Effect of successive recycling and reuse of acid liquor for the synthesis of graphene oxides with higher oxygen-to-carbon ratios.

Graphene has recently drawn exponential attention due to its surprising physicochemical properties and diversified field of applications. Although graphene oxides (GOs), itself is an exclusive material, it is also an intermediate product for the production of reduced graphene oxides (rGOs), graphene and their derivatives, which are other more superficial materials. In this study, GOs with higher oxygen to carbon ratios were synthesized following the Tour method, where the excess feed acid liquor (FAL) of mixed concentrated sulfuric and orthophosphoric acids at a ratio of 90:10 was recovered from the reaction slurries by applying the centrifugation technique. About 80-90 % of the FAL was recycled and reused as feed for the subsequent batches. The changes in the properties of FAL for the five consecutive recycling and reuse were studied. The properties of recycled FALs were investigated by measuring density, moisture content, pH, and ion concentration. The consecutive recycling of FALs tends to increase the moisture content about 0.5% in each recycles. Ion-chromatography (IC) was used to measure the variation in SO42- and PO43- ions in the FALs. The H2SO4 reacts with KMnO4 and crystalized out from the recovered FAL faster than the phosphoric acid. So, sulfuric acid content in the makeover FALs must be greater than primary FAL. The product GOs were characterized using FT-IR, FT-Raman, UVVis, STA, SEM, XPS, Zeta-potential, and particle size analyzers. The variation of the properties of GOs with the changes in the reaction parameters such as temperature and time were investigated and correlated with the product yield. It was observed that the effect of temperature on the reaction rate was found to be negatively and positive with the reaction time. The oxygen-to-carbon atomic ratio from XPS analysis was found 66.7%, which supported the increase in product yields 66.9% in the experimental results. The effect of acid concentration, reaction temperature, and time on the GOs properties were satisfactory, correlated, and easily controllable with the reaction conditions. A higher extent of oxidation and enhanced product yields 65-70% were observed at 60-70 °C and 14-18 h. A mixture of nano- and macro-molecular GOs was obtained, and their compositions were easily controllable and separable by controlling the reaction conditions. A correlation was made among the properties of synthesized GOs, FAL, and recycled FAL and reaction conditions.

Antioxidant activity study and GC-MS profiling of Camellia sinensis Linn.

The antioxidant activity of tea leaves extract is a widely researched topic. Tea leaves, particularly those from the Camellia sinensis Linn plant, have garnered attention due to their potential health benefits attributed to their antioxidant properties. Antioxidants are compounds that can neutralize harmful free radicals in the body, which can damage cells and contribute to various health issues, including cancer, cardiovascular diseases, and aging. In this research, the matured tea leaves which has been considered as agricultural waste in Moulovibazar area of Bangladesh have been investigated as a potential source of antioxidant. Methanol was used as solvent for the extraction of antioxidant. DPPH (1,1-diphenyl-2-picryl hydrazyl) scavenging free radical assay method was used to assess the antioxidant activity of the extracts and ascorbic acid was used as positive control. Gas chromatography with mass spectrometric (GC-MS) analysis method was conducted on this extract to investigate the principal components. The half inhibitory concentration (IC50) values of methanol extract and ascorbic acid were found to be 69.51 µg/mL and 10.70 µg/mL, respectively. Caffeine is the main compound (74.47%) among the eight bioactive compounds was identify and quantified by GC-MS.

Extraction and characterization of a novel cellulosic fiber derived from the bark of Rosa hybrida plant.

