An immobilization of 2-(Aminomethyl) thiazole on multi-walled carbon nanotubes used for rapid extraction of manganese ions in hepatic patients.

A new method based on the immobilization of 2-(Aminomethyl) thiazole on the multi-walled carbon nanotubes (AMTZ@MWCNTs) was used to extract manganese (Mn) in the human blood, serum, and urine samples. First, 20 mg of AMTZ@MWCNTs, 0.2 mL of acetone, and 0.1 g of ionic liquid (IL) were completely mixed and injected into 2.0 mL human samples by a microscale syringe at pH 5.5. After shaking and centrifuging, the Mn ions were extracted and separated through the ultrasound-assisted- ionic liquid-dispersive micro solid-phase extraction (UAS-IL-D-µ-SPE) before being determined by the graphite furnace atomic absorption spectrometry (GF-AAS). According to the results, manganese in the blood of hepatic patients had higher concentrations than healthy people (Aged 25-60, 50 N). The Mn adsorption capacities for the AMTZ@MWCNTs and MWCNTs adsorbents were achieved at 192.5 mg/g and 26.3 mg/g, respectively. In the high enrichment factor (HEF), the limit of detection (LOD), linear range (LR), and mean relative standard division (RSD%) were calculated at 15 ng/L, 0.05-3.8 µg/L, and 2.34, respectively (n = 10). The methodology was validated using certified reference material (CRM) and spiking standard solutions to human samples.

Simultaneously speciation and determination of manganese (II) and (VII) ions in water, food, and vegetable samples based on immobilization of N-acetylcysteine on multi-walled carbon nanotubes.

A novel method based on the immobilization of N-acetylcysteine on chloro-functionalized multi-walled carbon nanotubes (MWCNTs@NAC) was used for the speciation of manganese ions [Mn (II) and Mn(VII)] in water samples. Also, the total manganese (TMn) in vegetables and food samples was determined by the AT-FAAS. By ultrasound-assisted-dispersive ionic liquid trap micro solid-phase extraction (UA-DILT-µ-SPE), the Mn (II)/Mn(VII) ions were extracted in the presence of MWCNTs@NAC for 50 mL of water samples at a pH of 6.5 and 3.0, respectively. The adsorption capacity of MWCNTs@NAC for Mn(II) and Mn(VII) ions was obtained at 146.7 mg g-1 and 138.8 mg g-1, respectively. Under the optimized conditions, the detection limits (LOD), linear range (LR), and enrichment factor (EF) for Mn(II) and Mn(VII) ions were obtained (0.12 µg L-1; 0.14 µg L-1), (0.48-36 µg L-1; 0.55-38.1 µg L-1) and (100.2; 94.5), respectively. The proposed methodology was successfully validated by the CRM samples.

A review of hydrogel systems based on poly(N-isopropyl acrylamide) for use in the engineering of bone tissues.

Bone fracture is usually a medical condition where occurred by high force impact or stress. Recent advances to repair damaged or diseased bone tissues employs three-dimensional (3D) polymer matrices. This review aims to investigate the potential of injectable, dual thermally, and chemically gelable N-isopropyl acrylamide-based hydrogels to deliver scaffold, cells, and growth factors in vitro and in vivo.

Rapid Speciation of Lead in Human Blood and Urine Samples Based on MWCNTs@DMP by Dispersive Ionic Liquid-Suspension-Micro-Solid Phase Extraction.

An efficient sorbent based on 2,3-dimercapto-1-propanol immobilized on multi-wall carbon nanotubes (MWCNTs@DMP) was developed for separation/speciation of organic and inorganic lead (alkyl-Pb, Pb2+) in human blood, urine, and water samples by dispersive ionic liquid-suspension-micro-solid phase extraction (DIL-S-µ-SPE). By procedure, the MWCNTs@DMP as solid phase, acetone, and ionic liquid (IL, [HMIM][PF6]) were mixed and injected to 10 mL of the liquid phase at pH = 6.5. After shaking, the Pb(II) was extracted in MWCNTs@DMP and settled down in a conical tube with IL by centrifuging (Pb2+→: SH-SiO2@CNTs). The lead (Pb2+) was back-extracted from sorbent/IL in acidic pH and measured by atom trap atomic absorption spectrometry (AT-AAS). In addition, the organic lead (R-Pb, alkyl lead) converted to Pb(II) and total lead (T-Pb) was determined in the same conditions by UV radiation in 95 °C. Under the optimal conditions, the linear range (9.5-480 µg L-1), LOD (3.2 µg L-1), and enrichment factor (10.4) were obtained (RSD < 5%). The adsorption capacity of the MWCNTs@DMP and MWCNTs was achieved as 191.6 mg g-1 and 25.8 mg g-1, respectively. The method was validated by standard reference materials (SRM 1643d, SRM 955, and SRM 2668), ET-AAS, and ICP-MS analysis in real samples. Graphical abstract.

