A retrospective study: analysis of the relationship between lactate dehydrogenase and castration-resistant prostate cancer based on restricted cubic spline model.

Background: Different prostate cancer patients take different amounts of time to progress to castration-resistant prostate cancer (CRPC), and this difference in time determines the patient's ultimate survival time. If the time to progression to CRPC can be estimated for each patient, the treatment can be better individualized. Objective: Castration-resistant prostate cancer is a challenge in attacking prostate cancer, the aim of the paper is to analyze the correlation between lactate dehydrogenase (LDH) and CRPC occurrence based on the restricted cubic spline model, and to provide a theoretical basis for LDH as a prognostic biomarker for prostate cancer patients. Methods: We retrospectively analyzed clinical and follow-up data of patients diagnosed with prostate cancer and treated with Androgen Deprivation Therapy (ADT) in our hospital from October 2019 to August 2022. Investigate the correlation between LDH and CRPC by COX regression, restricted cubic spline model and survival analysis. Results: The initial tPSA concentration, prostate volume, LDH and alkaline phosphatase levels in patients with prostate cancer with rapid progression are higher than those in patients with prostate cancer with slow progression. Multivariate COX regression showed that initial tPSA level and LDH level are independent risk factors for prostate cancer. Restricted cubic spline model further showed that LDH level is linearly correlated with the risk of CRPC in prostate cancer patients (total P < 0.05, nonlinear P > 0.05). Conclusion: LDH was associated with the prognosis of prostate cancer and had a dose-response relationship with the risk of CRPC in prostate caner patients.

Semiquantitative Screening of THC Analogues by Silica Gel TLC with an Ag(I) Retention Zone and Chromogenic Smartphone Detection.

With the ever-evolving cannabis industry, low-cost and high-throughput analytical methods for cannabinoids are urgently needed. Normally, (potentially) psychoactive cannabinoids, typically represented by Δ9-tetrahydrocannabinol (Δ9-THC), and nonpsychoactive cannabinoids with therapeutic benefits, typically represented by cannabidiol (CBD), are the target analytes. Structurally, the former (tetrahydrocannabinolic acid (THCA), cannabinol (CBN), and THC) have one olefinic double bond and the latter (cannabidiolic acid (CBDA), cannabigerol (CBG), and CBD) have two, which results in different affinities toward Ag(I) ions. Thus, a silica gel thin-layer chromatography (TLC) plate with the lower third impregnated with Ag(I) ions enabled within minutes a digital chromatographic separation of strongly retained CBD analogues and poorly retained THC analogues. The resolution (Rs) between the closest two spots from the two groups was 4.7, which is almost 8 times higher than the resolution on unmodified TLC. After applying Fast Blue BB as a chromogenic reagent, smartphone-based color analysis enabled semiquantification of the total percentage of THC analogues (with a limit of detection (LOD) of 11 ng for THC, 54 ng for CBN, and 50 ng for THCA when the loaded volume is 1.0 µL). The method was validated by analyzing mixed cannabis extracts and cannabis extracts. The results correlated with those of high-performance liquid chromatography with ultraviolet detection (HPLC-UV) (R2 = 0.97), but the TLC approach had the advantages of multi-minute analysis time, high throughput, low solvent consumption, portability, and ease of interpretation. In a desiccator, Ag(I)-TLC plates can be stored for at least 3 months. Therefore, this method would allow rapid distinction between high and low THC varieties of cannabis, with the potential for on-site applicability.

Effect of interaction between occupational stress and polymorphisms of MTHFR gene and SELE gene on hypertension.