The current investigation aims to choose an alternate potential replacement for the nonbiodegradable synthetic fibers used in polymer composites. This goal motivated the thorough characterization of Rosa hybrida bark (RHB) fibers. The research explored fiber characterization such as morphological, mechanical, thermal, and physical properties. The suggested fiber features a percentage of cellulose, hemicellulose molecules, and lignin of 52.99 wt%, 18.49 wt%, and 17.34 wt%, respectively according to chemical composition studies, which improves its mechanical properties. It is suitable for lightweight applications due to its decreased density (1.194 gcm-3). The purpose of the Fourier transform infrared spectroscope was to observe and record how various chemical groups were distributed throughout the surface of the fiber. The presence of 1.41 nm-sized crystalline cellulose and further XRD analysis showed a crystallinity index of 75.48 %. Scanning electron microscope studies revealed that RHB fibers have a rough surface. According to a single fiber tensile test, for gauge length (GL) 40 mm, Young's modulus and tensile strength of RHB fibers were 6.57 GPa and 352.01 MPa, respectively, and for GL 50 mm, 9.02 GPa and 311 MPa, respectively. Furthermore, thermo-gravimetric examination revealed that the isolated fibers were thermally stable up to 290 °C and the kinetic activation energy was found to be 75.32 kJ/mol. The fibers taken from the Rosa hybrida flower plants' bark exhibit qualities similar to those of currently used natural fibers, making them a highly promising replacement for synthetic fibers in polymer matrix composites.

Assessment of heavy metals migrated from food contact plastic packaging: Bangladesh perspective.

Plastic-based food-contact materials are potentially threatening the environment and public health by releasing toxic heavy metals. This study aimed to identify the types of plastic commonly used in Bangladesh as food-contact materials (FCMs) and assess the migration of heavy metals from these FCMs. Plastic types were identified using attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR), and 25 samples were selected based on the category, including Polyethylene Terephthalate (PET), Polyethylene (PE), Polypropylene (PP), Polystyrene (PS), and Polycarbonate (PC). Distilled water, 3% acetic acid, and 15% ethanol were used as food simulants to assess the overall migration of chemicals at 70 °C for 2 h. The concentrations of heavy metals (Pb, Cd, Hg, Cr, and Sb) were analyzed using an Atomic Absorption Spectrophotometer (AAS). Results revealed that the highest overall migration occurred in coffee cups measuring 3.50 ± 0.17 mg/kg (using water simulant) and in yogurt containers with a measurement of 9.17 ± 0.1 mg/kg (using 3% acetic acid). The highest concentrations of Pb, Cd, Hg, Cr, and Sb were found in PP-2 (0.45 ± 0.01 mg/kg), PP-2 (0.36 ± 0.01 mg/kg), PC-5 (0.27 ± 0.01 mg/kg), PET-2 (0.12 ± 0.01 mg/kg), and PET-1 (0.09 ± 0.01 mg/kg), respectively. The concentration of heavy metals migrated from the containers is likely to induce a health risk due to bioaccumulation from long-term ingestion of food packaged in them. The findings of this study added knowledge about harmful heavy metals leached from the FCMs in Bangladesh.

Novel Mutation in the Feline NPC2 Gene in Cats with Niemann-Pick Disease.

Niemann-Pick disease (NP) type C is an autosomal, recessive, and inherited neurovisceral genetic disorder characterized by the accumulation of unesterified cholesterol and glycolipids in cellular lysosomes and late endosomes, with a wide spectrum of clinical phenotypes. This study aimed to determine the molecular genetic alterations in two cases of felines with NP in Japan, a Siamese cat in 1989 and a Japanese domestic (JD) cat in 1998. Sanger sequencing was performed on 25 exons of the feline NPC1 gene and 4 exons of the feline NPC2 gene, using genomic DNA extracted from paraffin-embedded tissue specimens. The sequenced exons were compared with reference sequences retrieved from the GenBank database. The identified mutations and alterations were then analyzed using different prediction algorithms. No pathogenic mutations were found in feline NPC1; however, c.376G>A (p.V126M) was identified as a pathogenic mutation in the NPC2 gene. The Siamese cat was found to be homozygous for this mutation. The JD cat was heterozygous for the same mutation, but no other exonic NPC2 mutation was found. Furthermore, the JD cat had a homozygous splice variant (c.364-4C>T) in the NPC2 gene, which is not known to be associated with this disease. The NPC2:c.376G>A (p.V126M) mutation is the second reported pathogenic mutation in the feline NPC2 gene that may be present in the Japanese cat population.

