Electrospun EU/HPMC nanofibers decorated by ZIF-8 nanoparticle as the advanced electrochemical biosensor modifier for sensitive and selective detection of c-MET cancer biomarker in human plasma sample.

This research presents a selective and sensitive electrochemical biosensor for the detection of the mesenchymal-epithelial transition factor (c-MET). The biosensing is based on a modification of the SPCE (screen-printed carbon electrode) with the electrospun nanofiber containing eudragit (EU), hydroxypropyl methylcellulose (HPMC), and Zeolite imidazolate frameworks (ZIF-8) nanoparticles. EU/HPMC/ZIF-8 nanofibers have presented a high capability of electron transfer, and more active surface area than bare SPCE due to synergistic effects between EU, HPMC, and ZIF-8. On the other hand, EU/HPMC nanofibers provided high porosity, flexible structures, high specific surface area, and good mechanical strength. The presence of ZIF-8 nanoparticles improved the immobilization of anti-c-MET on the modified SPCE and also resulted in increasing the conductivity. By c-MET incubation on the modified SPCE, c-MET was connected to anti-c-MET, and consequently the electrochemical signal of [Fe(CN)6]3-/4- as the anion redox probe was reduced. In order to investigate the structural and morphological characteristics and elemental composition of electrospun nanofibers, various characterization methods including FE-SEM, XRD, FTIR, and EDS were used. Under optimum conditions with a working potential range -0.3-0.6 V (vs. Ag/AgCl), linear range (LR), correlation coefficient (R2), sensitivity, and limit of detection (LOD) were acquired at 100 fg/mL-100 ng/mL, 0.9985, 53.28 µA/cm2.dec, and 1.28 fg/mL, respectively. Moreover, the mentioned biosensor was investigated in a human plasma sample to determine c-MET and showed ideal results including reproducibility, stability, and good selectivity against other proteins.

Polycaprolactone/polyacrylic acid/graphene oxide composite nanofibers as a highly efficient sorbent to remove lead toxic metal from drinking water and apple juice.

Due to the characteristics of electrospun nanofibers (NFs), they are considered a suitable substrate for the adsorption and removal of heavy metals. Electrospun nanofibers are prepared based on optimized polycaprolactone (PCL, 12 wt%) and polyacrylic acid (PAA, 1 wt%) polymers loaded with graphene oxide nanoparticles (GO NPs, 1 wt%). The morphological, molecular interactions, crystallinity, thermal, hydrophobicity, and biocompatibility properties of NFs are characterized by spectroscopy (scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Thermogravimetric analysis), contact angle, and MTT tests. Finally, the adsorption efficacy of NFs to remove lead (Pb2+) from water and apple juice samples was determined using inductively coupled plasma optical emission spectroscopy (ICP-OES). The average diameter for PCL, PCL/PAA, and PCL/PAA/GO NFs was 137, 500, and 216 nm, respectively. Additionally, the contact angle for PCL, PCL/PAA, and PCL/PAA/GO NFs was obtained at 74.32º, 91.98º, and 94.59º, respectively. The cytotoxicity test has shown non-toxicity for fabricated NFs against the HUVEC endothelial cell line by more than 80% survival during 72 h. Under optimum conditions including pH (= 6), temperature (25 °C), Pb concentration (25 to 50 mg/L), and time (15 to 30 min), the adsorption efficiency was generally between 80 and 97%. The adsorption isotherm model of PCL/PAA/GO NFs in the adsorption of lead metal follows the Langmuir model, and the reaction kinetics follow the pseudo-second-order. PCL/PA/GO NFs have shown adsorption of over 80% in four consecutive cycles. The adsorption efficacy of NFs to remove Pb in apple juice has reached 76%. It is appropriate and useful to use these nanofibers as a high-efficiency adsorbent in water and food systems based on an analysis of their adsorption properties and how well they work.

Novel electrospun nanofibers based on gelatin/oxidized xanthan gum containing propolis reinforced by Schiff base cross-linking for food packaging.

Gelatin-based electrospun fibers are promising materials for food packaging but suffer from high hydrophilicity and weak mechanical properties. To overcome these limitations, in the current study, gelatin-based nanofibers were reinforced by using oxidized xanthan gum (OXG) as a crosslinking agent. The nanofibers' morphology was investigated through SEM, and the observations showed that the fibers' diameter was decreased by enhancing OXG content. The resultant fibers with more OXG content exhibited high tensile stress so the optimal sample obtained showed a tensile stress of 13.24 ± 0.76 MPa, which is up to 10 times more than neat gelatin fiber. Adding OXG to gelatin fibers reduced water vapor permeability, water solubility, and moisture content properties while increasing thermal stability and porosity. Additionally, the nanofibers containing propolis displayed a homogenous morphology with high antioxidant and antibacterial activities. In general, the findings suggested that the designed fibers could be used as a matrix for active food packaging.

Investigation of wound healing efficiency of multifunctional eudragit/soy protein isolate electrospun nanofiber incorporated with ZnO loaded halloysite nanotubes and allantoin.