BACKGROUND: Gene-environment interaction is related to the prevalence of hypertension, but the impact of genetic polymorphisms on hypertension may vary due to different geography and population. OBJECTIVE: To explore the impact of the interaction among occupational stress and MTHFR gene and SELE gene polymorphism on the prevalence of hypertension in Xinjiang oil workers. METHODS: A case-control study was conducted on 310 oil workers. In an oilfield base in Karamay City, Xinjiang, 155 hypertensive patients aged 18~60 years old with more than one year of service were selected as the case group, and 155 oil workers without hypertension were selected as the control group according to the 1:1 matching principle (matching conditions: the gender and shift were the same. The age is around 2 years old). The Occupational Stress Scale was used to evaluate the degree of occupational stress, PCR technique was used to detect MTHFR and SELE gene polymorphism, Logistic regression analysis was used to analyze the effects of gene and occupational stress on hypertension, and gene-gene and gene-environment interactions were analyzed by generalized multi-factor dimension reduction method. RESULTS: The G98T polymorphism of SELE gene (χ2 = 6.776, P = 0.034), the C677T (χ2 = 7.130, P = 0.028) and A1298C (χ2 = 12.036, P = 0.002) loci of MTHFR gene and the degree of occupational stress (χ2 = 11.921, P = 0.003) were significantly different between the case group and the control group. The genotypes GT at the G98T polymorphism of the SELE gene (OR = 2.151, 95% CI [1.227-3.375]), and the dominant model (AC/CC vs AA, OR = 1.925, 95% CI [1.613-3.816]); AC and CC at the A1298C polymorphism of the MTHFR gene (OR AC = 1.917, 95% CI [1.064-3.453]; OR CC = 2.233, 95% CI [1.082-4.609]), the additive model (CC vs AA, OR = 2.497, 95% CI [1.277-4.883]) and the dominant model (AC/CC vs AA, OR = 2.012, 95% CI [1.200-3.373]); at the C677T polymorphism of the MTHFR gene CT and TT (OR CT = 1.913, 95% CI [1.085-3.375]; OR TT = 3.117, 95% CI [1.430-6.795]), the additive model (CC vs AA, OR = 1.913, 95% CI [1.085-3.375]) and the dominant model (AC/CC vs AA, OR = 2.012, 95% CI [1.200-3.373]), which could increase hypertension risk (P < 0.05). The gene-gene interaction showed that there was a positive interaction between the A1298C and C677T sites of the MTHFR gene, and the gene-occupational stress interaction showed that there was a positive interaction between the A1298C and C677T sites of the MTHFR gene and the occupational stress. CONCLUSION: The interaction of gene mutation and occupational stress in Xinjiang oil workers maybe increase the risk of hypertension.

[Relationship between sleep disorders and hypertension in oil workers in Karamay City].

OBJECTIVE: To investigate the effect of sleep disorders on hypertension in oil workers and its mediating effect analysis. METHODS: Between June and September 2019, 1420 workers aged 20-60 years(745 males and 675 females; 384 aged 30 years, 563 aged 30-45 years and 473 aged 45 years) from six oilfield bases in Karamay City, Xinjiang Uygur Autonomous Region were surveyed using a two-stage randomized whole-group sampling method. Their current conditions, and the information on gender, age, ethnicity, personal monthly income, education level, job title, smoking, alcohol consumption, height, weight, and shift work of the oil workers were investigated by the basic questionnaire. The Pittsburgh Sleep Index was used to assess the sleep status, and the Logistic regression model was used to analyze the influencing factors of hypertension, and the Process program(version 3.3) was used to conduct the mediating effect model test. RESULTS: (1) The detection rate of sleep disorders was 52.5% and the prevalence of hypertension was 16.3%, with 21.6%(161) higher in women than 10.5%(71) in men(χ~2=31.877, P& lt; 0.001). The prevalence of hypertension increased with increasing age and body mass index(BMI)(χ~2=25.117, P& lt; 0.001), 20.1%(149) in the sleep disorder group were higher than 12.2%(83) in the non-sleep disorder group(χ~2=16.113, P& lt; 0.001). (2) After adjusting for gender, age, ethnicity, education, personal monthly income, smoking, alcohol consumption, shift work and BMI, sleep disorders(OR=1.686, 95% CI 1.232-2.308), gender(OR=1.565, 95% CI 1.035-2.367), age(OR_(30-45)=1.710, 95% CI 1.085-2.697; OR_(& gt; 45)=1.717, 95% CI 1.055-2.797), shift work(OR=2.698, 95% CI 1.889-3.855), BMI(OR_(24.0-27.9)=2.557, 95% CI 1.736-3.765; OR_(≥28.0)=4.001, 95% CI 2.553-6.318) increased the risk of hypertension. (3) The result of stratified analysis showed that with age(OR_(30-45)=1.642, 95% CI 1.019-2.645; OR_(& gt; 45)=1.998, 95% CI 1.223-3.263) and BMI(OR_(24.0-27.9)=1.652, 95% CI 1.079-2.528; OR_(≥28.0)=2.259, 95% CI 1.165-4.381) increased, the risk of hypertension due to sleep disorders increased. Sleep disorders(OR=2.002, 95% CI 1.336-2.936) were also risk factors for hypertension in the shift work group. (4) The result of the mediating effect test showed that there was a mediating effect(P& lt; 0.05) between sleep disorders in age(ß=0.240, Z=2.239), shift work(ß=0.656, Z=3.999), and BMI(ß=0.516, Z=7.258) and hypertension. CONCLUSION: Sleep disorders may be a risk factor affecting hypertension in oil workers in Karamay City, and there were mediating effects between age, shift work, and BMI and hypertension.

Association of sleep duration and sleep quality with hypertension in oil workers in Xinjiang.

