Prussian blue analogues of Ni-Co-MoS2 nanozymes with high peroxidase like activity for sensitive detection of glyphosate and copper.

The rational development of efficient nanozymes for the colorimetric detection of targets is still challenging. Herein, Prussian blue analogues of Ni-Co-MoS2 nano boxes were fabricated for colorimetric detection of glyphosate and copper ions owing to its peroxidase like activity. At the sensing system, the Ni-Co-MoS2 nano boxes display high peroxidase activity, which could catalytically oxidize the colourless TMB to blue colour oxTMB. In presence of glyphosate in this sensing system the blue colour is diminished, ascribed to the inhibit the catalytic activity of Ni-Co-MoS2 nano boxes. Concurrently, the addition of copper ion, which result in blue colour was reappear due to the generation of glyphosate-copper complex formation. The Ni-Co-MoS2 nano boxes based colorimetric sensing platform was developed to sensitive detection of glyphosate and copper ions with low detection limit of 3 nM for glyphosate and 3.8 nM for copper. This method also displays satisfactory outcomes from real samples analysis and its good accuracy. Therefore, this work provides a great potential for rapid detection of the targets from the environments.

A Turn-ON fluorometric biosensor based on ssDNA immobilized with a metal phenolic nanomaterial for the sequential detection of Pb(ii) and epirubicin cancer drug.

In this paper, we propose a fluorescent biosensor for the sequential detection of Pb2+ ions and the cancer drug epirubicin (Epn) using the interactions between label-free guanine-rich ssDNA (LFGr-ssDNA), acridine orange (AO), and a metal-phenolic nanomaterial (i.e., nano-monoclinic copper-tannic acid (NMc-CuTA)). An exploration of the sensing mechanism shows that LFGr-ssDNA and AO strongly adsorb on NMc-CuTA through π-π stacking and electrostatic interactions, and this results in the fluorescence quenching of AO. In order to sense the target Pb2+, initially, LFGr-ssDNA specifically binds with Pb2+ ions to form a G4 complex (G-Pb2+-G base pair), which was released from the surface of NMc-CuTA with strong AO fluorescence enhancement (Turn-ON). The subsequent addition of a biothiol, like cysteine (Cys), to the G4 complex decreases the fluorescence, as the Pb2+ ions released from the G4 complex have a higher interaction affinity with the sulfur atoms of Cys; this further induces the unwinding of the G4 complex to form LFGr-ssDNA. Finally, Epn was added to this, which intercalates with LFGr-ssDNA to form a G4 complex via G-Epn-G, resulting in fluorescence recovery (Turn-ON). Accordingly, the Turn-ON fluorescent probe had subsequent limits of detection of 1.5 and 5.6 nM for Pb2+ and Epn, respectively. Hence, the reported NMc-CuTA-based sensing platform has potential applications for the detection of Pb2+ and Epn in real samples with good sensitivity and selectivity.