RESUMEN
Rhipicephalus microplus, known as the hard tick, is a vector for the parasites Babesia spp. and Anaplasma marginale, both of which can cause significant financial losses to the livestock industry. There is currently no effective vaccine for R. microplus tick infestations, despite the identification of numerous prospective tick vaccine candidates. As a result, the current research set out to develop an immunoinformatics-based strategy using existing methods for designing a multi-epitope based vaccination that is not only effective but also safe and capable of eliciting cellular and humoral immune responses. First, R. microplus proteins Bm86, Subolesin, and Bm95 were used to anticipate and link B and T-cell epitopes (HTL and CTL) to one another. Antigenicity testing, allergenicity assessment, and toxicity screening were just a few of the many immunoinformatics techniques used to identify potent epitopes. Multi-epitope vaccine design was chosen based on the antigenic score 0.935 that is promising vaccine candidate. Molecular docking was used to determine the nature of the interaction between TLR2 and the vaccine construct. Finally, molecular dynamic simulation was used to assess the stability and compactness of the resulting vaccination based on docking scores. The developed vaccine was shown to be stable, have immunogenic qualities, be soluble, and to have high expression by in silico cloning. These findings suggest that experimental investigation of the multi-epitope based vaccine designed in the current study will produce achievable vaccine candidates against R. microplus ticks, enabling more effective control of infestations.
Asunto(s)
Proteínas de Artrópodos , Biología Computacional , Epítopos de Linfocito B , Epítopos de Linfocito T , Rhipicephalus , Vacunas , Rhipicephalus/inmunología , Animales , Vacunas/inmunología , Proteínas de Artrópodos/inmunología , Proteínas de Artrópodos/genética , Epítopos de Linfocito T/inmunología , Epítopos de Linfocito B/inmunología , Simulación del Acoplamiento Molecular , Infestaciones por Garrapatas/prevención & control , Infestaciones por Garrapatas/veterinaria , Infestaciones por Garrapatas/inmunología , Simulación de Dinámica Molecular , Epítopos/inmunología , Inmunoinformática , Antígenos , Glicoproteínas de Membrana , Proteínas RecombinantesRESUMEN
@#Rhipicephalus microplus, known as the hard tick, is a vector for the parasites Babesia spp. and Anaplasma marginale, both of which can cause significant financial losses to the livestock industry. There is currently no effective vaccine for R. microplus tick infestations, despite the identification of numerous prospective tick vaccine candidates. As a result, the current research set out to develop an immunoinformatics-based strategy using existing methods for designing a multi-epitope based vaccination that is not only effective but also safe and capable of eliciting cellular and humoral immune responses. First, R. microplus proteins Bm86, Subolesin, and Bm95 were used to anticipate and link B and T-cell epitopes (HTL and CTL) to one another. Antigenicity testing, allergenicity assessment, and toxicity screening were just a few of the many immunoinformatics techniques used to identify potent epitopes. Multi-epitope vaccine design was chosen based on the antigenic score 0.935 that is promising vaccine candidate. Molecular docking was used to determine the nature of the interaction between TLR2 and the vaccine construct. Finally, molecular dynamic simulation was used to assess the stability and compactness of the resulting vaccination based on docking scores. The developed vaccine was shown to be stable, have immunogenic qualities, be soluble, and to have high expression by in silico cloning. These findings suggest that experimental investigation of the multi-epitope based vaccine designed in the current study will produce achievable vaccine candidates against R. microplus ticks, enabling more effective control of infestations.
