Effects of 3% binahong ( Anredera cordifolia) leaf extract gel on alveolar bone healing in post-extraction tooth socket wound in Wistar rats ( Rattus norvegicus).

Background: Binahong ( Anredera cordifolia (Ten.) STEENIS) is a widely available herbal plant in Indonesia and has been intensely researched for its healing abilities due to its biological activities, but few have studied its capability in accelerating hard tissue healing in post-extraction tooth sockets. The purpose of this study was to analyse the effects of 3% binahong leaf extract gel on alveolar bone healing in post-extraction sockets in Wistar rats. Methods: In this study, 48 male Wistar rats were randomly allocated to twelve groups. After the extraction of the left mandibular incisor, sockets in Group I to IV were given 3% binahong leaf extract gel, group V to VIII were given a control gel, and group IX to XII were given Gengigel ® for 14 days. The residual socket volume (RSV) and fibroblast proliferation were observed on the 3 rd, 7 th, and 14 th day post-extraction, while the osteoblast and osteocyte proliferation were observed on the 7 th, 14 th, and 28 th day post-extraction. The RSV data were analysed using repeated measure ANOVA and one-way ANOVA, while the histopathological data were analysed using one-way ANOVA. Results: The results showed that the binahong group had the lowest RSV and the highest fibroblast proliferation compared to the other groups on the 7th day (p<0.05) and the highest osteoblast and osteocyte proliferation compared to the other groups on the 14 th day (p<0.05). Conclusion: The experiment showed that 3% binahong leaf extract gel could accelerate wound closure, which was characterized by a greater decrease in the RSV value in comparison to the other treatment groups and could enhance alveolar bone healing by increasing the proliferation of fibroblasts, osteoblasts, and osteocytes.

Hospital laboratory management in dealing with disaster

Objective: This study aims to identify scientific evidence and knowledge currently available related to clinical laboratory management in the face of disasters with the literature review method. Method: This is a literature review study. Various references were collected from online databases, including reports, scientific journals, and books in the last ten years. Articles are screened according to the research objectives. The keywords used are laboratories, hospitals, disasters, Point of Care Testing (POCT). Results: POCT is defined as the tools of a laboratory diagnostic test used in a disaster situation effectively. It can be operated by anyone who has been trained. However, it is quite constrained by temperature, earthquake shocks, and battery life. Storage and reagents supply, such as the strip test, might be prepare before the disaster. Moreover, maintenance and validation of POCT tools in disaster preparation is necessary. Hospitals must prepare POCT to conquer the limitation of electricity sources, human resources, and buildings situation was using a laboratory diagnostic analyzer not possible. The POCT was suitable to be conveyed anywhere both within the hospital and to disaster sites using the vehicle. Conclusion: POCT has the enormous potential as an emergency laboratory examination tool both at the hospital and at the disaster site to grip patient safety. (AU)