Abundance, distribution and composition of microplastics in sediment and fish species from an Urban River of Bangladesh.

Microplastics (MPs) are a growing concern due to its ubiquitous presence and exceptional stability. We examined the abundance, dispersion, and attributes of MPs in the sediment and fish species of the Turag River in Bangladesh, which separated the industrial city Gazipur from the Mega-city Dhaka. Collected samples were digested and the chemical structure was identified using ATR-FTIR spectroscopy. The concentration of MPs in the sediment ranged from 10 to 35 particles kg-1, with a mean abundance of 19.2 ± 2.44 particles kg-1and MPs of the fragment type account for 34 % of all. The majority of MPs were discovered in the 3-5 mm size range. MPs concentrations in freshwater fish gut from the Turag River ranged from 0.63 ± 0.18 to 7.0 ± 1.1 microplastics individual-1. Fiber was found to be the most commonly ingested MPs type (43 %), followed by fragments (41 %), film (12 %), and foam (4 %). The most common type of MPs was transparent (34 %) followed by red (28 %), white (20 %), green (9 %) and black (8 %). The results showed that demersal (1.87 ± 0.39 items individual-1) and benthopelagic (1.5 ± 0.26 items individual-1) fish species had more MPs than pelagic fish (0.63 ± 0.18 items individual-1) species. Polypropylene (PP) was found to be the most dominant polymer type in the sediment sample, and PS (39 %) in the fish sample. Polyethylene (PE) was discovered to be 30 % and PP 25 % in sediment and fish samples, respectively. Other commonly detected polymer types include polyamide (PA), polystyrene (PS), cellulose acetate (CA), and polyethylene terephthalate (PET). In different sampling sites along the Turag River, the pollution load index (PLI) of MPs contamination was found to be in the risk level category I. The PLI values reflected the city's anthropogenic influence. The findings of the study could be used to establish a baseline for MPs pollution in Bangladesh's vast freshwater ecosystem.

Macro and microanatomy of some organs of a juvenile male Ganges River dolphin (Platanista gangetica spp. gangetica).

Ganges River dolphins (Platanista gangetica spp. gangetica) are air-breathing, warm-blooded mammals endemic to the Ganges and Karnaphuli rivers of the Indian subcontinent. Nevertheless, very little basic histomorphological research has been conducted on this endangered species. Therefore, this study aimed to describe the morphological features of different organs of P. gangetica. Despite becoming aquatic animals, they showed similarities with terrestrial mammals, such as the pair of lungs and an apical bronchus in the respiratory system, which are pretty standard in ruminants and pigs. However, unlike the terrestrial animal, the tracheobronchial tree was stiffer due to circularly arranged anastomosing plates of the hyaline cartilaginous ring in the trachea, cartilaginous plates in the bronchiole, and thick alveolar septa. The digestive system showed a three-chambered mechanical and glandular stomach similar to the artiodactyles. However, the intestine showed smaller caecum like the monogastric mammal. The urogenital system showed lobulated kidneys, a urinary bladder, a fibroelastic penis with sigmoid flexure, and a long urethral process similar to some terrestrial ruminants. Considering the aquatic environment, all those modifications, unlike terrestrial mammals, are necessary for their adaptation. Thus, this research will broadly help our clinicians and conservationist to take further steps toward disease diagnosis and monitoring of marine health of this endangered species.

Climate change adaptation responses among riparian settlements: A case study from Bangladesh.