RESUMEN
Mycoplasma bovis is a highly contagious pathogen that causes clinical or subclinical mastitis. The present study was aimed for the isolation, molecular characterization and antibiogram determination of M. bovis from raw milk samples. Milk samples were collected randomly from lactating cows and buffaloes from different tehsils of district Faisalabad, Pakistan. Samples were inoculated on modified Hayflick medium and biochemical tests were performed for further confirmation of isolated M. bovis. Out of total 400 milk samples, 184 (46%) samples were found positive for culture method. The 16S-rRNA gene polymerase chain reaction was performed for molecular characterization of isolated M. bovis strains. Out of total 400 milk samples, 240 (60%) positive for M. bovis through PCR method were examined. The 16S-rRNA gene PCR positive isolated M. bovis strains were sequenced and results were compared using Maximum-likelihood method and sequenced strains of M. bovis were aligned and analyzed by Clustal W software. Antibiogram of isolated M. bovis strains was analyzed by disc diffusion assay against eight commonly used antibiotics. Tylosin (30µg) and Tilmicosin (15ug) showed inhibition zones of 32.34 ± 1.10 mm and 17.12 ± 0.93 mm respectively against isolated M. bovis which were found sensitive. Isolated M. bovis was found resistant to other commonly used antibiotics. Statistical analysis revealed that p-value was < 0.05 and the odds ratio was >1.0 at 95% CI. This study complemented the lack of epidemiological knowledge of molecular characterization, comparative effectiveness and resistance trends of isolated M. bovis strains against commonly used antibiotics.
Asunto(s)
Bison , Enfermedades de los Bovinos , Mastitis Bovina , Mycoplasma bovis , Femenino , Animales , Bovinos , Leche , Mycoplasma bovis/genética , Pakistán/epidemiología , Prevalencia , Lactancia , Mastitis Bovina/epidemiología , Antibacterianos/farmacología , BúfalosRESUMEN
Abstract Diabetes mellitus (DM) is a non-communicable disease throughout the world in which there is persistently high blood glucose level from the normal range. The diabetes and insulin resistance are mainly responsible for the morbidities and mortalities of humans in the world. This disease is mainly regulated by various enzymes and hormones among which Glycogen synthase kinase-3 (GSK-3) is a principle enzyme and insulin is the key hormone regulating it. The GSK-3, that is the key enzyme is normally showing its actions by various mechanisms that include its phosphorylation, formation of protein complexes, and other cellular distribution and thus it control and directly affects cellular morphology, its growth, mobility and apoptosis of the cell. Disturbances in the action of GSK-3 enzyme may leads to various disease conditions that include insulin resistance leading to diabetes, neurological disease like Alzheimer's disease and cancer. Fluoroquinolones are the most common class of drugs that shows dysglycemic effects via interacting with GSK-3 enzyme. Therefore, it is the need of the day to properly understand functions and mechanisms of GSK-3, especially its role in glucose homeostasis via effects on glycogen synthase.
Resumo O diabetes mellitus (DM) é uma doença não transmissível em todo o mundo, na qual existe nível glicêmico persistentemente alto em relação à normalidade. O diabetes e a resistência à insulina são os principais responsáveis pelas morbidades e mortalidades de humanos no mundo. Essa doença é regulada principalmente por várias enzimas e hormônios, entre os quais a glicogênio sintase quinase-3 (GSK-3) é uma enzima principal e a insulina é o principal hormônio que a regula. A GSK-3, que é a enzima-chave, normalmente mostra suas ações por vários mecanismos que incluem sua fosforilação, formação de complexos de proteínas e outras distribuições celulares e, portanto, controla e afeta diretamente a morfologia celular, seu crescimento, mobilidade e apoptose do célula. Perturbações na ação da enzima GSK-3 podem levar a várias condições de doença que incluem resistência à insulina que leva ao diabetes, doenças neurológicas como a doença de Alzheimer e câncer. As fluoroquinolonas são a classe mais comum de drogas que apresentam efeitos disglicêmicos por meio da interação com a enzima GSK-3. Portanto, é necessário hoje em dia compreender adequadamente as funções e mecanismos da GSK-3, principalmente seu papel na homeostase da glicose via efeitos na glicogênio sintase.
