Aesthetic oral rehabilitation of the upper-anterior sector with supra-nano filling resin in a patient with woolly hair syndrome: case report.

BACKGROUND: Woolly Hair Syndrome (WHS) is a rare birth condition that affects the structure of hair in non-black people. The pathogenesis is not yet defined. It is postulated that the hair follicle's desmosomes (specifically desmoplaquine, placoglobin and placofilin-1, which are cell structural proteins that keep the adhesion among close cells) would be altered in this pathology, leading to fragility in the cellular union. It is subdivided into two large groups: the localized or circumscribed variant and the generalized variant. From birth or first months of life, patients with WHS are clinically characterized by the presence of a portion or entire scalp area of very short frizzy hair, usually of a smaller diameter, brittle and lighter color. The most frequent skin manifestations are pilar keratosis and palmo-plantar keratodermia. The diagnosis is based on the clinical findings, and it is facilitated by trichotoscopic examination. However, a definitive diagnosis of WHS requires genetic testing. Oral agenesis, enamel defects (such as hypomineralization), atypical caries, dental inclusion, and malformed pin-shaped teeth may occur. OBJECTIVE: To describe an aesthetic alternative of oral rehabilitation using the mock-up technique in a patient with WHS. CLINICAL CASE: A 5-year-old female patient diagnosed with WHS from the National Institute of Child Health NIHCH: Breña, Lima, who was referred from the Genetic service to the Pediatric Dentistry service in order to screen outbreaks of infection associated with odontogenic origin and dental anomalies. At the ectoscopy, a patient with short capillary length, brittle and curly hair, dry skin, and nail dystrophy was observed. At the intraoral clinical examination, anterior pieces of 52, 51, 61, 62, and enamel hypomineralization were observed in all teeth. The radiographic examination showed agenesis of parts 41, 34, and 45. Integral dental treatment was performed in the operating room under general anesthesia due to the complexity of the case. Pulpectomy in pieces 52, 51, 61, 62, post of composite resin, and rehabilitation with supra-nanow filling resin using the mock-up technique were proposed as alternative treatments. CONCLUSION: The making of supra-nano filling resin-based crowns using the mock-up technique is an alternative treatment for aesthetic oral rehabilitation in deciduous dentition of patients with WHS. The aesthetic treatment was achieved using supra nano-filling resins. After 12 months of dental treatment, a favorable response was observed, improving the chewing, phonation and aesthetics of the patient.

Evolución, secuelas y tratamiento mediante osteotomía de pemberton para la artritis séptica infantil de cadera / Evolution, sequels and treatment by osteotomy of pemberton for childhood septic arthritis of the hip

La osteotomía de Pemberton es utilizada para el tratamiento de la subluxación de la cadera en artritis séptica infantil de la cadera. Las caderas presentaban deformidad de la cabeza femoral asociada a necrosis avascular secundario a la artritis séptica; clasificamos la cadera como tipo Choi I B. secuelas. La edad en la cual fue sometida a cirugía fue a los 8 días de nacido con debridamiento quirúrgico, a los 6 meses mediante capsuloplastia y yeso pelvi pedico y a los 3 años osteotomía de Pemberton con un período de seguimiento promedio de 3 años. Se obtuvieron las reducciones concéntricas. El resultado fue satisfactorio según los criterios de Hunka en el momento del seguimiento más reciente. En la última radiografía de seguimiento la cadera tenía contención exitosa de la cabeza femoral. Mediante el presente caso proponemos osteotomía de Pemberton un procedimiento efectivo para el tratamiento de las secuelas de artritis séptica, y la reconstrucción del techo acetabular por la secuela de la artritis séptica cadera infantil, siempre que se pueda lograr una reducción concéntrica.

The Pemberton osteotomy is used for the treatment of hip subluxation in infantile septic arthritis of the hip. The hips presented deformity of the femoral head associated with avascular necrosis secondary to septic arthritis. We classify the hip as Choi I B. sequelae. The age at which she underwent surgery was 8 days old with surgical debridement, at 6 months with capsuloplasty and pelvic plaster, and at 3 years with Pemberton osteotomy, and the average follow-up period was 3 years. The concentric reductions were obtained. The result was satisfactory according to the Hunka criteria at the time of the most recent follow-up. On the most recent follow-up radiograph the hip had successful femoral head containment. Through the present case, we propose Pemberton osteotomy, an effective procedure for the treatment of the sequelae of septic arthritis, and the reconstruction of the acetabular roof due to the sequelae of septic arthritis in the infantile hip, provided that a concentric reduction can be achieved.