OBJECTIVE: The aim of this study is to explore sleep status and hypertension among oil workers in Xinjiang, China. It may provide new ideas and basis for the precise prevention and treatment of hypertension in occupational population. METHODS: Sleep status and hypertension were investigated in 3,040 workers by a multi-stage cluster sampling method in six oil field bases in Karamay City, Xinjiang. The Pittsburgh Sleep Quality Index was used to evaluate the sleep status of workers. Logistic regression was used to analyze the relationship between sleep duration and sleep quality, and hypertension. Stratified analysis was also performed. RESULTS: Our results show: 1. Insufficient sleep duration (OR = 1.51, 95% CI [1.19-1.90]) and poor sleep quality (OR = 1.78, 95% CI [1.33-2.38] were positively associated with hypertension. 2. Stratified analysis indicated insufficient sleep duration was associated with increased risk of hypertension in females (OR = 1.54, 95% CI [1.16-2.04]) than males (OR = 1.49, 95% CI [1.00-2.23]), and the risk of hypertension in the group <30 years old (OR = 9.03, 95% CI [2.32-35.15]) was higher than that in the group of 30-45 years old (OR = 1.59, 95% CI [1.14-2.20]). However, in the group > 45 years old, sleeping > 8 h was associated with increased risk of hypertension (OR = 3.36, 95% CI [1.42-7.91]). Oil workers doing shift work had a higher risk of hypertension (OR = 1.55, 95% CI [1.16-2.07]) to no shift work (OR = 1.48, 95% CI [1.02-2.15]). The risk of hypertension in the group with < 10 years of service (OR = 4.08, 95% CI [1.92-8.83]) was higher than that in the group with length of service of 10-20 years (OR = 2.79, 95% CI [1.59-4.86]). Poor sleep quality was associated with risk for hypertension in females (OR = 1.78, 95% CI [1.26-2.49]), those doing shift work (OR = 1.70, 95% CI [1.17-2.47]), those with length of service of > 20 years (OR = 1.64, 95% CI [1.18-2.27]). The risk of hypertension in the group 30-45 years old is higher than that in the group > 45 years old (OR 30-45 years old = 1.71, 95% CI [1.10-2.66]; OR > 45 years old = 1.60, 95% CI [1.09-2.34]). CONCLUSION: Insufficient sleep duration and poor sleep quality are the potential factors affecting hypertension in Xinjiang oil workers.

Association between hs-CRP Levels and the Outcomes of Patients with Small-Artery Occlusion.

BACKGROUND: High-sensitivity C-reactive protein (hs-CRP) is not only a marker of inflammation but also a prognostic factor for ischemic stroke. The objective of our study was to investigative the association between hs-CRP levels and outcomes of patients with small-artery occlusion (SAO). METHODS: We selected 718 participants diagnosed with SAO (according to Trial of Org 10172 in Acute Stroke Treatment classification) using the stroke registry of the Department of Neurorehabilitation of Tianjin HuanHu Hospital. Hs-CRP values at admission were classified into 3 categories: <0.91 mg/L, 0.91 to <2.77 mg/L, and ≥2.77 mg/L. Patients were divided into two subgroups based on age: the younger subgroup (<75years) and the elder subgroup (≥75 years). Clinical outcomes were evaluated with the modified Rankin scale (mRS) 3 months after the onset of stroke. We examined the relationship between hs-CRP levels at the time of admission and mRS scores using multivariate logistic regression analysis. We also assessed the association between hs-CRP levels and patient outcomes according to age. RESULTS: Among 718 patients with SAO (mean age, 61.7 ± 11.3 years), median hs-CRP was 1.54 mg/L. Although 628 patients had a favorable outcome, and 90 patients had a poor outcome at 3 months after SAO. Compared with the lowest levels of hs-CRP, those highest levels of hs-CRP (hs-CRP > 2.77 mg/L) were at increased risk of poor outcome (adjusted odds ratio, 1.917; 95% CI, 1.050-3.500; P = 0.034), and more than twice the risk of poor outcome among patients in the younger subgroup (adjusted odds ratio, 2.092; 95% CI, 1.079-4.058; P = 0.029). These associations persisted after adjustment for confounding risk factors. However, hs-CRP levels were not significantly associated with outcome among patients in the elder subgroup. CONCLUSIONS: Elevated hs-CRP in patients with SAO is an independent predictor of poor prognosis; however, this association is only present in younger patients (<75 years).

Rh(I)-catalyzed decarbonylative direct C2-olefination of indoles with vinyl carboxylic acids.

A general and efficient Rh(I)-catalyzed decarbonylative direct C2-olefination of indoles with vinyl carboxylic acids has been developed. The reaction exhibits excellent functional group tolerance, regioselectivity and stereoselectivity, giving a broad range of C2-alkenylated indoles in good to excellent yields.