Asunto(s)
Humanos , Resistencia a la Insulina , Diabetes Mellitus , Glucógeno Sintasa Quinasa 3 , Glucosa , HomeostasisRESUMEN
Diabetes mellitus (DM) is a non-communicable disease throughout the world in which there is persistently high blood glucose level from the normal range. The diabetes and insulin resistance are mainly responsible for the morbidities and mortalities of humans in the world. This disease is mainly regulated by various enzymes and hormones among which Glycogen synthase kinase-3 (GSK-3) is a principle enzyme and insulin is the key hormone regulating it. The GSK-3, that is the key enzyme is normally showing its actions by various mechanisms that include its phosphorylation, formation of protein complexes, and other cellular distribution and thus it control and directly affects cellular morphology, its growth, mobility and apoptosis of the cell. Disturbances in the action of GSK-3 enzyme may leads to various disease conditions that include insulin resistance leading to diabetes, neurological disease like Alzheimers disease and cancer. Fluoroquinolones are the most common class of drugs that shows dysglycemic effects via interacting with GSK-3 enzyme. Therefore, it is the need of the day to properly understand functions and mechanisms of GSK-3, especially its role in glucose homeostasis via effects on glycogen synthase.
O diabetes mellitus (DM) é uma doença não transmissível em todo o mundo, na qual existe nível glicêmico persistentemente alto em relação à normalidade. O diabetes e a resistência à insulina são os principais responsáveis pelas morbidades e mortalidades de humanos no mundo. Essa doença é regulada principalmente por várias enzimas e hormônios, entre os quais a glicogênio sintase quinase-3 (GSK-3) é uma enzima principal e a insulina é o principal hormônio que a regula. A GSK-3, que é a enzima-chave, normalmente mostra suas ações por vários mecanismos que incluem sua fosforilação, formação de complexos de proteínas e outras distribuições celulares e, portanto, controla e afeta diretamente a morfologia celular, seu crescimento, mobilidade e apoptose do célula. Perturbações na ação da enzima GSK-3 podem levar a várias condições de doença que incluem resistência à insulina que leva ao diabetes, doenças neurológicas como a doença de Alzheimer e câncer. As fluoroquinolonas são a classe mais comum de drogas que apresentam efeitos disglicêmicos por meio da interação com a enzima GSK-3. Portanto, é necessário hoje em dia compreender adequadamente as funções e mecanismos da GSK-3, principalmente seu papel na homeostase da glicose via efeitos na glicogênio sintase.
Asunto(s)
Humanos , Diabetes Mellitus/enzimología , Fluoroquinolonas/análisis , /análisisRESUMEN
Abstract Diabetes mellitus (DM) is a non-communicable disease throughout the world in which there is persistently high blood glucose level from the normal range. The diabetes and insulin resistance are mainly responsible for the morbidities and mortalities of humans in the world. This disease is mainly regulated by various enzymes and hormones among which Glycogen synthase kinase-3 (GSK-3) is a principle enzyme and insulin is the key hormone regulating it. The GSK-3, that is the key enzyme is normally showing its actions by various mechanisms that include its phosphorylation, formation of protein complexes, and other cellular distribution and thus it control and directly affects cellular morphology, its growth, mobility and apoptosis of the cell. Disturbances in the action of GSK-3 enzyme may leads to various disease conditions that include insulin resistance leading to diabetes, neurological disease like Alzheimers disease and cancer. Fluoroquinolones are the most common class of drugs that shows dysglycemic effects via interacting with GSK-3 enzyme. Therefore, it is the need of the day to properly understand functions and mechanisms of GSK-3, especially its role in glucose homeostasis via effects on glycogen synthase.
Resumo O diabetes mellitus (DM) é uma doença não transmissível em todo o mundo, na qual existe nível glicêmico persistentemente alto em relação à normalidade. O diabetes e a resistência à insulina são os principais responsáveis pelas morbidades e mortalidades de humanos no mundo. Essa doença é regulada principalmente por várias enzimas e hormônios, entre os quais a glicogênio sintase quinase-3 (GSK-3) é uma enzima principal e a insulina é o principal hormônio que a regula. A GSK-3, que é a enzima-chave, normalmente mostra suas ações por vários mecanismos que incluem sua fosforilação, formação de complexos de proteínas e outras distribuições celulares e, portanto, controla e afeta diretamente a morfologia celular, seu crescimento, mobilidade e apoptose do célula. Perturbações na ação da enzima GSK-3 podem levar a várias condições de doença que incluem resistência à insulina que leva ao diabetes, doenças neurológicas como a doença de Alzheimer e câncer. As fluoroquinolonas são a classe mais comum de drogas que apresentam efeitos disglicêmicos por meio da interação com a enzima GSK-3. Portanto, é necessário hoje em dia compreender adequadamente as funções e mecanismos da GSK-3, principalmente seu papel na homeostase da glicose via efeitos na glicogênio sintase.