Enhanced Antibacterial Activity through Silver Nanoparticles Deposited onto Carboxylated Graphene Oxide Surface.

The strong bactericidal action of silver nanoparticles (AgNPs) is usually limited by their degree of aggregation. Deposition of AgNPs onto a graphene oxide (GO) surface to generate GO-Ag hybrids has been shown to be an effective method of controlling these aggregation problems. In this sense, a novel carboxylated graphene oxide-silver nanoparticle (GOCOOH-Ag) material has been synthesized, and their antibacterial and biofilm formation inhibitions have been studied. AgNPs decorating the GOCOOH surface achieved an average size of 6.74 ± 0.25 nm, which was smaller than that of AgNPs deposited onto the GO surface. In addition, better distribution of AgNPs was achieved using carboxylated material. It is important to highlight the main role of the carboxylic groups in the nucleation and growth of the AgNPs that decorate the GO-based material surface. In vitro antibacterial activity and antibiofilm-forming action were tested against Gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) and Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Both GO-Ag and GOCOOH-Ag reduced bacterial growth, analyzed by time-kill curves. However, the minimum inhibitory concentration and the minimum bactericidal concentration of GOCOOH-Ag were lower than those of GO-Ag for all strains studied, indicating that GOCOOH-Ag has better antibacterial activity. In addition, both nanomaterials prevent biofilm formation, with a higher reduction of biofilm mass and cell viability in the presence of GOCOOH-Ag. The carboxylation functionalization in GO-based materials can be applied to improve the bactericidal and antibiofilm-forming action of the AgNPs.

Focal adhesion kinase inhibitors in the treatment of solid tumors: Preclinical and clinical evidence.

Focal Adhesion Kinase (FAK) is a 125-kDa cytoplasmic protein kinase that is implicated in several cellular functions. This protein is an attractive molecular target for cancer therapy because a wide variety of studies have demonstrated associations between the activation or elevated expression of FAK and tumor progression, invasion, and drug resistance in malignant tumors. Here, we review the strategies used to inhibit FAK activity in solid tumors. We also include an overview of the preclinical (in vitro and in vivo) and clinical studies on FAK inhibitors.

Highlights from the 7th Oncological Pathology Conference 'Pathological Anatomy in the context of the National Cancer Law: An overview of the Latin American experience', 15, 22 and 23 July 2022, Trujillo, Peru.

The seventh session of the Oncological Pathology Conference (JoPaO) entitled 'Pathological Anatomy in the context of the National Cancer Law: An overview of the Latin American experience', was held virtually on July 15, 22 and 23. Peru was the headquarters for this event, where 17 national and international professors of high academic standing participated. They interacted in a multidisciplinary context through talks with national panellists and the general public. The recent promulgation of the 'National Cancer Law' fosters the development of discussion forums to analyse the national realities and uphold continuous learning about experiences in other Latin American countries with successful cancer programmes, in which pathology holds a principal role. The topics addressed during this JoPaO included the exchange of Latin American cancer management experiences, an emphasis on investments in and the development of strategic plans to improve care, the use of new technologies, laboratory quality control, and the need to advance scientific research.

Aula invertida en el desarrollo de competencias para el control domicilario de mosquitos de importancia para la salud pública / Flipped classroom in the development of skills for home control of mosquitoes of importance to public health

El método de enseñanza-aprendizaje de aula invertida propicia actividades participativas, de esfuerzo y colaboración antes, durante y fuera del salón de clases. Por tal razón, que se propuso la aplicación del aula invertida en el desarrollo de competencias para el control domicilario de mosquitos de importancia para la salud pública en la Universidad Nacional Autónoma de Chota, Camarja, Perú. El estudio fue experimental con una muestra de 72 estudiantes. Para la recolección de datos se aplicó la estrategia didáctica del Modelo Flipped Classroom en dos tiempos (pre y post test). Se usó el software SPSS para medianas y prueba de Wilcoxon considerando un alpha del 5%. Como resultado, en el post-test sobre los factores de riesgo identificados en el domicilio se evidenciaron cambios significativos, donde 72,22% identificaron los recipientes de almacenamiento de agua expuestos, 51,39% Frascos/botellas/envases con agua estancada, 45,83% piletas sin liempieza, 29,17% Llantas/desechos al aire libre con agua estancada, 26,39% Floreros con agua vieja, 20,83% analetas/grietas con agua estancada, 12,50% otros y 8,33% no identifico ningún factor, la comparación de la puntuación promedio en competencias para el control domicilario de mosquitos, antes y después de la aplicación del aula invertida en la Prueba de Wilcoxon mostró una z de -4,81 en la dimesión de identificar, -4,860 proponer y -4862 actuar, todos con p 0,000% (diferencia altamente significativa). El método de aula invertida a través de métodos prácticos interactivos contribuye de manera significativa al desarrollo de competencias para el control domicilario de mosquitos de importancia de salud pública(AU)