One significant aspect of the current therapeutic agents employed in wound healing involves the engineering of nano polymeric scaffolds to mimic the properties of extracellular matrix (ECM). The present work aimed to prepare and evaluate Eudragit® L100 (EU) nanofibers in combination with soy protein isolate (SPI). Allantoin (Ala) with a 2 wt% was encapsulated as a model drug renowned for its anti-inflammatory and antioxidant agents. Moreover, the synthesized ZnO-halloysite nanotubes (ZHNTs) with different concentrations of 1, 3, and 5 wt% were incorporated into the EU/SPI/Ala nanofiber as a reinforcing filler and a remarkable antibacterial agent. The scanning electron microscope (SEM) analysis showed that by increasing the weight percentage of SPI from 1 % to 2.5 %, the average diameter of nanofibers decreased from 132.3 ± 51.3 nm to 126.7 ± 47.2 nm. It was 223.5 ± 95.6 nm for nanofibers containing 5 wt% ZHNTs (the optimal sample). The evaluation of in vitro release kinetics of Ala for 24 h, showed a burst release during the first 2 h and a sustained release during the subsequent times. Moreover, the structure, crystallinity, and thermal stability of synthesized nanofibers were characterized by Fourier Transform Infrared Spectrometry (FTIR), X-ray diffraction (XRD), and Thermo gravimetric analysis (TGA), respectively. In vitro degradation and mechanical characteristics of these nanofibers were studied. Furthermore, the capability of the nanofibers for cell proliferation was revealed through the MTT test and field emission scanning electron microscopy (FESEM) images of cell attachment. The antimicrobial activity of EU/SPI/Ala/ZHNTs showed that this sample with high ZHNTs content (5 w%t) had the most remarkable antibacterial activity against S. aureus. The results revealed that EU/SPI/Ala/ZHNTs mats could be promising potential wound dressings.

Evaluation of therapeutic effects of nanofibrous mat containing mycophenolate mofetil on oral lichen planus: In vitro and clinical trial study.

Objectives: Recently, topical drug delivery system has gained increasing interest in the treatment of oral lesions. Lichen planus is a chronic inflammatory disease affecting mucous membranes and skin. The current study aimed to fabricate a drug delivery system containing mycophenolate mofetil for the treatment of oral lichen planus lesions. Methods: Firstly, a nanofibrous mat containing mycophenolate mofetil, zinc oxide nanoparticles, and aloe vera was designed and fabricated. The antimicrobial, cytocompatibility, anti-inflammatory, and antioxidative characteristics of fabricated scaffolds were evaluated. Then, this nanofibrous mat was applied to 12 patients suffering from bilateral erythematous/erosive Oral Lichen planus (OLP) lesions for 2 weeks. The treatment outcomes, including oral symptoms and lesion size, were compared with the routine topical treatment of these lesions; Triamcinolone ointment. Results: The characterization of nanofibrous mat approved the successful fabrication of scaffolds. The fabricated nanofibers showed notable antimicrobial activity. The amounts of TNF 𝛼, IL6, and reactive oxygen species (ROS) of stimulated human gingival fibroblasts were decreased after exposure to NFs/Myco/Alv/ZnO scaffolds. The clinical trial results demonstrated the same therapeutic effects compared to the commercial ointment, while the symptoms of patients were significantly improved in the mats group.Significance. Considering the successful results of this study, the application of nanofibrous mat can be a promising product for improving treatment outcomes of OLP.

Optimization and characterization of a novel tea tree oil-integrated poly (ε-caprolactone)/soy protein isolate electrospun mat as a wound care system.

A set of poly (ε-caprolactone)/soy protein isolate (PCL/SPI) mats with different ratios of PCL to SPI was fabricated using the electrospinning method. The mat with PCL to SPI ratio of 95:5 (PS 95:5) had the narrowest nanofibers, the highest percentage of porosity, the lowest swelling ratio, the least vapor transmission, and the slowest degradation rate among the prepared mats. The hemolysis assay indicated that all mats can be considered biocompatible biomaterials. In continue, three different weight ratios of tea tree oil (TTO) were loaded into the PS 95:5 mat. The release profiles illustrated that higher amounts of TTO could be released in an acidic environment. The antioxidant activity of the mats increased by the increase in their TTO content. The cell viability test, cell adhesion images, and live/dead assay of TTO-loaded mats affirmed that all fabricated mats were biocompatible. The scratch wound assay expressed that TTO accelerates the rate of wound closure. The TTO-loaded mats illustrated antibacterial activity against both Escherichia coli and Staphylococcus aureus bacteria. The obtained outcomes revealed that TTO-loaded PCL/SPI mats can be considered promising potential wound dressings.

Immobilization of α-amylase in ethylcellulose electrospun fibers using emulsion-electrospinning method.