As transition areas between aquatic ecosystems and the adjacent terrestrial ones, riparian regions are highly exposed to coastal climate hazards. This article describes how climate change and extreme weather impact vulnerable riparian communities and settlements. The analysis is done by reviewing past research and empirical case studies from riparian rural communities of the impact zone of the Sundarbans in Bangladesh, the world's most extensive mangrove forest. The article discusses the climate-related impacts on households through a Severity Index of Vulnerability and assesses the adaptation responses they may pursue. The principal climate-related vulnerabilities and impacts due to increases in temperature, storm surges, sea flooding, and sea-level rise are seawater intrusion and riverbank erosion. Many households have adopted several autonomous reactive adaptation strategies rather than planned ones, to cope with these impacts. However, government organisations and NGOs provide less than optimal technical and financial support to households for planned and anticipatory adaptive responses. The main barriers to adaptation were the high cost of improved crop varieties, inadequate agricultural extension services, and a lack of knowledge on effective climate adaptation. The restoration of the mangrove ecosystem may increase its resilience and, among other things, make local communities less exposed. The article also presents some adaptation measures proper to reduce the climate-related vulnerability of riparian settlements.

Screening and Carrier Rate of Neuronal Ceroid Lipofuscinosis in Chihuahua Dogs in Japan.

Neuronal ceroid lipofuscinosis (NCL) is a group of rare lethal neurodegenerative lysosomal storage diseases that occur in a range of dog breeds, including Chihuahuas. Recently, a homozygous single base-pair deletion (c.846delT), which causes a frame shift generating a premature stop codon (p.Phe282Leufs13*) in the canine CLN7/MFSD8 gene, has been identified as a causative mutation for NCL in Chihuahuas. The objective of this study was to determine the frequency of the mutant allele and/or carrier rate of NCL in Chihuahuas in Japan using a newly designed real-time PCR assay. Samples of saliva were randomly collected from 1007 Chihuahua puppies during physical examinations prior to the transportation to pet shops. Screening results revealed a carrier rate of 1.29%, indicating a mutant allele frequency (0.00645) that is considered sufficiently high to warrant measures for the control and prevention of this lethal disease. The genotyping assay designed in this study could make a valuable contribution to the control and prevention of NCL.

Impact of lockdown relaxation and implementation of the face-covering policy on mental health: A United Kingdom COVID-19 study.

BACKGROUND: Pandemic mitigation policies, such as lockdown, are known to impact on mental health of individuals. Compulsory face covering under relaxed lockdown restrictions gives assurance of less transmission of airborne infection and has the potential to improve mental health of individuals affected by restrictions. AIM: To examine the association of the lockdown relaxation and the implementation of the face covering policy on the mental health of the general population and sub-groups in the United Kingdom using interrupted time series model. METHODS: Using a web-based cross-sectional survey of 28890 United Kingdom adults carried out during May 1, 2020 to July 31, 2020, changes in mental health status using generalised anxiety disorder (GAD-7), and impact of events scale-revised (IES-R) scales are examined, at the dates of the first lockdown relaxation (July 4, 2020) and the subsequent introduction of face covering (July 24, 2020) in United Kingdom. A sharp regression discontinuity design is used to check discontinuities in mental health outcomes at policy-change dates. RESULTS: Average GAD-7 scores of participants were 5.6, 5.6 and 4.3 during the lockdown period, the lockdown relaxation phase and the phase of compulsory face covering, respectively, with lower scores indicating lower anxiety levels. Corresponding scores for IES-R were 17.3, 16.8 and 13.4, with lower scores indicating less distress. Easing lockdown measures and subsequent introduction of face covering, on average, reduced GAD-7 by 0.513 (95%CI: 0.913-0.112) and 1.148 (95%CI: 1.800-0.496), respectively. Corresponding reductions in IES-R were 2.620 (95%CI: 4.279-0.961) and 3.449 (95%CI: 5.725-1.172). These imply that both lockdown relaxation and compulsory face-covering have a positive association with mental health scores (GAD-7 and IES-R). CONCLUSION: The differential impact of lockdown and relaxation on the mental health of population sub-groups is evident in this study with future implications for policy. Introduction of face covering in public places had a stronger positive association with mental health than lockdown relaxation.