RESUMEN
Diabetes mellitus (DM) is a non-communicable disease throughout the world in which there is persistently high blood glucose level from the normal range. The diabetes and insulin resistance are mainly responsible for the morbidities and mortalities of humans in the world. This disease is mainly regulated by various enzymes and hormones among which Glycogen synthase kinase-3 (GSK-3) is a principle enzyme and insulin is the key hormone regulating it. The GSK-3, that is the key enzyme is normally showing its actions by various mechanisms that include its phosphorylation, formation of protein complexes, and other cellular distribution and thus it control and directly affects cellular morphology, its growth, mobility and apoptosis of the cell. Disturbances in the action of GSK-3 enzyme may leads to various disease conditions that include insulin resistance leading to diabetes, neurological disease like Alzheimer's disease and cancer. Fluoroquinolones are the most common class of drugs that shows dysglycemic effects via interacting with GSK-3 enzyme. Therefore, it is the need of the day to properly understand functions and mechanisms of GSK-3, especially its role in glucose homeostasis via effects on glycogen synthase.
Asunto(s)
Diabetes Mellitus , Resistencia a la Insulina , Glucosa , Glucógeno Sintasa Quinasa 3 , Homeostasis , HumanosRESUMEN
Viral diseases have caused devastating effect on poultry industry leading to significant losses in economy of world. In the presented study, the ability of Newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and avian influenza virus (AIV) to grow in two cell lines was evaluated. Both chicken embryo fibroblast (CEF) and DF-1 cells were used and cytopathic effects (CPE) produced by these viruses were observed. The titer of virus in terms of TCID was determined after 24h up to four days for each virus. The same type of CPE was observed for all virus- es used in the study in both DF-1 and CEF cells. IBDV showed CPE causing rounding of cells while NDV caused formation of multicellular large nuclei, cell fusion and rounding of cells. Giant cells with inclusions and aggregation of cells with intact monolayer was observed for AIV. In growth kinetic study, higher titer of IBDV and NDV was observed in CEF cells than DF-1 cells while for AIV, DF-1 cells showed higher titer than CEF cells. These results would be useful for furthers comparative studies on growth of different cell lines of various viruses to find a suitability for vaccine production.
Asunto(s)
Fibroblastos/virología , Virus de la Enfermedad Infecciosa de la Bolsa/fisiología , Virus de la Influenza A/fisiología , Virus de la Enfermedad de Newcastle/fisiología , Replicación Viral/fisiología , Animales , Línea Celular , Embrión de Pollo , Factores de Tiempo , Cultivo de VirusRESUMEN
AIM: To investigate the occurrence of ESBL and colistin-resistant E. coli and its genotypic characterization and identification of virulence determinants in cases of bovine mastitis in three provinces of China. MATERIALS AND METHODS: Five hundred and thirty-five milk samples presenting mastitis symptoms were screened for the presence of ESBL, colistin-resistant and different virulence genes. Susceptibility testing was identified by the micro-dilution method. Escherichia coli isolates were used to detect ESBL genes (blaCTX-M , blaSHV and blaTEM ) and colistin-resistant genes mcr-(1-9). Multiplex PCR approach was used for the detection of major blaCTX-M groups, different phylogroups and virulence genes. The clonal relationship was then evaluated with MLST, MLSA and PFGE. RESULTS: Multi-drug resistance (MDR) was found in more than 85% of ESBL and colistin-resistant E. coli isolates. Genotypic characterization showed a dominance of the blaCTX-M-1 group, and the most prevalent alleles observed were blaCTX-M-28 (38·37%), blaCTX-M-14 (17·44%), blaCTX-M-66 (13·95%) and blaCTX-M-55 (10·46%). The targeted virulence genes were detected in 97·89% of isolates. Sequence types ST58 and ST410 were the most predominant (2/20 = 20%). The majority of the E. coli isolates carrying ESBL and mcr-1 were clonally unrelated. CONCLUSION: High level of association was observed between ESBL-producing and COL-resistance in E. coli of bovine mastitis. SIGNIFICANCE AND IMPACT OF THE STUDY: To the best of our knowledge, this is the first report which shows the genetic diversity of ESBL and mcr-1, and various virulent features of E. coli strains isolated from bovine clinical mastitis in three different provinces of China. The major carriers of the blaCTX-M-1 and blaCTX-M-9 were blaCTX-M-28 and blaCTX-M-14 alleles respectively. The association of ESBL-producing E. coli with mcr-1 is of particular concern.
