Unveiling the Tiny Invaders: A deep dive into microplastics in shrimp - Occurrence, detection and unraveling the ripple effects.

Aquaculture is a rapidly expanding food sector worldwide; it is the farming of fish, shellfish, and other marine organisms. Microplastics (MPs) are small pieces of plastic with a diameter of less than 5 mm that end up in the marine environment. MPs are fragments of large plastics that take years to degrade but can frustrate into small pieces, and some commercially available MPs are used in the production of toothpaste, cosmetics, and aircraft. MPs are emerging contaminants; they are ingested by marine species. These MPs have effects on marine species such as growth retardation and particle translocation to other parts of the body. Recently, MPs accumulation has been observed in shrimps, as well as in a wide range of other scientific reports. So, in this study, we review the presence, accumulation, and causes of MPs in shrimp. These plastics can trophic transfer to other organisms, changes in plastic count, effects on the marine environment, and impacts of MPs on human health were also discussed. It also improves our understanding of the importance of efficient plastic waste management in the ocean, as well as the impact of MPs on marine biota and human health.

Exploring the role and mechanism of potential probiotics in mitigating the shrimp pathogens.

Shrimp aquaculture has rapidly developed into a significant industry worldwide, providing not only financial gain and high-quality food but also tens of thousands of trained and competent workers. Frequent diseases are now regarded as a significant risk factor for shrimp aquaculture, as they have the potential to significantly reduce shrimp production and result in economic losses. Over the years various traditional methods including the use of antibiotics have been followed to control diseases yet unsuccessful. Probiotic is considered potential supplements for shrimps during farming, they may also act beneficially as disease control and increased production. Probiotics are described as a live microbial supplement that benefits the host by modifying the microbial population associated with the host and its ambient. The present state of research about probiotics demonstrates notable impacts on the immune defences of the host's gastrointestinal system, which play a crucial role in safeguarding against diseases and managing inflammation inside the digestive tract. In the past ten years, many studies on probiotics have been published. However, there is a lack of information about the processes by which probiotics exert their effects in aquaculture systems, with only limited elucidations being offered. This study explores the variety of procedures behind the positive effects of probiotics in shrimp culture. These mechanisms include the augmentation of the immune system, control of growth, antagonistic action against pathogens, competitive exclusion, and modification of the gut microbiota. Mechanisms involved in the probiotic mode of action are mostly interlinked. This provides a greater understanding of the importance of probiotics in shrimp culture as an environmentally friendly practice.

Consuming microplastics? Investigation of commercial salts as a source of microplastics (MPs) in diet.

The omnipresence of microplastics (MPs) in marine and terrestrial environments as a pollutant of concern is well established and widely discussed in the literature. However, studies on MP contamination in commercial food sources like salts from the terrestrial environment are scarce. Thus, this is the first study to investigate various varieties of Australian commercial salts (both terrestrial and marine salts) as a source of MPs in the human diet, and the first to detect MPs in black salt. Using Nile red dye, the MPs were detected and counted under light microscopy, further characterised using attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM-EDS). Of all the 90 suspected particles, 78.8% were identified as MPs with a size ranging between 23.2 µm and 3.9 mm. The fibres and fragments constituted 75.78% and 24.22% respectively. Among the tested samples, Himalayan pink salt (coarse) from terrestrial sources was found to have the highest MP load, i.e. 174.04 ± 25.05 (SD) particle/kg, followed by black salt at 157.41 ± 23.13 particle/kg. The average concentration of detected MPs in Australian commercial salts is 85.19 ± 63.04 (SD) per kg. Polyamide (33.8%) and polyurethane (30.98%) were the dominant MP types. Considering the maximum recommended (World Health Organization) salt uptake by adults daily at 5 g, we interpret that an average person living in Australia may be ingesting approximately 155.47 MPs/year from salt uptake. Overall, MP contamination was higher in terrestrial salts (such as black and Himalayan salt) than the marine salt. In conclusion, we highlight those commercial salts used in our daily lives serve as sources of MPs in the diet, with unknown effects on human health.

Experimental infection of Enterocytozoon hepatopanaei parasite (EHP) of penaeid shrimp in Indian marine crabs.

Enterocytozoon hepatopanaei parasite (EHP) is identified as an emerging pathogenic microsporidium parasite in shrimp culture industry. Though the etiology, disease pattern and sustainability of shrimp are well known, significantly less research has been carried out about the disease transmission and symptoms of infected aquatic animals. The present study aims is to determine the disease carrier status of five different species of Indian marine crabs (Scylla olivacea, Scylla serrata, Portunus pelagicus, Ocypode quadrata and Portunus sanquinolentus) using EHP. At the first instance, oral infection and intramuscular injection were performed to determine the susceptibility of the parasite at 50 days post-infection and it was observed that there was no mortality. The experimental infected crabs were confirmed by polymerase chain reaction, bioassay and histopathology. The crabs were EHP-PCR positive at 5th day post-infection (d.p.i) in gills, heart, hepatopancreas, haemolymph and muscle tissue. However, after 5th d.p.i EHP was PCR negative in all the tissue samples. There were no mortalities and histological changes in the negative group and experimental group. Therefore, marine crabs are found to be not suitable hosts for replicating EHP spores but crabs fecal matters are PCR positive till 5th d.p.i. Therefore, marine crabs are having the possibilities of acceptance as a vector for Enterocytozoon hepatopanaei in shrimp. Shrimp farmers need to take necessary action to control this deadly infection in shrimp ponds.