Carrier rate of the c.235G>C mutation in the bovine isoleucyl-tRNA synthetase (IARS) gene of Japanese Black cows at Kagoshima prefecture, Japan, and analysis of the metabolic profiling and reproductive performance of heterozygous cows.

Bovine isoleucyl-tRNA synthetase (IARS) disorder, a major cause of weak calf syndrome, is caused by a homozygous missense (c.235G>C) mutation in the bovine IARS gene of Japanese Black (JB) cattle, which was identified in 2013. However, the extent to which the carrier rate has changed at Kagoshima prefecture, Japan, and whether the carrier status is associated with any clinical or reproductive problems, have yet to be ascertained. In this study, using a real-time polymerase chain reaction-based genotyping assay, we determined the carrier rate in a regional JB cow population at Kagoshima prefecture. Comparative analyses were performed on the metabolic profile test (MPT) results and reproductive performance data obtained for heterozygous carrier and homozygous wild-type cows. In 2009 and 2018, DNA samples were collected from 130 and 462 clinically healthy JB cows, respectively, in Kagoshima prefecture. MPT results and reproductive performance data were evaluated for 62 cows, comprising four heterozygous carriers and 58 wild-type cows. Genotyping revealed that the carrier rate was 6.9% in 2009 and 1.5% in 2018, the difference of which was statistically significant (P<0.005). There were no statistically significant differences between the carrier and wild-type cows with respect to either MPT results or reproductive performance, indicating that the carrier cows have necessary IARS activity to maintain minimal health and reproductive potential.

Psychological impact of COVID-19 pandemic: Protocol and results of first three weeks from an international cross-section survey - focus on health professionals.

BACKGROUND: The psychological impact of COVID-19, resultant measures and future consequences to life will be unveiled in time. AIM: To investigate the psychological impact of COVID-19, resultant restrictions, impact on behaviours and mental wellbeing globally. This early analysis, explores positive and adverse factors and behaviours with focus on healthcare professionals. METHODS: This is a cross-sectional survey, using a questionnaire based on published approaches to understand the psychological impact of COVID-19. The survey will be repeated at 6 months because of rapidly changing situation. RESULTS: We have presented results from first 3 weeks of the survey. Conclusions may change as more individuals take part over time. 7,917 participants completed the survey in the first 3 weeks; 7,271 are from the United Kingdom. 49.7% of the participants are healthcare professionals. There is high representation of female participants. Participants reporting suicidal thoughts is 32%. Healthcare professionals have reported mild depression and anxiety in higher proportions. Increasing age and female gender report higher compliance with government advice on COVID 19 whereas higher education, homeowners, key worker status, high alcohol, drug use and participants with pre-existing suicidal thoughts reported low compliance with government advice. Participants who reported suicidal thoughts pre-COVID are less likely to communicate with friends and family, or engage in coping strategies. CONCLUSIONS: Evidence has shown an adverse psychological impact of previous pandemics on the population, especially wellbeing of healthcare professionals. Research should focus on identifying the need, preparing services and determining the factors that enhance and build resilience. FUNDING: This survey is linked to a MRC global health research program of the Portsmouth-Brawijaya center for Global Health, Population, and Policy, (MR/N006267/1), University of Portsmouth.

Maternal and child health care services' utilization data from the fourth round of district level household survey in India.

In this article, we briefly discuss the data used in the article entitled "How Much Do Conditional Cash Transfers Increase the Utilization of Maternal and Child Health Care Services? New Evidence from Janani Suraksha Yojana in India" (Rahman and Pallikadavath, 2018), which has estimated the effects of demand-side financing program named as Janani Suraksha Yojana (JSY) on the utilization of maternal and child health care services in India, using the fourth round of District Level Household Survey (DLHS-4) surveyed on 76,847 Indian women in 2013-14. This survey contains the detailed information on the women's utilization of maternal and child care services, demographic characteristics, and socio-economic status.

Molecular dynamics of koala retrovirus infection in captive koalas in Japan.