Experimental and theoretical insights into the synthesis of α-aminoalkyl naphthol derivatives catalyzed by a manganese complex immobilized on multi-walled carbon nanotubes.

The full details of the synthesis of α-aminoalkyl naphthol derivatives promoted by a manganese complex immobilized on multi-walled carbon nanotubes (CNTs@Mn-bpy) are described, with a particular focus on theoretical mechanistic aspects. After characterization studies on the nanocatalyst prepared from a homogeneous manganese complex, we optimized the catalytic reaction conditions, and the heterogeneous nanocatalyst showed important features such as higher efficiency and reusability compared to other reported catalysts. α-Aminoalkyl naphthol derivatives were obtained in excellent chemical yields and short reaction times and with high turnover numbers. In order to better understand the effect of CNT@Mn-bpy as a nanocatalyst on the reaction mechanism, the final step of the described synthesis has been investigated using density functional theory (DFT). Compared to the uncatalyzed reaction, the activation energy of the catalyzed reaction is lowered by 34.07 kcal mol-1. Also, our theoretical calculation shows that if the Mn metal of CNT@Mn-bpy is replaced with the Cu metal, the catalytic effect of this nanocatalyst on the activation energy of the studied reaction was significantly decreased.

Catalytic application of new manganese Schiff-base complex immobilized on chitosan-coated magnetic nanoparticles for one-pot synthesis of 3-iminoaryl-imidazo[1,2-a]pyridines.

In this paper, new chitosan (CS) coated magnetic iron oxide nanoparticles (MNPs) supported manganese Schiff-base complex was designed, synthesized and screened as an efficient, reusable nanocatalyst by the three-component reaction of various aldehyde derivatives with trimethylsilyl cyanide and 2-aminopyridine for the synthesis of 3-iminoaryl-imidazo[1,2-a]pyridine (IAIP) derivatives. Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), vibrating sample magnetometry (VSM) and inductively coupled plasma atomic emission spectroscopy (ICP-AES) have been used to characterize the nanocatalyst before reaction. For the first time, earlier than immobilizing of manganese complex, the chitosan-coated Fe3O4 nanoparticles prepared through covalent bond formation between CS and chlorosilane-modified MNPs which were treated with triethylamine. It is a promising nanocatalyst providing high yield, short reaction times, and excellent turnover number. The recycle studies show a significant loss of activity after recycling six times.

A novel biostructure sorbent based on CysSB/MetSB@MWCNTs for separation of nickel and cobalt in biological samples by ultrasound assisted-dispersive ionic liquid-suspension solid phase micro extraction.

An efficient method based on CysSB/MetSB@MWCNTs as a novel bio structure material was used for determination/separation of nickel and cobalt (Ni and Co) in human samples by ultrasound assisted-dispersive ionic liquid-suspension solid phase micro extraction (USA-DIL-SSPME). In this procedure, CysSB/MetSB@MWCNTs suspended in 1-butyl-2,3-dimethylimidazolium hexafluorophosphate ([BDMIM][PF6]) and mixture dispersed to 10 mL of blood samples at optimized pH by injecting. Then, the Co/Ni (II) was extracted with CysSB/MetSB@MWCNTs without any ligands and settled down in conical tube by IL [Ni/Co→:SMWCNTs]. After back extraction of ions from remaining solution, the concentration of Co/Ni was determined by electro thermal atomic absorption spectrometry (ET-AAS). By optimizing, the linear range, detection limit and enrichment factor of CysSB @MWCNTs were obtained (0.1-3.4 µg L-1; 0.08-3.2 µg L-1), (0.028 µg L-1; 0.022 µg L-1) and (50.2; 48.7) for Ni and Co ions in human biological samples, respectively (RSD<5%). The adsorption capacity of CysSB@MWCNTs for Ni and Co ions was 226.7 mg g-1 and 193.3 mg g-1, respectively which was higher than MetSB@MWCNTs. The standard reference materials (NIST, SRM) and ICP-MS were used for validation of methodology.