Paeonol Reverses Adriamycin Induced Cardiac Pathological Remodeling through Notch1 Signaling Reactivation in H9c2 Cells and Adult Zebrafish Heart.

Adriamycin is a commonly prescribed chemotherapeutic drug for a wide range of cancers. Adriamycin causes cardiotoxicity as an adverse effect that limits its clinical application in cancer treatment. Several mechanisms have been proposed to explain the toxicity it causes in heart cells. Disruption of inherent cardiac repair mechanism is the least understood mechanism of Adriamycin-induced cardiotoxicity. Adriamycin induces pathological remodeling in cardiac cells by promoting apoptosis, hypertrophy, and fibrosis. We found that Adriamycin inhibited Notch1 in a time- and dose-dependent manner in H9c2 cells. We used Paeonol, a Notch1 activator, and analyzed the markers of apoptosis, hypertrophy, and fibrosis in H9c2 cells in vitro and in adult zebrafish heart in vivo as model systems to study Adriamycin-induced cardiotoxicity. Paeonol activated Notch1 signaling and expression of its downstream target genes effectively in the Adriamycin-treated condition in vitro and in vivo. Also we detected that Notch activation using Paeonol protected the cells from apoptosis, collagen deposition, and hypertrophy response using functional assays. We conclude that Adriamycin induced cardiotoxicity by promoting the pathological cardiac remodeling through inhibition of Notch1 signaling and that the Notch1 reactivation by Paeonol protected the cells and reversed the cardiotoxicity.

Susceptibility of betanodavirus in a newly established vertebra-derived cell line from Mosquitofish (Gambusia affinis).

In the present study, a new cell line from the vertebra of mosquitofish Gambusia affinis was successfully established and characterized. The cell line is named as bone Gambusia affinis (BGA) and subcultured for more than 55 passages in Leibovitz's/L15 medium supplemented with 15% FBS at 28°C. The cell line has a modal chromosome number of 48. Molecular characterization of the partial sequence of the coi gene confirmed the origin of the BGA cell line from mosquitofish. These cells exhibited epithelial morphology confirmed by the cytokeratin marker. The BGA cells showed mineralization of their extracellular matrix when stained with alizarin red and von Kossa stain. BGA cells were found to be susceptible to RGNNV and SJNNV strains of betanodavirus (NNV) showing cytopathic effect with multiple vacuolations in the cells. The RT-PCR confirmed the betanodavirus infections in BGA cells. The SEM micrograph showed the morphological changes observed in the cell during virus infection. The in vivo challenge experiment also showed the viral replicating efficiency in the Gambusia affinis with increasing viral titre. Thus, our present results show that the BGA cell line is a useful tool for isolating betanodavirus and could be used to investigate bone cell differentiation and extracellular matrix mineralization.

Adriamycin inhibits embryonic development in zebrafish through downregulation of Kruppel-like factor4.

Adriamycin is an effective anticancer drug used in a wide range of cancers. Anticancer drugs modulate oncogenes and nodal regulatory molecules that affect cell differentiation and organismal development. In this study, we explore the effect of adriamycin on Kruppel-like factor4 (Klf4), an essential pluripotent factor by choosing zebrafish embryos as a model system. Klf4 is involved in the regulation of cellular growth, proliferation, and differentiation. In zebrafish embryogenesis, Klf4 is a major regulator of differentiation of polster in the anterior mesendoderm region of cells into hatching gland cells. The importance of this study is to check the effect of adriamycin on embryonic development. We found, adriamycin dose dependently altered the gene expression level of Klf4 that occurs in parallel to its detrimental effect on hatching. Supportively, cathepsin L and cyclase-associated protein1 are the other two markers of hatching that are altered along with Klf4.

In Situ Immobilized Sesamol-Quinone/Carbon Nanoblack-Based Electrochemical Redox Platform for Efficient Bioelectrocatalytic and Immunosensor Applications.

