RESUMEN
AIM: The aim of this study was to evaluate the efficacy of preoperative prophylactic antibiotics of 2 g of oral amoxicillin on bacteremia following extraction of teeth with periodontal and periapical pathology. MATERIALS AND METHODS: This study was carried out on 160 patients. The patients were divided into four groups of forty patients each: two antibiotic groups, with periodontal and periapical pathology, receiving 2 g of oral amoxicillin preoperatively and two control groups, with periodontal and periapical pathology, receiving no amoxicillin preoperatively. Blood samples were collected before the start of the procedure, intraoperatively, and immediately following extraction of teeth. The collected blood samples were cultured and studied for bacterial growth. RESULTS: In the control group patients with periodontal pathology, 17 out of 40 blood samples showed growth of Streptococcus viridans along with Staphylococcus epidermidis. In the control group patients with periapical pathology, 14 out of 40 blood samples showed growth of S. viridans and Staphylococcus aureus. No growth was observed in both the groups on prophylactic antibiotics with 2 g of oral amoxicillin. CONCLUSION: Bacteremia was found in 40% of the control group patients, while there was no bacteremia present in patients with preoperative administration of 2 g of oral amoxicillin.
RESUMEN
AIMS: The aim of this study was to assess the quality of platelet-rich plasma (PRP) produced by in-house desktop centrifuge method and compare it with that of standardized commercial PRP. MATERIALS AND METHODS: REMI desktop centrifuge was used to prepare PRP and to compare with standardized commercial PRP by calculating the quantity of platelets using Beckman Coulter cell counter in 10 PRP samples and assessing the morphological quality of platelets using JEOL JEM transmission electron microscope (TEM). STATISTICAL ANALYSIS USED: The t test for platelet count in desktop PRP with the test value of therapeutic PRP was 12.618. The P value was <0.001, which was significant statistically. The data followed a normal distribution in normal Q-Q plot for platelet count in desktop centrifuge. So the test samples were not much deviated. RESULTS: The platelet count was lesser than that of standardized commercial PRP. When viewed under JEOL JEM transmission electron microscope, the α granules in platelets were intact and the morphological quality of the PRP was good. CONCLUSIONS: With this study, we have determined that the morphological quality of PRP produced by the in-house desktop centrifuge method is comparable to that of standardized commercial PRP. Though the quantity of platelets was less than 1 million cells/µL, the clinical results were good with desired bone formation, thereby providing good avenue for further research.