digestate against phytopathogens were investigated by detecting the amounts of antagonistic bacteria (Bacillus and Pseudomonas) in anaerobic digestates of dairy manure. The results showed that anaerobic digestion increased the total amounts of Bacillus and Pseudomonas in digestate. Bacillus suppressed growth of phytopathogens, while Pseudomonas did not show any antagonistic activities. These results indicated that Bacillus was an effective antagonistic bacterium in digestate against phytopathogens. Furthermore, two selected isolates, B11 (Bacillus subtilis) and B59 (Bacillus licheniformis), were applied in field experiments and showed significant reduction in percent infection of potato late blight (Phytophthora infestans). These results demonstrate the benefits of digestate in suppressing soil-borne plant diseases caused by antagonistic bacteria.