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Infecciones por Escherichia coli , Proteínas de Escherichia coli , Escherichia coli , Mastitis Bovina , Factores de Virulencia , Animales , Antibacterianos/farmacología , Bovinos , China , Escherichia coli/genética , Escherichia coli/patogenicidad , Infecciones por Escherichia coli/veterinaria , Proteínas de Escherichia coli/genética , Femenino , Mastitis Bovina/microbiología , Leche/microbiología , Tipificación de Secuencias Multilocus , Plásmidos , Factores de Virulencia/genética , beta-Lactamasas/genéticaRESUMEN
This narrative review on the use of biophotonics therapies for management of oral diseases is written as a tribute to Prof. Crispian Scully. His seminal contributions to the field are highlighted by the detailed, comprehensive description of clinical presentations of oral diseases. This has enabled a more thorough, fundamental understanding of many of these pathologies by research from his group as well as inspired mechanistic investigations in many groups globally. In the same vein, a major emphasis of this narrative review is to focus on the evidence from human case reports rather than in vitro or in vivo animal studies that showcases the growing and broad impact of biophotonics therapies. The similarities and differences between two distinct forms of low-dose biophotonics treatments namely photodynamic therapy and photobiomodulation therapy are discussed. As evident in this review, a majority of these reports provide promising evidence for their clinical efficacy. However, a lack of adequate technical details, precise biological rationale, and limited outcome measures limits the current utility of these treatments. Future investigations should attempt to address these shortcomings and develop better designed, rigorous, controlled studies to fully harness the tremendous potential of low-dose biophotonics therapies.
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Infecciones Bacterianas/tratamiento farmacológico , Terapia por Luz de Baja Intensidad , Enfermedades de la Boca/tratamiento farmacológico , Enfermedades de la Boca/radioterapia , Fotoquimioterapia , Fotones/uso terapéutico , Infecciones Bacterianas/prevención & control , Biopelículas , Endodoncia , Humanos , Terapia por Láser , Aprendizaje , Óptica y Fotónica , FenotipoRESUMEN
There is growing interest in ternary oxide surfaces due to their role in areas ranging from substrates for low power electronics to heterogeneous catalysis. Descriptions of these surfaces to date focus on low-temperature explanations where enthalpy dominates, and less on the implications of configurational entropy at high temperatures. We report here the structure of three members of the n×n (2≤n≤4) reconstructions of the strontium titanate (111) surface using a combination of transmission electron diffraction, density functional theory modeling, and scanning tunneling microscopy. The surfaces contain a mixture of the tetrahedral TiO_{4} units found on the (110) surface sitting on top of octahedral TiO_{5}[] (where [] is a vacant octahedral site), and TiO_{6} units in the second layer that are similar to those found on the (001) surface. We find clear evidence of a transition from the ordered enthalpy-dominated 3×3 and 4×4 structures to a configurational entropy-dominated 2×2 structure that is formed at higher temperatures. This changes many aspects of how oxide surfaces should be considered, with significant implications for oxide growth.