Synthesis of 1-(α-aminoalkyl)-2-naphthol and α-aminonitrile derivatives with molybdenum Schiff base complex covalently bonded on silica-coated magnetic nanoparticles and DNA interaction study of one type of derivatives using computational and spectroscopic methods.

An air- and moisture-stable molybdenum Schiff base complexes immobilized on magnetic iron oxide nanoparticles with a core-shell structure was developed for utilization as a new heterogeneous catalyst. The surface, structural and magnetic characteristics of the nanomaterials were characterized by using Fourier transform infrared (FT-IR) spectroscopy, X-ray powder diffraction (XRD), vibrating sample magnetometry (VSM), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and inductively coupled plasma atomic emission spectroscopy (ICP-AES). In application point of view, an ultrasonic assisted one-pot multicomponent protocol for the synthesis of 1-(α-aminoalkyl)-2-naphthol derivatives have been demonstrated under mild condition with short reaction times, high yields and TON values up to 570. To survey the generality of the procedure, we studied the synthesis of α-aminonitrile derivatives with different aldehydes, trimethylsilyl cyanide (TMSCN) and aniline under the same conditions. Additionally, binding interaction of 1-(phenyl(pyridin-2-ylamino)methyl)naphthalen-2-ol (AMAN-1) with various types of rigid DNA and HSA has been investigated by molecular modeling study. In vitro studies under physiological conditions showed that the desired derivative interacts with calf-thymus DNA (ct-DNA) via an intercalative binding mode.

Preparation of a highly stable drug carrier by efficient immobilization of human serum albumin (HSA) on drug-loaded magnetic iron oxide nanoparticles.

Albumin immobilized nanoparticles are known to be biodegradable, easy to prepare and reproducible for drug delivery systems. In summary, we have synthesized a new drug carrier using modified iron oxide nanoparticles. The synthesized drug carrier was characterized by X-ray powder diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FT-IR), vibrating sample magnetometry (VSM) and energy-dispersive X-ray spectroscopy (EDX). Three different drugs were loaded on the modified iron oxide nanoparticles and then human serum albumin (HSA) immobilized on the iron oxide nanoparticles. In addition, the in-vitro antiproliferative activity of Fe3O4@SiO2@Nev@HSA nanoparticles against Hela cancer cell line using MTT colourimetric assay was compared with nevirapine. The results show that Fe3O4@SiO2@Nev@HSA nanoparticles in comparison to nevirapine itself have more effective antiproliferative activity on Hela cancer cell lines. The IC50 value for Fe3O4@SiO2@Nev@HSA nanoparticles was 59.20 µg/ml, which is close to the antiproliferative activity of anti-cancer gefitinib with IC50 value of 76.24 µg/ml. Moreover, in vitro calf thymus DNA (ct-DNA) binding studies were investigated by various spectroscopy techniques.

Improving antiproliferative effect of the nevirapine on Hela cells by loading onto chitosan coated magnetic nanoparticles as a fully biocompatible nano drug carrier.

The freshly prepared magnetic iron oxide nanoparticles (MIONPs) were coated with SiO2 and then modified with a Si-based linker (SiL) having chlorine atom at the end of its chain. The resulting chlorine functionalized MIONPs were bonded to chitosan (CT) in trimethylamine solution. Then nevirapine (Nev) drug was loaded into above CT-SiL-MIONPs system and resulting Nev-loaded magnetic nanoparticles, Nev@CT-SiL-MIONPs, studied using different techniques. Furthermore, the value of Nev loading efficiency and also controlled delivery effect of Nev@CT-SiL-MIONP particles was determined by UV-vis spectrometer. Interestingly, the above nanomaterial showed a superparamagnetic property with a saturation magnetization value of 35.66 emu/g, indicating that it has an excellent potential application in the treatment of cancer using magnetic targeting drug delivery technology. Furthermore, the in-vitro antiproliferative activity of Nev@CT-SiL-MIONPs against cancer cell line (Hela) was compared with nevirapine using MTT colourimetric assay. The Nev-loaded magnetic nanoparticles were shown to be more effective antiproliferative on Hela cancer cell lines than nevirapine itself. Moreover, in vitro ct-DNA binding studies were investigated by UV-Vis and competitive fluorescence spectroscopies. The results showed that DNA aggregated on Nev-loaded nanoparticles via groove binding mode.