RESUMEN
Herein we report the expression profiles of certain immunity genes from Apis mellifera carpatica worker individuals experimentally infected with Pseudomonas entomophila L48 strain. Changes of the relative expression of abaecin, Relish, dorsal, Toll-1, domeless, and Duox genes were monitored by qRT-PCR. Our results were compared with similar ones from Drosophila melanogaster model and suggest that these genes are involved in the anti-infective defense mechanisms. Our study opens investigation avenues for modern prophylactic and therapeutic approaches of infections affecting the honeybees, but also for identifying new orthologous genes involved in the human innate immune response.
Asunto(s)
Abejas/genética , Perfilación de la Expresión Génica , Genes de Insecto , Inmunidad Innata/genética , Proteínas de Insectos/biosíntesis , Pseudomonas/patogenicidad , Enfermedades de los Animales/microbiología , Animales , Abejas/inmunología , Abejas/microbiología , Drosophila melanogaster/genética , Drosophila melanogaster/inmunología , Proteínas de Insectos/genética , Proteínas de Insectos/inmunología , Reacción en Cadena en Tiempo Real de la Polimerasa , Especificidad de la EspecieRESUMEN
In an endeavor to monitor the effects of experimental infections with Pseudomonas aeruginosa on gene expression profiles of the eukaryotic model host, we focused on transient receptor potential-like (trpl) pleiotropic gene of Drosophila melanogaster (the fruit fly). Herein we report qRT-PCR data derived from experimental infections of male fruit flies with different genetic backgrounds by pricking and ingesta procedures, as compared to previous results obtained by microarray technology consecutive to ingesta experiments. Whenever statistically significant, the qRT-PCR results obtained for the whole body and intestine of Oregon wild-type flies infected with P. aeruginosa are in agreement with the microarray ones. Both expression profiling technologies revealed similar values of downregulation, supporting the robustness of trpl as a novel gene model for inquiring hostpathogen interactions. On the other hand, the downregulation of trpl in gammaCop mutant males is not confirmed by qRT-PCR data, suggesting that this mutant background is more sensitive to environmental and experimental conditions.