The flipped classroom teaching-learning method encourages participatory, effortful, and collaborative activities before, during, and outside the classroom. For this reason, the application of the flipped classroom was proposed in the development of skills for the home control of mosquitoes of importance for public health at the National Autonomous University of Chota, Camarja, Peru. The study was experimental with a sample of 72 students. For data collection, the didactic strategy of the Flipped Classroom Model was applied in two stages (pre and post test). The SPSS software was used for medians and the Wilcoxon test considering an alpha of 5%. As a result, in the post-test on the risk factors identified at home, significant changes were evidenced, where 72.22% identified exposed water storage containers, 51.39% Jars/bottles/containers with stagnant water, 45 83% pools without cleaning, 29.17% tires/outdoor debris with stagnant water, 26.39% vases with old water, 20.83% holes/cracks with stagnant water, 12.50% others and 8.33 % did not identify any factor, the comparison of the average score in skills for home control of mosquitoes, before and after the application of the flipped classroom in the Wilcoxon Test showed a z of -4.81 in the dimension of identifying, - 4,860 propose and -4,862 act, all with p 0.000% (highly significant difference). The flipped classroom method through interactive practical methods contributes significantly to the development of skills for home control of mosquitoes of public health importance(AU)

Rasgos clínicos y epidemiológicos de la viruela símica / Clinical and epidemiological features of monkeypox

La viruela símica (VS) es una enfermedad viral, zoonótica que afecta principalmente a los primates y se transmite a humanos. Dicho virus fue aislado inicialmente de un mono de laboratorio en la República Democrática del Congo en 1970 y a partir de allí, ha circulado en el continente africano, principalmente en República Democrática del Congo, Sudán del Sur, Camerún, República Centroafricana, Liberia, Nigeria, Sierra Leona y República del Congo; durante las últimas cinco décadas. Desde el año 2003 se reportaron casos fuera de África, específicamente en Estados Unidos, Reino Unido, Israel y Singapur; mientras que en mayo de 2022, la OMS; reportó un brote de VS humano en varios países de Europa y América, con más de 38000 casos en 93 países y cinco muertes en África, además de la ausencia de vínculos directos con países endémicos y un cambio en el patrón de transmisión; lo que conllevó al organismo a declarar el brote como emergencia de salud pública de interés internacional. La VS cursa con fiebre, cefalea, mialgias, linfadenopatia y lesiones dérmicas; y aunque la enfermedad no es fácilmente transmisible con tendencia a la curación espontánea; los casos observados actualmente no son típicos, con lesiones similares a las producidas por la viruela humana. La presente revisión tiene el objetivo de actualizar los conocimientos acerca de los rasgos clínicos y epidemiológicos de la VS, para comprender la presentación del brote actual y brindar herramientas que permitan diagnosticar y tratar eficazmente a los pacientes afectados(AU)

Monkeypox (MPX) is a viral, zoonotic disease that mainly affects primates and is transmitted to humans. This virus was initially isolated from a laboratory monkey in the Democratic Republic of the Congo in 1970 and since then has circulated on the African continent, mainly in the Democratic Republic of the Congo, South Sudan, Cameroon, the Central African Republic, Liberia, Nigeria, Sierra Leone, and Republic of the Congo; during the last five decades. Since 2003, cases have been reported outside of Africa, specifically in the United States, the United Kingdom, Israel, and Singapore; while in May 2022, the WHO; reported an outbreak of human MPX in several countries in Europe and America, with more than 38,000 cases in 93 countries and five deaths in Africa, in addition to the absence of direct links with endemic countries and a change in the pattern of transmission; which led the agency to declare the outbreak a public health emergency of international concern. MPX presents with fever, headache, myalgia, lymphadenopathy, and skin lesions; and although the disease is not easily transmissible with a tendency to spontaneous healing; the cases currently observed are not typical, with lesions similar to those produced by smallpox. This review aims to update knowledge about the clinical and epidemiological features of MPX, to understand the presentation of the current outbreak and provide tools that allow effective diagnosis and treatment of affected patients(AU)

In silico and in vitro analysis of FAK/MMP signaling axis inhibition by VO-clioquinol in 2D and 3D human osteosarcoma cancer cells.