Most of the common redox mediators such as organic dyes and cyanide ligand-associated metal complex systems that have been used for various electrochemical applications are hazardous nature. Sesamol, a vital nutrient that exists in natural products like sesame seeds and oil, shows several therapeutic benefits including anticancer, antidiabetic, cardiovascular protective properties, etc. Herein, we introduce a new electrochemical redox platform based on a sesamol derivative, sesamol-quinone (Ses-Qn; oxidized sesamol), prepared by the in situ electrochemical oxidation method on a carbon nanoblack chemically modified glassy carbon electrode surface (GCE/CB@Ses-Qn) in pH 7 phosphate buffer solution, for nontoxic and sustainable electrochemical, electroanalytical, and bioelectroanalytical applications. The new Ses-Qn-modified electrode showed a well-defined redox peak at E o = 0.1 V vs Ag/AgCl without any surface-fouling behavior. Following three representative applications were demonstrated with this new redox system: (i) simple and quick estimation of sesamol content in the natural herbal products by electrochemical oxidation on GCE/CB followed by analyzing the oxidation current signal. (ii) Utilization of the GCE/CB@Ses-Qn as a transducer, bioelectrocatalytic reduction, and sensing of H2O2 after absorbing the horseradish peroxidase (HRP)-based enzymatic system on the underlying surface. The biosensor showed a highly selective H2O2 signal with current sensitivity and detection limit values 0.1303 µA µM-1 and 990 nM, respectively, with tolerable interference from the common biochemicals like dissolved oxygen, cysteine, ascorbic acid, glucose, xanthine, hypoxanthine, uric acid, and hydrazine. (iii) Electrochemical immunosensing of white spot syndrome virus by sequentially modifying primary antibody, antigen, secondary antibody (HRP-linked), and bovine serum albumin on the redox electrode, followed by selective bioelectrochemical detection of H2O2.

An Elegant Analysis of White Spot Syndrome Virus Using a Graphene Oxide/Methylene Blue based Electrochemical Immunosensor Platform.

White spot syndrome virus (WSSV) is a major devastating virus in aquaculture industry. A sensitive and selective diagnostic method for WSSV is a pressing need for the early detection and protection of the aquaculture farms. Herein, we first report, a simple electrochemical immunosensor based on methylene blue dye (MB) immobilized graphene oxide modified glassy carbon electrode (GCE/GO@MB) for selective, quick (35 ± 5 mins) and raw sample analysis of WSSV. The immunosensor was prepared by sequential modification of primary antibody, blocking agent (bovine serum album), antigen (as vp28 protein), secondary antibody coupled with horseradish peroxidase (Ab2-HRP) on the GCE/GO@MB. The modified electrode showed a well-defined redox peak at an equilibrium potential (E1/2), -0.4 V vs Ag/AgCl and mediated H2O2 reduction reaction without any false positive result and dissolved oxygen interferences in pH 7 phosphate buffer solution. Under an optimal condition, constructed calibration plot was linear in a range of 1.36 × 10-3 to 1.36 × 107 copies µL-1 of vp28. It is about four orders higher sensitive than that of the values observed with polymerase chain reaction (PCR) and western blot based WSSV detection techniques. Direct electrochemical immunosensing of WSSV in raw tissue samples were successfully demonstrated as a real sample system.

Multi-Parameter Approach for Evaluation of Genomic Instability in the Polycystic Ovary Syndrome.

BACKGROUND: The polycystic ovary syndrome (PCOS), characterized by hyperandrogenism and chronic anovulation, is a common endocrine disorder in women. PCOS, which is associated with polycystic ovaries, hirsutism, obesity and insulin resistance, is a leading cause of female infertility. In this condition there is an imbalance in female sex hormones. All the sequelae symptoms of PCOS gradually lead to cancer in the course of time. It is heterogeneous disorder of unknown etiology so it is essential to find the exact cause. MATERIALS AND METHODS: In this study both invasive and non-invasive techniques were employed to establish the etiology. Diagnosis was based on Rotterdam criteria (hyperandrogenism, ovulatory dysfunction, PCOM) and multiparameters using buccal samples and dermatoglypic analysis and cytogenetic study for 10 cases and four age and sex matched controls. RESULTS: In clinical analysis we have observed the mean value of total testosterone level was 23.6nmol/L, total hirsutism score was from 12-24, facial acne was found in in 70% patients with 7-12 subcapsular follicular cysts, each measuring 2-8 mm in diameter. In dermatoglypic analysis we observed increases in mean value (45.9°) of ATD angle when compared with control group and also found increased frequency (38%) of Ulnar loops on both fingers (UU), (18%) whorls on the right finger and Ulnar loop on left finger (WU) and (16%) arches on right and left fingers (AA) were observed in PCOS patients when compared with control subjects. Features which could be applied as markers for PCOS patients are the presence of Ulnar loops in middle and little fingers of right and left hand. The buccal micronucleus cytome assay in exfoliated buccal cells, we found decrease in frequency of micronuclei and significant increases in frequency of karyolysed nuclei in polycystic ovarian syndrome patients. Chromosome aberration analysis revealed a significant increase in frequency of chromosome aberrations (CAs) in PCOS patients when compared with controls. CONCLUSIONS: From this present work it can be concluded that non-invasive technique like dermatoglypics analysis and buccal micronucleus cytome assays with exfoliated buccal cell can also be effective biomarkers for PCOS, along with increased CAs in lymphocytes as a sign of genetic instability. There is a hypothesis that micronuclei and chromosomal aberrations could have a predictive value for cancer. From this present work it can be concluded to some extent that non-invasive technique like dermatoglypics and buccal cell analysis can also be effective for diagnosis.