RESUMEN
The current study evaluates antibiotic susceptibility and Extended Spectrum ß-Lactamase (ESBL) production of 557 Escherichia coli isolates obtained from clean catch midstream urine samples using VITEK 2 compact automated microbial identification system. Different classes of drugs were used to determine the Minimum inhibitory concentration (MIC). In our study, 50.45% of isolates were ESBL producers. There is a higher incidence of UTI in females (77.4%) than in males (22.6%). The isolates reveal a high percentage of resistance to antibiotics like nalidixic acid (89.59%), ampicillin (75.76%), ticarcillin (73.43%), cefalotin (67.68%), cefixime (65.17%), ciprofloxacin (58.35%) and ceftriaxone (56.37%). An increased susceptibility pattern was observed for the isolates against drug classes like fosfomycin (98.03%) and nitrofurantoin (91.02%). Among the isolates, 395 (70.91%) were classified as Multidrug-resistant organisms based on the resistance pattern observed against three or more classes of antibiotics. One of the isolates resistant to fluoroquinolones, penicillins, penicillins along with ß-lactamase inhibitor, aminoglycosides, third-generation cephalosporins and carbapenems was subjected to Whole genome sequencing (WGS). WGS data revealed the isolate to be a high-risk clone ST410, which contains antimicrobial-resistance genes (blaTEM-1B, blaCTX-M-15, blaNDM-5, aac(3)-IId, armA, gyrA(p.S83L), gyrA(p.D87N)) conferring resistance to ß-lactam, cephalosporins, carbapenem, aminoglycoside and fluoroquinolone class of antibiotics. The core genome MLST was carried out using BacWGSTdb to assess the global phylogenetic relationship of the genome sequence. Supplementary Information: The online version contains supplementary material available at 10.1007/s12088-023-01125-1.
RESUMEN
We report the draft genome sequence of carbapenem-resistant Escherichia coli (CREC-1) of sequence type (ST2083) isolated from a urine sample of a 2-year-old female toddler carrying antimicrobial resistance genes blaNDM-5 and blaCMY-42 which displays resistance against multiple classes of antibiotics notably ß-lactam antibiotics, cephalosporins, and carbapenem and codes for several virulence factors.
RESUMEN
Enterococcus faecalis strain LREF-1 was isolated from a urine sample from India; it belongs to sequence type 28 (ST28), which is associated with nosocomial infections. The strain carries genes for resistance to multiple antibiotics and resistance to various drug classes, including tetracycline, erythromycin, and high-level gentamicin, along with virulence factors.