The study of novel mechanisms of action of vanadium compounds is critical to elucidating the role and importance of these kinds of compounds as antitumor and antimetastatic agents. This work deals with in silico and in vitro studies of one clioquinol oxidovanadium(iv) complex [VO(clioquinol)2], VO(CQ)2, and its regulation of FAK. In particular, we focus on elucidating the relationship of the FAK inhibition, MMP activity and antimetastatic effects of the complex in human bone cancer cells.

[Oestrus ovis ophthalmomyiasis at an urban area in Southern Peru]. / Oftalmomiasis por Oestrus ovis en un área urbana al sur del Perú.

Ophthalmomyiasis is the ocular infestation by dipterous larvae. It has been described in patients from rural areas, poor hygiene, or contact with animals. However, there are sporadic reports of this infestation in urban areas. We present the case of a young man without risk factors, from an urban area in southern Peru, in whom we found 10 larvae at ocular conjunctiva. The larvae were identified as Oestrus ovis, a fly that usually infests sheep and goats as part of their life cycle. We made a brief review of the case, highlighting the importance of adequately identifying the species that cause myiasis as well as its life cycle.

On the discovery of a potential survivin inhibitor combining computational tools and cytotoxicity studies.

Survivin protein is a metalloprotein member of the inhibitors of apoptosis proteins family, involved in the regulation of programmed cell death. Due to the recent development of antitumor therapies having survivin as molecular target, several strategies to interfere with the expression or function of survivin have emerged. This work describes the discovery of a new potential inhibitor of survivin function using a computer-aided drug design approach. Structure-based virtual screening and molecular dynamic simulations were carried out to select two compounds as possible inhibitors. According to the binding energy, possible ligand localization is in a cavity, close to dimerization interface. Next, cell-based assays were performed on three cell lines: two with tumor phenotype and over-expression of survivin, and another with normal phenotype and low expression of survivin. One of the selected compounds shows a selectively antitumor effect on panel cell lines suggesting that the compound effect could be correlated with the survivin expression.

Oftalmomiasis por Oestrus ovis en un área urbana al sur del Perú / Oestrus ovis ophthalmomyiasis at an urban area in Southern Peru

Resumen La oftalmomiasis es la infestación ocular por larvas de dípteros. Ha sido descrita en pacientes provenientes de áreas rurales, higiene deficiente o por contacto con animales. Sin embargo, existen reportes esporádicos de esta infestación en áreas urbanas. Presentamos el caso de un varón joven, sin factores de riesgo, proveniente de un área urbana al sur del Perú, que presentó 10 larvas en la conjuntiva ocular. Las larvas fueron identificadas como Oestrus ovis, una mosca que suele infestar ovejas y cabras como parte de su ciclo de vida. Realizamos una breve revisión del tema, resaltando la importancia de identificar adecuadamente la especie causante de la miasis, así como su ciclo de vida.

Ophthalmomyiasis is the ocular infestation by dipterous larvae. It has been described in patients from rural areas, poor hygiene, or contact with animals. However, there are sporadic reports of this infestation in urban areas. We present the case of a young man without risk factors, from an urban area in southern Peru, in whom we found 10 larvae at ocular conjunctiva. The larvae were identified as Oestrus ovis, a fly that usually infests sheep and goats as part of their life cycle. We made a brief review of the case, highlighting the importance of adequately identifying the species that cause myiasis as well as its life cycle.

Mobilisation Mechanism of Pathogenicity Islands by Endogenous Phages in Staphylococcus aureus clinical strains.

