Oral Administration of Ethanolic Extract of Shrimp Shells-Loaded Liposome Protects against Aß-Induced Memory Impairment in Rats.

Alzheimer's disease is characterized by a progressive loss of memory and cognition. Accumulation of amyloid-beta (Aß) in the brain is a well-known pathological hallmark of the disease. In this study, the ethanolic extract of white shrimp (Litopenaous vannamei) shells and the ethanolic extract-loaded liposome were tested for the neuroprotective effects on Aß1-42-induced memory impairment in rats. The commercial astaxanthin was used as a control. Treatment with the ethanolic extract of shrimp shells (EESS) at the dose of 100 mg/kg BW showed no protective effect in Aß-treated rats. However, treatment with an EESS-loaded liposome at the dose of 100 mg/kg BW significantly improved memory ability in Morris water maze and object recognition tests. The beneficial effect of the EESS-loaded liposome was ensured by the increase of the memory-related proteins including BDNF/TrkB and pre- and post-synaptic protein markers GAP-43 and PSD-95 as well as pErk1/2/Erk1/2 in the cortex and hippocampus. These findings indicated the neuroprotective effects of the EESS-loaded liposome on Aß-induced memory impairment in rats. It produced beneficial effects on learning behavior probably through the function of BDNF/TrkB/pErk1/2/Erk1/2 signaling pathway and subsequently the upregulation of synaptic proteins. The present study provided evidence that the neuroprotective property of the ESSE-loaded liposome could be a promising strategy for AD protection.

Improved cholesterol depletion with enhanced astaxanthin and polyunsaturated fatty acids of lipid from Pacific white shrimp cephalothorax using prior ethanolic separation of polar lipid and ß-Cyclodextrin.

Shrimp lipid (SL) from Pacific white shrimp (Litopenaeus vannamei) cephalothorax was subjected to ethanol separation with subsequent cholesterol removal. Around 98.4% of cholesterol was removed from cholesterol rich polar lipid fraction (PLF), in which PLF/ß cyclodextrin (ß-CD)/mixed solvents (ethyl acetate/water,1:1) at the ratio of 1:10:20 (w/w/v) were used. Thereafter, PLF with lowered cholesterol was combined with non-polar fraction rich in triglycerides to obtain lowered cholesterol shrimp lipid (LC-SL). Astaxanthin content in LC-SL was augmented by three-fold, compared to that found in SL. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents of LC-SL were also significantly increased, contrasted with SL. Peroxide value and phospholipids were decreased in LC-SL (4.56 ± 0.15 meq/kg and 9.94 ± 1.9%) compared to those of SL (4.80 ± 0.25 meq/kg and 49.11 ± 2.1%), while TBARS and p-Anisidine values remained unchanged. However, conjugated dienes and free fatty acids were augmented, plausibly due to hydrolysis. FTIR spectra confirmed the increased degree of unsaturation of lipids. Thus, the lowered cholesterol shrimp lipid could be used as functional foods or nutraceutical for health promotion.

Whole Wheat Crackers Fortified with Mixed Shrimp Oil and Tea Seed Oil Microcapsules Prepared from Mung Bean Protein Isolate and Sodium Alginate.

Shrimp oil (SO) rich in n-3 fatty acids and astaxanthin, mixed with antioxidant-rich tea seed oil (TSO), was microencapsulated using mung bean protein isolate and sodium alginate and fortified into whole wheat crackers. SO and TSO mixed in equal proportions were emulsified in a solution containing mung bean protein isolate (MBPI) and sodium alginate (SA) at varied ratios. The emulsions were spray-dried to entrap SO-TSO in MBPI-SA microcapsules. MBPI-SA microcapsules loaded with SO-TSO showed low to moderately high encapsulation efficiencies (EE) of 32.26-72.09% and had a fair flowability index. Two selected microcapsules with high EE possessed the particle sizes of 1.592 and 1.796 µm with moderate PDI of 0.372 and 0.403, respectively. Zeta potential values were -54.81 mV and -53.41 mV. Scanning electron microscopic (SEM) images indicated that microcapsules were spherical in shape with some shrinkage on the surface and aggregation took place to some extent. Fourier transform infrared (FTIR) and differential scanning calorimetry (DSC) analyses of samples empirically validated the presence of SO-TSO in the microcapsules. Encapsulated SO-TSO showed superior oxidative stability and retention of polyunsaturated fatty acids (PUFAs) to unencapsulated counterparts during storage of 6 weeks. When SO-TSO microcapsules were fortified in whole wheat crackers at varying levels (0-10%), the crackers showed sensorial acceptability with no perceivable fishy odor. Thus, microencapsulation of SO-TSO using MBPI-SA as wall materials could be used as an alternative carrier system, in which microcapsules loaded with PUFAs could be fortified in a wide range of foods.