Koala retrovirus (KoRV) is a gammaretrovirus that is becoming endogenous in koalas. Here, we explored the dynamics of KoRV infection in captive koalas in Japan. We isolated peripheral blood mononuclear cells (PBMCs) from 11 koalas, from which we extracted the KoRV genome. We found the prevalence of KoRV provirus in the koalas to be 100%, and the copy number of KoRV proviral DNA in genomic DNA isolated from PBMCs was variable. The KoRV envelope genes from 11 koalas were sequenced and all were found to be KoRV type A. Nucleotide substitution analysis revealed differences in the KoRV env gene sequences of parents and their offspring. Although no viral KoRV RNA was detected in plasma of healthy koalas, a high copy number was found in plasma of a diseased koala (#6). Hematological analysis showed a high white blood cell (WBC) count in the blood of koala #6. Notably, when retested ~ 5 months later, koala #6 was found to be negative for KoRV in plasma, and the WBC count was within the normal range. Therefore, KoRV in the plasma could be a possible indicator of koala health. We also investigated KoRV growth in concanavalin-A-stimulated koala PBMCs by measuring the KoRV provirus copy number in gDNA and the KoRV RNA copy number in cells and culture supernatants by real-time PCR at days 4, 7, and 14 post-culture. We also observed that KoRV isolates were able to infect HEK293T cells. These findings could enhance our understanding of the dynamics of KoRV and its pathogenesis in koalas.

How much do conditional cash transfers increase the utilization of maternal and child health care services? New evidence from Janani Suraksha Yojana in India.

Janani Suraksha Yojana (safe motherhood scheme, or JSY) provides cash incentives to marginal pregnant women in India conditional on having mainly institutional delivery. Using the fourth round of district level household survey (DLHS-4), we have estimated its effects on both intended and unintended outcomes. Our estimates of average treatment effect on the treated (ATT) from propensity score matching are remarkably higher than those found in previous prominent studies using the second and third rounds of the survey (DLHS-2 and DLHS-3). When we apply fuzzy regression discontinuity design exploiting the second birth order, our estimates of local average treatment effect (LATE) are much higher than that of ATT. For example, due to JSY, institutional delivery increases by around 16 percentage points according to ATT estimate but about 23 percentage points according to LATE estimate.

Changes in lectin-binding patterns in the kidneys of canines with immune-complex mediated glomerulonephritis.

We surveyed the kidneys of dogs with immune-complex mediated glomerulonephritis (ICGN) by lectin histochemistry using seven lectins-namely WGA, RCA-I, ConA, PNA, SBA, DBA, and UEA-I. Their binding patterns were compared with those from normal dogs. RCA-I signals became weak in the brush borders of the proximal tubules, whereas DBA signals became positive in Bowman's capsules. Also, varying intensity of the UEA-I signal was noted in the distal tubules, especially in the macula densa. The binding pattern profiles varied among the cases; this diversity in the lectin-binding patterns might be induced as a result of the diverse pathologies seen in canine ICGN.

An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity.

Grammothele lineata, an endophyte isolated in our laboratory from jute (Corchorus olitorius acc. 2015) was found to be a substantial paclitaxel producer. Taxol and its related compounds, produced by this endophyte were extracted by growing the fungus in simple nutrient media (potato dextrose broth, PDB). Taxol was identified and characterized by different analytical techniques (TLC, HPLC, FTIR, LC-ESI-MS/MS) following its extraction by ethyl acetate. In PDB media, this fungus was found to produce 382.2 µgL-1 of taxol which is about 7.6 x103 fold higher than the first reported endophytic fungi, Taxomyces andreanae. The extracted taxol exhibited cytotoxic activity in an in vitro culture of HeLa cancer cell line. The fungal extract also exhibited antifungal and antibacterial activities against different pathogenic strains. This is the first report of a jute endophytic fungus harboring the capacity to produce taxol and also the first reported taxol producing species that belongs to the Basidiomycota phylum, so far unknown to be a taxol producer. These findings suggest that the fungal endophyte, Grammothele lineata can be an excellent source of taxol and can also serve as a potential species for chemical and genetic engineering to enhance further the production of taxol.