RESUMEN
1. Domestic fowl and free-living birds were examined for the presence or absence of antibodies against hydropericardium syndrome (HPS) using an indirect haemagglutination assay. 2. Two-hundred and eighty serum samples of commercial (45 broilers, 20 adult layers and 15 Fayoumi fowl) and wild birds, including 65 peafowl, 45 pigeons, 10 crows, 30 house sparrows, 10 doves, 15 ducks, 10 parrots and 15 guinea fowl, were collected and examined. 3. The percentage of HPS-positive serum samples was 80% in house crows, 78% in pigeons, 7% in house sparrows and 6% in peafowl. 4. The sera obtained from parrots, doves, ducks and guinea fowl were all negative. 5. This study suggests that crows and pigeons could be carriers of the HPS agent.
Asunto(s)
Infecciones por Adenoviridae/veterinaria , Anticuerpos Antivirales/sangre , Enfermedades de las Aves/inmunología , Aves/inmunología , Cardiopatías/virología , Pericardio , Adenoviridae/aislamiento & purificación , Infecciones por Adenoviridae/inmunología , Animales , Pollos/inmunología , Columbidae/inmunología , Cuervos/inmunología , Patos/inmunología , Cardiopatías/inmunología , Hígado/virología , Loros/inmunología , Aves de Corral/inmunología , Gorriones/inmunologíaRESUMEN
This paper presents the finding of a study undertaken to determine the naturally occurring radionuclides present in commonly used building materials for dwellings and workplaces in four districts of the Punjab Province, Pakistan. The concentrations of (226)Ra, (232)Th and (40)K were measured by using gamma-ray spectroscopy. A total of 80 samples of building materials were collected from various manufacturers and suppliers of the studied area. The specific activities of (226)Ra, (232)Th and (40)K were measured in building samples, and results ranged from minimum values of 9 ± 1, 9 ± 2 and 27 ± 8 Bq kg(-1) to maximum values of 106 ± 5, 133 ± 5 and 914 ± 21 Bq kg(-1) with mean values of 42 ± 3, 48 ± 3 and 376 ± 16 Bq kg(-1), respectively. From the measured activity concentrations, equivalent radium (Ra(eq)), terrestrial absorbed dose rate, annual effective dose as well as external and internal hazard indices were calculated and found to range from 52 ± 7 to 274 ± 15 Bq kg(-1), 23 ± 3 to 130 6 nGy h(-1), 0.15 ± 0.02 to 0.80 ± 0.03 mSv, 0.14 ± 0.02 to 0.75 ± 0.04 and 0.2 ± 0.02 to 0.98 ± 0.05, respectively. These results were comparable to the results of similar studies undertaken locally and in other countries. The samples considered were safe for use in construction of dwellings in the study area and do not pose any significant source of radiation hazard.
Asunto(s)
Materiales de Construcción/análisis , Radioisótopos de Potasio/análisis , Radioisótopos/análisis , Radio (Elemento)/análisis , Torio/análisis , Geografía , Vivienda , Modelos Estadísticos , Pakistán , Monitoreo de Radiación/métodos , Seguridad , Espectrometría gamma/métodosRESUMEN
Indoor air quality has acquired considerable importance in recent years. Tighter buildings with poorer ventilation systems have led towards higher levels of indoor air pollution. Radon is considered to be most significant perilous gas among the various air contaminants found in the residential environment. To determine the risk posed by residential radon exposure, a survey was carried out in the Hattian district of the state of Azad Jammu and Kashmir, Pakistan. In this context, 160 houses were carefully selected for the installation of CR-39-based National Radiological Protection Board-type detectors installation. After exposing the CR-39 detectors for a period of 90 d, they were etched in 6 M chemical solution of sodium hydroxide at a temperature of 80°C for a period of 16 h. The detectors were read under an optical microscope and observed track densities were converted into the indoor radon concentration using a calibration factor of 2.7 tracks cm(-2) h(-1) per kBqm(-3). For the current study, observed radon concentrations ranged from 35 to 175 Bqm(-3), whereas the mean annual effective radon doses received by the inhabitants of the area ranged from 0.88 ± 0.12 to 4.41 ± 0.20 mSv with an average value of 2.62 ± 0.12 mSv. These reported values are less than the limits (standards) recommended by the different world organisations.