Staphylococcus aureus pathogenicity islands (SaPIs) are a type of mobile genetic element that play a significant role in the pathogenesis and virulence of this microorganism. SaPIs are integrated in the chromosome under the control of the master repressor Stl, but they can be horizontally transferred at a high frequency due to certain bacteriophages. Thus, a phage protein can bind to the SaPI Stl and induce the SaPI cycle, spreading the SaPI virulence factors to other bacterial populations. We report the dissemination mechanism of SaPIs mediated by endogenous prophages in S. aureus clinical strains. We reveal the induction of SaPIs by a co-resident prophage in seven clinically relevant strains, and we further study this mechanism in MW2, a community-acquired methicillin-resistant S. aureus strain that contains two bacteriophages (ɸSa2mw and ɸSa3mw) and one SaPI (SaPImw2) encoding for three enterotoxins (sec, sel and ear). ɸSa2mw was identified as responsible for SaPImw2 induction, and the specific phage derepressor protein DUF3113 was determined. The Stl-DUF3113 protein interaction was demonstrated, along with the existence of variants of this protein in S. aureus phages with different abilities to induce SaPI. Both Stl and DUF3113 are present in other Staphylococcus species, which indicates that this is a generalised mechanism.

Evaluación del efecto antiespasmódico del extracto hidroalcohólico y del aceite esencial de Artemisia Absinthium L (Ajenjo) in vivo y ex vivo / Evaluation of the antispasmodic effect of the hydroalcoholic extract and the essential oil of Artemisia Absinthium L. (Wormwood) in vivo and ex vivo

Artemisia absinthium L. (Ajenjo), es utilizado en la medicina tradicional en cólicos intestinales. El objetivo de este estudio fue determinar el efecto antiespasmódico del extracto hidroalcohólico al 70% y del aceite esencial de Artemisia absinthium L. El tipo de estudio fue cuasiexperimental. Se evaluó el efecto antiespasmódico por un modelo in vivo para medir la actividad sobre el tránsito intestinal, y un modelo ex vivo en íleon aislado de cobayo sobre las contracciones basales y frente a la acción de la acetilcolina. El análisis fitoquímico del extracto reveló la presencia de alcaloides, glicósidos, taninos, resinas, saponinas, lactonas y azucares reductores. El análisis de GC-MS del aceite esencial muestra como metabolitos mayoritarios: linalol, ß - tuyona, α - tuyona, Oxirane, 2-(Hexyn-1yl)-3 methoxymethylene, myrtenyl acetate. Los resultados en el modelo in vivo donde se evaluó el porcentaje de avance del marcador (carbón activado) para el extracto a las dosis de 600mg/kg con 38.8%, 800mg/Kg con 47.9% y 1000mg/Kg con 46.1% en promedio mucho menores que el grupo control (solución salina fisiológica) 84.7%. Para el aceite esencial 2mg/kg con 39.8%, 1 mg/Kg con 43.5%y 0.5mg/Kg con 54.9% menores que el grupo control (solución tween 1%) 85.5%. Para el fármaco patrón (atropina 1 mg/Kg) con porcentaje de avance de 19.9%, con significancia 0.000 a un nivel de confianza del 95%. Los resultados del efecto sobre las contracciones basales de íleon aislado de cobayo donde se evaluó el porcentaje de inhibición para el extracto a las concentraciones de 900)Jg/mL con 91.3%, 1200)Jg/mL con 90.7% y 1500)Jg/mL con 93.4%; para el aceite esencial a las concentraciones de 0.05mg/mL de 50.6%, 0.1 mg/mL de 76.2% y 0.2mg/mL de 90.1 %, para el fármaco patrón atropina a la concentración 0.03mg/mL con 92.4% y para el grupo control 0.8%, con significancia 0.000 a un nivel de confianza del 95%. Frente a las acción de la acetilcolina se evaluó la máxima amplitud después de la adición de acetilcolina, para el extracto a las concentraciones de 900)Jg/mL de 11.3mV, 1200)Jg/mL de 10.5mV, y 1500)Jg/mL de 1 Omv; para el aceite esencial a las concentraciones de 0.05mg/mL de 9.7mV, 0.1mg/mL de 3.6mV y 0.2mg/ml de 1.6mV; para el fármaco patrón atropina a la concentración 0.03mg/ml de 0.4mV y para el grupo control de 16.2mV. , con significancia 0.000 a un nivel de confianza del 95%. Con los resultados obtenidos se concluye que el aceite esencial y el extracto hidroalcohólico al 70% de de Artemisia absinthium L. "Ajenjo" presentan efecto antiespasmódico in vivo y ex vivo.

Atención al cliente en la Escuela del Ballet Oficial

El trabajo emplea conocimientos de información y atención al cliente así mismo busca el planteamiento de un modelo estratégico de atención al cliente para la Escuela del Ballet Oficial...