Electrodeposition of Polypyrrole and Reduced Graphene Oxide onto Carbon Bundle Fibre as Electrode for Supercapacitor.

A nanocomposite comprising of polypyrrole and reduced graphene oxide was electrodeposited onto a carbon bundle fibre (CBF) through a two-step approach (CBF/PPy-rGO-2). The CBF/PPy-rGO-2 had a highly porous structure compared to a nanocomposite of polypyrrole and reduced graphene oxide that was electrodeposited onto a CBF in a one-step approach (CBF/PPy-rGO), as observed through a field emission scanning electron microscope. An X-ray photoelectron spectroscopic analysis revealed the presence of hydrogen bond between the oxide functional groups of rGO and the amine groups of PPy in PPy-rGO-2 nanocomposite. The fabricated CBF/PPy-rGO-2 nanocomposite material was used as an electrode material in a symmetrical solid-state supercapacitor, and the device yielded a specific capacitance, energy density and power density of 96.16 F g- 1, 13.35 Wh kg- 1 and of 322.85 W kg- 1, respectively. Moreover, the CBF/PPy-rGO-2 showed the capacitance retention of 71% after 500 consecutive charge/discharge cycles at a current density of 1 A g- 1. The existence of a high degree of porosity in CBF/PPy-rGO-2 significantly improved the conductivity and facilitated the ionic penetration. The CBF/PPy-rGO-2-based symmetrical solid-state supercapacitor device demonstrated outstanding pliability because the cyclic voltammetric curves remained the same upon bending at various angles. Carbon bundle fibre modified with porous polypyrrole/reduced graphene oxide nanocomposite for flexible miniature solid-state supercapacitor.

Real-time PCR genotyping assay for canine progressive rod-cone degeneration and mutant allele frequency in Toy Poodles, Chihuahuas and Miniature Dachshunds in Japan.

Canine progressive rod-cone degeneration (PRCD) is a middle- to late-onset, autosomal recessive, inherited retinal disorder caused by a substitution (c.5G>A) in the canine PRCD gene that has been identified in 29 or more purebred dogs. In the present study, a TaqMan probe-based real-time PCR assay was developed and evaluated for rapid genotyping and large-scale screening of the mutation. Furthermore, a genotyping survey was carried out in a population of the three most popular breeds in Japan (Toy Poodles, Chihuahuas and Miniature Dachshunds) to determine the current mutant allele frequency. The assay separated all the genotypes of canine PRCD rapidly, indicating its suitability for large-scale surveys. The results of the survey showed that the mutant allele frequency in Toy Poodles was high enough (approximately 0.09) to allow the establishment of measures for the prevention and control of this disorder in breeding kennels. The mutant allele was detected in Chihuahuas for the first time, but the frequency was lower (approximately 0.02) than that in Toy Poodles. The mutant allele was not detected in Miniature Dachshunds. This assay will allow the selective breeding of dogs from the two most popular breeds (Toy Poodle and Chihuahua) in Japan and effective prevention or control of the disorder.

GM2 Gangliosidosis Variant 0 (Sandhoff Disease) in a Mixed-Breed Dog.

GM2 gangliosidosis variant 0 (Sandhoff disease, SD) is a fatal, progressive, neurodegenerative lysosomal storage disease caused by simultaneous deficiencies of acid ß-hexosaminidases A and B. Canine SD has so far been identified only in two purebreeds. In this article, we present the case of a 10 mo old, male dog of mixed breed that developed progressive neurological signs including ataxia, postural deficit, and visual deficits and finally died at the age of 21 mo. The dog was diagnosed with SD on the basis of the results of biochemical and histopathological analyses. This is the third report of canine SD and the first time it has been identified in a mixed breed.