Asunto(s)
Contaminantes Radiactivos del Aire/análisis , Contaminación del Aire Interior/análisis , Monitoreo de Radiación/métodos , Radiometría/métodos , Radón/análisis , Calibración , Monitoreo del Ambiente/métodos , Vivienda , Humanos , Neoplasias Pulmonares/etiología , Neoplasias Pulmonares/prevención & control , Neoplasias Inducidas por Radiación/prevención & control , Pakistán , Dosis de Radiación , Radiometría/instrumentación , Riesgo , Temperatura , Factores de TiempoRESUMEN
Several epidemiological studies conducted on thousands of underground miners suggest that long- term exposure to high radon concentration can increase the risk of lung cancer. Keeping in view the importance of the subject, numerous studies throughout the world have been carried out to measure indoor radon concentration and its resulting doses at occupational and non-occupational sites. The purpose of the current study was to measure indoor radon concentration and its resulting doses received by the students of Azad Kashmir government schools. For this purpose, CR-39 radon detectors were installed in 80 carefully selected schools. The detectors were placed at a height of 3-5 ft. (depending upon average height of students in particular class) from the ground. After exposure of 90 d detectors were etched for 9 h in 6 M NaOH at 70°C and the observed track densities were related to radon concentrations. The measured indoor radon concentration ranged from 22 ± 9 to 228 ± 3 Bq m(-3) with a mean value of 78 ± 5 Bq m(-3). Based on the measured indoor radon data, the annual effective doses were found to vary from 0.55 ± 0.04 to 0.71 ± 0.03 mSv y(-1). The overall mean effective dose for the studied area was found to be 0.63 ± 0.04 mSv y(-1). Reported values for radon concentrations and corresponding doses are lower than ICRP recommended limits for workplaces.
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Contaminantes Radiactivos del Aire/análisis , Contaminación del Aire Interior/análisis , Monitoreo de Radiación , Radón/efectos adversos , Radón/análisis , Instituciones Académicas/estadística & datos numéricos , Estudiantes/estadística & datos numéricos , Niño , Preescolar , Femenino , Humanos , Masculino , Pakistán , Dosis de RadiaciónRESUMEN
The objective of this study was to determine the distribution of somatotrophs and lactotrophs and conduct a morphometrical analysis of immunoreactive somatotrophs and lactotrophs in the pituitary glands of White Leghorn Hens (Gallus domesticus) during the period of induced moult. We divided the periods of induced moulting into three phases viz. 7, 14 and 21 days. The labeled alkaline-phsphatase method with anti-GH (growth hormone) and anti-PRL (prolactin) as a primary antibody was used to detect somatotrophs and lactotrophs, in the midsagital sections of chicken adenohypophysis. Immunohistochemistry showed that somatotrophs are not only confined to the cephalo-caudal axis but can also be found in the caudal lobe; while lactotrophs were distributed in both lobes of the anterior pituitary gland at all stages of moulting (7, 14 and 21 days). Lactotrophs were of different shapes but somatotrophs were oval to round in morphology. At the given stages of induced moulting, some hypertrophied lactotrophs were also present after 7 days of induced moult in the anterior pituitary gland. However, there were moulting-related changes: from 7 to 21 days of induced moulting the immunoreactive-PRL cell population decreased, while the mean lactotroph size was more than that of somatotrophs. Basic quantitative and morphological information relating to somatotrophs and lactotrophs during the period of induced moult in laying hens is reported here and the changes brought about by induced moulting are restricted to PRL positive cells rather than GH positive cells.