α-Amylase encapsulated in water in oil (W/O) emulsion was prepared using poly ethylene glycol (PEG 10000) (2%w/v) as water phase and ethylcellulose (EC) in ethyl acetate as oil phase at the ratio of 10:90 v/v. Next, the electrospun fibers were prepared by mixing enzyme loaded emulsion with EC solution (20%w/v) in acetic acid/ethyl acetate (20:80 v/v) at the 2:1 ratio. The emulsion showed good physical stability. The immobilized enzyme showed high activity across a board range of pHs and temperatures. The storage stability of the immobilized enzyme was 2 fold of free enzyme activity after 45 days. The residual activity of immobilized α-amylase onto of fibers after 10 and 15 repeated cycles, was approximately 100% and 50%, respectively. The results of this study indicated that the α-amylase loaded EC fibers have acceptable activity against harsh conditions and excellent reusability.

Electrospun nanofiber based on Ethyl cellulose/Soy protein isolated integrated with bitter orange peel extract for antimicrobial and antioxidant active food packaging.

The present work was aimed to produce a novel bioactive nanofiber (NFs) based on Ethyl cellulose (EC), Soy protein isolated (SPI), and containing Bitter orange peel extract (BOPE) by electrospinning technology. The EC/SPI NFs were formulated with different weight ratios of 1:1, 2:1, and 1:2 denoted as ES11, ES21, and ES12, respectively, and investigated by several analyses. Based on the obtained results, the maximum hydrogen interactions between these two polymers, ES11 NFs offered a uniform morphology without bead with the diameter of 185.33 nm as a result of the compatibility of the polymer solutions of EC and SPI. Moreover, appropriate thermal stability was presented along with more porosity (78%), maximum water vapor transmission rate (657 g/m2.24h), good tensile stress (6.12 MPa), and acceptable water contact angel (82.3°). Therefore, ES11 NFs were selected as the optimal sample for incorporation of the BOPE as the antibacterial and antioxidant agent. According to the antioxidant activity test, the highest concentration (20% wt) of this extract increased the antioxidant activity of NF around 64.7% and also inhibited the growth of pathogenic bacteria (S. areus, and E. coli). Therefore, the ES11 electrospun NFs containing 20% BOPE can be a beneficial system to increase the safety and quality of foods.

Electrospun ethyl cellulose/poly caprolactone/gelatin nanofibers: The investigation of mechanical, antioxidant, and antifungal properties for food packaging.

The purpose of the present research was to fabricate ethylcellulose (ECL)/polycaprolactone (PCL)/gelatin (GEL) electrospun nanofibers containing Zataria multiflora essential oil (ZEO) and zinc oxide nanoparticle (ZnO) to provide an appropriate substrate for food packaging. The ECL/PCL/GEL was incorporated with ZEO and ZnO at the concentrations of 10, 20, 30 and 50 wt% and 3 wt%, respectively. The results of ECL/PCL/GEL/ZEO/ZnO nanofiber exhibited uniform morphology with a mean diameter ranging from 361.85 ± 18.7 to 467.33 ± 14.50 nm and enhanced thermal stability. The ECL/PCL/GEL/ZEO/ZnO nanofiber had the highest mechanical parameters, such as young's modulus (437.49 ± 18), tensile strength (7.88 ± 0.7), and elongation at break (5.02 ± 0.6) and water contact angle (61.13 ± 0.5), compared with the other nanofibers. The cell viability during 48 and 72 h was obtained to be about more than 80% for all the nanofibers. Additionally, the ECL/PCL/GEL incorporated with 50% ZEO and 3% ZnO displayed the highest antioxidant activity (34.61 ± 1.98%) and antifungal properties against Penicillium notatum and Aspergillus niger. In general, the ECL/PCL/GEL with the weight ratio of 20:70:10 nanofiber incorporated with 30% ZEO and 3% ZnO was obtained to have appropriate mechanical, antioxidant, and antimicrobial properties and thermal stability.

Development of resveratrol loaded chitosan-gellan nanofiber as a novel gastrointestinal delivery system.

Resveratrol loaded chitosan:gellan (CS:Gel) nanofibers (NFs) were prepared for the first time using electrospinning technique. The NFs were prepared with CS (9% w/v) in trifloroacetic acid and Gel (2.5% w/v) at a concentration ratio of 95:5% w/v. Based on the scanning electron microscopy (SEM) images, the diameters of CS-Gel fibers at a ratio of 95:5 and 90:10% w/v were 166 ±â€¯37 and 291 ±â€¯41 nm, respectively. Resveratrol (0.05% w/v) was loaded in NFs of CS-Gel at a ratio of 95:5. Encapsulation efficiency (EE) of resveratrol in NFs of CS-Gel were 86 ±â€¯6%. The amount of resveratrol delivered in intestine region was in the range of 43-51% of the total encapsulated resveratrol (equivalent to ~840 µM). Antioxidant activities of resveratrol loaded NFs were significantly higher than that of free resveratrol. Furthermore, the resveratrol releasing ability of the NFs was proved through the MTT assay and revealed that the resveratrol NFs have almost the same cytotoxicity against HT29 cancer cells compared to free resveratrol. Based on the obtained results, the prepared NFs hold great potential as drug delivery carriers for resveratrol delivery.