Advancements in liposome technology: Preparation techniques and applications in food, functional foods, and bioactive delivery: A review.

Liposomes play a significant role in encapsulation of various bioactive compounds (BACs), including functional food ingredients to improve the stability of core. This technology can be used for promoting an effective application in functional food and nutraceuticals. Incorporation of traditional and emerging methods for the developments of liposome for loading BACs resulted in viable and stable liposome formulations for industrial applications. Thus, the advance technologies such as supercritical fluidic methods, microfluidization, ultrasonication with traditional methods are revisited. Liposomes loaded with plant and animal BACs have been introduced for functional food and nutraceutical applications. In general, application of liposome systems improves stability, delivery, and bioavailability of BACs in functional food systems and nutraceuticals. This review covers the current techniques and methodologies developed and practiced in liposomal preparation and application in functional foods.

Recovery, reusability and stability studies of beta cyclodextrin used for cholesterol removal from shrimp lipid.

Beta cyclodextrin (ß-CD) was used for cholesterol removal from shrimp lipid using ethyl acetate and water as solvents. The cholesterol incorporating ß-CD complex (ß-CD-CL) was collected and ß-CD recovery was performed using a ß-CD-CL : ethanol mixture (1 : 15 ratio) with the aid of ultrasonication and a water bath at 55 °C for 40 min. Recycled ß-CD (R-ß-CD) was compared with pure ß-CD (P-ß-CD) for the reusability of cholesterol removal from shrimp lipid. R-ß-CD showed 94% cholesterol removal, while 95% was achieved for P-ß-CD. Differential Scanning Calorimetry (DSC) showed a slight decrease in the melting point of R-ß-CD. Nevertheless, FTIR and NMR results revealed that functional groups and the proton spectrum of R-ß-CD was negligibly altered. Fatty acid contents of treated oil were slightly higher when treated with R-ß-CD than those of the lipid subjected to P-ß-CD treatment. Reusability of ß-CD could be achieved as confirmed by the maintained capacity in cholesterol removal and unaltered structure.

Comparative Study of Astaxanthin, Cholesterol, Fatty Acid Profiles, and Quality Indices Between Shrimp Oil Extracted From Hepatopancreas and Cephalothorax.

Shrimp oil from two different portions of Pacific white shrimp including cephalothorax and hepatopancreas was extracted using the mixture of hexane/isopropanol (1:1). The extracted oils from the cephalothorax (CPO) and hepatopancreas (HPO) were characterized for astaxanthin content, cholesterol levels, and fatty acid profiles. Nutrition indices of CPO and HPO were also compared. CPO had lower extraction yield (3.2 ± 0.1%, wet weight basis) than HPO (11.1 ± 0.5%, wet weight basis). High-performance liquid chromatography results indicated that the astaxanthin content in HPO was higher, compared to that of CPO. Nevertheless, the cholesterol level in HPO was 70% lower than that of CPO. Fatty acid profiles of HPO and CPO demonstrated that the polyunsaturated fatty acid (PUFA) content in HPO was higher than that of CPO. The amount of docosahexaenoic acid in the former was ~2 times higher than that of the latter. HPO contained 42.76 ± 0.36% PUFA, whereas PUFA content of CPO was 35.27 ± 0.19%. On the other hand, saturated fatty acids (SFA) were more pronounced in CPO (38.44 ± 0.26%) than HPO (30.82 ± 0.55%). Based on nutrition indices, namely, atherogenicity index, thrombogenicity index, hypocholesterolemic/hypercholesterolemic (h/H) ratio, and PUFA/SFA ratio, HPO possessed higher health benefit than CPO. The oxidation status of CPO and HPO measured in terms of peroxide value, thiobarbituric acid reactive substances, anisidine value, and conjugated dienes indicated that higher primary oxidation products were present in CPO, whereas HPO exhibited more secondary oxidation compounds. Fourier transform infrared spectra further substantiated the presence of oxidation products in CPO and HPO. Liquid chromatography-mass spectrometry identification showed the enhanced levels of phospholipids and glycolipids in the ethanolic fraction of CPO. Overall, HPO with a higher yield was more beneficial in terms of health benefits than CPO.