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Pollos/fisiología , Lactotrofos/citología , Muda/fisiología , Somatotrofos/citología , Animales , Diferenciación Celular , Corticosterona/sangre , Hormona del Crecimiento/metabolismo , Inmunohistoquímica , Lactotrofos/metabolismo , Somatotrofos/metabolismoRESUMEN
The present study deals with measurement of indoor radon concentrations in dwellings of the district Poonch of the state of Azad Jammu and Kashmir, Pakistan. In this context, CR-39-based box-type radon detectors were installed in drawing rooms and bedrooms of 80 selected houses and were exposed to indoor radon for 3 months. After exposure, the CR-39 detectors were etched for 9 h in 6 mol NaOH at 70 degrees C and the observed track densities were related to radon concentrations. Measured indoor radon concentrations in the studied area ranged from 27 +/- 6 to 169 +/- 4, 29 +/- 6 to 196 +/- 4 and 31 +/- 5 to 142 +/- 2 Bq m(-3) in the drawing rooms and 74 +/- 5 to 172 +/- 3, 32 +/- 6 to 191 +/- 4 and 27 +/- 5 to 155 +/- 2 Bq m(-3) in bedrooms of the Abbaspur, Hajira and Rawalakot regions of the district Poonch, respectively; whereas weighted average radon concentration ranged from 93 +/- 6 to 159 +/- 4, 33 +/- 5 to 118 +/- 3 and 31 +/- 6 to 155 +/- 5 Bq m(-3) in the dwellings of Abbaspur, Hajira and Rawalakot, respectively. Estimated doses due to the indoor radon ranged from 2.35 +/- 0.15 to 4.00 +/- 0.10, 0.83 +/- 0.08 to 2.98 +/- 0.08 and 0.78 +/- 0.15 to 3.91 +/- 0.13 mSv y(-1) for Abbaspur, Rawalakot and Hajira, respectively. Comparing the current indoor radon results with those of the Health Protection Agency UK and US EPA (i.e. 200 and 148 Bq m(-3)) limits, majority of the houses surveyed in the present study are within the safe limits.
Asunto(s)
Contaminación del Aire Interior/análisis , Contaminación Radiactiva del Aire/análisis , Monitoreo de Radiación , Radón/análisis , Vivienda , Humanos , Pakistán , Dosis de RadiaciónRESUMEN
It is a well known fact that natural radionuclides are the major contributors towards the gamma ray exposure received by the general public. In this context, soil samples were collected from the Islamabad capital territory and activities due to (226)Ra, (232)Th, (40)K and (137)Cs were measured using gamma ray spectrometry: activities ranged from 41.5-106.2 Bq kg(-1), 4.0-193.8 Bq kg(-1), 325.3-657.4 Bq kg(-1) and 1.6-9.45 Bq kg(-1), respectively. From the measured activities, the external hazard index, internal hazard index, absorbed dose rate and mean annual effective dose were calculated. External and internal hazard indices were calculated as 0.76 and 0.95, respectively. The mean absorbed dose rate was found to be 130.97 nGy h(-1). The mean effective dose was estimated as 0.16 mSv y(-1), which is less than the maximum permissible dose of 1 mSv y(-1).
Asunto(s)
Carga Corporal (Radioterapia) , Exposición a Riesgos Ambientales/análisis , Monitoreo de Radiación/métodos , Radioisótopos/análisis , Contaminantes Radiactivos del Suelo/análisis , Ciudades , Recolección de Datos , Dosis de Radiación , Población UrbanaRESUMEN
Studies concerning measurements of indoor radon levels were carried out in 60 schools in the Rawalpindi region of Pakistan. In each school, six CR-39 based NRPB type radon detectors were installed and exposed to the indoor radon in two cycles (each of six months' duration). After exposure, the detectors were removed, etched in 6 M NaOH for 16 h at 80 degrees C, and the tracks were counted under an optical microscope. The measured track densities were then related to radon concentrations, from which the radiation doses were calculated. The observed radon concentrations varied from 15 to 140 Bq m(-3), with an average activity concentration of 42.75 +/- 9.28 Bq m(-3). The mean annual radon effective dose equivalent was found to be 0.40 +/- 0.09 mSv using an occupancy factor of 8 h day(-1). Our results show that the indoor radon concentrations in the schools surveyed are within the permissible limits.